TrcrA.18239.b

farnesyltransferase, putative

CENTER ID: TrcrA.18239.b
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18239.b.B1.GE35228 full length 1 404
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053508369.20
RefSeq: XP_806417.1
UniProt: Q4CWB4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEYMEELYAM VRMKWRLRRI ETGIACNDED LRFCYDILQA VSRSFAVVIM ELDEEMRDAV CIFYLVLRAL DTVEDDMSIP VDFKLRELPK FHEHLHDTTW CMSGVGVGRE RELLERYTHV TRAYSRLGKA YQDVISGICE RMANGMCDFL TRKVETKADY DLYCHYVAGL VGHGLTLLYV SSGLEDVRLA DDLTNANHMG LFLQKTNIIR DFYEDICEVP PRVFWPREIW EKYTDDLHAF KDELHEAKAV ECLNAMVADA LVHVPHVVEY LASLRDPSVF AFSAIPQVMA MATLSLVFNN KDVFHTKVKT TRGATARIFH YSTELQATLQ MLKTYTLRLA ARMNAQDACY DRIEHLVNDA IRAMESHQKP NGESVARSML MRYPALGGHL LYTLVDNVVG YLGK
NT Sequence
atggagtaca tggaggagtt gtacgccatg gtgcgtatga agtggcggct gcgtcgcatt gaaacgggta ttgcgtgtaa cgacgaggat ctccgctttt gctacgacat actgcaggcc gtctcacgtt cctttgcggt tgtaatcatg gagctcgatg aagagatgcg agatgccgtg tgcatttttt accttgtcct tcgtgccctc gacacagtgg aggacgatat gagtattccc gtcgacttta agctgcggga gctgccaaag tttcatgagc acctgcacga cacgacgtgg tgcatgtccg gcgtcggtgt gggccgtgag cgcgagcttc ttgagcgcta cacacacgtc acgcgcgcct actcgaggct tggcaaggca taccaggacg tcatctctgg tatttgcgag cggatggcga atggaatgtg cgacttcctt acgcgaaagg ttgagaccaa ggcggattac gatctctact gccattacgt cgccggactt gttgggcacg gcctcacact tctgtatgta tcgagcgggc tggaggacgt gcggcttgcc gatgacctga cgaacgccaa tcacatggga ctctttttgc agaagacaaa cattattcgt gacttttacg aggacatttg cgaggtgccg ccccgtgtct tttggccacg cgagatatgg gagaagtaca cggacgatct tcacgccttt aaggacgaat tgcatgaggc caaagccgtt gagtgcctga acgcgatggt ggcggatgcc ctggtgcacg tgccgcacgt cgttgagtac ctcgcctccc tgcgggatcc atccgtgttt gccttcagcg ccattcctca ggtgatggcg atggctaccc tttcgctggt atttaacaac aaggacgttt tccacacgaa ggtgaagacg acacgcggtg ccacggctcg catcttccac tactcaacag agctgcaggc gacgctgcag atgctgaaga catataccct ccgtctggct gcacgtatga atgcccaaga tgcctgctac gacagaattg agcatcttgt gaatgacgca atacgtgcta tggagtcaca ccagaagccc aatggcgaga gtgtggcgcg ctctatgttg atgcgctacc ccgcgcttgg tggtcacctg ctgtacactc tcgttgacaa cgttgttgga tatcttggga agtaa
Details for TrcrA.18239.b.B1.GE35228
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 88
Validated AA Sequence
MAHHHHHHME YMEELYAMVR MKWRLRRIET GIACNDEDLR FCYDILQAVS RSFAVVIMEL DEEMRDAVCI FYLVLRALDT VEDDMSIPVD FKLRELPKFH EHLHDTTWCM SGVGVGRERE LLERYTHVTR AYSRLGKAYQ DVISGICERM ANGMCDFLTR KVETKADYDL YCHYVAGLVG HGLTLLYVSS GLEDVRLADD LTNANHMGLF LQKTNIIRDF YEDICEVPPR VFWPREIWEK YTDDLHAFKD ELHXAKAVEC LNAMVADALV HVPHVVEYLA SLRDPSVFAF SAIPXXWRWL PFRWYXTTXT FSXRXXXXXX XXARXXXYXX SCXXXAXXXX XXXXXXAXXX XXXXXXXXXX XXXX
Validated NT Sequence
ttttntnnnn tttaagaagg aganatacca tggctcacca ccaccaccac catatggagt acatggagga gttgtacgcc atggtgcgta tgaagtggcg gctgcgtcgc attgaaacgg gtattgcgtg taacgacgag gatctccgct tttgctacga catactgcag gccgtctcac gttcctttgc ggttgtaatc atggagctcg atgaagagat gcgagatgcc gtgtgcattt tttaccttgt ccttcgtgcc ctcgacacag tggaggacga tatgagtatt cccgtcgact ttaagctgcg ggagctgcca aagtttcatg agcacctgca cgacacgacg tggtgcatgt ccggcgtcgg tgtgggccgt gagcgcgagc ttcttgagcg ctacacacac gtcacgcgcg cctactcgag gcttggcaag gcataccagg acgtcatctc tggtatttgc gagcggatgg cgaatggaat gtgcgacttc cttacgcgaa aggttgagac caaggcggat tacgatctct actgccacta cgtcgccgga cttgttgggc acggcctcac acttctgtat gtatcgagcg ggctggagga cgtgcggctt gccgatgacc tgacgaacgc caatcacatg ggactctttt tgcagaagac aaacattatt cgtgactttt acgaggacat ttgcgaggtg ccgccccgtg tcttttggcc acgcgagata tgggagaagt acacggacga tcttcacgcc tttaaggacg aattgcatga ngccaaagcc gttgagtgcc tgaacgcgat ggtggcggat gccctggtgc acgtgccgca cgtcgttgag tacctcgcct ccctgcggga tccatccgtg tttgccttca gcgccattcc tcnnngatgg cgatggctac cctttcgctg gtatttnaca acnnggacgt tttccnnacg annnnnnnnc nacncgcnnn gcncggctcg cancnnnnac tactnannna gctgcnnnna nnctgcnnnn gntnannnnn nnnncntncn nnnnnntgcn nntnnngnna nnnngnnncn nnnnnnnnnn nnnanctngn nnnnannnnn nnc
Expected Protein Sequence
MAHHHHHHME YMEELYAMVR MKWRLRRIET GIACNDEDLR FCYDILQAVS RSFAVVIMEL DEEMRDAVCI FYLVLRALDT VEDDMSIPVD FKLRELPKFH EHLHDTTWCM SGVGVGRERE LLERYTHVTR AYSRLGKAYQ DVISGICERM ANGMCDFLTR KVETKADYDL YCHYVAGLVG HGLTLLYVSS GLEDVRLADD LTNANHMGLF LQKTNIIRDF YEDICEVPPR VFWPREIWEK YTDDLHAFKD ELHEAKAVEC LNAMVADALV HVPHVVEYLA SLRDPSVFAF SAIPQVMAMA TLSLVFNNKD VFHTKVKTTR GATARIFHYS TELQATLQML KTYTLRLAAR MNAQDACYDR IEHLVNDAIR AMESHQKPNG ESVARSMLMR YPALGGHLLY TLVDNVVGYL GK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggagt acatggagga gttgtacgcc atggtgcgta tgaagtggcg gctgcgtcgc attgaaacgg gtattgcgtg taacgacgag gatctccgct tttgctacga catactgcag gccgtctcac gttcctttgc ggttgtaatc atggagctcg atgaagagat gcgagatgcc gtgtgcattt tttaccttgt ccttcgtgcc ctcgacacag tggaggacga tatgagtatt cccgtcgact ttaagctgcg ggagctgcca aagtttcatg agcacctgca cgacacgacg tggtgcatgt ccggcgtcgg tgtgggccgt gagcgcgagc ttcttgagcg ctacacacac gtcacgcgcg cctactcgag gcttggcaag gcataccagg acgtcatctc tggtatttgc gagcggatgg cgaatggaat gtgcgacttc cttacgcgaa aggttgagac caaggcggat tacgatctct actgccatta cgtcgccgga cttgttgggc acggcctcac acttctgtat gtatcgagcg ggctggagga cgtgcggctt gccgatgacc tgacgaacgc caatcacatg ggactctttt tgcagaagac aaacattatt cgtgactttt acgaggacat ttgcgaggtg ccgccccgtg tcttttggcc acgcgagata tgggagaagt acacggacga tcttcacgcc tttaaggacg aattgcatga ggccaaagcc gttgagtgcc tgaacgcgat ggtggcggat gccctggtgc acgtgccgca cgtcgttgag tacctcgcct ccctgcggga tccatccgtg tttgccttca gcgccattcc tcaggtgatg gcgatggcta ccctttcgct ggtatttaac aacaaggacg ttttccacac gaaggtgaag acgacacgcg gtgccacggc tcgcatcttc cactactcaa cagagctgca ggcgacgctg cagatgctga agacatatac cctccgtctg gctgcacgta tgaatgccca agatgcctgc tacgacagaa ttgagcatct tgtgaatgac gcaatacgtg ctatggagtc acaccagaag cccaatggcg agagtgtggc gcgctctatg ttgatgcgct accccgcgct tggtggtcac ctgctgtaca ctctcgttga caacgttgtt ggatatcttg ggaagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat