TrcrA.18244.a

ascorbate-dependent peroxidase, putative

CENTER ID: TrcrA.18244.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18244.a.B2.GE35237 mature protein 50 328
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18244.a.B2.PS01527 mature protein 50 328
TrcrA.18244.a.B2.PS01528 mature protein 50 328

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053503745.30
RefSeq: XP_804882.1
UniProt: Q8I1N3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAFCFGSFFS KYASSKSGSQ ARYRFLHSSA KIAAGATGAL LLGGATVALC YFPSGRKVTE APPFDVNSLR RDIEEILSED MSKGPLFVRL AWHEAGSWDC RKKDGSPNSA SMRFHPECSY AGNKGLDKGR NALESLKKKY PKISYADLWS FAAVVSIEAM GGPEIPWRWG RVDAKDGSVC GPDGRLPDAS RMQDHVRDVF SRLGFNDEET VALIGAHTCG ECHLENTGYV GPWTHDKYGF DNSFFTELFG NEWMLNPNVK KMQFMDKTTN RLMMLPADVS ILLDDKYRSI AKKYADDNDY FCNAFSKAYQ KLLEVGTTDL KSLPAESK
NT Sequence
atggcttttt gttttggttc attcttttcg aagtacgctt catccaaatc aggttctcag gcaaggtacc gttttcttca ttcctcggct aaaattgctg ccggcgccac cggtgcattg cttttgggcg gtgccaccgt ggccttatgt tattttccct cgggacgaaa ggtcacggag gcaccgccgt ttgatgtgaa ctcactaaga agggacattg aagaaatact ctccgaggat atgtctaaag ggcctctttt tgtgcgactc gcgtggcacg aggctggatc atgggactgc cggaaaaagg atgggagtcc taattccgct tcgatgcgat ttcacccaga gtgcagctat gccggcaaca aagggcttga taaaggacga aatgcgctgg aatcgctcaa gaaaaagtac ccgaaaattt catacgccga tttatggtcc ttcgcagcgg tggtgtcgat tgaggcgatg ggtggcccgg agattccgtg gcggtggggg cgcgtggatg caaaggacgg cagcgtttgt ggccccgatg ggcgtctccc tgacgcgtcc aggatgcagg atcacgtgcg ggatgtcttt tcacgtcttg gctttaatga tgaagaaact gttgccttga tcggggccca cacatgtggt gagtgccatc tggagaacac gggctacgtg ggtccgtgga cgcacgacaa gtacggcttt gacaactcct tttttacgga gctttttggc aacgagtgga tgctgaaccc caatgtgaag aaaatgcagt tcatggacaa aaccactaac cgccttatga tgctgcccgc tgatgtttcc attcttctgg acgacaagta tcgttccatc gcgaagaagt acgcggacga caatgattat ttttgcaacg ccttcagcaa ggcttatcag aagttgcttg aggttggaac caccgatctc aagtctctcc cagcagagtc aaaatag
Details for TrcrA.18244.a.B2.GE35237
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMC YFPSGQKVTE APPFDVNSLR RDIEEILSED MSKGPLFVRL AWHEAGSWDC RKKDGSPNSA SMRFHPECSY AGNKGLDKGR NALESLKKKY PKISYADLWS FAAVVSIEAM GGPEIPWRWG RVDAKDGSVC GPDGRLPDAS RMQDHVRDVF SRLGFNDEET VALIGAHTCG ECHLENTGYV GPWTHDKYGF DNSFFTELFG NEWMLNPNVK KMQFMDKTTN RLMMLPADVS ILLDDKYRSI AXKYADDNDY FCNAFSKAYQ XLLXVGTTNL KSLPAXSK
Validated NT Sequence
ttactcantt attttgantc tgctgggaga gacttgagat tggtggttcc aacntcaagc aanttctgat aagccttgct gaaggcgttg caaaaataat cattgtcgtc cgcgtacttn ttcgcgatgg agcgatattt gtcgtccaga agaatggaaa catcagcggg cagcatcata aggcgattag tggttttgtc catgaactgc attttcttca cattggggtt cagcatccac tcgttgccaa aaagctccgt aaaaaaggag ttgtcaaagc cgtacttgtc gtgcgtccac ggacccacgt agcccgtgtt ctccagatgg cactcaccac atgtgtgggc cccgatcaag gcaacagttt cttcatcatt aaagccaaga cgtgaaaaga catcccgcac gtgatcctgc atcctggatg cgtcagggag acgcccatcg gggccacaaa cgctgccgtc ctttgcatcc acgcgccccc accgccacgg aatctccggg ccgcccatcg cctcaatcga caccaccgct gcgaaggacc ataaatcggc gtatgaaatt ttcgggtact ttttcttgag cgattccagc gcatttcgtc ctttatcaag ccctttgttg ccggcatagc tgcactctgg gtgaaatcgc atcgaagcgg aattaggact cccatccttt ttccggcagt cccatgatcc agcctcgtgc cacgcgagtc gcacaaaaag aggtccttta gacatgtcct cggagagtat ttcttcaatg tcccttctta gtgagttcac atcaaacggg ggtgcctccg tgaccttttg tcccgaggga aaataacaca tatggtggtg gtggtggtga gccatggtat
Expected Protein Sequence
MAHHHHHHMC YFPSGRKVTE APPFDVNSLR RDIEEILSED MSKGPLFVRL AWHEAGSWDC RKKDGSPNSA SMRFHPECSY AGNKGLDKGR NALESLKKKY PKISYADLWS FAAVVSIEAM GGPEIPWRWG RVDAKDGSVC GPDGRLPDAS RMQDHVRDVF SRLGFNDEET VALIGAHTCG ECHLENTGYV GPWTHDKYGF DNSFFTELFG NEWMLNPNVK KMQFMDKTTN RLMMLPADVS ILLDDKYRSI AKKYADDNDY FCNAFSKAYQ KLLEVGTTDL KSLPAESK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgtt attttccctc gggacgaaag gtcacggagg caccgccgtt tgatgtgaac tcactaagaa gggacattga agaaatactc tccgaggata tgtctaaagg gcctcttttt gtgcgactcg cgtggcacga ggctggatca tgggactgcc ggaaaaagga tgggagtcct aattccgctt cgatgcgatt tcacccagag tgcagctatg ccggcaacaa agggcttgat aaaggacgaa atgcgctgga atcgctcaag aaaaagtacc cgaaaatttc atacgccgat ttatggtcct tcgcagcggt ggtgtcgatt gaggcgatgg gtggcccgga gattccgtgg cggtgggggc gcgtggatgc aaaggacggc agcgtttgtg gccccgatgg gcgtctccct gacgcgtcca ggatgcagga tcacgtgcgg gatgtctttt cacgtcttgg ctttaatgat gaagaaactg ttgccttgat cggggcccac acatgtggtg agtgccatct ggagaacacg ggctacgtgg gtccgtggac gcacgacaag tacggctttg acaactcctt ttttacggag ctttttggca acgagtggat gctgaacccc aatgtgaaga aaatgcagtt catggacaaa accactaacc gccttatgat gctgcccgct gatgtttcca ttcttctgga cgacaagtat cgttccatcg cgaagaagta cgcggacgac aatgattatt tttgcaacgc cttcagcaag gcttatcaga agttgcttga ggttggaacc accgatctca agtctctccc agcagagtca aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for TrcrA.18244.a.B2.PS01527
PURIFICATION DATe: 6/14/2012
CONCENTRATION: 38.99mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMC YFPSGQKVTE APPFDVNSLR RDIEEILSED MSKGPLFVRL AWHEAGSWDC RKKDGSPNSA SMRFHPECSY AGNKGLDKGR NALESLKKKY PKISYADLWS FAAVVSIEAM GGPEIPWRWG RVDAKDGSVC GPDGRLPDAS RMQDHVRDVF SRLGFNDEET VALIGAHTCG ECHLENTGYV GPWTHDKYGF DNSFFTELFG NEWMLNPNVK KMQFMDKTTN RLMMLPADVS ILLDDKYRSI AXKYADDNDY FCNAFSKAYQ XLLXVGTTNL KSLPAXSK
Validated NT Sequence
ttactcantt attttgantc tgctgggaga gacttgagat tggtggttcc aacntcaagc aanttctgat aagccttgct gaaggcgttg caaaaataat cattgtcgtc cgcgtacttn ttcgcgatgg agcgatattt gtcgtccaga agaatggaaa catcagcggg cagcatcata aggcgattag tggttttgtc catgaactgc attttcttca cattggggtt cagcatccac tcgttgccaa aaagctccgt aaaaaaggag ttgtcaaagc cgtacttgtc gtgcgtccac ggacccacgt agcccgtgtt ctccagatgg cactcaccac atgtgtgggc cccgatcaag gcaacagttt cttcatcatt aaagccaaga cgtgaaaaga catcccgcac gtgatcctgc atcctggatg cgtcagggag acgcccatcg gggccacaaa cgctgccgtc ctttgcatcc acgcgccccc accgccacgg aatctccggg ccgcccatcg cctcaatcga caccaccgct gcgaaggacc ataaatcggc gtatgaaatt ttcgggtact ttttcttgag cgattccagc gcatttcgtc ctttatcaag ccctttgttg ccggcatagc tgcactctgg gtgaaatcgc atcgaagcgg aattaggact cccatccttt ttccggcagt cccatgatcc agcctcgtgc cacgcgagtc gcacaaaaag aggtccttta gacatgtcct cggagagtat ttcttcaatg tcccttctta gtgagttcac atcaaacggg ggtgcctccg tgaccttttg tcccgaggga aaataacaca tatggtggtg gtggtggtga gccatggtat
Expressed Protein Sequence
MAHHHHHHMC YFPSGRKVTE APPFDVNSLR RDIEEILSED MSKGPLFVRL AWHEAGSWDC RKKDGSPNSA SMRFHPECSY AGNKGLDKGR NALESLKKKY PKISYADLWS FAAVVSIEAM GGPEIPWRWG RVDAKDGSVC GPDGRLPDAS RMQDHVRDVF SRLGFNDEET VALIGAHTCG ECHLENTGYV GPWTHDKYGF DNSFFTELFG NEWMLNPNVK KMQFMDKTTN RLMMLPADVS ILLDDKYRSI AKKYADDNDY FCNAFSKAYQ KLLEVGTTDL KSLPAESK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTGTT ATTTTCCCTC GGGACGAAAG GTCACGGAGG CACCGCCGTT TGATGTGAAC TCACTAAGAA GGGACATTGA AGAAATACTC TCCGAGGATA TGTCTAAAGG GCCTCTTTTT GTGCGACTCG CGTGGCACGA GGCTGGATCA TGGGACTGCC GGAAAAAGGA TGGGAGTCCT AATTCCGCTT CGATGCGATT TCACCCAGAG TGCAGCTATG CCGGCAACAA AGGGCTTGAT AAAGGACGAA ATGCGCTGGA ATCGCTCAAG AAAAAGTACC CGAAAATTTC ATACGCCGAT TTATGGTCCT TCGCAGCGGT GGTGTCGATT GAGGCGATGG GTGGCCCGGA GATTCCGTGG CGGTGGGGGC GCGTGGATGC AAAGGACGGC AGCGTTTGTG GCCCCGATGG GCGTCTCCCT GACGCGTCCA GGATGCAGGA TCACGTGCGG GATGTCTTTT CACGTCTTGG CTTTAATGAT GAAGAAACTG TTGCCTTGAT CGGGGCCCAC ACATGTGGTG AGTGCCATCT GGAGAACACG GGCTACGTGG GTCCGTGGAC GCACGACAAG TACGGCTTTG ACAACTCCTT TTTTACGGAG CTTTTTGGCA ACGAGTGGAT GCTGAACCCC AATGTGAAGA AAATGCAGTT CATGGACAAA ACCACTAACC GCCTTATGAT GCTGCCCGCT GATGTTTCCA TTCTTCTGGA CGACAAGTAT CGTTCCATCG CGAAGAAGTA CGCGGACGAC AATGATTATT TTTGCAACGC CTTCAGCAAG GCTTATCAGA AGTTGCTTGA GGTTGGAACC ACCGATCTCA AGTCTCTCCC AGCAGAGTCA AAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for TrcrA.18244.a.B2.PS01528
PURIFICATION DATe: 6/14/2012
CONCENTRATION: 33.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMC YFPSGQKVTE APPFDVNSLR RDIEEILSED MSKGPLFVRL AWHEAGSWDC RKKDGSPNSA SMRFHPECSY AGNKGLDKGR NALESLKKKY PKISYADLWS FAAVVSIEAM GGPEIPWRWG RVDAKDGSVC GPDGRLPDAS RMQDHVRDVF SRLGFNDEET VALIGAHTCG ECHLENTGYV GPWTHDKYGF DNSFFTELFG NEWMLNPNVK KMQFMDKTTN RLMMLPADVS ILLDDKYRSI AXKYADDNDY FCNAFSKAYQ XLLXVGTTNL KSLPAXSK
Validated NT Sequence
ttactcantt attttgantc tgctgggaga gacttgagat tggtggttcc aacntcaagc aanttctgat aagccttgct gaaggcgttg caaaaataat cattgtcgtc cgcgtacttn ttcgcgatgg agcgatattt gtcgtccaga agaatggaaa catcagcggg cagcatcata aggcgattag tggttttgtc catgaactgc attttcttca cattggggtt cagcatccac tcgttgccaa aaagctccgt aaaaaaggag ttgtcaaagc cgtacttgtc gtgcgtccac ggacccacgt agcccgtgtt ctccagatgg cactcaccac atgtgtgggc cccgatcaag gcaacagttt cttcatcatt aaagccaaga cgtgaaaaga catcccgcac gtgatcctgc atcctggatg cgtcagggag acgcccatcg gggccacaaa cgctgccgtc ctttgcatcc acgcgccccc accgccacgg aatctccggg ccgcccatcg cctcaatcga caccaccgct gcgaaggacc ataaatcggc gtatgaaatt ttcgggtact ttttcttgag cgattccagc gcatttcgtc ctttatcaag ccctttgttg ccggcatagc tgcactctgg gtgaaatcgc atcgaagcgg aattaggact cccatccttt ttccggcagt cccatgatcc agcctcgtgc cacgcgagtc gcacaaaaag aggtccttta gacatgtcct cggagagtat ttcttcaatg tcccttctta gtgagttcac atcaaacggg ggtgcctccg tgaccttttg tcccgaggga aaataacaca tatggtggtg gtggtggtga gccatggtat
Expressed Protein Sequence
MAHHHHHHMC YFPSGRKVTE APPFDVNSLR RDIEEILSED MSKGPLFVRL AWHEAGSWDC RKKDGSPNSA SMRFHPECSY AGNKGLDKGR NALESLKKKY PKISYADLWS FAAVVSIEAM GGPEIPWRWG RVDAKDGSVC GPDGRLPDAS RMQDHVRDVF SRLGFNDEET VALIGAHTCG ECHLENTGYV GPWTHDKYGF DNSFFTELFG NEWMLNPNVK KMQFMDKTTN RLMMLPADVS ILLDDKYRSI AKKYADDNDY FCNAFSKAYQ KLLEVGTTDL KSLPAESK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTGTT ATTTTCCCTC GGGACGAAAG GTCACGGAGG CACCGCCGTT TGATGTGAAC TCACTAAGAA GGGACATTGA AGAAATACTC TCCGAGGATA TGTCTAAAGG GCCTCTTTTT GTGCGACTCG CGTGGCACGA GGCTGGATCA TGGGACTGCC GGAAAAAGGA TGGGAGTCCT AATTCCGCTT CGATGCGATT TCACCCAGAG TGCAGCTATG CCGGCAACAA AGGGCTTGAT AAAGGACGAA ATGCGCTGGA ATCGCTCAAG AAAAAGTACC CGAAAATTTC ATACGCCGAT TTATGGTCCT TCGCAGCGGT GGTGTCGATT GAGGCGATGG GTGGCCCGGA GATTCCGTGG CGGTGGGGGC GCGTGGATGC AAAGGACGGC AGCGTTTGTG GCCCCGATGG GCGTCTCCCT GACGCGTCCA GGATGCAGGA TCACGTGCGG GATGTCTTTT CACGTCTTGG CTTTAATGAT GAAGAAACTG TTGCCTTGAT CGGGGCCCAC ACATGTGGTG AGTGCCATCT GGAGAACACG GGCTACGTGG GTCCGTGGAC GCACGACAAG TACGGCTTTG ACAACTCCTT TTTTACGGAG CTTTTTGGCA ACGAGTGGAT GCTGAACCCC AATGTGAAGA AAATGCAGTT CATGGACAAA ACCACTAACC GCCTTATGAT GCTGCCCGCT GATGTTTCCA TTCTTCTGGA CGACAAGTAT CGTTCCATCG CGAAGAAGTA CGCGGACGAC AATGATTATT TTTGCAACGC CTTCAGCAAG GCTTATCAGA AGTTGCTTGA GGTTGGAACC ACCGATCTCA AGTCTCTCCC AGCAGAGTCA AAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT