SSGCID: Target Page

TrcrB.00696.a

phenylalanyl-tRNA synthetase alpha chain, putative

CENTER ID:	TrcrB.00696.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.00696.a.B1.GE42631	full length		1	496

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.511727.140
UniProt:	Q4DY45

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTVEDFVLQ ALASTDAVES DKLAETLRVN HQDVVGAIKS LEADGYVVSE IKPKPTWKLT SEAENICESG SPEFLLWELL AGGAMTQEDI AEKFGKDVAA VAVANGMKAK IFALNKSEGK IIVTRSPSVS SFHDGIRVSL MRALKGEHVD PKEAETLKKR KLATLDNMKV FFVTKGPNYA TQRRAKAVGD LTKEMLQDGS WKTAHFKEYN LGAAGREVGC GQLHPLLKVR QEFREIFMEL GFREMETDRW VESSFWNFDA LFIAQNHPAR DVQDTFFVSK PSTHKLDHRE VVDRVREMHE KNFNYTWKEE EARRNVLRTH TTCCSGYWLH HIAKSSPLLP NGRRDFCPGR YYSIDRVFRN EEMDRTHLCE FHQVEGCVID RNISLSNMMH TFQLFFSRIG VEKLRFKPVF NPYTEPSMEI FGWHKQLKRW IEVGNSGLFR PEMLVPLGFD EDVTVMAWGL SLERPTMIKY GLSSIHELFG HKVDLRFIKQ SKLMRF

NT Sequence

atgagcactg tggaggattt tgttctgcag gcgctggcat ccacagatgc cgttgagtct gacaagttgg cggagacgtt gcgggtcaat catcaagatg ttgtgggcgc catcaagtca ctggaggcgg atggctacgt tgtgagcgaa attaagccaa aaccaacgtg gaagctgaca agtgaggcgg agaatatatg cgagagcggc agcccagagt ttcttctctg ggagttgttg gcaggtggtg caatgacaca agaagacatt gctgagaagt ttggaaaaga tgtggccgct gtggcagtag cgaatggcat gaaggctaaa atctttgctt taaataaaag tgagggtaaa atcatcgtca ctcgttcccc ttctgtctcc tcctttcacg acggcatacg cgtttcactg atgcgggccc tgaagggcga gcatgtcgat ccaaaggaag cggagacgct caagaagcgc aagctggcga cgctggacaa catgaaggtc tttttcgtga ccaagggccc aaattatgcg acacaacgac gggcaaaggc ggtgggtgat ctcacgaagg aaatgctgca ggatgggtcg tggaagacag cgcacttcaa ggagtataac cttggcgccg cgggtcgtga ggtgggctgt ggccagctgc acccactcct caaggtacgc caagagttcc gcgagatatt catggagctt ggttttcggg agatggagac ggatcgctgg gtggagtctt ccttttggaa ctttgatgcg ctctttattg cacaaaacca ccctgcccgc gatgtgcagg acactttttt tgtctccaag ccatcgaccc ataagctgga tcaccgcgag gttgtggacc gcgtgcgtga aatgcacgaa aagaacttta attacacatg gaaagaggag gaggcacggc gtaatgtgct gcgcacgcac acaacttgct gttccggcta ttggctccat cacattgcga aatcgtcccc gttgctaccg aacggacgcc gtgatttttg ccctggtcgc tactacagca ttgaccgtgt cttccgcaat gaggagatgg accgcacaca tctttgtgag ttccaccaag ttgagggctg cgtcattgac cgtaacatct ccctatcgaa tatgatgcac acttttcagc ttttctttag tcggattgga gtagagaagt tacgcttcaa gcctgtcttc aacccgtaca cggagccgtc aatggaaatc tttggctggc acaagcagct gaagcggtgg attgaagtgg gcaacagcgg cctcttccgc cccgagatgc ttgtgccgct gggctttgac gaggacgtca cggtgatggc gtggggactg tcgctcgaac ggccaacgat gatcaagtac ggcctgtcga gtatccacga gctctttggt cacaaggttg atctgcgctt tatcaaacaa tccaaactga tgcgtttcta a

Details for TrcrB.00696.a.B1.GE42631

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	92
PERCENT COVERAGE:	57

Validated AA Sequence

MAHHHHHHMS TVEDFVLQAL ASTDAVESDK LAETLRVNHQ DVVGAIKSLE SDGYVVSEIK PKPTWKLTSE AEIICESGSP EFLLWELLAG GAMTQEDIAE KFGKDVAAVA VANGMKAKIF ALNKSEGKII VTRSPSVSSF HDGIRVSLMR ALKGEHVDPK EAETLKKRKL ATLDNMKVFF VTKGPNYATQ RRAKAVGDLT KEMLQDGSWK TTHFKEYNLG AAGREVGCGQ LHPLLKVRQE FREIFMELGF REMETDRWVE SSFWNFDALF XCTKPPCPRC AGHFFCLQAI DP

Validated NT Sequence

ttgannaagc gcagatcaac cttgtgacca aagagctcgt ggatactcga caggccgtac ttgatcatcg ttggccgttc gagcgacagt ccccacgcca tcaccgtgac gtcctcgtca aagcccagcg gcacaagcat ctcggggcgg aagaggccgc tgttgcccac ttcaatccac cgcttcagct gcttgtgcca gccaaagatt tccattgacg gctccgtgta cgggttgaag acaggcttga agcgtaactt ctctactcca atccgactaa agaaaagctg aaaagtgtgc atcatattcg atagggagat gttacggtca atgacgcagc cctcaacttg gtggaactca caaagatgtg tgcggtccat ctcctcattg cggaagacac ggtcaatgct gtagtagcga ccagggcaaa aatcacggcg tccgttcggt agcaacgggg acgatttcgc aatgtgatgg agccaatagc cggaacagca agttgtgtgc gtgcgcagca cattacgccg tgcctcctcc tctttccatg tgtaattaaa gttcttttcg tgcatttcac gcacgcggtc cacaacctcg cggtgatcca gcttatgggt cgatggcttg gagacaaaaa aagtgtcctg cacatcgcgg gcagggtggt tttgtgcaat naaanagcgc atcaaagttc caaaaggaag actccaccca gcgatccgtc tccatctccc gaaaaccaag ctccatgaat atctcgcgga actcttggcg taccttgagg agtgggtgca gctggccaca gcccacctca cgacccgcgg cgccaaggtt atactccttg aagtgcgttg tcttccacga cccatcctgc agcatttcct tcgtgagatc acccaccgcc tttgcccgtc gttgtgtcgc ataatttggg cccttggtca cgaaaaagac cttcatgttg tccagcgtcg ccagcttgcg cttcttgagc gtctccgctt cctttggatc gacatgctcg cccttcaggg cccgcatcag tgaaacgcgt atgccgtcgt gaaaggagga gacagaaggg gaacgagtga cgatgatttt accctcactt ttatttaaag caaagatttt agccttcatg ccattcgcta ctgccacagc ggccacatct tttccaaact tctccgcaat gtcttcttgt gtcattgcac cacctgccaa caactcccag agaagaaact ctgggctgcc gctctcgcat ataatctccg cctcacttgt caacttccac gttggttttg gcttaatttc gctcacaacg tagccatccg actccagtga cttgatggcg cccacaacat cttgatgatt gacccgcaac gtctccgcca acttgtcaga ctcaacggca tctgtggatg ccagcgcctg cagaacaaaa tcctccacag tgctcatatg gtggtggtgg tggtgagcca tggtatatct

Expected Protein Sequence

MAHHHHHHMS TVEDFVLQAL ASTDAVESDK LAETLRVNHQ DVVGAIKSLE ADGYVVSEIK PKPTWKLTSE AENICESGSP EFLLWELLAG GAMTQEDIAE KFGKDVAAVA VANGMKAKIF ALNKSEGKII VTRSPSVSSF HDGIRVSLMR ALKGEHVDPK EAETLKKRKL ATLDNMKVFF VTKGPNYATQ RRAKAVGDLT KEMLQDGSWK TAHFKEYNLG AAGREVGCGQ LHPLLKVRQE FREIFMELGF REMETDRWVE SSFWNFDALF IAQNHPARDV QDTFFVSKPS THKLDHREVV DRVREMHEKN FNYTWKEEEA RRNVLRTHTT CCSGYWLHHI AKSSPLLPNG RRDFCPGRYY SIDRVFRNEE MDRTHLCEFH QVEGCVIDRN ISLSNMMHTF QLFFSRIGVE KLRFKPVFNP YTEPSMEIFG WHKQLKRWIE VGNSGLFRPE MLVPLGFDED VTVMAWGLSL ERPTMIKYGL SSIHELFGHK VDLRFIKQSK LMRF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca ctgtggagga ttttgttctg caggcgctgg catccacaga tgccgttgag tctgacaagt tggcggagac gttgcgggtc aatcatcaag atgttgtggg cgccatcaag tcactggagg cggatggcta cgttgtgagc gaaattaagc caaaaccaac gtggaagctg acaagtgagg cggagaatat atgcgagagc ggcagcccag agtttcttct ctgggagttg ttggcaggtg gtgcaatgac acaagaagac attgctgaga agtttggaaa agatgtggcc gctgtggcag tagcgaatgg catgaaggct aaaatctttg ctttaaataa aagtgagggt aaaatcatcg tcactcgttc cccttctgtc tcctcctttc acgacggcat acgcgtttca ctgatgcggg ccctgaaggg cgagcatgtc gatccaaagg aagcggagac gctcaagaag cgcaagctgg cgacgctgga caacatgaag gtctttttcg tgaccaaggg cccaaattat gcgacacaac gacgggcaaa ggcggtgggt gatctcacga aggaaatgct gcaggatggg tcgtggaaga cagcgcactt caaggagtat aaccttggcg ccgcgggtcg tgaggtgggc tgtggccagc tgcacccact cctcaaggta cgccaagagt tccgcgagat attcatggag cttggttttc gggagatgga gacggatcgc tgggtggagt cttccttttg gaactttgat gcgctcttta ttgcacaaaa ccaccctgcc cgcgatgtgc aggacacttt ttttgtctcc aagccatcga cccataagct ggatcaccgc gaggttgtgg accgcgtgcg tgaaatgcac gaaaagaact ttaattacac atggaaagag gaggaggcac ggcgtaatgt gctgcgcacg cacacaactt gctgttccgg ctattggctc catcacattg cgaaatcgtc cccgttgcta ccgaacggac gccgtgattt ttgccctggt cgctactaca gcattgaccg tgtcttccgc aatgaggaga tggaccgcac acatctttgt gagttccacc aagttgaggg ctgcgtcatt gaccgtaaca tctccctatc gaatatgatg cacacttttc agcttttctt tagtcggatt ggagtagaga agttacgctt caagcctgtc ttcaacccgt acacggagcc gtcaatggaa atctttggct ggcacaagca gctgaagcgg tggattgaag tgggcaacag cggcctcttc cgccccgaga tgcttgtgcc gctgggcttt gacgaggacg tcacggtgat ggcgtgggga ctgtcgctcg aacggccaac gatgatcaag tacggcctgt cgagtatcca cgagctcttt ggtcacaagg ttgatctgcg ctttatcaaa caatccaaac tgatgcgttt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat