SSGCID: Target Page

TrcrA.18235.b

lipase precursor-like protein

CENTER ID:	TrcrA.18235.b
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.18235.b.B1.GE35220	full length		1	214

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053510679.70
RefSeq:	XP_807129.1
UniProt:	Q4CYC4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MWGYLQELCG GKGIEGILIT GHSLGGAMAT LAAANFMSQN SLFTSALKVL LYTFGQPRVG NEAFVNWLLA SFCRGGHESY RVTHKRDPVP HVPPMFVGYL HVPNEVWYDN DGDTVHKNCN DVFGTPCSAL TADEDPNCSG SIVPTSIEDH LKYLGVCTRC SCDPGEAMSD EELRLPPELE WIVAMDYVYQ QSRNMRRFPF SSRFTRSLEA RRRK

NT Sequence

atgtgggggt acctacagga actgtgcggg gggaaaggaa tcgagggaat tctcatcact ggacacagcc ttggcggcgc catggctaca ctcgctgctg ccaattttat gtcgcagaat tcgttgttta ccagtgcact gaaggttttg ctgtacacgt ttgggcagcc gcgcgtggga aacgaggcat tcgtcaactg gcttcttgca tcgttttgcc gcggcggcca tgagtcgtac cgcgtgacgc ataagcggga tcctgtgccc cacgttcccc ccatgttcgt tgggtattta cacgttccca atgaggtatg gtacgacaat gacggggaca cggtgcacaa gaactgcaat gatgtctttg gcacaccgtg ttctgctttg accgccgatg aggaccccaa ttgcagtggc agcatcgttc ccacttccat tgaggaccac ctgaagtatc tgggtgtgtg tacacgctgt tcatgcgatc ccggggaagc aatgagcgac gaggagcttc gcctcccacc cgaacttgaa tggattgttg caatggacta cgtttatcag caatcgagaa atatgaggcg ttttccgttt tcctcacgct ttacaaggag ccttgaagcc cgcagaagaa aatga

Details for TrcrA.18235.b.B1.GE35220

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	24kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	93
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMW GYLQELVAGK GIEGILITGH SLGGAMATLA ASNLMSQNSL FPSALKVLLY TFGQPRVGNE AFVNWLLASF CRGGHESYRV THKRDPVPHV PPMFVGYLHL PNEVWYDNDG DTVHKNCNDV FGTPCSALTT KEDPNCSGSV LPIKVEDHLK YLGVCTRCSC DPGEAMSDEE LRLPPELERI VAMDYVYQQS RNMSRFPFSS RFTRSLEARR RK

Validated NT Sequence

ttntttactt taagaaggag anataccatg gctcaccacc accaccacca tatgtggggg tacctacagg aacttgttgc ggggaaagga atcgagggaa ttctcatcac tggacacagc cttggcggcg ccatggctac actcgctgct tccaatttga tgtcgcagaa ttcgttgttt cccagtgcac tgaaggtttt gctgtacacg tttgggcagc cgcgcgtggg aaacgaggca ttcgtcaact ggcttcttgc atcgttttgc cgcggcggcc atgagtctta ccgcgtgacg cataagcggg atcctgtgcc ccacgttccc cccatgttcg ttgggtattt acacctcccc aatgaggtat ggtacgacaa tgacggggac acggtgcaca agaactgcaa tgatgtcttt ggcacaccgt gttctgcttt gaccaccaag gaggacccca attgcagtgg cagtgttctt cccattaaag ttgaggacca cctgaagtat ctgggtgtgt gcacacgctg ttcatgcgat cccggggaag caatgagcga cgaggagctt cgcctcccac ccgaacttga acggattgtt gcaatggact acgtttatca gcaatcgaga aatatgagtc gttttccgtt ttcctcacgc tttacaagga gccttgaagc ccgcagaaga aaataagtga gtaagatagg atccggnnnn nnnnnccccn nannnnnnnn nnnnnaaaan nnnnnnnnnn nnntgnngnn angngncnga nnnnccnccn tnnnnttnna nccngnnnnn ttnnnganng ntnnnnnnnn nnnngnnnnn nnnngnnnnn nnnnnnnnnn ncnnnnncnn tatannnnng annnangggg nnnnanaann ngnnncangn gncgnnncnn tnnnnannnn nnnnnnnnnn nnnnnnctnn annngnnnnn nnntnnnnnn gnnnnnnnnn gnnnnnngna cnnncnnnnn nnnnnnnnnn tcnnnannnt tnnnnnnnnn ngnnnnnnnt gtnnannnnn nnnnnnnnnn natnnnntnn nnnnnncnnn nnnnnnccnn nnnnnnnnan natttnncnc nnnnnnnnnn ntncnnnnnn naaaa

Expected Protein Sequence

MAHHHHHHMW GYLQELCGGK GIEGILITGH SLGGAMATLA AANFMSQNSL FTSALKVLLY TFGQPRVGNE AFVNWLLASF CRGGHESYRV THKRDPVPHV PPMFVGYLHV PNEVWYDNDG DTVHKNCNDV FGTPCSALTA DEDPNCSGSI VPTSIEDHLK YLGVCTRCSC DPGEAMSDEE LRLPPELEWI VAMDYVYQQS RNMRRFPFSS RFTRSLEARR RK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtggg ggtacctaca ggaactgtgc ggggggaaag gaatcgaggg aattctcatc actggacaca gccttggcgg cgccatggct acactcgctg ctgccaattt tatgtcgcag aattcgttgt ttaccagtgc actgaaggtt ttgctgtaca cgtttgggca gccgcgcgtg ggaaacgagg cattcgtcaa ctggcttctt gcatcgtttt gccgcggcgg ccatgagtcg taccgcgtga cgcataagcg ggatcctgtg ccccacgttc cccccatgtt cgttgggtat ttacacgttc ccaatgaggt atggtacgac aatgacgggg acacggtgca caagaactgc aatgatgtct ttggcacacc gtgttctgct ttgaccgccg atgaggaccc caattgcagt ggcagcatcg ttcccacttc cattgaggac cacctgaagt atctgggtgt gtgtacacgc tgttcatgcg atcccgggga agcaatgagc gacgaggagc ttcgcctccc acccgaactt gaatggattg ttgcaatgga ctacgtttat cagcaatcga gaaatatgag gcgttttccg ttttcctcac gctttacaag gagccttgaa gcccgcagaa gaaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat