SSGCID: Target Page

TrcrA.18238.b

protein kinase, putative

CENTER ID:	TrcrA.18238.b
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.18238.b.B1.GE35226	full length		1	367

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053510119.70
RefSeq:	XP_806834.1
UniProt:	Q4CXH6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

FSIPQEISAA DVGQITFRRF VSQNRFVTDH AVYAVQRIIE EACCTAFYEE KEPLIGWQSL DKLFEVLLQV FKKTGDESIV VGPSMFVAQN GEGGEASNVA AVTAVDAAVA MADSGARGGG ESGGRPGVSR GSNVGGSSNT NAAGGGGERQ AEGKWLLALQ EASRHLCPNA ILLLLQFVHH ATRGHLKSLN VGGKSIAGTF LVVASNASID ATTRIALLRC LPLLQCASQK AASFIRDTRC SVPLLAHAAK SIPVLEESRE DEVMKVLFNL CEDERMAAAL AASDAFLSIA VDRADLAVER HRWTEVVHAI RFIEILLAYS VDSSRVVESR GLLQLLLRLS DGESFPSSVN SAAQRLLNSV QVQTNVL

NT Sequence

ttttcaattc cgcaggaaat ttctgccgcg gatgttgggc aaatcacatt tcgacgtttc gtctcacaga atcgatttgt cacggatcac gcggtgtatg ctgttcaacg cattatcgaa gaggcctgct gcaccgcctt ttacgaggag aaggaaccac tcattgggtg gcaaagttta gacaagctgt ttgaggttct tctgcaggtg tttaaaaaaa caggtgatga gagcatcgtt gtgggtccat cgatgttcgt tgcgcagaac ggagaagggg gagaggcttc aaacgtggcc gccgtcaccg cagtggacgc ggctgttgcc atggcggata gtggtgcacg tggtggtggc gaaagtggtg gtcgacccgg tgtctctcgc ggaagtaacg tcggcggctc cagcaacacg aatgccgcgg gcggtggcgg tgaacggcaa gcggaaggaa agtggctgct ggccttgcaa gaggcgagtc gtcacttatg ccccaacgca attttgcttc ttctgcaatt tgtccaccac gcaacgagag gtcatctgaa gagtctcaat gtggggggaa agagcatcgc ggggactttt ctcgtcgtcg cctcaaacgc ctccattgac gccaccacac gtatcgcact gctccggtgt ctcccattgc tgcagtgtgc atctcaaaag gccgcctcct tcatacgtga cacgcggtgt agcgtgccac tactggcaca tgctgcgaag agcatccctg tcctggagga gtcccgtgag gacgaggtga tgaaggtcct cttcaacctc tgcgaggatg aacgcatggc ggcggcgctg gccgcatctg acgcatttct cagcatagcc gtagatcgag cagacctcgc ggtggagcga catcgttgga cggaggtggt gcacgcgatc cgcttcattg agatcctgct ggcgtactcg gtggattcct cgcgcgtcgt ggagtcgcgt ggccttctgc agctcctcct ccggttgtcc gatggcgaaa gtttccctag cagcgttaac agcgcggcgc agcggctgct caatagtgtg caggttcaaa ccaatgtact atag

Details for TrcrA.18238.b.B1.GE35226

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	84
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMF SIPQEISAAD VGQITFRRFV SQNRFVTDHA VYAVQRIIEE ACCTAFYEEK EPLIGWQSLD KLFEVLLQVF KKTGDESIVV GPSMFVAQNG EGGEASNVAA VTAVDAAVAM ADSGARGGGE SGGRPGVSRG SNVGGSSNTN AAGGGGERQA EGKWLLALQE ASRHLCPNAI LLLLQFVHHA TRGHLKSLNV GGKSIAGTFL VVASNASIDA TTRIALLRCL PLLQCASQKA ASFIRDTRCS VPLLAHAAKS IPVLEESRED XVMKVLFNLC EDERMAAALA ASDAFLSIXV DRAXLXXXRX RWTXXCTRSA SLXSCWXTXX XXXRXXXXXX XAAXXXXXXX XXXXXXXXXX XXXXX

Validated NT Sequence

attttntnnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatgttt tcaattccgc aggaaatttc tgccgcggat gttgggcaaa tcacatttcg acgtttcgtc tcacagaatc gatttgtcac ggatcacgcg gtgtatgctg ttcaacgcat tatcgaagag gcctgctgca ccgcctttta cgaggagaag gaaccactca ttgggtggca aagtttagac aagctgtttg aggttcttct gcaggtgttt aaaaaaacag gtgatgagag catcgttgtg ggtccatcga tgttcgttgc gcagaacgga gaagggggag aggcttcaaa cgtggccgcc gtcaccgcag tggacgcggc tgttgccatg gcggatagtg gtgcacgtgg tggtggcgaa agtggtggtc gacccggtgt ctctcgcgga agtaacgtcg gcggctccag caacacgaat gccgcgggcg gtggcggtga acggcaagcg gaaggaaagt ggctgctggc cttgcaagag gcgagtcgtc acttatgccc caacgcaatt ttgcttcttc tgcaatttgt ccaccacgca acgagaggtc atctgaagag tctcaatgtg gggggaaaga gcatcgcggg gacttttctc gtcgtcgcct caaacgcctc cattgacgcc accacacgta tcgcactgct ccggtgtctc ccattgctgc agtgtgcatc tcaaaaggcc gcctccttca tacgtgacac gcgntgtagc gtgccactac tggcacatgc tgcgaagagc atccctgtcc tggaggagtc ccgtgaggac gangtgatga aggtcctctt caacctctgc gaggatgaac gcatggcggc ggcgctggcc gcatctgacg catttctcag catanccgta gatcgagcag anctnncngn ngnncgacnt cgttggacgg annngtgcac gcgatccgct tcattganat cctgctggng tacncngngn anncncnngc gtcnngnnnn cncnnnnnnn nctgcagcnn cnncctncnn nnnncnangg nnaanntnnn nancannnnn nnnnnnnnnn nnnnnnnncn nnnnngt

Expected Protein Sequence

MAHHHHHHMF SIPQEISAAD VGQITFRRFV SQNRFVTDHA VYAVQRIIEE ACCTAFYEEK EPLIGWQSLD KLFEVLLQVF KKTGDESIVV GPSMFVAQNG EGGEASNVAA VTAVDAAVAM ADSGARGGGE SGGRPGVSRG SNVGGSSNTN AAGGGGERQA EGKWLLALQE ASRHLCPNAI LLLLQFVHHA TRGHLKSLNV GGKSIAGTFL VVASNASIDA TTRIALLRCL PLLQCASQKA ASFIRDTRCS VPLLAHAAKS IPVLEESRED EVMKVLFNLC EDERMAAALA ASDAFLSIAV DRADLAVERH RWTEVVHAIR FIEILLAYSV DSSRVVESRG LLQLLLRLSD GESFPSSVNS AAQRLLNSVQ VQTNVL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttt caattccgca ggaaatttct gccgcggatg ttgggcaaat cacatttcga cgtttcgtct cacagaatcg atttgtcacg gatcacgcgg tgtatgctgt tcaacgcatt atcgaagagg cctgctgcac cgccttttac gaggagaagg aaccactcat tgggtggcaa agtttagaca agctgtttga ggttcttctg caggtgttta aaaaaacagg tgatgagagc atcgttgtgg gtccatcgat gttcgttgcg cagaacggag aagggggaga ggcttcaaac gtggccgccg tcaccgcagt ggacgcggct gttgccatgg cggatagtgg tgcacgtggt ggtggcgaaa gtggtggtcg acccggtgtc tctcgcggaa gtaacgtcgg cggctccagc aacacgaatg ccgcgggcgg tggcggtgaa cggcaagcgg aaggaaagtg gctgctggcc ttgcaagagg cgagtcgtca cttatgcccc aacgcaattt tgcttcttct gcaatttgtc caccacgcaa cgagaggtca tctgaagagt ctcaatgtgg ggggaaagag catcgcgggg acttttctcg tcgtcgcctc aaacgcctcc attgacgcca ccacacgtat cgcactgctc cggtgtctcc cattgctgca gtgtgcatct caaaaggccg cctccttcat acgtgacacg cggtgtagcg tgccactact ggcacatgct gcgaagagca tccctgtcct ggaggagtcc cgtgaggacg aggtgatgaa ggtcctcttc aacctctgcg aggatgaacg catggcggcg gcgctggccg catctgacgc atttctcagc atagccgtag atcgagcaga cctcgcggtg gagcgacatc gttggacgga ggtggtgcac gcgatccgct tcattgagat cctgctggcg tactcggtgg attcctcgcg cgtcgtggag tcgcgtggcc ttctgcagct cctcctccgg ttgtccgatg gcgaaagttt ccctagcagc gttaacagcg cggcgcagcg gctgctcaat agtgtgcagg ttcaaaccaa tgtactatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at