SSGCID: Target Page

TrcrB.00739.a

protein phosphatase 1 catalytic subunit, putative (PP1C)

CENTER ID:	TrcrB.00739.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.00739.a.B1.GE42369	full length		1	400

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.506739.130
UniProt:	Q4DRQ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRSRQKNEP DQKPDLTRCS LSTLPTETLS LNEKHDVLYR LLELLPEKYM STSPHEQRLP EALIVRVLRE AYPIIASDPI VVYVESPVCV CGDLHGQYYD LLNIFSRRPP LGGGVLGLSN HALPVQRHKR SRIYSEDSSV SHQDEFKASD APYDKYLFLG DYVDRGSRSI EVVITLLALK IIEPSKITLL RGNHEDEQIM LLYGFFDECK RRYGVKLFRI FTDLFRVLPV AAVVNGTIFC MHGGLSPELK RVNEVPDMRP CNVPHSGIIC DLLWADPEAE LSPNVDWAPS TRRISFVFSA KALEKFLKEN DLDMVCRAHQ VVEEGFQFFP SNTKRHLLTI FSATNYCNEF GNRGGVLCVD KEGVCSLLIL EPPNFHQQRM IMMKREALPN NQQNPWDMGG

NT Sequence

atgagaagaa gtcgacaaaa aaatgagccg gatcaaaaac ctgaccttac acggtgctca ttaagcacac tgcctacaga aactctctcg ttaaatgaaa agcatgatgt gctctaccgt ctactcgaac tcctacctga gaagtatatg tctacaagtc ctcatgagca acgacttcct gaagctctta ttgttcgtgt cctccgtgag gcttatccta ttattgcaag tgatccaatt gtcgtgtatg ttgaatctcc cgtttgcgta tgtggtgatt tgcatgggca gtactatgat cttttgaata tttttagccg aagaccgcct ttaggaggag gggttttggg gttgagtaat cacgctttac cggttcaacg acataaaaga agtcgaattt atagcgaaga ctcttcagtt tcgcatcaag atgagttcaa agcatccgat gccccatacg ataaatatct ttttttgggt gactatgttg accgtggtag tcgttcaatc gaagttgtga ttacattgct ggcactgaag attatagagc cgtcgaaaat aactttactg cgtggaaatc acgaagatga gcaaataatg cttctgtacg gattttttga cgaatgtaaa agacggtatg gggtaaaact gtttcgtata tttacagatc tatttcgtgt tttgccggtt gctgctgtcg ttaatggcac tattttttgt atgcatgggg gcctgtctcc agagctaaag cgtgtgaacg aagtgccaga catgcgtcca tgtaatgtcc cacatagtgg gatcatttgc gatttgctgt gggcagaccc agaggccgaa ctttcgccaa acgttgattg ggcaccaagc acacgccgaa tatcttttgt gttttccgcc aaagcacttg agaagtttct taaagaaaac gatttggata tggtttgccg ggcgcaccaa gtggtggagg aaggcttcca attttttcct agcaacacca agcgtcatct actcacaata ttttcagcca caaactattg taatgaattt ggaaaccgtg gtggggtgtt gtgcgtagac aaggaaggcg tatgtagcct tttgatattg gaacccccaa atttccatca gcagcggatg attatgatga agcgtgaagc attaccaaac aaccaacaga atccatggga tatgggtgga taa

Details for TrcrB.00739.a.B1.GE42369

HARVESTED ON:	4/13/2018
SEQUENCED ON:	4/18/2018
EXPECTED MW:	47kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMR RSRQKNEPDQ KPDLTRCSLS TLPTETLSLN EKHDVLYRLL ELLPEKYMST SPHEQRLPEA LIVRVLREAY PIIASDPIVV YVESPVCVCG DLHGQYYDLL NIFSRRPPLG GGVLGLSNHA LPVQRHKRSR IYSEDSSVSH QDEFKASDAP YDKYLFLGDY VDRGSRSIEV VITLLALKII EPSKITLLRG NHEDEQIMLL YGFFDECKRR YGVKLFRIFT DLFRVLPVAA VVNGTIFCMH GGLSPELKRV NEVPDMRPCN VPHSGIICDL LWADPEAELS PNVDWAPSTR RISFVFSAKA LEKFLKENDL DMVCRAHQVV EEGFQFFPSN TKRHLLTIFS ATNYCNEFGN RGGVLCVDKE GVCSLLILEP PNFHQQRMIM MKREALPNNQ

Validated NT Sequence

gttggttgtt tggtaatgct tcacgcttca tcataatcat ccgctgctga tggaaatttg ggggttccaa tatcaaaagg ctacatacgc cttccttgtc tacgcacaac accccaccac ggtttccaaa ttcattacaa tagtttgtgg ctgaaaatat tgtgagtaga tgacgcttgg tgttgctagg aaaaaattgg aagccttcct ccaccacttg gtgcgcccgg caaaccatat ccaaatcgtt ttctttaaga aacttctcaa gtgctttggc ggaaaacaca aaagatattc ggcgtgtgct tggtgcccaa tcaacgtttg gcgaaagttc ggcctctggg tctgcccaca gcaaatcgca aatgatccca ctatgtggga cattacatgg acgcatgtct ggcacttcgt tcacacgctt tagctctgga gacaggcccc catgcataca aaaaatagtg ccattaacga cagcagcaac cggcaaaaca cgaaatagat ctgtaaatat acgaaacagt tttaccccat accgtctttt acattcgtca aaaaatccgt acagaagcat tatttgctca tcttcgtgat ttccacgcag taaagttatt ttcgacggct ctataatctt cagtgccagc aatgtaatca caacttcgat tgaacgacta ccacggtcaa catagtcacc caaaaaaaga tatttatcgt atggggcatc ggatgctttg aactcatctt gatgcgaaac tgaagagtct tcgctataaa ttcgacttct tttatgtcgt tgaaccggta aagcgtgatt actcaacccc aaaacccctc ctcctaaagg cggtcttcgg ctaaaaatat tcaaaagatc atagtactgc ccatgcaaat caccacatac gcaaacggga gattcaacat acacgacaat tggatcactt gcaataatag gataagcctc acggaggaca cgaacaataa gagcttcagg aagtcgttgc tcatgaggac ttgtagacat atacttctca ggtaggagtt cgagtagacg gtagagcaca tcatgctttt catttaacga gagagtttct gtaggcagtg tgcttaatga gcaccgtgta aggtcaggtt tttgatccgg ctcatttttt tgtcgacttc ttctcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagaa gaagtcgaca aaaaaatgag ccggatcaaa aacctgacct tacacggtgc tcattaagca cactgcctac agaaactctc tcgttaaatg aaaagcatga tgtgctctac cgtctactcg aactcctacc tgagaagtat atgtctacaa gtcctcatga gcaacgactt cctgaagctc ttattgttcg tgtcctccgt gaggcttatc ctattattgc aagtgatcca attgtcgtgt atgttgaatc tcccgtttgc gtatgtggtg atttgcatgg gcagtactat gatcttttga atatttttag ccgaagaccg cctttaggag gaggggtttt ggggttgagt aatcacgctt taccggttca acgacataaa agaagtcgaa tttatagcga agactcttca gtttcgcatc aagatgagtt caaagcatcc gatgccccat acgataaata tctttttttg ggtgactatg ttgaccgtgg tagtcgttca atcgaagttg tgattacatt gctggcactg aagattatag agccgtcgaa aataacttta ctgcgtggaa atcacgaaga tgagcaaata atgcttctgt acggattttt tgacgaatgt aaaagacggt atggggtaaa actgtttcgt atatttacag atctatttcg tgttttgccg gttgctgctg tcgttaatgg cactattttt tgtatgcatg ggggcctgtc tccagagcta aagcgtgtga acgaagtgcc agacatgcgt ccatgtaatg tcccacatag tgggatcatt tgcgatttgc tgtgggcaga cccagaggcc gaactttcgc caaacgttga ttgggcacca agcacacgcc gaatatcttt tgtgttttcc gccaaagcac ttgagaagtt tcttaaagaa aacgatttgg atatggtttg ccgggcgcac caagtggtgg aggaaggctt ccaatttttt cctagcaaca ccaagcgtca tctactcaca atattttcag ccacaaacta ttgtaatgaa tttggaaacc gtggtggggt gttgtgcgta gacaaggaag gcgtatgtag ccttttgata ttggaacccc caaatttcca tcagcagcgg atgattatga tgaagcgtga agcattacca aacaaccaac agaatccatg ggatatgggt ggatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat