SSGCID: Target Page

TrcrA.18360.a

hypothetical protein, conserved

CENTER ID:	TrcrA.18360.a
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.18360.a.B1.GE35424	full length		1	603

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053511761.10
RefSeq:	XP_810648.1
UniProt:	Q4D8F3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKMVPDGGEP TCEELDALRE RLSSPELDQR HLFNGWPASA EEYTEEQRRL MLELFRFRDH YSGGVEQYVR NAQRLFKGLK NTRHEYAALE LPPYVYEAPS LFDRSEELMN LEREGLGYVK KSVFVLVAGG LGERLGYSGI KIGLPVETAT NRCYLEHYLR WIKHIAGPNA PFVIMTSDNT HERTEKLLRG LGLNMTNVHL LKQETVFCFN DITAHLAIEN GKLLRKPHGH GDVHSLLYRS VDRSSGKRLV ELWQSQGYSY IVFLQDTNAT ATLTIPVSLA ISAKHRLAMN FTCIPRQPKE AIGLLCKVRM FGSNIERTIN VEYDIFESLA ASLTELGGDR AAPGSIYSYF PGSINTLILN MDDYIPLLTE FCGVVPEFIN PKYTDDSKTT FKPCRIESLM QDIALLFGPE EHRVGALRFS RFTYQPVKNG LQDGIKKFAQ GLAAYCAATG EEGFYEAIRL RLQAAGLNLP TRPKDAYDVD FGAGLKVRLF PIIVADAMAM GVSVEDITQR LLPHPEHVKV SARSVLLVEG CVRIESLDLD GALRLVGPTD ENAAPLVINA MTVKNAGWVV RPLSADESAD EIYRIRGYVI EEKEMQAVHH AKL

NT Sequence

atgaagatgg tgcctgacgg aggagaaccg acgtgcgagg agctcgacgc actgcgcgag cggctcagca gtccagaatt ggaccagcgc cacctgttta atggttggcc tgcgtcggcg gaggaataca cggaggagca gagacggttg atgctggaat tatttcgctt cagagaccat tacagtggtg gcgttgaaca gtacgtgcgt aatgcccagc gtcttttcaa ggggttgaaa aacacacgac acgagtacgc cgctcttgaa ctcccgcctt acgtgtacga ggcgccttct ctctttgacc gcagcgagga gttgatgaat ctcgagcgtg aaggattggg ttacgtcaag aagagtgttt ttgtgctggt agcggggggt ctgggggagc gtctcgggta ctctgggatt aaaattggtc tgcctgtcga gacggctacg aaccgttgct accttgagca ctatcttcgg tggatcaaac acatcgcagg accgaatgcc ccctttgtca ttatgacatc cgataatacc cacgaacgca cggaaaagct tcttcgaggc ttggggctta atatgacaaa tgtgcatctt ttgaagcaag agacagtctt ttgttttaac gatatcacag cacaccttgc cattgaaaat gggaagttac ttcggaaacc ccacggccac ggagatgtac actcattgct gtaccgatct gttgatcgct cgtccgggaa gcggcttgtg gagttatggc agtctcaggg ttattcttac attgtttttc tacaagatac aaacgctact gccacattga cgattccggt tagtctggcg ataagtgcca aacatcgcct ggcaatgaat ttcacgtgca ttcctcgtca acccaaggag gcgattggac tgctgtgtaa ggttcgcatg tttggttcaa atattgagcg gacaataaac gttgagtacg acatttttga gtctctggcg gcatcattga cagaactcgg gggtgaccgg gcagctcccg ggtccattta ttcctatttc cctggaagca ttaatacgct tattctgaac atggatgatt acatcccgct gcttaccgaa ttttgtggcg ttgttccgga gtttattaac cccaaatata cagacgacag caagacaaca ttcaaaccat gtcgtattga gtcacttatg caggacattg cgcttctttt tggccctgag gaacatcgcg ttggggcttt gcgatttagt cgttttacat atcaaccggt gaagaatggg ctacaagacg ggatcaagaa gtttgctcag ggacttgcgg cgtactgcgc agccacaggg gaggagggct tctatgaggc cattcgtctt cgtttgcagg ctgccggact gaatttgcca acacgcccca aagatgccta cgatgtagat tttggggcgg ggctgaaagt gcggctgttc cccatcattg ttgcggacgc catggcgatg ggggtttccg ttgaagacat cacacaacga cttctcccgc accctgaaca cgttaaagtg agcgcacgga gtgttctgct tgttgagggt tgcgtacgca ttgagagcct cgaccttgat ggtgctctgc gtcttgtggg cccgacagac gaaaatgcag cgccgcttgt tattaatgcg atgaccgtga aaaatgccgg ctgggtggta cgcccgttat ccgcggatga gagtgcagat gagatttatc gaatacgggg atatgtgatt gaggaaaaag aaatgcaggc agttcatcat gcgaagcttt ag

Details for TrcrA.18360.a.B1.GE35424

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	68kDa
OBSERVED MW:	68kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	82
PERCENT COVERAGE:	64

Validated AA Sequence

MAHHHHHHMK MVPDGGEPTC EELDALRERL SSPELDQCHL FNGWPASAEE YTEEQRRLML ELFRFRDHYS GGVEQYVRNA QRLFKGLKNT RHEYAALELP SYVYEAPSLF DRSEELMSLE REGLSYVKKS VFVLVAGGLG ERLGYSGIKI GLPVETATNR CYLEHYLRWI KHIAGPNAPF VIMTSDNTHE RTEKLLRGLG LNMTNVHLLK QETVFCFNDI TAHLAFENRK LLRKPHGHGD VHTLLYRSVD RSSGKRLVEL WQSXGYSYIV FLQDTNATAT LTIPVSLAIS XXHRLAMNFT CIPRQPXXAI GLLCXVRMFG SNIERTINVX YDIFEXLXXS XXEXXGXXGX SXXXXXXXXX XXXXXXXXXX AXXXXXXXXX XXXXXXXXXX XXXX

Validated NT Sequence

attttgtnnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatgaag atggtgcctg acggaggaga accgacgtgc gaggagctcg acgccctgcg cgagcggctc agcagcccag aattggacca gtgccacctg tttaatggtt ggcctgcgtc ggcggaggaa tacacggagg agcagcgacg gttgatgctg gaattatttc gcttcagaga ccattacagt ggtggcgttg aacagtacgt gcgtaatgcc cagcgtcttt tcaaggggtt gaaaaataca cgacacgagt acgccgctct tgaactcccg tcttacgtgt acgaggcgcc ttctctcttt gaccgcagcg aggagttgat gagtctcgag cgtgaaggat tgagttacgt caagaagagt gtttttgtgc tggtagcggg gggtctgggg gagcgtctcg ggtactctgg gattaaaatt ggtctgcctg tcgagacggc tacgaaccgt tgctaccttg agcactatct tcggtggatc aaacacatcg caggaccgaa tgcccccttt gtcattatga catccgataa tacccacgaa cgcacggaaa agcttcttcg aggcttgggg cttaatatga caaatgtgca tcttttgaag caagagacag ttttttgttt taacgatatc acagcacacc ttgcctttga aaataggaag ttgcttcgga aaccccacgg ccacggagat gtacacacat tgctgtaccg atctgttgat cgctcgtccg ggaagcggct tgtggagtta tggcagtctc anggttattc ttacattgtt tttctacaag atacaaacgc tactgccaca ttgacgattc cggttagtct ggcgataagt gncnaacatc gcctggcaat gaatttcacg tgcattcctc gtcaacccnn ggangcgatt ggnctgctgt gtanggttcg catgtttggn tcaaatattg agcggacaat aaacgttgan tacgacattt ttgagtnnct ggnngnatca tnganngaan ncngggggng annnggnnnc tcnnnnnntt nntnnntnnc ntgnnncntn nnncnctntn tgannangnn gatncntncn gcnncnnncn nnnntnnnnn nnnnnnnnnt ngannnnncn nannnnnnnn annnnnnnnn nnnnnnnnnn nnnt

Expected Protein Sequence

MAHHHHHHMK MVPDGGEPTC EELDALRERL SSPELDQRHL FNGWPASAEE YTEEQRRLML ELFRFRDHYS GGVEQYVRNA QRLFKGLKNT RHEYAALELP PYVYEAPSLF DRSEELMNLE REGLGYVKKS VFVLVAGGLG ERLGYSGIKI GLPVETATNR CYLEHYLRWI KHIAGPNAPF VIMTSDNTHE RTEKLLRGLG LNMTNVHLLK QETVFCFNDI TAHLAIENGK LLRKPHGHGD VHSLLYRSVD RSSGKRLVEL WQSQGYSYIV FLQDTNATAT LTIPVSLAIS AKHRLAMNFT CIPRQPKEAI GLLCKVRMFG SNIERTINVE YDIFESLAAS LTELGGDRAA PGSIYSYFPG SINTLILNMD DYIPLLTEFC GVVPEFINPK YTDDSKTTFK PCRIESLMQD IALLFGPEEH RVGALRFSRF TYQPVKNGLQ DGIKKFAQGL AAYCAATGEE GFYEAIRLRL QAAGLNLPTR PKDAYDVDFG AGLKVRLFPI IVADAMAMGV SVEDITQRLL PHPEHVKVSA RSVLLVEGCV RIESLDLDGA LRLVGPTDEN AAPLVINAMT VKNAGWVVRP LSADESADEI YRIRGYVIEE KEMQAVHHAK L

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga tggtgcctga cggaggagaa ccgacgtgcg aggagctcga cgcactgcgc gagcggctca gcagtccaga attggaccag cgccacctgt ttaatggttg gcctgcgtcg gcggaggaat acacggagga gcagagacgg ttgatgctgg aattatttcg cttcagagac cattacagtg gtggcgttga acagtacgtg cgtaatgccc agcgtctttt caaggggttg aaaaacacac gacacgagta cgccgctctt gaactcccgc cttacgtgta cgaggcgcct tctctctttg accgcagcga ggagttgatg aatctcgagc gtgaaggatt gggttacgtc aagaagagtg tttttgtgct ggtagcgggg ggtctggggg agcgtctcgg gtactctggg attaaaattg gtctgcctgt cgagacggct acgaaccgtt gctaccttga gcactatctt cggtggatca aacacatcgc aggaccgaat gccccctttg tcattatgac atccgataat acccacgaac gcacggaaaa gcttcttcga ggcttggggc ttaatatgac aaatgtgcat cttttgaagc aagagacagt cttttgtttt aacgatatca cagcacacct tgccattgaa aatgggaagt tacttcggaa accccacggc cacggagatg tacactcatt gctgtaccga tctgttgatc gctcgtccgg gaagcggctt gtggagttat ggcagtctca gggttattct tacattgttt ttctacaaga tacaaacgct actgccacat tgacgattcc ggttagtctg gcgataagtg ccaaacatcg cctggcaatg aatttcacgt gcattcctcg tcaacccaag gaggcgattg gactgctgtg taaggttcgc atgtttggtt caaatattga gcggacaata aacgttgagt acgacatttt tgagtctctg gcggcatcat tgacagaact cgggggtgac cgggcagctc ccgggtccat ttattcctat ttccctggaa gcattaatac gcttattctg aacatggatg attacatccc gctgcttacc gaattttgtg gcgttgttcc ggagtttatt aaccccaaat atacagacga cagcaagaca acattcaaac catgtcgtat tgagtcactt atgcaggaca ttgcgcttct ttttggccct gaggaacatc gcgttggggc tttgcgattt agtcgtttta catatcaacc ggtgaagaat gggctacaag acgggatcaa gaagtttgct cagggacttg cggcgtactg cgcagccaca ggggaggagg gcttctatga ggccattcgt cttcgtttgc aggctgccgg actgaatttg ccaacacgcc ccaaagatgc ctacgatgta gattttgggg cggggctgaa agtgcggctg ttccccatca ttgttgcgga cgccatggcg atgggggttt ccgttgaaga catcacacaa cgacttctcc cgcaccctga acacgttaaa gtgagcgcac ggagtgttct gcttgttgag ggttgcgtac gcattgagag cctcgacctt gatggtgctc tgcgtcttgt gggcccgaca gacgaaaatg cagcgccgct tgttattaat gcgatgaccg tgaaaaatgc cggctgggtg gtacgcccgt tatccgcgga tgagagtgca gatgagattt atcgaatacg gggatatgtg attgaggaaa aagaaatgca ggcagttcat catgcgaagc tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat