TrcrA.19092.a

Uncharacterized protein

CENTER ID: TrcrA.19092.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.19092.a.B1.GE39111 full length 1 416
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:TCSYLVIO_007527
UniProt: K4DSX4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPKCGAPRRP SMAGERRTAG RLSAQERWLQ SFNPYCQPLL LRRKPSSLLS SAAVPGRRAR SAASPRHKAV EIRPEVGLPV TNGDALLQLT QEEWSICNLM VTVLEHKSTE EAQRIISEVA QLALSRRLLT RYSGVYADGH VNGEEDTRGD EEEDGRAQLI RHLQEHQRRE AERRKQLCDS NRQSQQGDIR ARCRKLFNRS GVNGDGKALK AAKKRCLPRR CKSSAEGNKM QQQGGRVNLE EMVMTKTTVP VPSVPLNVPR RAKASKGVLS FDECCADRYG GGAYSNESMA SVLPLRCAAV SPTDDSVRRS NSFPSAGVNY DDHDATKGIF LHDDSNDIHT NGAPSALIST MRPATASETF NDLPSLSGSA LPPSHLLDGH NGAGLPPLKP TTPTAGSKTS AQLRAKVTSS SVSLLL
NT Sequence
atgccaaagt gcggggcgcc aaggcggcca tcgatggcgg gtgagcgacg caccgctgga cgtctttcag cgcaagagag atggctgcag tcgttcaacc catactgtca accgctgttg ctgcgacgga agccatcatc attgttgtcg tctgccgcgg tacccggaag gcgtgctcgt tccgcagcat cgccccgcca caaggcagtg gagatccgac cagaggtggg gctccccgtg acgaacggtg atgcgcttct ccagctcacg caggaggagt ggagtatatg caacctgatg gtcactgtgc tcgagcacaa atccacggag gaagctcaac gcattatttc cgaggtggcc caactggcgc tgagccgccg tctgctaacc cgatacagtg gcgtgtacgc cgatggccat gtcaatggcg aggaagacac tcgaggagac gaggaagaag acgggcgagc tcaactgatt cgtcatctac aggaacatca gcgacgcgag gcggagagac gaaagcagtt gtgtgattcc aaccgccaga gtcaacaggg ggatatacga gcacgttgca ggaaactttt taaccgcagt ggtgtaaatg gcgacggtaa ggctttgaag gcggcgaaga agagatgttt gccacgcagg tgtaagtcat ctgccgaggg aaataagatg cagcaacagg gtggacgtgt caatttggag gaaatggtga tgacaaagac aacggttcct gttccgagcg tgccattgaa tgttccacgg cgggcaaagg catccaaggg cgtactatcc tttgatgaat gctgtgcaga ccggtacggt ggtggtgcgt attcaaatga aagtatggca tccgtcttgc ctcttcgctg cgctgcagtg agcccaaccg atgactccgt tcggcgatca aattcatttc cctcggcagg tgttaattac gatgatcatg atgccactaa gggcattttt ttacatgatg atagcaatga tattcatacc aatggagcgc cttcggcgct catctccacc atgcgtcctg caacggccag cgagacgttc aatgatttgc catccctctc agggtccgca ttgccgccgt cacacttgtt ggatgggcac aacggcgctg gacttccacc gttgaagccg acgaccccta ctgctggatc gaagacatcg gcacaactga gggcaaaagt gacttcctct tccgtgtctc ttcttctttg a
Details for TrcrA.19092.a.B1.GE39111
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 46kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 93
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMP KCGAPRRPSA AGERRTAGHL SAQERWLQSF NPYCQPLLLR RKTSSFLSSA AVPGRRARSA ASPRHKAVEV RPEVGLPVTN GDALFQLTQE EWSICNLMVT VLEHKSTEEA QRIISEVAQL ALSRRLLARY SGVYADGHVN GEKDTRGDEE EDGRAQLIRH LQEHQRREAE RRRQLCDSNR QSQQGDIRAR CRKLFNRGGV NGDGRALTAA KKRCLPRRCK SSADGNTMQQ QGGHVNVEEM VMTKTTVPVP SVPLNVPRRA KASKGVLSFD ECCADRYGGG AYSNDSMASV LPLRCAAMSP IDDSVRRSNS FPSAGVKYDD HDAIKGIFSH DDSNEIHTNG APSAFISTMR PATTSETFND LSSLSGSALT PSHLLDGHNG AGLPPLKPTA PNAGSKTSAQ LRAKVT
Validated NT Sequence
ggaagtcact tttgccctca gttgtgccga tgtcttcgat ccagcattag gggcagtcgg cttcaacggt ggaagtccag cgccgttgtg cccatccaac aagtgtgacg gcgtcaatgc ggaccctgag agggatgaca aatcattgaa cgtctcgctg gtcgttgcag gacgcatggt ggagatgaac gccgaaggcg ctccattggt atgaatttca ttgctatcat catgtgaaaa aatgccctta atggcatcat gatcatcgta cttaacacct gccgagggaa atgaatttga ccgccgaacg gagtcatcaa ttgggctcat tgcagcgcag cgaagaggca agacggatgc catactgtca tttgaatacg caccaccacc gtaccggtct gcacagcatt catcaaagga gagtacgccc ttggatgcct ttgcccgccg tggaacattc aatggcacgc tcggaacagg aaccgttgtc tttgtcatca ccatttcctc cacattgaca tgtccaccct gttgctgcat cgtatttccg tcggcagatg acttacacct gcgtggcaaa catctcttct tcgccgccgt caaagctcta ccgtcgccat ttacaccacc gcggttaaaa agtttcctgc aacgtgctcg tatatccccc tgttgactct ggcggttgga atcacacaac tgccttcgtc tctccgcctc gcgtcgctga tgttcctgta gatgacgaat cagttgagct cgcccgtctt cttcctcgtc tcctcgagtg tctttctcgc cattgacatg gccatcggcg tacacgccac tgtatcgggc tagcagacgg cggctcagcg ccagttgggc cacctcggaa ataatgcgtt gagcttcctc cgtggatttg tgctcgagca cagtgaccat caggttgcat atactccact cctcctgcgt gagctggaaa agcgcatcac cgttcgtcac ggggagcccc acctctggtc ggacctccac tgccttgtgg cggggcgatg ctgcggaacg agcacgcctt ccgggtaccg cggcagacga caaaaatgat gatgtcttcc gtcgcagcaa cagcggttga cagtatgggt tgaacgactg cagccatctc tcttgcgctg aaagatgtcc agcggtgcgt cgctcacccg ccgccgatgg ccgccttggc gccccgcact ttggcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMP KCGAPRRPSM AGERRTAGRL SAQERWLQSF NPYCQPLLLR RKPSSLLSSA AVPGRRARSA ASPRHKAVEI RPEVGLPVTN GDALLQLTQE EWSICNLMVT VLEHKSTEEA QRIISEVAQL ALSRRLLTRY SGVYADGHVN GEEDTRGDEE EDGRAQLIRH LQEHQRREAE RRKQLCDSNR QSQQGDIRAR CRKLFNRSGV NGDGKALKAA KKRCLPRRCK SSAEGNKMQQ QGGRVNLEEM VMTKTTVPVP SVPLNVPRRA KASKGVLSFD ECCADRYGGG AYSNESMASV LPLRCAAVSP TDDSVRRSNS FPSAGVNYDD HDATKGIFLH DDSNDIHTNG APSALISTMR PATASETFND LPSLSGSALP PSHLLDGHNG AGLPPLKPTT PTAGSKTSAQ LRAKVTSSSV SLLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccaa agtgcggggc gccaaggcgg ccatcgatgg cgggtgagcg acgcaccgct ggacgtcttt cagcgcaaga gagatggctg cagtcgttca acccatactg tcaaccgctg ttgctgcgac ggaagccatc atcattgttg tcgtctgccg cggtacccgg aaggcgtgct cgttccgcag catcgccccg ccacaaggca gtggagatcc gaccagaggt ggggctcccc gtgacgaacg gtgatgcgct tctccagctc acgcaggagg agtggagtat atgcaacctg atggtcactg tgctcgagca caaatccacg gaggaagctc aacgcattat ttccgaggtg gcccaactgg cgctgagccg ccgtctgcta acccgataca gtggcgtgta cgccgatggc catgtcaatg gcgaggaaga cactcgagga gacgaggaag aagacgggcg agctcaactg attcgtcatc tacaggaaca tcagcgacgc gaggcggaga gacgaaagca gttgtgtgat tccaaccgcc agagtcaaca gggggatata cgagcacgtt gcaggaaact ttttaaccgc agtggtgtaa atggcgacgg taaggctttg aaggcggcga agaagagatg tttgccacgc aggtgtaagt catctgccga gggaaataag atgcagcaac agggtggacg tgtcaatttg gaggaaatgg tgatgacaaa gacaacggtt cctgttccga gcgtgccatt gaatgttcca cggcgggcaa aggcatccaa gggcgtacta tcctttgatg aatgctgtgc agaccggtac ggtggtggtg cgtattcaaa tgaaagtatg gcatccgtct tgcctcttcg ctgcgctgca gtgagcccaa ccgatgactc cgttcggcga tcaaattcat ttccctcggc aggtgttaat tacgatgatc atgatgccac taagggcatt tttttacatg atgatagcaa tgatattcat accaatggag cgccttcggc gctcatctcc accatgcgtc ctgcaacggc cagcgagacg ttcaatgatt tgccatccct ctcagggtcc gcattgccgc cgtcacactt gttggatggg cacaacggcg ctggacttcc accgttgaag ccgacgaccc ctactgctgg atcgaagaca tcggcacaac tgagggcaaa agtgacttcc tcttccgtgt ctcttcttct ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat