TrcrA.18243.b

S-adenosylmethionine decarboxylase proenzyme

CENTER ID: TrcrA.18243.b
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18243.b.B1.GE35236 full length 1 370
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18243.b.B1.PS01518 full length 1 370

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053509167.120
RefSeq: XP_816711.1
UniProt: Q4DQQ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSNKDPLSL MAMWGSVKGY DPNQGASFEG PEKRLEVIMR ITDETHSEGL HALGDGVWKG VVGSLNAQIV SKESNEYIRS YVLTESSLFV MRDRIILITC GTTTLLNAVP FVLEAVSDVR GEVEWVSFMH KNYSFPWEQK GPHLSMAEEF NTLRTYFPSG KPFIFGPVDS DHYFLFVYDD VIRPCETEND TQLSMTMYGL DRTQTKHWFS DRFISTGTET AAIRKATKLN KVADDSWKLH DLQFEPCGYS INTIRGAEYQ TIHITPEDHC SFASYETNTP AVNYSERINT VLGVFAPIRF SVIVFIDPDS DVGRLYQKGQ NVGVEAEYYP KYELQNRTVN EFAPGYVVMK MNYAMRAEVA EKDSTDPVEE
NT Sequence
atgttaagca ataaggaccc tctctctttg atggcgatgt ggggttcagt gaagggctac gacccaaatc agggggccag ttttgagggg ccggaaaaac gtttggaggt gatcatgcgt attactgacg agacccactc ggagggcctc catgcccttg gagatggggt atggaaaggt gtggtgggct cgttaaatgc ccaaattgta tccaaagaga gtaatgagta tattcgctct tatgtgctaa cggaaagctc attgtttgtc atgcgggacc gtatcatcct gatcacatgt ggcacgacga ctctacttaa tgccgttcct ttcgttttgg aggcagttag tgatgtgcga ggggaggtgg aatgggtctc tttcatgcat aagaactaca gctttccgtg ggaacagaag ggaccacatc tctccatggc agaggaattc aacactttaa gaacgtactt tccatcggga aagcctttta tatttggtcc tgtcgatagt gatcattact ttctattcgt ctatgacgac gttattcgtc catgtgagac ggagaacgat acacagttga gtatgacaat gtacgggcta gatcgtacgc agacgaagca ttggttctcg gatcgtttta tctcaacagg cacagagacg gccgctatcc gcaaggcgac aaaactgaac aaggtggcag atgactcatg gaagttgcat gatttgcaat ttgagccgtg tgggtatagt ataaatacaa ttcgtggtgc agaataccag acgatccaca taacaccgga agatcactgc tcttttgcct cgtacgaaac gaatacaccc gctgtcaatt attcggagcg gataaacacc gtgttgggag tttttgctcc aatacgtttt tccgtgattg tcttcattga tcctgacagt gatgttggaa ggctgtacca gaagggacag aacgttgggg ttgaagcgga gtattaccct aagtatgaat tgcagaaccg tacggtcaat gaatttgccc caggctacgt ggtgatgaag atgaattacg caatgagggc ggaagtagct gagaaagact ccacagatcc cgtggaagag tag
Details for TrcrA.18243.b.B1.GE35236
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 43kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MAMWGSVKGY DPNQGASFEG PEKRLEVIMR ITDETHSEGL HALGDGVWKG VVGSLNAQIV SKESNEYIRS YVLTESSLFV MRDRIILITC GTTTLLNAVP FVLEAVSDVR GEVEWVSFMH KNYSFPWEQK GPHLSMAEEF NTLRTYFPSG KPFIFGPVDS DHYFLFVYDD VIRPCETEND TQLSMTMYGL DRTQTKHWFS DRFISTGTET AAIRKATKLN KVADDSWKLH DLQFEPCGYS INTIRGAEYQ TIHITPEDHC SFASYETNTP AVNYSERINT VLGVFAPIRF SVIVFIDPDS DVGRLYQKGQ NVGVEAEYYP KYELQNRTVN EFAPGYVVMK MNYAMRAEV
Validated NT Sequence
gctacttccg ccctcattgc gtaattcatc ttcatcacca cgtagcctgg ggcaaattca ttgaccgtac ggttctgcaa ttcatactta gggtaatact ccgcttcaac cccaacgttc tgtcccttct ggtacagcct tccaacatca ctgtcaggat caatgaagac aatcacggaa aaacgtattg gagcaaaaac tcccaacacg gtgtttatcc gctccgaata attgacagcg ggtgtattcg tttcgtacga ggcaaaagag cagtgatctt ccggtgttat gtggatcgtc tggtattctg caccacgaat tgtatttata ctatacccac acggctcaaa ttgcaaatca tgcaacttcc atgagtcatc tgccaccttg ttcagttttg tcgccttgcg gatagcggcc gtctctgtgc ctgttgagat aaaacgatcc gagaaccaat gcttcgtctg cgtacgatct agcccgtaca ttgtcatact caactgtgta tcgttctccg tctcacatgg acgaataacg tcgtcataga cgaatagaaa gtaatgatca ctatcgacag gaccaaatat aaaaggcttt cccgatggaa agtacgttct taaagtgttg aattcctctg ccatggagag atgtggtccc ttctgttccc acggaaagct gtagttctta tgcatgaaag agacccattc cacctcccct cgcacatcac taactgcctc caaaacgaaa ggaacggcat taagtagagt cgtcgtgcca catgtgatca ggatgatacg gtcccgcatg acaaacaatg agctttccgt tagcacataa gagcgaatat actcattact ctctttggat acaatttggg catttaacga gcccaccaca cctttccata ccccatctcc aagggcatgg aggccctccg agtgggtctc gtcagtaata cgcatgatca cctccaaacg tttttccggc ccctcaaaac tggccccctg atttgggtcg tagcccttca ctgaacccca catcgccatc aaagagagag ggtccttat
Expected Protein Sequence
MAHHHHHHML SNKDPLSLMA MWGSVKGYDP NQGASFEGPE KRLEVIMRIT DETHSEGLHA LGDGVWKGVV GSLNAQIVSK ESNEYIRSYV LTESSLFVMR DRIILITCGT TTLLNAVPFV LEAVSDVRGE VEWVSFMHKN YSFPWEQKGP HLSMAEEFNT LRTYFPSGKP FIFGPVDSDH YFLFVYDDVI RPCETENDTQ LSMTMYGLDR TQTKHWFSDR FISTGTETAA IRKATKLNKV ADDSWKLHDL QFEPCGYSIN TIRGAEYQTI HITPEDHCSF ASYETNTPAV NYSERINTVL GVFAPIRFSV IVFIDPDSDV GRLYQKGQNV GVEAEYYPKY ELQNRTVNEF APGYVVMKMN YAMRAEVAEK DSTDPVEE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa gcaataagga ccctctctct ttgatggcga tgtggggttc agtgaagggc tacgacccaa atcagggggc cagttttgag gggccggaaa aacgtttgga ggtgatcatg cgtattactg acgagaccca ctcggagggc ctccatgccc ttggagatgg ggtatggaaa ggtgtggtgg gctcgttaaa tgcccaaatt gtatccaaag agagtaatga gtatattcgc tcttatgtgc taacggaaag ctcattgttt gtcatgcggg accgtatcat cctgatcaca tgtggcacga cgactctact taatgccgtt cctttcgttt tggaggcagt tagtgatgtg cgaggggagg tggaatgggt ctctttcatg cataagaact acagctttcc gtgggaacag aagggaccac atctctccat ggcagaggaa ttcaacactt taagaacgta ctttccatcg ggaaagcctt ttatatttgg tcctgtcgat agtgatcatt actttctatt cgtctatgac gacgttattc gtccatgtga gacggagaac gatacacagt tgagtatgac aatgtacggg ctagatcgta cgcagacgaa gcattggttc tcggatcgtt ttatctcaac aggcacagag acggccgcta tccgcaaggc gacaaaactg aacaaggtgg cagatgactc atggaagttg catgatttgc aatttgagcc gtgtgggtat agtataaata caattcgtgg tgcagaatac cagacgatcc acataacacc ggaagatcac tgctcttttg cctcgtacga aacgaataca cccgctgtca attattcgga gcggataaac accgtgttgg gagtttttgc tccaatacgt ttttccgtga ttgtcttcat tgatcctgac agtgatgttg gaaggctgta ccagaaggga cagaacgttg gggttgaagc ggagtattac cctaagtatg aattgcagaa ccgtacggtc aatgaatttg ccccaggcta cgtggtgatg aagatgaatt acgcaatgag ggcggaagta gctgagaaag actccacaga tcccgtggaa gagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for TrcrA.18243.b.B1.PS01518
PURIFICATION DATe: 5/23/2012
CONCENTRATION: 61.41mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MAMWGSVKGY DPNQGASFEG PEKRLEVIMR ITDETHSEGL HALGDGVWKG VVGSLNAQIV SKESNEYIRS YVLTESSLFV MRDRIILITC GTTTLLNAVP FVLEAVSDVR GEVEWVSFMH KNYSFPWEQK GPHLSMAEEF NTLRTYFPSG KPFIFGPVDS DHYFLFVYDD VIRPCETEND TQLSMTMYGL DRTQTKHWFS DRFISTGTET AAIRKATKLN KVADDSWKLH DLQFEPCGYS INTIRGAEYQ TIHITPEDHC SFASYETNTP AVNYSERINT VLGVFAPIRF SVIVFIDPDS DVGRLYQKGQ NVGVEAEYYP KYELQNRTVN EFAPGYVVMK MNYAMRAEV
Validated NT Sequence
gctacttccg ccctcattgc gtaattcatc ttcatcacca cgtagcctgg ggcaaattca ttgaccgtac ggttctgcaa ttcatactta gggtaatact ccgcttcaac cccaacgttc tgtcccttct ggtacagcct tccaacatca ctgtcaggat caatgaagac aatcacggaa aaacgtattg gagcaaaaac tcccaacacg gtgtttatcc gctccgaata attgacagcg ggtgtattcg tttcgtacga ggcaaaagag cagtgatctt ccggtgttat gtggatcgtc tggtattctg caccacgaat tgtatttata ctatacccac acggctcaaa ttgcaaatca tgcaacttcc atgagtcatc tgccaccttg ttcagttttg tcgccttgcg gatagcggcc gtctctgtgc ctgttgagat aaaacgatcc gagaaccaat gcttcgtctg cgtacgatct agcccgtaca ttgtcatact caactgtgta tcgttctccg tctcacatgg acgaataacg tcgtcataga cgaatagaaa gtaatgatca ctatcgacag gaccaaatat aaaaggcttt cccgatggaa agtacgttct taaagtgttg aattcctctg ccatggagag atgtggtccc ttctgttccc acggaaagct gtagttctta tgcatgaaag agacccattc cacctcccct cgcacatcac taactgcctc caaaacgaaa ggaacggcat taagtagagt cgtcgtgcca catgtgatca ggatgatacg gtcccgcatg acaaacaatg agctttccgt tagcacataa gagcgaatat actcattact ctctttggat acaatttggg catttaacga gcccaccaca cctttccata ccccatctcc aagggcatgg aggccctccg agtgggtctc gtcagtaata cgcatgatca cctccaaacg tttttccggc ccctcaaaac tggccccctg atttgggtcg tagcccttca ctgaacccca catcgccatc aaagagagag ggtccttat
Expressed Protein Sequence
MAHHHHHHML SNKDPLSLMA MWGSVKGYDP NQGASFEGPE KRLEVIMRIT DETHSEGLHA LGDGVWKGVV GSLNAQIVSK ESNEYIRSYV LTESSLFVMR DRIILITCGT TTLLNAVPFV LEAVSDVRGE VEWVSFMHKN YSFPWEQKGP HLSMAEEFNT LRTYFPSGKP FIFGPVDSDH YFLFVYDDVI RPCETENDTQ LSMTMYGLDR TQTKHWFSDR FISTGTETAA IRKATKLNKV ADDSWKLHDL QFEPCGYSIN TIRGAEYQTI HITPEDHCSF ASYETNTPAV NYSERINTVL GVFAPIRFSV IVFIDPDSDV GRLYQKGQNV GVEAEYYPKY ELQNRTVNEF APGYVVMKMN YAMRAEVAEK DSTDPVEE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTAA GCAATAAGGA CCCTCTCTCT TTGATGGCGA TGTGGGGTTC AGTGAAGGGC TACGACCCAA ATCAGGGGGC CAGTTTTGAG GGGCCGGAAA AACGTTTGGA GGTGATCATG CGTATTACTG ACGAGACCCA CTCGGAGGGC CTCCATGCCC TTGGAGATGG GGTATGGAAA GGTGTGGTGG GCTCGTTAAA TGCCCAAATT GTATCCAAAG AGAGTAATGA GTATATTCGC TCTTATGTGC TAACGGAAAG CTCATTGTTT GTCATGCGGG ACCGTATCAT CCTGATCACA TGTGGCACGA CGACTCTACT TAATGCCGTT CCTTTCGTTT TGGAGGCAGT TAGTGATGTG CGAGGGGAGG TGGAATGGGT CTCTTTCATG CATAAGAACT ACAGCTTTCC GTGGGAACAG AAGGGACCAC ATCTCTCCAT GGCAGAGGAA TTCAACACTT TAAGAACGTA CTTTCCATCG GGAAAGCCTT TTATATTTGG TCCTGTCGAT AGTGATCATT ACTTTCTATT CGTCTATGAC GACGTTATTC GTCCATGTGA GACGGAGAAC GATACACAGT TGAGTATGAC AATGTACGGG CTAGATCGTA CGCAGACGAA GCATTGGTTC TCGGATCGTT TTATCTCAAC AGGCACAGAG ACGGCCGCTA TCCGCAAGGC GACAAAACTG AACAAGGTGG CAGATGACTC ATGGAAGTTG CATGATTTGC AATTTGAGCC GTGTGGGTAT AGTATAAATA CAATTCGTGG TGCAGAATAC CAGACGATCC ACATAACACC GGAAGATCAC TGCTCTTTTG CCTCGTACGA AACGAATACA CCCGCTGTCA ATTATTCGGA GCGGATAAAC ACCGTGTTGG GAGTTTTTGC TCCAATACGT TTTTCCGTGA TTGTCTTCAT TGATCCTGAC AGTGATGTTG GAAGGCTGTA CCAGAAGGGA CAGAACGTTG GGGTTGAAGC GGAGTATTAC CCTAAGTATG AATTGCAGAA CCGTACGGTC AATGAATTTG CCCCAGGCTA CGTGGTGATG AAGATGAATT ACGCAATGAG GGCGGAAGTA GCTGAGAAAG ACTCCACAGA TCCCGTGGAA GAGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT