TrcrA.19097.a

Uncharacterized protein

CENTER ID: TrcrA.19097.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.19097.a.B1.GE39112 full length 1 469
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:TCSYLVIO_002855
UniProt: K4E6F9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRQGMVEGEM QESEVPHASH EAGEEESSAT VGGEQRRPPW KRVNSFRVKS SMGVEGQTDM VGKTDSPPVA TTESVFDTGG TEKGKEEQHE VGEDAGELQL QQGKRPLMGA KNAPPTAPCP SHSRKSPVLF PIAERVGALT GSVMHDIIHA EMSFQGPPKI TLQPRPQLTP PQRPNASREW NVTLASDVFS MSPGTVASRA SSLRTGSNGS PNPTPSIPCI LPSTWRSCPV HSVGGSHETA KRPCALLQQN SLKGGIEVLP ASSLCSPVVL KLQPLASAGT SGESSGITSH YVRSQNSRSL SVPMPRKVSL ASTGDPVEKS DVPNFFSHES VTSMNKSSTC STARSGANSN VSVKNLNGTS NFPQPTQNLS FPLLLCSKTN GLRNSENSTR SSRNDCVYHT QARSVGRQGS SPPNDLEKTN PQEKNPSTTS LRQTGASHFP SPSSQQQGRK MRSIQELLVQ EVVSQLRLS
NT Sequence
atgagacagg gaatggtcga aggagaaatg caggaatcgg aggtccccca tgcctcccac gaggcaggag aagaagagag tagtgccacg gtcggcggag aacagcggcg acctccgtgg aagagggtaa attcctttcg ggtgaagtca tccatgggcg ttgaaggtca aaccgacatg gtgggcaaaa cagattctcc tcctgttgcc actacggagt ctgtattcga tacaggtgga acggagaagg ggaaagagga acaacacgaa gttggggagg acgctggaga attgcaacta caacagggaa agagacctct catgggagca aaaaatgctc cccccacggc gccgtgtcca tctcattcaa gaaaatcgcc tgtattattt cctattgctg aacgtgttgg ggccctcact ggctctgtta tgcatgatat cattcatgcc gagatgagtt ttcagggccc accgaaaata acgctgcagc cacgccccca actgacacca ccacaaaggc caaatgcttc tcgagaatgg aatgtaactt tggcctctga tgtattttcc atgtctccag gcactgtcgc ttctcgtgct tcttctttga ggactggctc gaatgggtcg cctaatccta cgccatcgat tccatgcatt ctgccaagca cgtggagaag ttgtccagtg catagcgtgg gcgggtccca tgagacagca aagcgtccat gtgccttgct tcagcagaat agtcttaaag gaggaattga agtcttgccc gcgtcctcct tgtgctctcc ggtagtgctg aagctgcagc ctcttgcgag tgcgggaacc agcggtgagt catccggtat aactagtcat tatgtgcgtt cacagaattc gagaagcttg tctgttccta tgccgagaaa agtttccctt gctagcacgg gggacccagt tgagaagagt gatgtgccta actttttttc acacgaatcc gtcacttcca tgaataaaag cagcacctgt agtacagccc gtagcggtgc caatagtaac gttagcgtga agaacttgaa tggcacctca aattttccac aaccgacgca aaatttgtcg tttcctttat tattgtgctc aaagacaaat ggtttacgaa attcagagaa ttccacgcga agttcccgga atgactgtgt ttatcatacg caggcacgtt ctgtgggacg gcagggttct tcacccccaa atgatttaga aaagactaat ccacaggaga aaaatccttc caccacctcc ctgaggcaaa caggcgccag tcatttcccc agtccgtcaa gtcaacaaca gggaaggaag atgcgtagta ttcaggagtt actagtgcaa gaagttgtta gccagctgcg tctctcgtag
Details for TrcrA.19097.a.B1.GE39112
HARVESTED ON: data unavailable
SEQUENCED ON: 3/27/2015
EXPECTED MW: 51kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 95
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMR QGMVEGEMQE SVVPHASHEA EEEESSAMVG GEQRRPPWKR VNSFRVKSSM GVEGPTDMVG KTDSPPVATT ESVFDTGGTE KGKEEQREVG GDAGELQLQQ GQRLLTGAQN APPTVPCQSH SRKSPVLFPI AERVGALTGS VMHDIIHAEM SFQGPPKLTL QPRPQLTPPQ RPNASREWNV TLASDVFSMS PGTVASRASS LRTGSNGSPN PTPSIPCILP NTWRSCPVHS VGGSHETAKR PCALLQQNCL KGGIEVLPAS SLCSPVVLRL QPLASAGNSG GSSGITSHYV RSQNSRCLSV PMPRKVSLAS TGDPVEKSDV PNFFSHESVT SMNKSSTCST ARSGANSNVS VKNLNGTSNF PQPTQNLSFP LLLCSKTNGL RNSENSTRSS RNDCVYRTQA RSVGRQGSSP PNDLEKTNPQ EKSPSTTSLR QTGGSHFPSP SRQQQGRKMR SIQELLVQE
Validated NT Sequence
aacttcttgc actagtaact cctgaatact acgcatcttc cttccctgtt gttgacgtga cggactgggg aaatgactgc cgcctgtttg cctcagggag gtggtggaag gacttttctc ttgtggatta gtcttttcta aatcatttgg gggtgaagaa ccctgccgtc ccacagaacg tgcctgcgta cgataaacac agtcattccg ggaacttcgc gtggaattct ctgaatttcg taaaccgttt gtctttgagc acaataataa aggaaacgac aaattttgcg tcggttgtgg aaaatttgag gtgccattca agttcttcac gctaacgtta ctattggcac cgctacgggc tgtactacag gtgctgcttt tattcatgga agtgacggat tcgtgtgaaa aaaagttagg cacatcactc ttctcaactg ggtcccccgt gctagcaagg gaaacttttc tcggcatagg aacagacaag catctcgaat tctgtgaacg cacataatgg ctagttatac cggatgaccc accgctgttt cccgcactcg caagaggctg cagcctcagc actaccggag agcacaagga ggacgcgggc aagacttcaa ttcctccttt aagacaattc tgctgaagca aggcacatgg acgctttgct gtctcatggg acccgcccac gctatgcact ggacaacttc tccacgtgtt tggcagaatg catggaatcg atggcgtagg attaggcgat ccattcgagc cagtcctcaa agaagaagca cgagaagcga cagtgcctgg agacatggaa aatacatcag aggccaaagt tacattccat tctcgagaag catttggcct ttgtggtggt gtcagttgag gacgtggctg cagcgttagt ttcggtgggc cctgaaagct catctcggca tgaatgatat catgcataac agagccagtg agggccccaa cacgttcagc aataggaaat aatacaggcg attttcttga atgagattga cacggcaccg tggggggagc attttgtgct cccgtgagaa gtctctgtcc ctgttgtagt tgcaattctc cagcgtcccc cccaacttcg cgttgttcct ctttcccctt ctccgttcca cctgtatcga atacagactc cgtagtggca acaggaggag aatctgtttt gcccaccatg tcggttggac cttcaacgcc catggatgac ttcacccgaa aggaatttac cctcttccat ggaggtcgcc gctgttctcc gccgaccatg gcactactct cttcttcttc tgcctcgtgg gaggcatggg ggaccaccga ttcctgcatt tctccttcga ccattccctg tctcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMR QGMVEGEMQE SEVPHASHEA GEEESSATVG GEQRRPPWKR VNSFRVKSSM GVEGQTDMVG KTDSPPVATT ESVFDTGGTE KGKEEQHEVG EDAGELQLQQ GKRPLMGAKN APPTAPCPSH SRKSPVLFPI AERVGALTGS VMHDIIHAEM SFQGPPKITL QPRPQLTPPQ RPNASREWNV TLASDVFSMS PGTVASRASS LRTGSNGSPN PTPSIPCILP STWRSCPVHS VGGSHETAKR PCALLQQNSL KGGIEVLPAS SLCSPVVLKL QPLASAGTSG ESSGITSHYV RSQNSRSLSV PMPRKVSLAS TGDPVEKSDV PNFFSHESVT SMNKSSTCST ARSGANSNVS VKNLNGTSNF PQPTQNLSFP LLLCSKTNGL RNSENSTRSS RNDCVYHTQA RSVGRQGSSP PNDLEKTNPQ EKNPSTTSLR QTGASHFPSP SSQQQGRKMR SIQELLVQEV VSQLRLS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagac agggaatggt cgaaggagaa atgcaggaat cggaggtccc ccatgcctcc cacgaggcag gagaagaaga gagtagtgcc acggtcggcg gagaacagcg gcgacctccg tggaagaggg taaattcctt tcgggtgaag tcatccatgg gcgttgaagg tcaaaccgac atggtgggca aaacagattc tcctcctgtt gccactacgg agtctgtatt cgatacaggt ggaacggaga aggggaaaga ggaacaacac gaagttgggg aggacgctgg agaattgcaa ctacaacagg gaaagagacc tctcatggga gcaaaaaatg ctccccccac ggcgccgtgt ccatctcatt caagaaaatc gcctgtatta tttcctattg ctgaacgtgt tggggccctc actggctctg ttatgcatga tatcattcat gccgagatga gttttcaggg cccaccgaaa ataacgctgc agccacgccc ccaactgaca ccaccacaaa ggccaaatgc ttctcgagaa tggaatgtaa ctttggcctc tgatgtattt tccatgtctc caggcactgt cgcttctcgt gcttcttctt tgaggactgg ctcgaatggg tcgcctaatc ctacgccatc gattccatgc attctgccaa gcacgtggag aagttgtcca gtgcatagcg tgggcgggtc ccatgagaca gcaaagcgtc catgtgcctt gcttcagcag aatagtctta aaggaggaat tgaagtcttg cccgcgtcct ccttgtgctc tccggtagtg ctgaagctgc agcctcttgc gagtgcggga accagcggtg agtcatccgg tataactagt cattatgtgc gttcacagaa ttcgagaagc ttgtctgttc ctatgccgag aaaagtttcc cttgctagca cgggggaccc agttgagaag agtgatgtgc ctaacttttt ttcacacgaa tccgtcactt ccatgaataa aagcagcacc tgtagtacag cccgtagcgg tgccaatagt aacgttagcg tgaagaactt gaatggcacc tcaaattttc cacaaccgac gcaaaatttg tcgtttcctt tattattgtg ctcaaagaca aatggtttac gaaattcaga gaattccacg cgaagttccc ggaatgactg tgtttatcat acgcaggcac gttctgtggg acggcagggt tcttcacccc caaatgattt agaaaagact aatccacagg agaaaaatcc ttccaccacc tccctgaggc aaacaggcgc cagtcatttc cccagtccgt caagtcaaca acagggaagg aagatgcgta gtattcagga gttactagtg caagaagttg ttagccagct gcgtctctcg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat