TrcrA.18246.b

folylpolyglutamate synthetase

CENTER ID: TrcrA.18246.b
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18246.b.B1.GE35414 full length 1 487
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18246.b.B1.PS01629 full length 1 487

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053509715.70
RefSeq: XP_813449.1
UniProt: Q4DGE9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSMNAAVCGR TFNDVVRLIE SWAIGKVASS CNNLETTRLF IDRLNMQGYL DKLRFVHVAG TKGKGTTAAY TAALLQAYGL RVGLFTSPHL VDVRERILVN NAFLPKDVFA RQFFKLVDRI ADLSNSESQI SRDLSKRTSF FRFLFLLSLY IFAEELVDVV VVEVGVGGRI DATNIISPEV TVITSLGLDH TEVLGNTIEE IALEKAGIMK PGVICYTSPQ EWHPSTLTVM EKHARGQQTP FVVVDEKTLP IFNWPPLAIG GNHVIEDSKL ALLAARYMAG IPPTLPLSGA ERQVLQTMTL PGRSQVLSIH GGTGATLYLD CAHTYESLTC ATRWFMEESR SLTGDANPRR VLVFYSTRDA THVLKSFMPF IHCFSKMIAA FVVNPRKATG NTADGRYDYA RGEVAAVTSC WKEMYREVPC IPCKAPFSSL EELIDVAVPA ATEREDALKP VQVFVTGSFF LLSDIMGLLQ AHESKFQTKL KEDSKED
NT Sequence
atgagtatga acgctgcggt ttgtgggagg acgtttaacg acgtggtgcg tctaatcgag agctgggcaa taggaaaggt ggcctcctct tgcaacaacc ttgagaccac aaggcttttt atagaccgtc tcaatatgca gggttatctt gacaagcttc gttttgtcca tgtggcggga acaaagggaa aggggacaac cgcggcgtac accgcggcgc tcctgcaggc gtatgggctt agggttggcc ttttcacctc cccacacctt gtggatgtcc gggagcgcat tttggtgaat aatgcctttt tgcccaagga tgtattcgcg aggcaatttt tcaaattggt tgaccgcatt gcggatcttt caaactcgga gagccaaatt tcccgcgatc tctcgaaacg cacaagcttc tttcgtttcc tgtttttact ctcgctgtac atcttcgctg aggagttggt ggatgtggtg gttgtggagg tgggcgtggg gggccgcatc gacgccacaa atatcatttc gcccgaggtg actgtcataa cttcactggg tctcgatcac acagaagttt tgggaaacac catagaggaa attgcccttg aaaaggccgg tattatgaag cccggtgtta tttgctacac ctcaccgcag gagtggcacc cgtccacgct gactgtgatg gagaaacatg ctcgtggcca gcagactccc tttgtggttg tggacgagaa gacgctgccc atattcaatt ggcctcccct ggctattggg ggaaaccacg tcattgagga ctccaaactg gcgctgctgg cggccaggta tatggcagga attcccccta ccctgcctct gagtggcgct gaacgtcaag tgttgcagac gatgactctt ccaggccgtt cgcaggtgct gtcgatccac ggcggaacgg gcgcaacgct ttaccttgac tgcgcccaca cgtatgagag tttgacgtgt gccacccgct ggtttatgga ggaaagccgt tccttaactg gagatgcgaa tccacgtcgc gtgcttgttt tttactccac tcgggatgcg acgcatgtcc ttaagtcgtt tatgcctttt attcactgtt tttccaagat gattgccgca tttgtggtga atccacggaa ggcgactgga aacacggcgg atggaaggta cgattatgcg cgaggcgaag tagcagccgt gacgagttgc tggaaagaga tgtatcgtga ggttccatgt attccctgta aagccccgtt ttcctcgctg gaagaactga ttgatgtggc cgttcccgct gcaaccgaac gcgaggatgc cttgaaaccg gtgcaggtct ttgttaccgg gtccttcttt ttgctttcag acatcatggg gctgctccag gcccacgaat ccaaatttca aaccaaattg aaggaagata gtaaggagga ctag
Details for TrcrA.18246.b.B1.GE35414
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS MNAAVCGRTF NDVVRLIESW AIGKVASSCN NLETTRLFID RLNMQGYLDK LRFVHVAGTK GKGTTAAYTA ALLQAYGLRV GLFTSPHLVD VRERILVNNA FLPKDVFARQ FFKLVDRIAD LSNSESQISR DLSKRTSFFR FLFLLSLYIF AEELVDVVVV EVGVGGRIDA TNIISPEVTV ITSLGLDHTE VLGNTIEEIA LEKAGIMKPG VICYTSPQEW HPSTLTVMEK HARGQQTPFV VVDEKTLPIF NWPPLAIGGN HVIEDSKLAL LAARYMAGIP PTLPLSGAER QVLQTMTLPG RSQVLSIHGG TGATLYLDCA HTYESLTCAT RWFMEESRSL TGDANPRRVL VFYSTRDATH VLKSFMPFIH CFSKMIAAFV VNPRKATGNT ADGRYDYARG EVAAVTSCWK EMYREVPCIP CKAPFSSLEE LIDVAVPAAT EREDALKPVQ VFVTGSFFLL SDIMGLLQAH ESKFQTKLKE DSKED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta tgaacgctgc ggtttgtggg aggacgttta acgacgtggt gcgtctaatc gagagctggg caataggaaa ggtggcctcc tcttgcaaca accttgagac cacaaggctt tttatagacc gtctcaatat gcagggttat cttgacaagc ttcgttttgt ccatgtggcg ggaacaaagg gaaaggggac aaccgcggcg tacaccgcgg cgctcctgca ggcgtatggg cttagggttg gccttttcac ctccccacac cttgtggatg tccgggagcg cattttggtg aataatgcct ttttgcccaa ggatgtattc gcgaggcaat ttttcaaatt ggttgaccgc attgcggatc tttcaaactc ggagagccaa atttcccgcg atctctcgaa acgcacaagc ttctttcgtt tcctgttttt actctcgctg tacatcttcg ctgaggagtt ggtggatgtg gtggttgtgg aggtgggcgt ggggggccgc atcgacgcca caaatatcat ttcgcccgag gtgactgtca taacttcact gggtctcgat cacacagaag ttttgggaaa caccatagag gaaattgccc ttgaaaaggc cggtattatg aagcccggtg ttatttgcta cacctcaccg caggagtggc acccgtccac gctgactgtg atggagaaac atgctcgtgg ccagcagact ccctttgtgg ttgtggacga gaagacgctg cccatattca attggcctcc cctggctatt gggggaaacc acgtcattga ggactccaaa ctggcgctgc tggcggccag gtatatggca ggaattcccc ctaccctgcc tctgagtggc gctgaacgtc aagtgttgca gacgatgact cttccaggcc gttcgcaggt gctgtcgatc cacggcggaa cgggcgcaac gctttacctt gactgcgccc acacgtatga gagtttgacg tgtgccaccc gctggtttat ggaggaaagc cgttccttaa ctggagatgc gaatccacgt cgcgtgcttg ttttttactc cactcgggat gcgacgcatg tccttaagtc gtttatgcct tttattcact gtttttccaa gatgattgcc gcatttgtgg tgaatccacg gaaggcgact ggaaacacgg cggatggaag gtacgattat gcgcgaggcg aagtagcagc cgtgacgagt tgctggaaag agatgtatcg tgaggttcca tgtattccct gtaaagcccc gttttcctcg ctggaagaac tgattgatgt ggccgttccc gctgcaaccg aacgcgagga tgccttgaaa ccggtgcagg tctttgttac cgggtccttc tttttgcttt cagacatcat ggggctgctc caggcccacg aatccaaatt tcaaaccaaa ttgaaggaag atagtaagga ggactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for TrcrA.18246.b.B1.PS01629
PURIFICATION DATe: 9/20/2012
CONCENTRATION: 33.89mg/ml
OBSERVED MW: 49kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS MNAAVCGRTF NDVVRLIESW AIGKVASSCN NLETTRLFID RLNMQGYLDK LRFVHVAGTK GKGTTAAYTA ALLQAYGLRV GLFTSPHLVD VRERILVNNA FLPKDVFARQ FFKLVDRIAD LSNSESQISR DLSKRTSFFR FLFLLSLYIF AEELVDVVVV EVGVGGRIDA TNIISPEVTV ITSLGLDHTE VLGNTIEEIA LEKAGIMKPG VICYTSPQEW HPSTLTVMEK HARGQQTPFV VVDEKTLPIF NWPPLAIGGN HVIEDSKLAL LAARYMAGIP PTLPLSGAER QVLQTMTLPG RSQVLSIHGG TGATLYLDCA HTYESLTCAT RWFMEESRSL TGDANPRRVL VFYSTRDATH VLKSFMPFIH CFSKMIAAFV VNPRKATGNT ADGRYDYARG EVAAVTSCWK EMYREVPCIP CKAPFSSLEE LIDVAVPAAT EREDALKPVQ VFVTGSFFLL SDIMGLLQAH ESKFQTKLKE DSKED
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTA TGAACGCTGC GGTTTGTGGG AGGACGTTTA ACGACGTGGT GCGTCTAATC GAGAGCTGGG CAATAGGAAA GGTGGCCTCC TCTTGCAACA ACCTTGAGAC CACAAGGCTT TTTATAGACC GTCTCAATAT GCAGGGTTAT CTTGACAAGC TTCGTTTTGT CCATGTGGCG GGAACAAAGG GAAAGGGGAC AACCGCGGCG TACACCGCGG CGCTCCTGCA GGCGTATGGG CTTAGGGTTG GCCTTTTCAC CTCCCCACAC CTTGTGGATG TCCGGGAGCG CATTTTGGTG AATAATGCCT TTTTGCCCAA GGATGTATTC GCGAGGCAAT TTTTCAAATT GGTTGACCGC ATTGCGGATC TTTCAAACTC GGAGAGCCAA ATTTCCCGCG ATCTCTCGAA ACGCACAAGC TTCTTTCGTT TCCTGTTTTT ACTCTCGCTG TACATCTTCG CTGAGGAGTT GGTGGATGTG GTGGTTGTGG AGGTGGGCGT GGGGGGCCGC ATCGACGCCA CAAATATCAT TTCGCCCGAG GTGACTGTCA TAACTTCACT GGGTCTCGAT CACACAGAAG TTTTGGGAAA CACCATAGAG GAAATTGCCC TTGAAAAGGC CGGTATTATG AAGCCCGGTG TTATTTGCTA CACCTCACCG CAGGAGTGGC ACCCGTCCAC GCTGACTGTG ATGGAGAAAC ATGCTCGTGG CCAGCAGACT CCCTTTGTGG TTGTGGACGA GAAGACGCTG CCCATATTCA ATTGGCCTCC CCTGGCTATT GGGGGAAACC ACGTCATTGA GGACTCCAAA CTGGCGCTGC TGGCGGCCAG GTATATGGCA GGAATTCCCC CTACCCTGCC TCTGAGTGGC GCTGAACGTC AAGTGTTGCA GACGATGACT CTTCCAGGCC GTTCGCAGGT GCTGTCGATC CACGGCGGAA CGGGCGCAAC GCTTTACCTT GACTGCGCCC ACACGTATGA GAGTTTGACG TGTGCCACCC GCTGGTTTAT GGAGGAAAGC CGTTCCTTAA CTGGAGATGC GAATCCACGT CGCGTGCTTG TTTTTTACTC CACTCGGGAT GCGACGCATG TCCTTAAGTC GTTTATGCCT TTTATTCACT GTTTTTCCAA GATGATTGCC GCATTTGTGG TGAATCCACG GAAGGCGACT GGAAACACGG CGGATGGAAG GTACGATTAT GCGCGAGGCG AAGTAGCAGC CGTGACGAGT TGCTGGAAAG AGATGTATCG TGAGGTTCCA TGTATTCCCT GTAAAGCCCC GTTTTCCTCG CTGGAAGAAC TGATTGATGT GGCCGTTCCC GCTGCAACCG AACGCGAGGA TGCCTTGAAA CCGGTGCAGG TCTTTGTTAC CGGGTCCTTC TTTTTGCTTT CAGACATCAT GGGGCTGCTC CAGGCCCACG AATCCAAATT TCAAACCAAA TTGAAGGAAG ATAGTAAGGA GGACTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT