TrcrA.18318.a

serine/threonine protein phosphatase-like protein, putative

CENTER ID: TrcrA.18318.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18318.a.B1.GE35428 full length 1 378
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053506465.50
RefSeq: XP_810029.1
UniProt: Q4D6M2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
CFFLRNVLVF SDLQLTPANI LCLGDYVDRG PYSLECLILL LSLKISHPSK VVMLRGNHED RVVCGDLLTY GSASFLAQCE ALYGVDEGRK LFKELTALFR HFPLAAELII PAVANNSGNN SNTTSSSINN NNNNNNNNHN HHYHNNNTNS PGDLAAASVS RLLAPQGRRR THEERVLCTH GGIPRFFSPP KEDDSLAFLR NEDFPRMLTL FPNNPFVRDD PEAQVELLGA VGQTPVSHAS STTAISEAML RKAWFVAFDL MWSDPTIHDP GDDDNESSAF ALSGNGHASI NEWGFGVNKR GSNVLTFFAK ALETFLQAHQ YSMLFRAHQE KAHGLRFSKS TKVLTIFSSS NYLGHGNGAG CVLVTADGEI QLVEKLSE
NT Sequence
tgcttttttc ttcgtaatgt tcttgtattt tccgacctgc aactcactcc cgcaaacatc ctctgcctcg gggactacgt ggaccgtgga ccgtactcac tcgaatgtct tattctgttg ctctcactga agatcagtca tccgtcgaag gtggtgatgc ttcgtggcaa tcatgaggat cgtgtggtgt gtggcgacct gttaacctat ggatctgctt cttttcttgc acaatgtgag gcgttatacg gcgtggatga ggggcggaaa ttgttcaagg aattgacggc tctttttcgc cacttccctc ttgctgcaga actcatcatt cctgcagtag caaacaacag tggcaacaac agtaatacta ccagtagtag tattaataat aataataaca ataataataa taatcataat catcattatc ataataataa caccaattct cccggtgatt tggctgcagc ttccgtttca aggttattgg caccacaggg tcgacggagg acccatgagg agcgcgtgtt gtgtacccac ggtggtatcc cgcgcttttt ttccccgcct aaggaggatg acagccttgc gtttctgcga aacgaggact ttccgcgcat gttgactttg ttccccaata atccctttgt gagggatgat ccggaggccc aggtggagct tctaggagct gtgggacaga cacctgtctc gcatgcgtca tcaacgactg ctataagcga agcgatgttg cgtaaagcat ggttcgttgc atttgatctt atgtggtctg accccacaat ccatgacccc ggcgatgatg ataatgagtc ctctgcattt gccttgtctg gaaacggcca tgcatctatc aatgagtggg gttttggtgt gaacaagcgc gggagtaatg tactcacgtt ttttgccaag gccttagaga cctttttgca ggcccaccag tacagcatgt tgtttcgcgc ccatcaagag aaggcgcatg ggctgcgttt tagcaagagc accaaggtat tgaccatttt tagcagctcc aactaccttg ggcacggcaa cggtgcaggt tgtgtgctgg tgactgccga tggtgaaatt cagcttgtcg agaagttgag tgagtga
Details for TrcrA.18318.a.B1.GE35428
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMC FFLRNVLVFS DLQLTPANIL CLGDYVDRGP YSLECLILLL SLKISHPSKV VMLRGNHEDR VVCGDLLTYG STSFLAQCEA LYGVDEGRKL FKELTALFRH FPLAAELIIP AVANNGGNNS NTTSSSNNNN NNNNNHNHHY HNNNTNSPGD LAAASVSRSL APQGRRRTHE ERVLCTHGGI PRFFSPPKED DSLAFLRHED FPRMLTLFPN NPFVRDDPEA QVELLGAVGQ TGVSHASSTT AISEAMLRKA WFVAFDLMWS XPTIHDPGDD DNESSATALS GNGHASINEW GFGVNKRGSN VLTFSAXXXX XFCXXTSTAX XXXPIKXXXX GCVXXXXXAS XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
ttttgtnnnn tttaagaagg aganatacca tggctcacca ccaccaccac catatgtgct tttttcttcg taatgttctt gtattttccg acctgcaact cactcccgca aacatcctct gcctcgggga ctacgtggac cgtggaccgt actcactcga atgtctcatt ctgttgctct cactgaagat cagtcatccg tcgaaggtgg tgatgcttcg tggcaatcat gaggatcgtg tggtatgtgg cgacctgtta acctatggat ccacttcttt tcttgcacaa tgtgaggcgt tatacggcgt ggatgagggg cggaaattgt tcaaggaatt gacggctctt tttcgccact tccctcttgc tgcagaactc atcattcctg cagtagcaaa caacggtggc aacaacagta atactaccag tagtagtaat aataataata acaataataa taatcataat catcattatc ataataataa caccaattct cccggtgatt tggctgcagc ttccgtttca aggtcattgg caccacaggg tcgacggagg acccatgagg agcgcgtgtt gtgtacccac ggtggtatcc cgcgcttttt ttccccgcct aaggaggatg acagccttgc gtttctgcga cacgaggact ttccgcgcat gttgactttg ttccccaata atccctttgt gagggatgat ccggaggccc aggtggagct gctaggggcc gtgggacaga caggtgtctc gcatgcgtca tcaacgactg ctataagcga agcgatgttg cgtaaagcat ggttcgttgc atttgatctt atgtggtctg ancccacaat ccatgacccc ggcgatgatg ataatgagtc ctctgcaact gccttgtctg gaaacggcca tgcatctatc aatgagtggg gttttggtgt gaacaagcgc gggagtaatg tgctcacntt ctctgcnnng nnttananan ctttttgcng nnccaccagt acagcatgnn gnnccgngcc catcaagana nnnnncangg gntgcgtnnn ncnngancnn cnngcntcnt tnncancnnn nnnnnnnnnn nnngnnnngn nnnggnnnnn nncnanggnn annnnnntnn nnnnnnnnan nannnnnnnn c
Expected Protein Sequence
MAHHHHHHMC FFLRNVLVFS DLQLTPANIL CLGDYVDRGP YSLECLILLL SLKISHPSKV VMLRGNHEDR VVCGDLLTYG SASFLAQCEA LYGVDEGRKL FKELTALFRH FPLAAELIIP AVANNSGNNS NTTSSSINNN NNNNNNNHNH HYHNNNTNSP GDLAAASVSR LLAPQGRRRT HEERVLCTHG GIPRFFSPPK EDDSLAFLRN EDFPRMLTLF PNNPFVRDDP EAQVELLGAV GQTPVSHASS TTAISEAMLR KAWFVAFDLM WSDPTIHDPG DDDNESSAFA LSGNGHASIN EWGFGVNKRG SNVLTFFAKA LETFLQAHQY SMLFRAHQEK AHGLRFSKST KVLTIFSSSN YLGHGNGAGC VLVTADGEIQ LVEKLSE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgct tttttcttcg taatgttctt gtattttccg acctgcaact cactcccgca aacatcctct gcctcgggga ctacgtggac cgtggaccgt actcactcga atgtcttatt ctgttgctct cactgaagat cagtcatccg tcgaaggtgg tgatgcttcg tggcaatcat gaggatcgtg tggtgtgtgg cgacctgtta acctatggat ctgcttcttt tcttgcacaa tgtgaggcgt tatacggcgt ggatgagggg cggaaattgt tcaaggaatt gacggctctt tttcgccact tccctcttgc tgcagaactc atcattcctg cagtagcaaa caacagtggc aacaacagta atactaccag tagtagtatt aataataata ataacaataa taataataat cataatcatc attatcataa taataacacc aattctcccg gtgatttggc tgcagcttcc gtttcaaggt tattggcacc acagggtcga cggaggaccc atgaggagcg cgtgttgtgt acccacggtg gtatcccgcg ctttttttcc ccgcctaagg aggatgacag ccttgcgttt ctgcgaaacg aggactttcc gcgcatgttg actttgttcc ccaataatcc ctttgtgagg gatgatccgg aggcccaggt ggagcttcta ggagctgtgg gacagacacc tgtctcgcat gcgtcatcaa cgactgctat aagcgaagcg atgttgcgta aagcatggtt cgttgcattt gatcttatgt ggtctgaccc cacaatccat gaccccggcg atgatgataa tgagtcctct gcatttgcct tgtctggaaa cggccatgca tctatcaatg agtggggttt tggtgtgaac aagcgcggga gtaatgtact cacgtttttt gccaaggcct tagagacctt tttgcaggcc caccagtaca gcatgttgtt tcgcgcccat caagagaagg cgcatgggct gcgttttagc aagagcacca aggtattgac catttttagc agctccaact accttgggca cggcaacggt gcaggttgtg tgctggtgac tgccgatggt gaaattcagc ttgtcgagaa gttgagtgag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat