TrcrB.00612.b

lysyl-tRNA synthetase, putative

CENTER ID: TrcrB.00612.b
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.00612.b.B1.GE42630 full length 1 580
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.508971.30
UniProt: Q4D7V3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSTNETRAQ IDDLAAAIAR VKKEKGAASE ECKALVAKMT ELRKQLPAKK VEKAPELSYF DTRLAMVKEL GLLGAAYPHK FDRQYTIPAF KARFAPQLSE KGQRVEEVVA IAGRIVNKRS SGSKLNFLTL QGDADTVQVI SAISDYVDDT FAAVHGRIRR GDIIGVKGVA SLSKTGEFSM NAFEITLLST CYHMLPDGWY GLSSIEQRFR QRYLDFIVNR ENIQTFVTRS KVIRYIRNFF EDLNFLEVET PVLNQIAGGA AARPFITHHN ELNQRMYLRI APELYLKELV VGGMDRVYEL GKQFRNEGID LTHNPEFTSV EAYWAYADYN DWMRTTEDLF YGLAMHIHGT PFVKYAPKDS EGNQLAEVVF NFNKPFKRLY IIPELEKRTN VKFPTEFESD SSNAFLQELC SKHEVECTPP LTTARLLDAL ISHYLEPECQ DPTFVCDHPR VMSPLAKWHR NDPQLTERFE LFLNKKELCN AYTELNNPIV QREEFMKQLR NKEKGDDEAM DIDEGFVQAL EHALPPTGGW GLGIDRLVMF LTSQANIKEV LLFPAMKPET SSSLTYPPGT LLNGQGVPLL
NT Sequence
atgtcatcca cgaatgagac gcgcgcccag attgacgatc tcgccgcggc gattgcgcgg gtaaagaagg agaagggggc tgccagcgag gagtgtaagg ccctcgttgc caaaatgacg gagctgcgga agcaactccc tgcgaagaag gtggagaagg cgccggagct cagttacttc gacacgcggc tggcgatggt gaaggagtta gggctcctcg gcgccgcgta cccgcacaag tttgatcgtc agtacacgat acctgccttc aaggcgcgtt ttgccccgca gcttagcgaa aaagggcaac gtgtcgagga ggtcgtggcg attgccggcc ggattgtcaa caagcgaagc tcgggctcga agctcaactt tttgacgctg cagggggacg ccgacaccgt gcaggtgatt tcagccatca gcgactacgt ggatgacacg tttgccgccg ttcacggcag gatcaggcgt ggcgacatca tcggcgtgaa gggcgtcgca tcgctctcaa agacagggga gttttccatg aacgcgtttg agatcaccct gctatcgaca tgttaccaca tgctgcccga tggctggtac gggctgagct caattgagca gcgcttccgc caacggtacc tggactttat tgtgaatcgc gagaacatac aaacgtttgt cacgcgctct aaagtcatac gatacattcg gaattttttt gaagacttga actttcttga ggtggagacg ccggtgctga accaaatcgc cggtggtgcc gccgcccgtc cattcatcac gcaccacaac gaattgaacc agaggatgta cctgcgcatt gctcccgagt tgtacttgaa ggaacttgtt gtgggcggga tggaccgcgt gtacgaactc ggcaagcagt tcaggaatga gggcattgac ctcacacaca acccggagtt tacaagtgtc gaggcgtact gggcctacgc cgactacaat gattggatgc gcaccacgga ggatctcttc tacggactcg ccatgcacat tcacggcaca ccgttcgtca agtatgcccc caaggactca gagggcaatc aacttgctga ggtggtcttt aacttcaaca aaccctttaa gcgcctgtac atcattccgg agctggagaa gcggacgaac gttaagttcc ccaccgagtt cgagagcgac tcatccaacg catttctgca ggaattgtgc agtaaacacg aggtggagtg cacccccccg cttacaacgg cacgcctgtt ggatgcgttg atttcccact acttggagcc ggagtgccag gacccgactt ttgtctgcga tcacccacgt gtcatgtcgc ccctcgccaa gtggcaccgt aatgaccctc aactaacaga acgatttgaa ctctttttga acaagaagga gctctgcaac gcgtacaccg agcttaacaa cccaatagtg cagcgggagg agttcatgaa gcagcttcgg aacaaagaaa agggcgacga cgaggcgatg gatatcgacg aggggtttgt tcaggcactg gagcacgcat tgcccccgac aggcggctgg gggctgggta ttgaccgtct cgttatgttc ctcactagcc aggcgaacat caaggaggtt cttctctttc ctgccatgaa gccggagacc tcgagctccc tcacgtaccc acccggcacg ctacttaacg gtcagggcgt acctctcctc taa
Details for TrcrB.00612.b.B1.GE42630
HARVESTED ON: 8/5/2018
SEQUENCED ON: 8/8/2018
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS STNETRAQID DLAAAIARVK KEKGAASEEC KALVAKMTEL RKQLPAKKVE KAPELSYFDT RLAMVKELGL LGAAYPHKFD RQYTIPAFKA RFAPQLSEKG QRVEEVVAIA GRIVNKRSSG SKLNFLTLQG DADTVQVISA ISDYVDDTFA AVHGRIRRGD IIGVKGVASL SKTGEFSMNA FEITLLSTCY HMLPDGWYGL SSIEQRFRQR YLDFIVNREN IQTFVTRSKV IRYIRNFFED LNFLEVETPV LNQIAGGAAA RXFITHHNEL NQRMYLRIAP ELYLKELVVG GMDRVYELGK QFRNEGIDLT HNPEFTSVEA YWAYADYNDW MRTTEDLFYG LAMHIHXTPF VKYVPKDSEG NQLAEVVFNF NKPFKRLYII PELEKRTNVK FPTEFESDSS NAFLQELCSK HEVECTPPLT TARLLDALIS HYLEPECQDP TFVCDHPRVM SPLAKWHRND PQLTERFELF LNKKELCNAY TELNNPIVQR EEFMKQLRNK EKGDDEAMDI DEGFVQALEH ALPPTGGWGL GIDRLVMFLT SQANIKEVLL FPAMKPETSS SLTYPPGTLL XGQGVPLL
Validated NT Sequence
taagtagcgt gccgggtggg tacgtgaggg agctcgaggt ctccggcttc atggcaggaa agagaagaac ctccttgatg ttcgcctggc tagtgaggaa cataacgaga cggtcaatac ccagccccca gccgcctgtc gggggcaatg cgtgctccag tgcctgaaca aacccctcgt cgatatccat cgcctcgtcg tcgccctttt ctttgttccg aagctgcttc atgaactcct cccgctgcac tattgggttg ttaagctcgg tgtacgcgtt gcagagctcc ttcttgttca aaaagagttc aaatcgttct gttagttgag ggtcattacg gtgccacttg gcgaggggcg acatgacacg tgggtgatcg cagacaaaag tcgggtcctg gcactccggc tccaagtagt gggaaatcaa cgcatccaac aggcgtgccg ttgtaagcgg gggggtgcac tccacctcgt gtttactgca caattcctgc agaaatgcgt tggatgagtc gctctcgaac tcggtgggga acttaacgtt cgtccgcttc tccagctccg gaatgatgta caggcgctta aagggtttgt tgaagttaaa gaccacctca gcaagttgat tgccctctga gtccttnnng acatacttga cgaacggtgt gnngtgaatg tgcatggcga gtccgtagaa gagatcctcc gtggtgcgca tccaatcatt gtagtcggcg taggcccagt acgcctcgnn ncttgtaaac tccgggnnnt gtgtgaggtc aatnnnnnna nnnnngaact gcttgcnnnn nnnnngtaca cgcggtccnn ncccnnnnnn nnnnnnnnnn nnnnnnnacn nnnnnnnnnn nnnnnncnnn nnnnnnnnnn ngnnnnnnnn nnnnnnnntg nntgatgaan nnnnnnnnnn nnnnnnnnnn nnncgannnn nnncnnnann nnnntctcca cctcaagaaa nnnnaagtct ncnnnaannn nnngaatgta tcgtatnnnt ttagagcgcg tgacaaacgn nngtatgttc tcgcgattca caataaagtc caggtaccgt tggcggaagc gctgctcaat tgagctcagc ccgtaccagc catcgggcag catgtggtaa catgtcgata gcagggtgat ctcaaacgcg ttcatggaaa actcccctgt ctttgagagc gatgcgacgc ccttcacgcc gatgatgtcg ccacgcctga tcctgccgtg aacggcggca aacgtgtcat ccacgtagtc gctgatggct gaaatcacct gcacggtgtc ggcgtccccc tgcagcgtca aaaagttgag cttcgagccc gagcttcgct tgttgacaat ccggccggca atcgccacga cctcctcgac acgttgccct ttttcgctaa gctgcggggc aaaacgcgcc ttgaaggcag gtatcgtgta ctgacgatca aacttgtgcg ggtacgcggc gccgaggagc cctaactcct tcaccatcgc cagccgcgtg tcgaagtaac tgagctccgg cgccttctcc accttcttcg cagggagttg cttccgcagc tccgtcattt tggcaacgag ggccttacac tcctcgctgg cagccccctt ctccttcttt acccgcgcaa tcgccgcggc gagatcgtca atctgggcgc gcgtctcatt cgtggatgac atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMS STNETRAQID DLAAAIARVK KEKGAASEEC KALVAKMTEL RKQLPAKKVE KAPELSYFDT RLAMVKELGL LGAAYPHKFD RQYTIPAFKA RFAPQLSEKG QRVEEVVAIA GRIVNKRSSG SKLNFLTLQG DADTVQVISA ISDYVDDTFA AVHGRIRRGD IIGVKGVASL SKTGEFSMNA FEITLLSTCY HMLPDGWYGL SSIEQRFRQR YLDFIVNREN IQTFVTRSKV IRYIRNFFED LNFLEVETPV LNQIAGGAAA RPFITHHNEL NQRMYLRIAP ELYLKELVVG GMDRVYELGK QFRNEGIDLT HNPEFTSVEA YWAYADYNDW MRTTEDLFYG LAMHIHGTPF VKYAPKDSEG NQLAEVVFNF NKPFKRLYII PELEKRTNVK FPTEFESDSS NAFLQELCSK HEVECTPPLT TARLLDALIS HYLEPECQDP TFVCDHPRVM SPLAKWHRND PQLTERFELF LNKKELCNAY TELNNPIVQR EEFMKQLRNK EKGDDEAMDI DEGFVQALEH ALPPTGGWGL GIDRLVMFLT SQANIKEVLL FPAMKPETSS SLTYPPGTLL NGQGVPLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcat ccacgaatga gacgcgcgcc cagattgacg atctcgccgc ggcgattgcg cgggtaaaga aggagaaggg ggctgccagc gaggagtgta aggccctcgt tgccaaaatg acggagctgc ggaagcaact ccctgcgaag aaggtggaga aggcgccgga gctcagttac ttcgacacgc ggctggcgat ggtgaaggag ttagggctcc tcggcgccgc gtacccgcac aagtttgatc gtcagtacac gatacctgcc ttcaaggcgc gttttgcccc gcagcttagc gaaaaagggc aacgtgtcga ggaggtcgtg gcgattgccg gccggattgt caacaagcga agctcgggct cgaagctcaa ctttttgacg ctgcaggggg acgccgacac cgtgcaggtg atttcagcca tcagcgacta cgtggatgac acgtttgccg ccgttcacgg caggatcagg cgtggcgaca tcatcggcgt gaagggcgtc gcatcgctct caaagacagg ggagttttcc atgaacgcgt ttgagatcac cctgctatcg acatgttacc acatgctgcc cgatggctgg tacgggctga gctcaattga gcagcgcttc cgccaacggt acctggactt tattgtgaat cgcgagaaca tacaaacgtt tgtcacgcgc tctaaagtca tacgatacat tcggaatttt tttgaagact tgaactttct tgaggtggag acgccggtgc tgaaccaaat cgccggtggt gccgccgccc gtccattcat cacgcaccac aacgaattga accagaggat gtacctgcgc attgctcccg agttgtactt gaaggaactt gttgtgggcg ggatggaccg cgtgtacgaa ctcggcaagc agttcaggaa tgagggcatt gacctcacac acaacccgga gtttacaagt gtcgaggcgt actgggccta cgccgactac aatgattgga tgcgcaccac ggaggatctc ttctacggac tcgccatgca cattcacggc acaccgttcg tcaagtatgc ccccaaggac tcagagggca atcaacttgc tgaggtggtc tttaacttca acaaaccctt taagcgcctg tacatcattc cggagctgga gaagcggacg aacgttaagt tccccaccga gttcgagagc gactcatcca acgcatttct gcaggaattg tgcagtaaac acgaggtgga gtgcaccccc ccgcttacaa cggcacgcct gttggatgcg ttgatttccc actacttgga gccggagtgc caggacccga cttttgtctg cgatcaccca cgtgtcatgt cgcccctcgc caagtggcac cgtaatgacc ctcaactaac agaacgattt gaactctttt tgaacaagaa ggagctctgc aacgcgtaca ccgagcttaa caacccaata gtgcagcggg aggagttcat gaagcagctt cggaacaaag aaaagggcga cgacgaggcg atggatatcg acgaggggtt tgttcaggca ctggagcacg cattgccccc gacaggcggc tgggggctgg gtattgaccg tctcgttatg ttcctcacta gccaggcgaa catcaaggag gttcttctct ttcctgccat gaagccggag acctcgagct ccctcacgta cccacccggc acgctactta acggtcaggg cgtacctctc ctctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat