SSGCID: Target Page

TrcrB.00424.a

Protein kinase, putative (EC 2.7.1.-)

CENTER ID:	TrcrB.00424.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.00424.a.B2.GE43848	kinase domain (Jensen)		1	337

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tc00.1047053509231.20
UniProt:	Q4D0S5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MENYEVLNQL GDGAFGQVVK ALHRKTGQIV AIKKMKQKYH SWEECVKLPE VTVMRRIHGH PNIVKMREVI REKNELFFVF EYMDGDLLGV ICKAKQMQGH TTAGNAPPIP YPKIRSYIFQ LLQSLAFLHQ RGYFHRDIKP ENLLVKKDPT TAAQDVVKLA DFGLVKEIRA RPPFTDYVST RWYRAPELLL QDRAYSSPVD IWAAGCILAE LITTRPLFAG SNEVDQLHKI MGVLGSPNEK IWPECFALAK KIRYSFPVVK GVGLERVLPP HLPPQALDLM KQMLSYDPKK RPTAQQCLQH PYFSIGLDEE NFSPSNVAKQ LANAVKKYVS SPQTAPPRNT LVPIALNAKR SNVGATTAIS KQAGVGNPPP LSPLQALCSP TATGLSPVLL GEKGDHHLPV SKLTPAIGSE GHTKRKVLSP KLPSLSCEKL KGVVTDVLNG GDGGNNNGDV DLDALTKEFE NELETLKVLP QQKKQVSLCT LGDSAANKAF HNPTFGKSLL VPGATHASPK VNEDVVVDFL NSTRYKRSSL SLMENGGPLH TTRKASLKKQ DPLPSLPPAL DSSHHAVSPS IKALLAKHMK KRNDSNS

NT Sequence

atggagaact acgaggtcct caatcaacta ggtgatggag cgtttggtca ggttgtgaag gcattgcata gaaaaacggg acaaattgtg gcgattaaaa aaatgaagca gaagtaccat tcctgggagg agtgtgtaaa gctgccagag gtgacagtga tgcgccgcat tcatggccat ccgaatatag tgaagatgcg tgaagtgata cgagaaaaaa atgagctttt ctttgtattt gagtacatgg acggggacct tctgggggtc atctgcaagg caaagcaaat gcagggccac accacagccg gcaatgcccc tcccatacca tacccgaaga ttcgaagtta catttttcag cttcttcaat cccttgcatt cttgcatcaa cgtgggtact ttcaccgaga cattaaacct gaaaatttgc ttgtgaagaa ggacccaacc accgcggcac aggatgtggt aaaactggca gattttggcc tcgtgaagga gattcgggca cgcccaccgt ttacggatta tgtttccacc cgatggtacc gcgctcctga gttgttgctg caagatcgag cgtacagcag ccctgtggat atatgggccg ctggttgtat attagcagaa cttataacca ctcgtcctct ttttgcaggt agcaatgagg tggatcagtt acacaagata atgggtgttc ttggctcacc caatgaaaaa atctggccag aatgttttgc tcttgccaag aaaatacggt attcatttcc agtagtgaag ggggtgggac ttgagcgtgt gcttccccca catttaccac cgcaagcctt ggatttaatg aagcagatgc ttagttacga ccccaagaaa cgtcccacgg cccaacagtg tcttcaacat ccatatttta gtattggtct cgatgaggag aatttttcac cttccaacgt ggcgaaacag ttggcgaacg cggtgaagaa gtatgtctct agtccacaaa ccgctccgcc acggaacacg cttgtaccta ttgctctcaa tgcaaagcga tccaatgtag gggccacgac ggccatttca aaacaggcgg gagtggggaa tcccccaccc ctgtcaccgc tgcaggcgtt gtgttccccc acagccacag gtctgtcacc cgtgttgctt ggcgagaagg gagaccacca tttaccggta tcgaaattga cgcctgcaat cgggtctgag ggccatacga aacgaaaagt tctttcgcca aaacttccct ctttgtcttg tgaaaaactt aagggtgttg tgacagatgt tttgaatggc ggtgatgggg ggaataataa cggcgatgtc gatttagatg ctcttacgaa ggagtttgaa aatgaacttg aaacactgaa agtccttcca caacagaaaa aacaagttag cctctgcacg cttggtgact ctgcggcaaa caaggctttt cataatccaa catttgggaa atccctttta gttccggggg cgacccatgc ttcaccgaag gtgaacgagg atgttgtagt agattttctt aattctactc gttacaagag gtcttcgttg tccctcatgg aaaatggtgg tccgttgcat acaacgagaa aggcctcttt gaagaaacaa gatcccctac cttcattgcc tccagcatta gacagtagtc accatgctgt atcaccatcc atcaaggcac tcttggcaaa acacatgaag aaacggaatg actccaattc ctga

Details for TrcrB.00424.a.B2.GE43848

HARVESTED ON:	8/24/2020
SEQUENCED ON:	8/28/2020
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHME NYEVLNQLGD GAFGQVVKAL HRKTGQIVAI KKMKQKYHSW EECVKLPEVT VMRRIHGHPN IVKMREVIRE KNELFFVFEY MDGDLLGVIC KAKQMQGHTT AGNAPPIPYP KIRSYIFQLL QSLAFLHQRG YFHRDIKPEN LLVKKDPTTA AQDVVKLADF GLVKEIRARP PFTDYVSTRW YRAPELLLQD RAYSSPVDIW AAGCILAELI TTRPLFAGSN EVDQLHKIMG VLGSPNEKIW PECFALAKKI RYSFPVVKGV GLERVLPPHL PPQALDLMKQ MLSYDPKKRP TAQQCLQHPY FSIGLDEENF SPSNVAKQLA NAVKKYVXSP QTA

Validated NT Sequence

atggctcacc accaccacca ccatatggag aactacgagg tcctcaatca actaggtgat ggagcgtttg gtcaggttgt gaaggcattg catagaaaaa cgggacaaat tgtggcgatt aaaaaaatga agcagaagta ccattcctgg gaggagtgtg taaagctgcc agaggtgaca gtgatgcgcc gcattcatgg ccatccgaat atagtgaaga tgcgtgaagt gatacgagaa aaaaatgagc ttttctttgt atttgagtac atggacgggg accttctggg ggtcatctgc aaggcaaagc aaatgcaggg ccacaccaca gccggcaatg cccctcccat accatacccg aagattcgaa gttacatttt tcagcttctt caatcccttg cattcttgca tcaacgtggg tactttcacc gagacattaa acctgaaaat ttgcttgtga agaaggaccc aaccaccgcg gcacaggatg tggtaaaact ggcagatttt ggnctcgtga aggagattcg ggcacgccca ccgtttacgg attatgtttc cacccgatgg taccgcgctc ctgagttgtt gctgcaagat cgagcgtaca gcagccctgt ggatatatgg gccgctggtt gtatattagc agaacttata accactcgtc ctctttttgc aggtagcaat gaggtggatc agttacacaa gataatgggt gttcttggct cacccaatga aaaaatctgg ccagaatgtt ttgctcttgc caagaaaata cggtattcat ttccagtagt gaagggggtg ggacttgagc gtgtgcttcc cccacattta ccaccgcaag ccttggattt aatgaagcag atgcttagtt acgaccccaa gaaacgtccc acggcccaac agtgtcttca acatccatat tttagtattg gtctcgatga ggagaatttt tcaccttcca acgtggcgaa acagttggcg aacgcggtga agaagtatgt ctntagtcca caaaccgctc c

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaga actacgaggt cctcaatcaa ctaggtgatg gagcgtttgg tcaggttgtg aaggcattgc atagaaaaac gggacaaatt gtggcgatta aaaaaatgaa gcagaagtac cattcctggg aggagtgtgt aaagctgcca gaggtgacag tgatgcgccg cattcatggc catccgaata tagtgaagat gcgtgaagtg atacgagaaa aaaatgagct tttctttgta tttgagtaca tggacgggga ccttctgggg gtcatctgca aggcaaagca aatgcagggc cacaccacag ccggcaatgc ccctcccata ccatacccga agattcgaag ttacattttt cagcttcttc aatcccttgc attcttgcat caacgtgggt actttcaccg agacattaaa cctgaaaatt tgcttgtgaa gaaggaccca accaccgcgg cacaggatgt ggtaaaactg gcagattttg gcctcgtgaa ggagattcgg gcacgcccac cgtttacgga ttatgtttcc acccgatggt accgcgctcc tgagttgttg ctgcaagatc gagcgtacag cagccctgtg gatatatggg ccgctggttg tatattagca gaacttataa ccactcgtcc tctttttgca ggtagcaatg aggtggatca gttacacaag ataatgggtg ttcttggctc acccaatgaa aaaatctggc cagaatgttt tgctcttgcc aagaaaatac ggtattcatt tccagtagtg aagggggtgg gacttgagcg tgtgcttccc ccacatttac caccgcaagc cttggattta atgaagcaga tgcttagtta cgaccccaag aaacgtccca cggcccaaca gtgtcttcaa catccatatt ttagtattgg tctcgatgag gagaattttt caccttccaa cgtggcgaaa cagttggcga acgcggtgaa gaagtatgtc tctagtccac aaaccgctcc gccatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat