TrcrA.18244.b

ascorbate-dependent peroxidase, putative

CENTER ID: TrcrA.18244.b
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18244.b.B2.GE35427 mature protein 15 328
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053506193.60
RefSeq: XP_808649.1
UniProt: Q4D2P4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVFCFGSFFS KYASSKSGFQ ARYRFLHSSA KIAAGATGAL LLGGATVALC YFPSGQKVTE APPFDVNSLR RDIEEILSED MSKGPLFVRL AWHEAGSWDC RKKDGSPNSA SMRFHPECSY AGNKGLDKGR NALESLKKKY PKISYADLWS FAAVVSIEAM GGPEIPWRWG RVDAKDGSVC GPDGRLPDAS RMQDHVRDVF SRLGFNDEET VALIGAHTCG ECHLENTGYV GPWTHDKYGF DNSFFTELFG NEWMLNPNVK KMQFMDKTTN RLMMLPADVS ILLDDKYRSI AKKYADDNDY FCNAFSKAYQ KLLEVGTTNL KSLPAESK
NT Sequence
atggtttttt gttttggttc attcttttcg aagtacgctt catccaaatc aggttttcag gcaaggtacc gttttcttca ttcctcggct aaaattgctg ctggcgccac cggtgcattg cttttgggcg gtgccaccgt ggccttatgt tattttccct cgggacaaaa ggtcacggag gcacccccgt ttgatgtgaa ctcactaaga agggacattg aagaaatact ctccgaggac atgtctaaag gacctctttt tgtgcgactc gcgtggcacg aggctggatc atgggactgc cggaaaaagg atgggagtcc taattccgct tcgatgcgat ttcacccaga gtgcagctat gccggcaaca aagggcttga taaaggacga aatgcgctgg aatcgctcaa gaaaaagtac ccgaaaattt catacgccga tttatggtcc ttcgcagcgg tggtgtcgat tgaggcgatg ggcggcccgg agattccgtg gcggtggggg cgcgtggatg caaaggacgg cagcgtttgt ggccccgatg ggcgtctccc tgacgcatcc aggatgcagg atcacgtgcg ggatgtcttt tcacgtcttg gctttaatga tgaagaaact gttgccttga tcggggccca cacatgtggt gagtgccatc tggagaacac gggctacgtg ggtccgtgga cgcacgacaa gtacggcttt gacaactcct tttttacgga gctttttggc aacgagtgga tgctgaaccc caatgtgaag aaaatgcagt tcatggacaa aaccactaat cgccttatga tgctgcccgc tgatgtttcc attcttctgg acgacaaata tcgctccatc gcgaagaagt acgcggacga caatgattat ttttgcaacg ccttcagcaa ggcttatcag aagttgcttg aggttggaac caccaatctc aagtctctcc cagcagagtc aaaatag
Details for TrcrA.18244.b.B2.GE35427
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMS KSGFQARYRF LHSSAKIAAG ATGALLLGGA TVALCYFPSG RKVTEAPPFD VNSLRRDIEE ILSEDMSKGP LFVRLAWHEA GSWDCRKKDG SPNSASMRFH PECSYAGNKG LDKGRNALES LKKKYPKISY ADLWSFAAVV SIEAMGGPEI PWRWGRVDAK DGSVCGPDGR LPDASRMQDH VRDVFSRLGF NDEETVALIG AHTCGECHLE NTGYVGPWTH DKYGFDNSFF TELFGNEWML NPNVKKMQFM DKTTNRLMML PADVSILLDD KYRSIAKKYA DXXIIFXXXS XSXXXXXXSQ XXXXXQIXXX XX
Validated NT Sequence
tttgtttact ttaagaagga ganataccat ggctcaccac caccaccacc atatgtccaa atcaggtttt caggcaaggt accgttttct tcattcctcg gctaaaattg ctgccggcgc caccggtgca ttgcttttgg gcggtgccac cgtggcctta tgttattttc cctcgggacg aaaggtcacg gaggcaccgc cgtttgatgt gaactcacta agaagggaca ttgaagaaat actctccgag gatatgtcta aagggcctct ttttgtgcga ctcgcgtggc acgaggctgg atcatgggac tgccggaaaa aggatgggag tcctaattcc gcttcgatgc gatttcaccc agagtgcagc tatgccggca acaaagggct tgataaagga cgaaatgcgc tggaatcgct caagaaaaag tacccgaaaa tttcatacgc cgatttatgg tccttcgcag cggtggtgtc gattgaggcg atgggtggcc cggagattcc gtggcggtgg gggcgcgtgg atgcaaagga cggcagcgtt tgtggccccg atgggcgtct ccctgacgcg tccaggatgc aggatcacgt gcgggatgtc ttttcacgtc ttggctttaa tgatgaagaa actgttgcct tgatcggggc ccacacatgt ggtgagtgcc atctggagaa cacgggctac gtgggtccgt ggacgcacga caagtatggc tttgacaact ccttttttac ggagcttttt ggcaacgagt ggatgctgaa ccccaatgtg aagaaaatgc agttcatgga caaaaccact aaccgcctta tgatgctgcc cgctgatgtt tccattcttc tggacgacaa gtatcgttcc atcgcgaaga agtacgcgga cgannngatt atttttnnan ncntcagcnt ntcnnantnn tgnnngannc natctcagtn nntccnncnn antcaaatan tgnnnanann nnancg
Expected Protein Sequence
MAHHHHHHMS KSGFQARYRF LHSSAKIAAG ATGALLLGGA TVALCYFPSG QKVTEAPPFD VNSLRRDIEE ILSEDMSKGP LFVRLAWHEA GSWDCRKKDG SPNSASMRFH PECSYAGNKG LDKGRNALES LKKKYPKISY ADLWSFAAVV SIEAMGGPEI PWRWGRVDAK DGSVCGPDGR LPDASRMQDH VRDVFSRLGF NDEETVALIG AHTCGECHLE NTGYVGPWTH DKYGFDNSFF TELFGNEWML NPNVKKMQFM DKTTNRLMML PADVSILLDD KYRSIAKKYA DDNDYFCNAF SKAYQKLLEV GTTNLKSLPA ESK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca aatcaggttt tcaggcaagg taccgttttc ttcattcctc ggctaaaatt gctgctggcg ccaccggtgc attgcttttg ggcggtgcca ccgtggcctt atgttatttt ccctcgggac aaaaggtcac ggaggcaccc ccgtttgatg tgaactcact aagaagggac attgaagaaa tactctccga ggacatgtct aaaggacctc tttttgtgcg actcgcgtgg cacgaggctg gatcatggga ctgccggaaa aaggatggga gtcctaattc cgcttcgatg cgatttcacc cagagtgcag ctatgccggc aacaaagggc ttgataaagg acgaaatgcg ctggaatcgc tcaagaaaaa gtacccgaaa atttcatacg ccgatttatg gtccttcgca gcggtggtgt cgattgaggc gatgggcggc ccggagattc cgtggcggtg ggggcgcgtg gatgcaaagg acggcagcgt ttgtggcccc gatgggcgtc tccctgacgc atccaggatg caggatcacg tgcgggatgt cttttcacgt cttggcttta atgatgaaga aactgttgcc ttgatcgggg cccacacatg tggtgagtgc catctggaga acacgggcta cgtgggtccg tggacgcacg acaagtacgg ctttgacaac tcctttttta cggagctttt tggcaacgag tggatgctga accccaatgt gaagaaaatg cagttcatgg acaaaaccac taatcgcctt atgatgctgc ccgctgatgt ttccattctt ctggacgaca aatatcgctc catcgcgaag aagtacgcgg acgacaatga ttatttttgc aacgccttca gcaaggctta tcagaagttg cttgaggttg gaaccaccaa tctcaagtct ctcccagcag agtcaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat