TrcrA.18246.a

folylpolyglutamate synthetase

CENTER ID: TrcrA.18246.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18246.a.B1.GE35418 full length 1 487
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053510105.80
RefSeq: XP_819886.1
UniProt: Q4DZU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSMNAAVCGR TFNDVVRLIE SWAIGKVASS YNNLETTRLF IDRLNMQGYL DKLRFVHVAG TKGKGTTSAY TAALLQAYGL RVGLFTSPHL VDVRERILVN NAFLPKDVFA RQFFKLLDRI ADLSNSESQI SRDLSKRTSF FRFMFLLSLY IFAEELVDVV VVEVGVGGRI DATNIISPEV TVITSLGLDH TEVLGNTIEE IALEKAGIMK PGVICYTSPQ EWHPSTLTVM ERYARGQQTP FVVVDEKTLP IFNWPPLAIG GNHVIEDSKL ALLAARYMAG IPPTLPLSSA ERQVLQTMTL PGRSQVLSIN GGTGATLYLD CAHTYESLTC ATRWFMEESR SLTGDANPRR VLVFYSTRDA THVLKSFMPF IHCFSKMIAA FVVNPRKATG NTADGRYDYA RGEIAAVTSC WKEMYREVPC IPCKAPFSSL EELIDMAVPA ATEREDALKP VQIFVTGSFF LLSEIMGLLQ AHESKFQTKL KEDSKEG
NT Sequence
atgagtatga acgctgcggt ttgtgggagg acgtttaacg acgtggtgcg tctaatcgag agctgggcaa taggaaaggt ggcctcctct tacaacaacc ttgagaccac aaggcttttt atagaccgtc tcaatatgca gggttatctt gacaagcttc gttttgtcca tgtggcggga acaaagggaa aggggacaac ttcggcgtac accgcggcgc tcctgcaggc gtatgggctt agggttggtc ttttcacctc cccacacctt gtggatgtcc gggagcgcat tttggtgaat aatgcctttt tacccaagga tgtattcgcg aggcaatttt tcaaattgct tgaccgcatt gcggatcttt caaactcgga gagccaaatt tcccgcgatc tctcaaaacg cacaagcttc tttcgtttca tgtttttact ctcgctgtac atcttcgctg aggagttggt ggatgtggtg gttgtggagg tgggcgtggg gggccgcatc gacgccacaa atatcatttc acccgaggtg actgtcataa cgtcactggg tctcgatcac acagaagttt tgggaaatac catagaagaa attgcccttg aaaaggccgg tattatgaag cccggtgtta tttgctacac ctcaccgcag gagtggcacc cgtccacgct gactgtgatg gagagatatg ctcgtggcca gcagactccc tttgtggttg tggacgagaa gacgctgccc atattcaatt ggcctcccct ggctattggg ggaaaccacg tcattgagga ctccaaactg gcgctgctgg cggccagata tatggcagga atccccccta ccctgcctct gagtagcgct gaacgtcaag ttttgcagac gatgactctt ccaggccgtt cgcaggtgct gtcgatcaac ggcggaacgg gcgcaacgct ttaccttgac tgcgcccaca cgtatgagag tttgacgtgt gccacccgct ggtttatgga ggaaagccgt tccttaactg gagatgcgaa tccacgtcgc gtgcttgttt tttactccac tcgggatgcg acgcatgtcc ttaagtcgtt tatgcctttt attcactgtt tttccaagat gattgccgca tttgtggtga atccacggaa ggcgactgga aacacggcgg atggaaggta cgattatgcg cgaggcgaaa tagcagccgt gacgagttgc tggaaagaga tgtatcgtga ggttccatgt attccctgta aagctccgtt ttcctcgctg gaagaactga ttgatatggc cgttcccgct gcaaccgaac gcgaggatgc cttgaaacca gtgcagatct ttgttaccgg gtccttcttt ttgctttcag agatcatggg gctgctccag gcccacgaat ccaaatttca aaccaaattg aaggaagata gtaaggaggg ttag
Details for TrcrA.18246.a.B1.GE35418
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 55kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 93
PERCENT COVERAGE: 96
Validated AA Sequence
MSMNAAVCGR TFNDVVRLIE SWAIGKVASS YNNLETTRLF IDRLNMQGYL DKLRFVHVAG TKGKGTTSAY TAALLQAYGL RVGLFTSPHL VDVRERILVN NAFLPKDVFA RQFFKLLDRI ADLSNSESQI SRDLSKRTXF FRFMFLLSLY IFAEELVDVV VVEVGVGGRX XATNIISPEV TVITSLGLDH TXXLGNTIXE IALEKAGIMK PRCYLLHLTA GVAPVHADCD GEIXSWPADS LCGCGREDAX PXFNWPPLAI GGNHVIEDSK LALLAARYMA GIPPTLPLSS AERQXXQTMT LPXRSQVLSI NGGTGATLYL XCAHTYESLT CATRWFMEES RSLTGDXNPR RVLVFYSTRD ATHVLKSFMP FIHCFSKMIA AFVVNPRKAT GNTADGRYDY ARGEIAAVTS CWKEMYREVP CIPCKAPFSS LEELIDMAVP AATEREDALK PVQIFVTGSF FLLSEIMGLL QAHESKFQT
Validated NT Sequence
tttggtttga aatttggatt cgtgggcctg gagcagcccc atgatctctg aaagcaaaaa gaaggacccg gtaacaaaga tctgcactgg tttcaaggca tcctcgcgtt cggttgcagc gggaacggcc atatcaatca gttcttccag cgaggaaaac ggagctttac agggaataca tggaacctca cgatacatct ctttccagca actcgtcacg gctgctattt cgcctcgcgc ataatcgtac cttccatccg ccgtgtttcc agtcgccttc cgtggattca ccacaaatgc ggcaatcatc ttggaaaaac agtgaataaa aggcataaac gacttaagga catgcgtcgc atcccgagtg gagtaaaaaa caagcacgcg acgtggattc ncatctccag ttaangaacg gctttcctcc ataaaccanc gggtggcaca cgtcaaactc tcatacgtgt gggcgcantc aaggtaaagc gttgcgcccg ttccgccgtt gatcgacagc acctgcgaac ggnctggaag agtcatcgtc tgcnnnnctt gacgttcagc gctactcaga ggcagggtag gggggattcc tgccatatat ctggccgcca gcagcgccag tttggagtcc tcaatgacgt ggtttccccc aatagccagg ggaggccaat tgaantatgg gnnagcgtct tctcgtccac aaccacaaag ggagtctgct ggccacganc atatctctcc atcacagtca gcgtggacgg gtgccactcc tgcggtgagg tgtagcaaat aacaccgggg cttcataata ccggcctttt caagggcaat ttcttntatg gtatttccca aaanntctgt gtgatcgaga cccagtgacg ttatgacagt cacctcgggt gaaatgatat ttgtggcgtn gnngcggccc cccacgccca cctccacaac caccacatcc accaactcct cagcgaagat gtacagcgag agtaaaaaca tgaaacgaaa gaagnttgtg cgttttgaga gatcgcggga aatttggctc tccgagtttg aaagatccgc aatgcggtca agcaatttga aaaattgcct cgcgaataca tccttgggta aaaaggcatt attcaccaaa atgcgctccc ggacatccac aaggtgtggg gaggtgaaaa gaccaaccct aagcccatac gcctgcagga gcgccgcggt gtacgccgaa gttgtcccct ttccctttgt tcccgccaca tggacaaaac gaagcttgtc aagataaccc tgcatattga gacggtctat aaaaagcctt gtggtctcaa ggttgttgta agaggaggcc acctttccta ttgcccagct ctcgattaga cgcaccacgt cgttaaacgt cctcccacaa accgcagcgt tcatactcat
Expected Protein Sequence
MAHHHHHHMS MNAAVCGRTF NDVVRLIESW AIGKVASSYN NLETTRLFID RLNMQGYLDK LRFVHVAGTK GKGTTSAYTA ALLQAYGLRV GLFTSPHLVD VRERILVNNA FLPKDVFARQ FFKLLDRIAD LSNSESQISR DLSKRTSFFR FMFLLSLYIF AEELVDVVVV EVGVGGRIDA TNIISPEVTV ITSLGLDHTE VLGNTIEEIA LEKAGIMKPG VICYTSPQEW HPSTLTVMER YARGQQTPFV VVDEKTLPIF NWPPLAIGGN HVIEDSKLAL LAARYMAGIP PTLPLSSAER QVLQTMTLPG RSQVLSINGG TGATLYLDCA HTYESLTCAT RWFMEESRSL TGDANPRRVL VFYSTRDATH VLKSFMPFIH CFSKMIAAFV VNPRKATGNT ADGRYDYARG EIAAVTSCWK EMYREVPCIP CKAPFSSLEE LIDMAVPAAT EREDALKPVQ IFVTGSFFLL SEIMGLLQAH ESKFQTKLKE DSKEG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta tgaacgctgc ggtttgtggg aggacgttta acgacgtggt gcgtctaatc gagagctggg caataggaaa ggtggcctcc tcttacaaca accttgagac cacaaggctt tttatagacc gtctcaatat gcagggttat cttgacaagc ttcgttttgt ccatgtggcg ggaacaaagg gaaaggggac aacttcggcg tacaccgcgg cgctcctgca ggcgtatggg cttagggttg gtcttttcac ctccccacac cttgtggatg tccgggagcg cattttggtg aataatgcct ttttacccaa ggatgtattc gcgaggcaat ttttcaaatt gcttgaccgc attgcggatc tttcaaactc ggagagccaa atttcccgcg atctctcaaa acgcacaagc ttctttcgtt tcatgttttt actctcgctg tacatcttcg ctgaggagtt ggtggatgtg gtggttgtgg aggtgggcgt ggggggccgc atcgacgcca caaatatcat ttcacccgag gtgactgtca taacgtcact gggtctcgat cacacagaag ttttgggaaa taccatagaa gaaattgccc ttgaaaaggc cggtattatg aagcccggtg ttatttgcta cacctcaccg caggagtggc acccgtccac gctgactgtg atggagagat atgctcgtgg ccagcagact ccctttgtgg ttgtggacga gaagacgctg cccatattca attggcctcc cctggctatt gggggaaacc acgtcattga ggactccaaa ctggcgctgc tggcggccag atatatggca ggaatccccc ctaccctgcc tctgagtagc gctgaacgtc aagttttgca gacgatgact cttccaggcc gttcgcaggt gctgtcgatc aacggcggaa cgggcgcaac gctttacctt gactgcgccc acacgtatga gagtttgacg tgtgccaccc gctggtttat ggaggaaagc cgttccttaa ctggagatgc gaatccacgt cgcgtgcttg ttttttactc cactcgggat gcgacgcatg tccttaagtc gtttatgcct tttattcact gtttttccaa gatgattgcc gcatttgtgg tgaatccacg gaaggcgact ggaaacacgg cggatggaag gtacgattat gcgcgaggcg aaatagcagc cgtgacgagt tgctggaaag agatgtatcg tgaggttcca tgtattccct gtaaagctcc gttttcctcg ctggaagaac tgattgatat ggccgttccc gctgcaaccg aacgcgagga tgccttgaaa ccagtgcaga tctttgttac cgggtccttc tttttgcttt cagagatcat ggggctgctc caggcccacg aatccaaatt tcaaaccaaa ttgaaggaag atagtaagga gggttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat