TrcrA.18364.a

phosphatidylinositol-4-phosphate 5-kinase-like protein

CENTER ID: TrcrA.18364.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18364.a.B1.GE35425 full length 1 268
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053509707.29
RefSeq: XP_805019.1
UniProt: Q4CSB4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
AYVTLGAIYT GKTVIFDTFV PLAINTLCIH SQRFPVDKYA LTYKVGALAI GACGAIDCET GSTVTEIRRD AYGFTVPIGV TFVETTGADI LSTVERLRKR KPRDLRCHIW IQIQCVILHR VRQTSTVGRL TLVDLCGPGP LPTQKEDVAS ARFANRSFGH IVAVLESLQD SQGVVQYNKS LETALLSDVF GGNALTAIFG TLSSAVESVS ESRQTLRALT MASKVVNRPI LPRFVSSDEL RWREVVAATS SAEVASPCLL EVDELRDM
NT Sequence
gcctacgtta ctctaggtgc gatttacact ggcaagacag tcatttttga tacatttgtt ccattagcca taaacaccct ttgtattcac tctcaacgat tccccgtgga caagtacgcc ttgacctaca aggtgggtgc tttggcgatt ggtgcctgtg gggcgattga ctgtgagacg ggctccaccg tgactgagat tcgacgcgat gcctacggtt tcactgttcc tataggtgta acatttgtcg agacgactgg cgccgatatt ctttccaccg tggaaaggtt aagaaaacga aagccgcgtg atcttcgttg tcacatatgg attcagattc agtgtgtgat tcttcacaga gtgcgacaga cgtccacagt gggacgtctg acactcgtgg atttgtgtgg tcccgggccc cttcctacac agaaggagga tgtggccagt gctcggtttg cgaaccgttc ttttggacat attgtcgcgg tgctggaatc tctccaggat tcacaaggtg tagtgcagta caacaaatcc ctggagactg ctctactctc tgatgtcttt ggtgggaatg cactgaccgc catttttggt accctttctt ctgcagtgga aagcgttagc gagagccgcc agacgttgcg ggccctgacg atggcgtcga aggtggtgaa ccgtcccata ttgccgcgct ttgtcagcag cgacgaactc cggtggcggg aagtagtcgc tgcaacaagt tctgcagagg tggcgtcgcc atgtctattg gaagtagatg aattgcgaga catgtag
Details for TrcrA.18364.a.B1.GE35425
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA YVTLGAIYTG KTVIFDTFVP LAINTLCNHS QRFPVDKYAL TYKVGALAIG ACEAIDCETG STVTEIRRDA YGFTVPIGVT FVETTGADIL STVERLRKRK PRDLRCHIWI QIQCVILHRV RQTSTVGRLT LVDLCGPGPL PTQKEAVASA RFANRSFGHI VAVLESLQDS QGVVQYNKSL ETALLSDVLG GNALTTIFGT LSSAVESVSE SRQTLRALTM ASXVVNRPIL PRFVSSDELR WREVVAATSS AEVASPCLLX SR
Validated NT Sequence
anattttgtt tactttaaga aggagatata ccatggctca ccaccaccac caccatatgg cctacgttac tctaggtgcg atttacactg gcaagacagt catttttgat acatttgttc cattagccat aaacaccctt tgtaatcact ctcaacgatt ccccgtggac aagtacgctt tgacatacaa ggtgggtgct ttggcgattg gtgcctgtga ggcgattgac tgtgagacgg gctccaccgt gactgagatt cgacgcgatg cctacggttt cactgttcct ataggtgtaa catttgtcga gacgactggc gccgatattc tttccaccgt ggaaaggtta agaaaacgaa agccgcgtga tcttcgttgt cacatatgga ttcagattca gtgtgtgatt cttcacagag tgcgacagac gtccacagtg ggacgtctga cactcgtcga tttgtgtggt cccgggcccc ttcctacaca gaaggaggct gtggccagtg ctcggtttgc gaaccgttct tttggacata ttgtcgcggt gctggaatct ctccaggact cacaaggtgt agtgcagtac aacaaatccc tggagactgc tctactctct gatgtccttg gtgggaatgc actgactacc atttttggta ccctttcttc tgcagtggaa agcgttagcg agagccgcca gacgttgcgg gccctgacca tggcgtcgaa ngtggtgaac cgtcctatat tgccgcgctt tgtcagcagc gacgaactcc ggtggcggga agtagttgct gcaacaagtt ctgcagaggt ggcgtcgcca tgtctattgg naagtagatg aattgcgaga catgtaagtg agtaagatag gatccggctg ctannnnccc cc
Expected Protein Sequence
MAHHHHHHMA YVTLGAIYTG KTVIFDTFVP LAINTLCIHS QRFPVDKYAL TYKVGALAIG ACGAIDCETG STVTEIRRDA YGFTVPIGVT FVETTGADIL STVERLRKRK PRDLRCHIWI QIQCVILHRV RQTSTVGRLT LVDLCGPGPL PTQKEDVASA RFANRSFGHI VAVLESLQDS QGVVQYNKSL ETALLSDVFG GNALTAIFGT LSSAVESVSE SRQTLRALTM ASKVVNRPIL PRFVSSDELR WREVVAATSS AEVASPCLLE VDELRDM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcct acgttactct aggtgcgatt tacactggca agacagtcat ttttgataca tttgttccat tagccataaa caccctttgt attcactctc aacgattccc cgtggacaag tacgccttga cctacaaggt gggtgctttg gcgattggtg cctgtggggc gattgactgt gagacgggct ccaccgtgac tgagattcga cgcgatgcct acggtttcac tgttcctata ggtgtaacat ttgtcgagac gactggcgcc gatattcttt ccaccgtgga aaggttaaga aaacgaaagc cgcgtgatct tcgttgtcac atatggattc agattcagtg tgtgattctt cacagagtgc gacagacgtc cacagtggga cgtctgacac tcgtggattt gtgtggtccc gggccccttc ctacacagaa ggaggatgtg gccagtgctc ggtttgcgaa ccgttctttt ggacatattg tcgcggtgct ggaatctctc caggattcac aaggtgtagt gcagtacaac aaatccctgg agactgctct actctctgat gtctttggtg ggaatgcact gaccgccatt tttggtaccc tttcttctgc agtggaaagc gttagcgaga gccgccagac gttgcgggcc ctgacgatgg cgtcgaaggt ggtgaaccgt cccatattgc cgcgctttgt cagcagcgac gaactccggt ggcgggaagt agtcgctgca acaagttctg cagaggtggc gtcgccatgt ctattggaag tagatgaatt gcgagacatg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat