TrcrB.00251.a

serine/threonine-protein kinase a, putative (RDK2)

CENTER ID: TrcrB.00251.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.00251.a.B2.GE43002 PHoranyi kinase domain truncation 96 504
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.507897.30
UniProt: Q4CXF6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVQSRWAER SEWKKFDCTV MFQSFLFVFV FVFVFYVCVW CTCMYSPLLS ISLSLPLCSV IVRTMTDTKR MANANSGLPQ PPAEGDAAKS DPLEGILGTH PDCKYIKKKL LGQGSFGSAW RVEERETGTI YAAKVMDTNN MSSKDRGFVT NEVKCLSRCN NANIIRHHVS YERGGMLLII MEYADGGDLY KQIKARQQST RYFKEHEVLF IFLQLCLALD HIHMNKMMHR DLKTANVLLT TTGLVKLGDF GFSRQYEDSL SNPVGSTFCG TPYYLSPELW RRAPYSKKSE MWALGVVLYE VIVLKRPFGG RNMDELIDNI LHARRQPLPN IYSEDLRNVC DQLLSLDPKY RPSLRQLFQQ PFIRKGLETL RRSVLNHNRI PQNVQKDIAC NVDEVLNSDI QDYESSRVVP RRGIVTRHTA DRGWQDCELL LDEEAVVMRD VATGSEERVE LNALTSVCPI DAAIAHEKFV FAMKNQTGKA FWFKEVNETS YEEWLVALQN ALPF
NT Sequence
atgcgcgttc aaagccgttg ggcggaaagg agtgaatgga agaaatttga ctgcactgtc atgtttcaat ccttcctttt tgtttttgtt tttgtttttg ttttttatgt ctgtgtgtgg tgtacatgta tgtattctcc cctgctgtct atctctctct ctctccctct ctgctcagtg atagtgagga caatgaccga cacaaaacgg atggcaaatg ccaatagcgg cctgccgcaa ccccccgccg aaggtgatgc cgcgaagtcc gacccattgg agggcattct tggcacgcat ccggattgca aatatataaa gaagaagtta ctgggccagg gcagttttgg cagcgcgtgg cgtgtggagg aacgggaaac agggacgatc tacgcggcga aagtcatgga caccaacaac atgagctcca aggatcgcgg ttttgtaaca aacgaagtaa agtgcctctc gcgctgcaac aatgcgaaca tcatacgaca tcacgtttct tacgaacggg gtgggatgct tctgatcatc atggaatacg ctgacggtgg agatctctac aagcaaatta aagcacgaca gcagtccaca cggtatttta aggagcacga ggtcctattt attttcctac agctctgtct tgcccttgac cacatccaca tgaacaagat gatgcacagg gacctgaaaa cagccaatgt ccttcttacc accacagggc tggtgaagct gggcgatttt ggtttctccc ggcagtatga ggactcgctc tccaacccag tcggctccac attctgcggc acgccgtact acttgagccc ggagctctgg cgccgcgcac catacagcaa gaagagtgag atgtgggcgc ttggtgttgt gttgtatgaa gttattgtcc tcaaacgccc ctttggcggc cgtaacatgg acgagctcat tgacaacatt ctgcacgccc gccgccagcc gcttccaaat atttacagtg aagatctacg caacgtgtgc gaccagctgc tctcgcttga cccgaagtat cgcccgtctc ttcgacagtt gtttcaacag ccgtttatcc gcaaagggct tgagacgctg cgtcgtagtg tattgaacca caaccgcatt ccgcagaatg tgcagaagga cattgcctgc aacgtggacg aggtgctcaa ctccgacatt caagactacg agagcagtcg tgtggtgccg cggcgaggga ttgtcacccg gcacacggca gatcgcggct ggcaggattg cgaacttttg ttggacgagg aggcggtcgt tatgcgggat gtggcgactg gctcggagga gcgtgttgaa ctgaacgccc tgacatccgt ttgtcccatt gacgccgcga ttgcgcatga aaagtttgtc tttgcgatga aaaaccaaac tgggaaggcc ttttggttca aagaagtcaa cgagacgtcg tacgaggagt ggcttgtcgc tctgcagaac gccttgccat tttaa
Details for TrcrB.00251.a.B2.GE43002
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHIL GTHPDCKYIK KKLLGQGSFG SAWRVEERET GTIYAAKVMD TNNMSSKDRG FVTNEVKCLS RCNNANIIRH HVSYERGGML LIIMEYADGG DLYKQIKARQ QSTRYFKEHE VLFIFLQLCL ALDHIHMNKM MHRDLKTANV LLTTTGLVKL GDFGFSRQYE DSLSNPVGST FCGTPYYLSP ELWRRAPYSK KSEMWALGVV LYEVIVLKRP FGGRNMDELI DNILHARRQP LPNIYSEDLR NVCDQLLSLD PKYRPSLRQL FQQPFIRKGL ETLRRSVLNH NRIPQNVQKD IACNVDEVLN SDIQDYESSR VVPRRGIVTR HTADRGWQDC ELLLDEEAVV MRDVATGSEE RVELNALTSV CPIDAAIAHE KFVFAMKNQT GKAFWFKEVN ETSYEEWLVA LQNALPF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattcttg gcacgcatcc ggattgcaaa tatataaaga agaagttact gggccagggc agttttggca gcgcgtggcg tgtggaggaa cgggaaacag ggacgatcta cgcggcgaaa gtcatggaca ccaacaacat gagctccaag gatcgcggtt ttgtaacaaa cgaagtaaag tgcctctcgc gctgcaacaa tgcgaacatc atacgacatc acgtttctta cgaacggggt gggatgcttc tgatcatcat ggaatacgct gacggtggag atctctacaa gcaaattaaa gcacgacagc agtccacacg gtattttaag gagcacgagg tcctatttat tttcctacag ctctgtcttg cccttgacca catccacatg aacaagatga tgcacaggga cctgaaaaca gccaatgtcc ttcttaccac cacagggctg gtgaagctgg gcgattttgg tttctcccgg cagtatgagg actcgctctc caacccagtc ggctccacat tctgcggcac gccgtactac ttgagcccgg agctctggcg ccgcgcacca tacagcaaga agagtgagat gtgggcgctt ggtgttgtgt tgtatgaagt tattgtcctc aaacgcccct ttggcggccg taacatggac gagctcattg acaacattct gcacgcccgc cgccagccgc ttccaaatat ttacagtgaa gatctacgca acgtgtgcga ccagctgctc tcgcttgacc cgaagtatcg cccgtctctt cgacagttgt ttcaacagcc gtttatccgc aaagggcttg agacgctgcg tcgtagtgta ttgaaccaca accgcattcc gcagaatgtg cagaaggaca ttgcctgcaa cgtggacgag gtgctcaact ccgacattca agactacgag agcagtcgtg tggtgccgcg gcgagggatt gtcacccggc acacggcaga tcgcggctgg caggattgcg aacttttgtt ggacgaggag gcggtcgtta tgcgggatgt ggcgactggc tcggaggagc gtgttgaact gaacgccctg acatccgttt gtcccattga cgccgcgatt gcgcatgaaa agtttgtctt tgcgatgaaa aaccaaactg ggaaggcctt ttggttcaaa gaagtcaacg agacgtcgta cgaggagtgg cttgtcgctc tgcagaacgc cttgccattt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat