TrcrA.18314.a

calpain-like cysteine peptidase, putative

CENTER ID: TrcrA.18314.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18314.a.B1.GE35422 full length 1 349
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18314.a.B1.PW36549 full length 1 349

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053510957.9
RefSeq: XP_802398.1
UniProt: Q4CL00

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAENQWEEL EDLPHRNKEA SAEIGNDLQD GSHPDADKAN GEVGEIQETS EYPVEKAEAD NQPEFCDQEV QEPRPHTPEE EYPAEEPNTY PEEEPRNEEV NKREVPVEEG VVRGENKESN EDEFPPHTQE VHHLVKPRKT PFKKPIRWDP IEYDLREDSD DEFEMATALR PVYEEEEGFG VPKFKNGEPD YQGEYLSCFD EPNLLYRILN REEKTWAFYN DTRHYEMHVR FIFGKNSVLE ALGNTKIHTR SDGEHVAEVF VYPGETEMFI KGRVNGFTSK LRAVPLSEEY HQRRQEVAED AIQREVAQIK AIVGDERSAE KVLEACLENN LPFVDLEFPP CQASLDTGA
NT Sequence
atgtctgccg agaatcagtg ggaagaactt gaagatttac cccaccgaaa taaggaggct tcagcggaaa ttggcaatga tctccaagac gggagccacc ccgacgctga caaagccaat ggtgaagtag gcgaaatcca agagacatct gaatacccag tggagaaggc tgaagccgat aaccaaccgg aattttgtga ccaagaagta caggaaccca ggccccacac tccggaagag gagtatcccg cagaagaacc aaatacttat cctgaagagg agcctcgcaa tgaggaagta aataagcgtg aggtgccagt ggaagaaggt gttgtgcgtg gggagaacaa ggaaagcaat gaagacgaat ttccacctca cacacaggaa gttcatcatc tcgtcaagcc aaggaaaacg ccgtttaaga aaccaattcg ttgggatcct attgagtacg atttgcgtga agacagtgac gatgaatttg agatggcaac agcattgcgc ccagtctatg aggaggaaga gggcttcggg gtgcccaaat ttaaaaatgg cgagccggac tatcaaggtg agtatctttc ctgttttgat gagcccaatt tgttgtaccg cattttgaac cgcgaagaaa aaacttgggc cttttacaat gatactcgcc attatgaaat gcacgtgcgg ttcatctttg gtaagaactc cgtgctagag gcacttggaa atacgaaaat tcatactcgg tccgatggtg agcatgtggc tgaggtcttt gtttaccccg gagaaacgga aatgttcatt aagggcaggg tgaatggttt cacaagcaag ctccgcgcgg ttcctttgtc ggaggaatac catcaacgcc gtcaagaagt tgctgaggac gccattcaac gtgaagtggc tcagattaag gcaattgttg gggatgagag gagtgcagag aaggttttgg aggcttgttt ggagaataat ctcccttttg tggacctgga gtttccaccc tgccaggcat cattggatac tggcgcg
Details for TrcrA.18314.a.B1.GE35422
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 41kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS AENQWEELED LPQQNKEASA EIDNDLQDGS HSDADKANGE VGEIQETSEY PVEKAEANNQ PEFCDQEVQE PRPHPPEEEY PAEEPNTYPE EEPHNEEVNK REVPVEEGVV RGENKESNED EFPPHTQEVH HLVKPRKTPL KKPTRWDPIE YDLREDSDDE FEMATALRPV YEEEEEFGVP KFKNGEPDYQ GEYLSCFDEP NLLYRILNRE EKTWAFYNDT RHYEMHVRFI FGKNSVLEAL GNTKIHTRSD GEHVAEVFVY PGETEMFIKG RVNGFTSKLR AVPLSEEYHQ RRQEVAEDAI QREVAQIKAI VGDERSAEKV LEACLENNLP FVDLEFPPCQ ASLDTGA
Validated NT Sequence
ttactcactt acgcgccagt atccaatgat gcctggcagg gtggaaactc caggtccaca aaagggagat tattctccaa acaagcctcc aaaaccttct ctgcactcct ctcatcccca acaattgcct taatctgagc cacttcacgt tgaatggcgt cctcagcaac ttcttgacgg cgttgatggt attcctccga caaaggaacc gcgcggagct tgcttgtgaa accattaacc ctgcccttaa tgaacatttc cgtttctccg gggtaaacaa agacctcagc cacatgctca ccatcggacc gagtatgaat tttcgtattt ccaagtgcct ctagcacgga gttcttacca aagatgaacc gcacgtgcat ttcataatgg cgagtatcat tgtaaaaggc ccaagttttt tcttcgcggt tcaaaatgcg gtacaacaaa ttgggctcat caaaacagga aagatactca ccttgatagt ctggctcgcc atttttgaac ttgggcaccc cgaattcctc ttcctcctca tagactgggc gcaatgctgt agccatctca aattcatcgt cactgtcttc acgcaaatcg tactcaatag gatcccaacg agttggtttc ttaagcggcg ttttccttgg cttgacgaga tgatgaactt cctgtgtgtg aggtggaaat tcgtcttcat tgctttcctt gttctcccca cgcacaacac cctcttccac tggcacctca cgcttattta cttcctcatt gtggggctcc tcttcaggat aagtatttgg ttcttctgcg gggtactcct cttccggagg gtggggcctg ggttcctgta cttcttggtc acaaaattcc ggttggttat tggcttcagc cttctccact gggtattcag atgtctcttg gatttcgcct acttcaccat tggctttgtc agcgtcggag tggctcccgt cttggagatc attgtcaatt tccgctgaag cctccttatt ttgttggggt aaatcttcaa gttcttccca ctgattctcg gcagacatat ggtggtggtg gtggtgagcc atggtatatc tcctt
Expected Protein Sequence
MAHHHHHHMS AENQWEELED LPHRNKEASA EIGNDLQDGS HPDADKANGE VGEIQETSEY PVEKAEADNQ PEFCDQEVQE PRPHTPEEEY PAEEPNTYPE EEPRNEEVNK REVPVEEGVV RGENKESNED EFPPHTQEVH HLVKPRKTPF KKPIRWDPIE YDLREDSDDE FEMATALRPV YEEEEGFGVP KFKNGEPDYQ GEYLSCFDEP NLLYRILNRE EKTWAFYNDT RHYEMHVRFI FGKNSVLEAL GNTKIHTRSD GEHVAEVFVY PGETEMFIKG RVNGFTSKLR AVPLSEEYHQ RRQEVAEDAI QREVAQIKAI VGDERSAEKV LEACLENNLP FVDLEFPPCQ ASLDTGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg ccgagaatca gtgggaagaa cttgaagatt taccccaccg aaataaggag gcttcagcgg aaattggcaa tgatctccaa gacgggagcc accccgacgc tgacaaagcc aatggtgaag taggcgaaat ccaagagaca tctgaatacc cagtggagaa ggctgaagcc gataaccaac cggaattttg tgaccaagaa gtacaggaac ccaggcccca cactccggaa gaggagtatc ccgcagaaga accaaatact tatcctgaag aggagcctcg caatgaggaa gtaaataagc gtgaggtgcc agtggaagaa ggtgttgtgc gtggggagaa caaggaaagc aatgaagacg aatttccacc tcacacacag gaagttcatc atctcgtcaa gccaaggaaa acgccgttta agaaaccaat tcgttgggat cctattgagt acgatttgcg tgaagacagt gacgatgaat ttgagatggc aacagcattg cgcccagtct atgaggagga agagggcttc ggggtgccca aatttaaaaa tggcgagccg gactatcaag gtgagtatct ttcctgtttt gatgagccca atttgttgta ccgcattttg aaccgcgaag aaaaaacttg ggccttttac aatgatactc gccattatga aatgcacgtg cggttcatct ttggtaagaa ctccgtgcta gaggcacttg gaaatacgaa aattcatact cggtccgatg gtgagcatgt ggctgaggtc tttgtttacc ccggagaaac ggaaatgttc attaagggca gggtgaatgg tttcacaagc aagctccgcg cggttccttt gtcggaggaa taccatcaac gccgtcaaga agttgctgag gacgccattc aacgtgaagt ggctcagatt aaggcaattg ttggggatga gaggagtgca gagaaggttt tggaggcttg tttggagaat aatctccctt ttgtggacct ggagtttcca ccctgccagg catcattgga tactggcgcg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for TrcrA.18314.a.B1.PW36549
PURIFICATION DATe: 8/7/2012
CONCENTRATION: 19.27mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS AENQWEELED LPQQNKEASA EIDNDLQDGS HSDADKANGE VGEIQETSEY PVEKAEANNQ PEFCDQEVQE PRPHPPEEEY PAEEPNTYPE EEPHNEEVNK REVPVEEGVV RGENKESNED EFPPHTQEVH HLVKPRKTPL KKPTRWDPIE YDLREDSDDE FEMATALRPV YEEEEEFGVP KFKNGEPDYQ GEYLSCFDEP NLLYRILNRE EKTWAFYNDT RHYEMHVRFI FGKNSVLEAL GNTKIHTRSD GEHVAEVFVY PGETEMFIKG RVNGFTSKLR AVPLSEEYHQ RRQEVAEDAI QREVAQIKAI VGDERSAEKV LEACLENNLP FVDLEFPPCQ ASLDTGA
Validated NT Sequence
ttactcactt acgcgccagt atccaatgat gcctggcagg gtggaaactc caggtccaca aaagggagat tattctccaa acaagcctcc aaaaccttct ctgcactcct ctcatcccca acaattgcct taatctgagc cacttcacgt tgaatggcgt cctcagcaac ttcttgacgg cgttgatggt attcctccga caaaggaacc gcgcggagct tgcttgtgaa accattaacc ctgcccttaa tgaacatttc cgtttctccg gggtaaacaa agacctcagc cacatgctca ccatcggacc gagtatgaat tttcgtattt ccaagtgcct ctagcacgga gttcttacca aagatgaacc gcacgtgcat ttcataatgg cgagtatcat tgtaaaaggc ccaagttttt tcttcgcggt tcaaaatgcg gtacaacaaa ttgggctcat caaaacagga aagatactca ccttgatagt ctggctcgcc atttttgaac ttgggcaccc cgaattcctc ttcctcctca tagactgggc gcaatgctgt agccatctca aattcatcgt cactgtcttc acgcaaatcg tactcaatag gatcccaacg agttggtttc ttaagcggcg ttttccttgg cttgacgaga tgatgaactt cctgtgtgtg aggtggaaat tcgtcttcat tgctttcctt gttctcccca cgcacaacac cctcttccac tggcacctca cgcttattta cttcctcatt gtggggctcc tcttcaggat aagtatttgg ttcttctgcg gggtactcct cttccggagg gtggggcctg ggttcctgta cttcttggtc acaaaattcc ggttggttat tggcttcagc cttctccact gggtattcag atgtctcttg gatttcgcct acttcaccat tggctttgtc agcgtcggag tggctcccgt cttggagatc attgtcaatt tccgctgaag cctccttatt ttgttggggt aaatcttcaa gttcttccca ctgattctcg gcagacatat ggtggtggtg gtggtgagcc atggtatatc tcctt
Expressed Protein Sequence
MAHHHHHHMS AENQWEELED LPHRNKEASA EIGNDLQDGS HPDADKANGE VGEIQETSEY PVEKAEADNQ PEFCDQEVQE PRPHTPEEEY PAEEPNTYPE EEPRNEEVNK REVPVEEGVV RGENKESNED EFPPHTQEVH HLVKPRKTPF KKPIRWDPIE YDLREDSDDE FEMATALRPV YEEEEGFGVP KFKNGEPDYQ GEYLSCFDEP NLLYRILNRE EKTWAFYNDT RHYEMHVRFI FGKNSVLEAL GNTKIHTRSD GEHVAEVFVY PGETEMFIKG RVNGFTSKLR AVPLSEEYHQ RRQEVAEDAI QREVAQIKAI VGDERSAEKV LEACLENNLP FVDLEFPPCQ ASLDTGA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTG CCGAGAATCA GTGGGAAGAA CTTGAAGATT TACCCCACCG AAATAAGGAG GCTTCAGCGG AAATTGGCAA TGATCTCCAA GACGGGAGCC ACCCCGACGC TGACAAAGCC AATGGTGAAG TAGGCGAAAT CCAAGAGACA TCTGAATACC CAGTGGAGAA GGCTGAAGCC GATAACCAAC CGGAATTTTG TGACCAAGAA GTACAGGAAC CCAGGCCCCA CACTCCGGAA GAGGAGTATC CCGCAGAAGA ACCAAATACT TATCCTGAAG AGGAGCCTCG CAATGAGGAA GTAAATAAGC GTGAGGTGCC AGTGGAAGAA GGTGTTGTGC GTGGGGAGAA CAAGGAAAGC AATGAAGACG AATTTCCACC TCACACACAG GAAGTTCATC ATCTCGTCAA GCCAAGGAAA ACGCCGTTTA AGAAACCAAT TCGTTGGGAT CCTATTGAGT ACGATTTGCG TGAAGACAGT GACGATGAAT TTGAGATGGC AACAGCATTG CGCCCAGTCT ATGAGGAGGA AGAGGGCTTC GGGGTGCCCA AATTTAAAAA TGGCGAGCCG GACTATCAAG GTGAGTATCT TTCCTGTTTT GATGAGCCCA ATTTGTTGTA CCGCATTTTG AACCGCGAAG AAAAAACTTG GGCCTTTTAC AATGATACTC GCCATTATGA AATGCACGTG CGGTTCATCT TTGGTAAGAA CTCCGTGCTA GAGGCACTTG GAAATACGAA AATTCATACT CGGTCCGATG GTGAGCATGT GGCTGAGGTC TTTGTTTACC CCGGAGAAAC GGAAATGTTC ATTAAGGGCA GGGTGAATGG TTTCACAAGC AAGCTCCGCG CGGTTCCTTT GTCGGAGGAA TACCATCAAC GCCGTCAAGA AGTTGCTGAG GACGCCATTC AACGTGAAGT GGCTCAGATT AAGGCAATTG TTGGGGATGA GAGGAGTGCA GAGAAGGTTT TGGAGGCTTG TTTGGAGAAT AATCTCCCTT TTGTGGACCT GGAGTTTCCA CCCTGCCAGG CATCATTGGA TACTGGCGCG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT