TrcrA.18247.a

serine/threonine protein kinase, putative

CENTER ID: TrcrA.18247.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18247.a.B1.GE35419 full length 1 646
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053510329.210
RefSeq: XP_821198.1
UniProt: Q4E3J2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHSRRDVDSV SREPSSDFQY IGNYAMGETI GKGSFGKVRK GRHLPTGETV AIKILNRNKL NNAKMGKRVY REMKILKLFS HPNICRLYEV ITTPTDMYLI MEYVEGGELY DYIVKRRMLK EDVGRYILQQ IVCALEYCHH FLVVHRDLKP ENILLGPGLQ VKLIDFGLAN IMKDNEFLAS SCGSPNYAAP EILSGKLYFG PEVDVWSCGV ILYALLCGCL PFDEESIPLL FSKIKKGQYQ IPPHVSHGAR ELIEQILVVD PLMRLTIPQI RDNAWFNTNL PMRLSYNETI FLTGQKRISP KVASRAAKIL GMRDRDLRGE IEEGHGSGYV AYKILLDAER AKQIIAEAES FAVEDDLWGG SKRLATHVSK ATERELNLGL LLTQSPAMET LLEVGDATSN KRAYNCGNFV PASVQETKAF AMSVHTPVSN GTFSISKRPE SLTDATNPFG SGFVGSGSSV QRPSALSTSA RQGSNTNLAA SVGDRQHVGS VAKSHMVYTK EEEEFIVRNN VGWRLGLMSD FNAAVSQNVL YSTLTSFEME WKVLSPFRLL VRTTPGTWAK IGLAPTRSPL TPLSMDPPEL LNEHSPNPAI TSYTASPPNV ILALYLLRIH EKHDRGYLLD FSVVKGSLLA LDVVLRLSTT LVHRMG
NT Sequence
atgcattcca ggcgggatgt tgactctgtg tcacgggaac cgtcaagtga ttttcagtac attggcaact atgccatggg cgagaccatt gggaaaggga gctttgggaa ggtgaggaaa ggtcgtcacc tcccaacagg ggaaactgtc gctattaaga tactgaaccg taacaagttg aataatgcca aaatgggcaa aagagtctat cgagagatga agatactcaa acttttttcc catcccaaca tctgccgact ctacgaggtt ataacaaccc ctaccgacat gtatcttatt atggaatatg ttgagggcgg agagttgtac gactacatcg tgaagaggcg tatgttgaaa gaagatgtag gccgttatat cttgcaacaa attgtctgtg cattggagta ttgccatcat tttcttgttg tgcatcggga tcttaagccg gaaaatattt tgctggggcc cgggctgcag gtaaaactca ttgattttgg acttgccaac attatgaagg acaatgaatt tttggcgagt tcttgtggat cgcccaatta cgccgctccg gagattcttt cgggcaagtt gtactttggt ccagaggtgg atgtgtggtc atgtggagtc attttgtacg ccctgctgtg cgggtgcctc ccgtttgatg aagaaagcat cccgcttctt ttttccaaaa tcaaaaaggg gcagtatcaa attcctcctc atgtctctca tggggcgcga gaactcattg aacaaatttt agttgttgac cccctcatgc gtcttacaat acctcagatc cgagataatg cgtggtttaa cacaaatctt cccatgcgtc tttcctacaa tgaaactatt tttttgacgg gacaaaagcg catctcgcca aaggtggcaa gccgggcggc gaagattctt ggcatgcgcg acagagattt gcgcggtgaa attgaggagg gccatggctc cgggtatgtt gcttacaaaa tccttttgga tgcagagcga gcgaaacaga tcattgcgga ggcagagtcg tttgcggtgg aggatgacct ttggggtggc tcaaaacgac tggcgacaca tgtatccaag gcaaccgaac gtgaactcaa tcttggactc ctcctcacac agagtcctgc catggagaca ctccttgaag ttggtgatgc cacctctaac aaacgtgcct ataattgcgg taattttgtt ccagcgtcgg tacaagaaac aaaggcgttt gcaatgtctg tgcatacacc cgtctctaat ggtacgttca gtatttcgaa gcggccagaa tcccttaccg acgctactaa tccgtttggt tcgggttttg ttggaagtgg cagtagtgta cagcgtccgt ctgcccttag cacgagcgca cgtcaaggat ccaatacgaa tctcgctgca agcgtgggtg atcgtcaaca cgttgggtct gtcgcaaagt cccacatggt gtacacgaag gaggaggaag aatttattgt gaggaacaac gttggctggc gtcttggttt gatgagtgac ttcaacgccg ctgtctcgca gaatgttctt tatagcactc taacttcctt tgagatggag tggaaggtat tgtcaccatt tcgtctgctg gtccgcacaa caccggggac gtgggctaaa attggtctcg cgccaacaag gtcaccgctg acaccacttt cgatggatcc accggagctt ttgaatgaac atagcccaaa tccagcaatt actagttaca ctgcttctcc tccaaatgtg attctagccc tataccttct tcgtatacac gagaagcatg acagggggta cttgttggac ttcagcgtgg tgaagggttc acttctagcg cttgatgttg tgctacgtct ttcaacgacg ctcgttcatc gaatgggtta a
Details for TrcrA.18247.a.B1.GE35419
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 72kDa
OBSERVED MW: 72kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 78
PERCENT COVERAGE: 60
Validated AA Sequence
MAHHHHHHMH SRRDVDSVPR EPSSDVQYIG NYAMGETIGK GSFGKVRKGR HLPTGETVAI KILNRNKLNN AKMGKRVYRE MKILKLFSHP NICRLYEVIK TPTDMYLIME YVEGGELYDY IVKRRMLKED VGRYILQQIV CALEYCHHFL VVHRDLKPEN ILLGPGLQVK LIDFGLANIM KDNEFLASSC GSPNYAAPEI LSGKLYFGPE VDVWSCGVIL YALLCGCLPF DEESIPLLFS KIKKGQYQIP PHVSHGAREL IEQILVVDPL MRLTIPQIXD NAXFNTNLPM RLSYNETIFL TGQXASRQRW QAGRRRFXGM RDRXLRGEXE XGXXLXVXXX XXXFXXXXXX XXHXXXXXXX XXXXGXXXXX XXXXXXXXXX XXXXXXXXXX XXXX
Validated NT Sequence
attttnttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgcat tccaggcggg atgttgactc tgtgccgcgg gaaccgtcaa gtgatgttca gtacattggc aactatgcca tgggcgagac cattgggaaa gggagctttg ggaaggtgag gaagggtcgt cacctcccaa caggggaaac tgtcgccatt aagatactga accgtaacaa gttgaataat gccaaaatgg gcaaaagagt ctatcgagag atgaagatac tcaaactttt ttcccatccc aacatctgcc gactctacga ggttataaaa acccctaccg acatgtatct tattatggaa tatgttgagg gcggagagtt gtacgactac atcgtgaaga ggcgtatgtt gaaagaagat gtaggccgtt atatcttgca acaaattgtc tgtgcattgg agtattgcca tcattttctt gttgtgcatc gagatcttaa gccggaaaat attttactag ggcccgggct gcaggtaaaa ctcattgatt ttggacttgc caacattatg aaggacaatg aatttttggc gagttcttgt ggatcgccca attacgccgc tccggagatt ctttcgggca agttgtactt tggtccagag gtggatgtat ggtcatgtgg agtcattttg tacgccctgc tgtgcgggtg cctcccgttt gatgaagaaa gcatcccgct tcttttttcc aaaatcaaaa aggggcagta tcaaattcct cctcatgtct ctcatggggc gcgagaactc attgaacaaa ttttagttgt tgaccccctc atgcgtctta caatacctca gatccnagat aatgcgtgnt ttaacacaaa tcttcccatg cgtctttcct acaatgaaac tatttttttg acgggacaaa ncgcatctcg ccaaaggtgg caagccgggc ggcgaagatt cntnggcatg cgcgacagan atttgcgcgg tgaaantgag ganggnnann ggctnnnggt atgnngcttn nnnnannnnt ttnnnngcnn ancnnncnaa annnatcatt gnnngnnnan tcnntnnnnn nnnnangncn ttggggnaaa nnacnggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntnnnncnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tnaa
Expected Protein Sequence
MAHHHHHHMH SRRDVDSVSR EPSSDFQYIG NYAMGETIGK GSFGKVRKGR HLPTGETVAI KILNRNKLNN AKMGKRVYRE MKILKLFSHP NICRLYEVIT TPTDMYLIME YVEGGELYDY IVKRRMLKED VGRYILQQIV CALEYCHHFL VVHRDLKPEN ILLGPGLQVK LIDFGLANIM KDNEFLASSC GSPNYAAPEI LSGKLYFGPE VDVWSCGVIL YALLCGCLPF DEESIPLLFS KIKKGQYQIP PHVSHGAREL IEQILVVDPL MRLTIPQIRD NAWFNTNLPM RLSYNETIFL TGQKRISPKV ASRAAKILGM RDRDLRGEIE EGHGSGYVAY KILLDAERAK QIIAEAESFA VEDDLWGGSK RLATHVSKAT ERELNLGLLL TQSPAMETLL EVGDATSNKR AYNCGNFVPA SVQETKAFAM SVHTPVSNGT FSISKRPESL TDATNPFGSG FVGSGSSVQR PSALSTSARQ GSNTNLAASV GDRQHVGSVA KSHMVYTKEE EEFIVRNNVG WRLGLMSDFN AAVSQNVLYS TLTSFEMEWK VLSPFRLLVR TTPGTWAKIG LAPTRSPLTP LSMDPPELLN EHSPNPAITS YTASPPNVIL ALYLLRIHEK HDRGYLLDFS VVKGSLLALD VVLRLSTTLV HRMG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatt ccaggcggga tgttgactct gtgtcacggg aaccgtcaag tgattttcag tacattggca actatgccat gggcgagacc attgggaaag ggagctttgg gaaggtgagg aaaggtcgtc acctcccaac aggggaaact gtcgctatta agatactgaa ccgtaacaag ttgaataatg ccaaaatggg caaaagagtc tatcgagaga tgaagatact caaacttttt tcccatccca acatctgccg actctacgag gttataacaa cccctaccga catgtatctt attatggaat atgttgaggg cggagagttg tacgactaca tcgtgaagag gcgtatgttg aaagaagatg taggccgtta tatcttgcaa caaattgtct gtgcattgga gtattgccat cattttcttg ttgtgcatcg ggatcttaag ccggaaaata ttttgctggg gcccgggctg caggtaaaac tcattgattt tggacttgcc aacattatga aggacaatga atttttggcg agttcttgtg gatcgcccaa ttacgccgct ccggagattc tttcgggcaa gttgtacttt ggtccagagg tggatgtgtg gtcatgtgga gtcattttgt acgccctgct gtgcgggtgc ctcccgtttg atgaagaaag catcccgctt cttttttcca aaatcaaaaa ggggcagtat caaattcctc ctcatgtctc tcatggggcg cgagaactca ttgaacaaat tttagttgtt gaccccctca tgcgtcttac aatacctcag atccgagata atgcgtggtt taacacaaat cttcccatgc gtctttccta caatgaaact atttttttga cgggacaaaa gcgcatctcg ccaaaggtgg caagccgggc ggcgaagatt cttggcatgc gcgacagaga tttgcgcggt gaaattgagg agggccatgg ctccgggtat gttgcttaca aaatcctttt ggatgcagag cgagcgaaac agatcattgc ggaggcagag tcgtttgcgg tggaggatga cctttggggt ggctcaaaac gactggcgac acatgtatcc aaggcaaccg aacgtgaact caatcttgga ctcctcctca cacagagtcc tgccatggag acactccttg aagttggtga tgccacctct aacaaacgtg cctataattg cggtaatttt gttccagcgt cggtacaaga aacaaaggcg tttgcaatgt ctgtgcatac acccgtctct aatggtacgt tcagtatttc gaagcggcca gaatccctta ccgacgctac taatccgttt ggttcgggtt ttgttggaag tggcagtagt gtacagcgtc cgtctgccct tagcacgagc gcacgtcaag gatccaatac gaatctcgct gcaagcgtgg gtgatcgtca acacgttggg tctgtcgcaa agtcccacat ggtgtacacg aaggaggagg aagaatttat tgtgaggaac aacgttggct ggcgtcttgg tttgatgagt gacttcaacg ccgctgtctc gcagaatgtt ctttatagca ctctaacttc ctttgagatg gagtggaagg tattgtcacc atttcgtctg ctggtccgca caacaccggg gacgtgggct aaaattggtc tcgcgccaac aaggtcaccg ctgacaccac tttcgatgga tccaccggag cttttgaatg aacatagccc aaatccagca attactagtt acactgcttc tcctccaaat gtgattctag ccctatacct tcttcgtata cacgagaagc atgacagggg gtacttgttg gacttcagcg tggtgaaggg ttcacttcta gcgcttgatg ttgtgctacg tctttcaacg acgctcgttc atcgaatggg ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat