SSGCID: Target Page

TrcrA.18346.a

dual-specificity protein phosphatase, putative

CENTER ID:	TrcrA.18346.a
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.18346.a.B1.GE35416	full length		1	375

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.18346.a.B1.PS01602	full length		1	375

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053509779.60
RefSeq:	XP_808688.1
UniProt:	Q4D2T0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

HGSSSSLLSS CTTVLPYSSL GKISSFSTCG REPPTKILDF LYIGGVRDAT NAEFLRRENI VTILNVSREE YWSVDRSIVI HPFAVDDTTE ANIQQFFRPT HVILEQVRKA YYDAKQRGAS VCPRALVHCQ RGKSRSVTIV LAYLIYRNGW TVAEALQYVT RRRPRVEPNL GFIDALRAYQ ESMDVEERTR RCSSLGLAVR NLPRDTASSF VHKFFEDHVG CVQEVMMHSS RPNQSRNIAA PNTNGVDQGK RGNDDNDPPA SFGLSGRRDG NSSSGIAVKT CSNDADGNTL CLVFFSAAEC VRLAKLLYMR RPELFNVLGV VDGKELRLTI PSKLIRLSRT AHAHCDDGDN TRSKKSSQGE EDTPKGDAEK NDAGT

NT Sequence

cacggtagct catccagttt gttgagtagt tgcactactg tactacctta ttcttctttg ggaaaaatct cgtcattttc gacgtgtggc cgtgagccac cgacaaaaat tcttgacttt ctttacattg ggggtgtgcg tgatgccacg aatgcggaat ttttgcgtcg agagaatatt gtaactattc ttaatgtatc gcgtgaggaa tattggtctg ttgatcgcag cattgttatt catccatttg cagtggatga caccaccgag gcaaacattc agcagttttt tcgcccaacc cacgtaatcc ttgagcaagt ccgtaaagcg tactatgatg ccaagcaacg tggcgcgagt gtttgtcccc gcgctctggt gcactgccaa cgcgggaaga gccgctccgt caccattgtg ctcgcctatc tgatctaccg caacggctgg acagtagcag aggcgctgca atacgtcaca cgccggcggc ctcgcgtgga accaaatttg ggttttattg acgcgcttcg ggcgtatcag gagtcgatgg acgtggagga gcgaacgcga cgctgcagtt cactcggtct tgccgtgcgt aaccttccac gagatacggc gtcgtcgttt gttcacaagt tctttgagga tcatgttggt tgtgtgcagg aggtgatgat gcactcctcc cgaccgaacc aaagccgtaa catcgccgct cccaatacca acggcgttga ccagggcaag cgcggcaacg acgacaatga ccctcccgcc tccttcgggc tttcaggcag gagggacggc aacagtagca gcggcatcgc tgtcaaaact tgcagcaacg atgccgatgg caatacgttg tgcctcgttt tcttctctgc ggcggagtgt gtgcggctgg ccaaactgtt atacatgcga cggcctgagc tcttcaatgt gctcggtgtc gtggatggta aagagttgcg gctcacgatc cccagcaaac tgataagact gtcgagaaca gcacacgccc attgcgatga tggtgacaac acccgaagca agaagtcttc tcagggggag gaggacacgc cgaagggaga cgcggaaaaa aacgacgcag ggacataa

Details for TrcrA.18346.a.B1.GE35416

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	42kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	50

Validated AA Sequence

MDVEERTRRC SSLGLAVRNL PRDTASSFVH KFFEDHVGCV QEVMMHSSRP NQNRNIAVPN TNGVDQGKRG NDDNDPPASF GLSGRRDGNS SSSISVKTCG NDADGNTLCL VFFSAAECVR LAKLLYMRRP ELFNVLGVVD GKELRLTIPS KLIRLSRTAH AHCDDGDNTR SKKSSQGEED TPKGNAEKND AG

Validated NT Sequence

tccctgcgtc gtttttttcc gcgtttccct tcggcgtgtc ctcctccccc tgagaagact tcttgcttcg ggtgttgtca ccatcatcgc aatgggcgtg tgctgttctc gacagtctta tcagtttgct ggggatcgtg agccgcaact ctttaccatc cacgacaccg agcacattga agagctcagg ccgtcgcatg tataacagtt tggccagccg cacacactcc gccgcggaga agaaaacgag gcacaacgta ttgccatcgg catcgttacc gcaagttttg acagagatgc tgctgctgct gttgccgtcc ctcctgcctg aaagcccgaa ggaggcggga gggtcattgt cgtcgttgcc gcgcttgccc tggtcaacgc cgttggtatt gggaacggcg atgttacggt tttggttcgg tcgggaggaa tgcatcatca cctcttgcac acaaccaaca tgatcctcaa agaacttgtg aacaaacgac gacgccgtat ctcgtggaag gttacgcaca gcaagaccga gtgaactaca gcgtcgcgtt cgctcctcca cgtccatcga ctcctgatac gcccgaagcg cgtcaataaa acccaaattt ggttccacgc gaggccgccg gcgtgtgacg tattgcagcg cctctgctac tgtccagccg ttgcggtaga tcagataggc gagcacaatg gtgacggagc ggctcttccc gcgttggcag tgtaccagag cgcggggaca aacactcgcg ccacgttgct tcgcatcata gtacgcttta cggacttgct caaggagtac gtgggttggg cgaaaaaact gctgaatgtt tgcctcggtg gtgtcatcca ccgcaaatgg atgaataaca atgctgcgat caacagacca atattcctca cgcgatacat taagaatagt tacaatattc tctcgacgca aaaattccgc atttgtggca tcacgcacac ccccaatgta aagaaagtca agaatttttg tcggtggctc acggccacac gtcgaaaatg aagagatttt tcccaaagaa gaataaggtg gtacagtagt gcaactactc aacaaactgg atgagctacc gtgc

Expected Protein Sequence

MAHHHHHHMH GSSSSLLSSC TTVLPYSSLG KISSFSTCGR EPPTKILDFL YIGGVRDATN AEFLRRENIV TILNVSREEY WSVDRSIVIH PFAVDDTTEA NIQQFFRPTH VILEQVRKAY YDAKQRGASV CPRALVHCQR GKSRSVTIVL AYLIYRNGWT VAEALQYVTR RRPRVEPNLG FIDALRAYQE SMDVEERTRR CSSLGLAVRN LPRDTASSFV HKFFEDHVGC VQEVMMHSSR PNQSRNIAAP NTNGVDQGKR GNDDNDPPAS FGLSGRRDGN SSSGIAVKTC SNDADGNTLC LVFFSAAECV RLAKLLYMRR PELFNVLGVV DGKELRLTIP SKLIRLSRTA HAHCDDGDNT RSKKSSQGEE DTPKGDAEKN DAGT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcacg gtagctcatc cagtttgttg agtagttgca ctactgtact accttattct tctttgggaa aaatctcgtc attttcgacg tgtggccgtg agccaccgac aaaaattctt gactttcttt acattggggg tgtgcgtgat gccacgaatg cggaattttt gcgtcgagag aatattgtaa ctattcttaa tgtatcgcgt gaggaatatt ggtctgttga tcgcagcatt gttattcatc catttgcagt ggatgacacc accgaggcaa acattcagca gttttttcgc ccaacccacg taatccttga gcaagtccgt aaagcgtact atgatgccaa gcaacgtggc gcgagtgttt gtccccgcgc tctggtgcac tgccaacgcg ggaagagccg ctccgtcacc attgtgctcg cctatctgat ctaccgcaac ggctggacag tagcagaggc gctgcaatac gtcacacgcc ggcggcctcg cgtggaacca aatttgggtt ttattgacgc gcttcgggcg tatcaggagt cgatggacgt ggaggagcga acgcgacgct gcagttcact cggtcttgcc gtgcgtaacc ttccacgaga tacggcgtcg tcgtttgttc acaagttctt tgaggatcat gttggttgtg tgcaggaggt gatgatgcac tcctcccgac cgaaccaaag ccgtaacatc gccgctccca ataccaacgg cgttgaccag ggcaagcgcg gcaacgacga caatgaccct cccgcctcct tcgggctttc aggcaggagg gacggcaaca gtagcagcgg catcgctgtc aaaacttgca gcaacgatgc cgatggcaat acgttgtgcc tcgttttctt ctctgcggcg gagtgtgtgc ggctggccaa actgttatac atgcgacggc ctgagctctt caatgtgctc ggtgtcgtgg atggtaaaga gttgcggctc acgatcccca gcaaactgat aagactgtcg agaacagcac acgcccattg cgatgatggt gacaacaccc gaagcaagaa gtcttctcag ggggaggagg acacgccgaa gggagacgcg gaaaaaaacg acgcagggac atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for TrcrA.18346.a.B1.PS01602

PURIFICATION DATe:	8/23/2012
CONCENTRATION:	21.42mg/ml
OBSERVED MW:	43kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	110µl
PERCENT IDENTITY:	96
PERCENT COVERAGE:	50

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCACG GTAGCTCATC CAGTTTGTTG AGTAGTTGCA CTACTGTACT ACCTTATTCT TCTTTGGGAA AAATCTCGTC ATTTTCGACG TGTGGCCGTG AGCCACCGAC AAAAATTCTT GACTTTCTTT ACATTGGGGG TGTGCGTGAT GCCACGAATG CGGAATTTTT GCGTCGAGAG AATATTGTAA CTATTCTTAA TGTATCGCGT GAGGAATATT GGTCTGTTGA TCGCAGCATT GTTATTCATC CATTTGCAGT GGATGACACC ACCGAGGCAA ACATTCAGCA GTTTTTTCGC CCAACCCACG TAATCCTTGA GCAAGTCCGT AAAGCGTACT ATGATGCCAA GCAACGTGGC GCGAGTGTTT GTCCCCGCGC TCTGGTGCAC TGCCAACGCG GGAAGAGCCG CTCCGTCACC ATTGTGCTCG CCTATCTGAT CTACCGCAAC GGCTGGACAG TAGCAGAGGC GCTGCAATAC GTCACACGCC GGCGGCCTCG CGTGGAACCA AATTTGGGTT TTATTGACGC GCTTCGGGCG TATCAGGAGT CGATGGACGT GGAGGAGCGA ACGCGACGCT GCAGTTCACT CGGTCTTGCC GTGCGTAACC TTCCACGAGA TACGGCGTCG TCGTTTGTTC ACAAGTTCTT TGAGGATCAT GTTGGTTGTG TGCAGGAGGT GATGATGCAC TCCTCCCGAC CGAACCAAAG CCGTAACATC GCCGCTCCCA ATACCAACGG CGTTGACCAG GGCAAGCGCG GCAACGACGA CAATGACCCT CCCGCCTCCT TCGGGCTTTC AGGCAGGAGG GACGGCAACA GTAGCAGCGG CATCGCTGTC AAAACTTGCA GCAACGATGC CGATGGCAAT ACGTTGTGCC TCGTTTTCTT CTCTGCGGCG GAGTGTGTGC GGCTGGCCAA ACTGTTATAC ATGCGACGGC CTGAGCTCTT CAATGTGCTC GGTGTCGTGG ATGGTAAAGA GTTGCGGCTC ACGATCCCCA GCAAACTGAT AAGACTGTCG AGAACAGCAC ACGCCCATTG CGATGATGGT GACAACACCC GAAGCAAGAA GTCTTCTCAG GGGGAGGAGG ACACGCCGAA GGGAGACGCG GAAAAAAACG ACGCAGGGAC ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT