TrcrA.18237.a

long chain fatty acid CoA ligase, putative

CENTER ID: TrcrA.18237.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18237.a.B1.GE35223 full length 1 316
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053510151.19
RefSeq: XP_802927.1
UniProt: Q4CMH8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGGLISTLMD IRNSMSLVPH PVLTGKREQL TLTLPGTETE TTSAIYRHSR TGNEAEHRRI QEEYRARNCV QTLEEKCKTL GSRPAVAYRP LERLERRSVR NADGTERTLD YYHYRGRQVV SYEDLWHYIT SFGSGLLELG VPQRSMITIY EDTRWEWLVS IYGIWTHNMV ATTVYANLGE DALRYALSET DSAVIICSGR TLKMLLTPLR ERGLHPIIVC LDSVPVDVDK GDYQIVAWTD VVKKGAEAKH AINIPTDNDI EAMIMYTSGT SGDPKGVIHT HGSIAAGINA LEERLSDLYN STAETETYCA FLPLAH
NT Sequence
atgggaggct tgatatcaac gttgatggac atacgaaaca gcatgtcttt ggtgccacat ccggtcctta ccggaaagag ggagcagctg acattgacgc tgcctggaac agagacggaa acgacatcgg ccatctaccg ccacagtcga accggaaacg aggcggagca ccggcgaatt caggaggaat accgtgcaag aaattgtgtg caaacgcttg aggagaagtg caaaacattg ggctcacgtc ctgctgttgc ttaccgtccg ctagagcgat tagagcgccg ctccgtccgt aatgcggatg ggactgagcg cacgcttgac tactatcact accgtgggcg tcaggtggtg tcttacgagg atttatggca ttacattacg tccttcggct cggggttact tgagcttggg gtgcctcagc gtagcatgat caccatttat gaagacacgc gatgggaatg gcttgtgtct atttacggta tctggacgca taacatggtc gccacgacgg tctatgcgaa cctcggtgaa gatgccttga gatacgccct ctcggagacc gattcagcgg ttatcatttg cagtggccgg acactgaaga tgcttcttac gccgctccgt gaacgagggc tccatcccat cattgtttgc ctcgatagcg ttcccgttga tgtcgacaag ggtgactatc aaatcgttgc gtggacggat gttgtgaaaa agggcgcgga ggcaaaacat gccataaaca ttcccaccga caacgacatt gaggcgatga ttatgtacac aagtggcacg tccggtgatc caaagggcgt cattcacacc cacggttcga ttgccgcggg tattaatgct cttgaagagc gactaagcga tctttacaat tcgacagcag aaacggaaac gtactgtgcc ttcttgccac ttgcacat
Details for TrcrA.18237.a.B1.GE35223
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 108
Validated AA Sequence
MAHHHHHHMG GLISTLMDLR NSMSLVPHPV LTGKREQLTL TLPGTETETT SAIYRHSRTG NEAEHRRIQE EYRARNCVQT LEEKCKTLGP RPAVAYRPLE RLERRSVRNA DGTERTLDYY HFRGRQVVSY EDLWHYITSF GSGLLELGVP QRSKITIYED TRWEWLVSIY GIWTHNMVAT TVYANLGEDA LRYALSETDS AVIICSGRTL KMLLTLLRER GLHPIIVCLD SVPVDVDKGD YQIVAWTDVV KKGAXAKHAI NIPTDNDIEA MIMYTSGTSG DPKGVIHTHG SIAXGINALX XRLSDLYXXT AXTETYXXXX PLXXXXXXXX XXXXPXXXXX XXXXXXXXXX X
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcacc accaccacca ccatatggga ggcttgatat caacgttgat ggacctgcga aacagcatgt ctttggtgcc acatccggtc cttaccggaa agagggagca gctgacattg acgctgcctg gaacagagac ggaaacgaca tcggccatct accgccacag tcgaactgga aacgaggcgg agcaccggcg aattcaggag gaataccgtg caagaaattg tgtgcaaacg cttgaggaga aatgcaaaac attgggccca cgtcctgctg ttgcttaccg tccgctagag cgattagagc gccgctccgt ccgcaatgcg gatgggactg agcgcacgct tgactactat cacttccgtg ggcgtcaggt ggtgtcttac gaggatttat ggcattacat tacgtccttc ggctcggggt tacttgagct tggggtgcct cagcgtagca agatcaccat ttatgaagac acgcgatggg aatggcttgt gtctatttac ggtatctgga cgcataacat ggtcgccacg acggtctatg cgaacctcgg tgaagatgcc ttgagatacg ccctctcgga gaccgattca gcggttatca tttgcagtgg ccggacactg aagatgcttc tcacgctgct ccgtgaacga gggctacatc ccatcattgt ttgcctcgat agcgttccag ttgatgtcga caagggtgac tatcaaatcg ttgcgtggac ggatgttgtg aaaaagggcg cggangcaaa acatgccata aacattccca ccgacaacga cattgaggcg atgattatgt acacaagtgg cacgtccggc gatccaaagg gcgtcattca cactcacggt tcgattgccn cgggtattaa tgctcttgna nagcgactaa gcgatcttta cnanncgaca gcanaaacng aaacgtactg ngnctnnntg ccncttgnnc nttnantgng nnngannnnn cnnnnnnnnn ccccnnnnnn nnnnncngnn aannnnnnnn nnnnnntnnn nnnnnnnnnn nnnc
Expected Protein Sequence
MAHHHHHHMG GLISTLMDIR NSMSLVPHPV LTGKREQLTL TLPGTETETT SAIYRHSRTG NEAEHRRIQE EYRARNCVQT LEEKCKTLGS RPAVAYRPLE RLERRSVRNA DGTERTLDYY HYRGRQVVSY EDLWHYITSF GSGLLELGVP QRSMITIYED TRWEWLVSIY GIWTHNMVAT TVYANLGEDA LRYALSETDS AVIICSGRTL KMLLTPLRER GLHPIIVCLD SVPVDVDKGD YQIVAWTDVV KKGAEAKHAI NIPTDNDIEA MIMYTSGTSG DPKGVIHTHG SIAAGINALE ERLSDLYNST AETETYCAFL PLAH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggag gcttgatatc aacgttgatg gacatacgaa acagcatgtc tttggtgcca catccggtcc ttaccggaaa gagggagcag ctgacattga cgctgcctgg aacagagacg gaaacgacat cggccatcta ccgccacagt cgaaccggaa acgaggcgga gcaccggcga attcaggagg aataccgtgc aagaaattgt gtgcaaacgc ttgaggagaa gtgcaaaaca ttgggctcac gtcctgctgt tgcttaccgt ccgctagagc gattagagcg ccgctccgtc cgtaatgcgg atgggactga gcgcacgctt gactactatc actaccgtgg gcgtcaggtg gtgtcttacg aggatttatg gcattacatt acgtccttcg gctcggggtt acttgagctt ggggtgcctc agcgtagcat gatcaccatt tatgaagaca cgcgatggga atggcttgtg tctatttacg gtatctggac gcataacatg gtcgccacga cggtctatgc gaacctcggt gaagatgcct tgagatacgc cctctcggag accgattcag cggttatcat ttgcagtggc cggacactga agatgcttct tacgccgctc cgtgaacgag ggctccatcc catcattgtt tgcctcgata gcgttcccgt tgatgtcgac aagggtgact atcaaatcgt tgcgtggacg gatgttgtga aaaagggcgc ggaggcaaaa catgccataa acattcccac cgacaacgac attgaggcga tgattatgta cacaagtggc acgtccggtg atccaaaggg cgtcattcac acccacggtt cgattgccgc gggtattaat gctcttgaag agcgactaag cgatctttac aattcgacag cagaaacgga aacgtactgt gccttcttgc cacttgcaca ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat