TrcrA.18225.a

diphosphomevalonate decarboxylase, putative

CENTER ID: TrcrA.18225.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18225.a.B1.GE35217 full length 1 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053507993.330
RefSeq: XP_820623.1
UniProt: Q4E1U8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANLQVTVEA PINIAFIKYW GKRAGGEKLI LPANDSFSIT LSTHPFRTKT SVVLRDDLEE DTLIINGEKS DVRSTPRIQS VLEYVRSTCP DELKNKRVYI VSENNFPTAA GMASSASGYC ALAAALVRVF NSTANVSMLA RMGSGSACRS TLGGFVIWHK GEKEDGSDCV ATQFVDENYW PEMQVLCAVL QGEKKNTSST AGMQQSLQTS PLMPKRIATT VSERMRTVSE AIKARDFYTF AQIAMSESDD LQAICATTQP QIQYATEDSY AMIRLVKTYN AKKGHPTLAY TFDAGANCFL FVLEKDLPEA VAMLMQHFPT PSERFYFHDA MLLQKIQEAT VPHEYENIID YPKKPFVMLL QSPVGSGVRY LSEGESLISL
NT Sequence
atggcgaacc tgcaagtgac cgtggaggcg ccgatcaaca ttgccttcat caaatattgg ggaaaacggg cgggtgggga gaaactgatt ctccccgcaa atgattcctt cagcattacc ctctccactc atccattccg caccaaaacg tcagtggtac ttcgcgatga tttggaagaa gacacgctta taattaacgg agagaaatcg gatgtgcgaa gtacgcctcg aattcaatcg gtgttggaat atgtgcggag tacgtgccct gatgagctaa aaaacaaacg ggtttatatt gtctccgaga acaactttcc caccgcagct ggaatggcat cttccgcgag tgggtactgc gctcttgcgg cggcgttagt gcgggtcttt aactctactg caaatgtgtc gatgcttgct cgaatgggat ccgggagtgc ctgtcgcagc acccttggtg gctttgtgat ttggcataag ggggaaaaag aagatgggag tgattgcgtg gcaactcaat ttgttgacga aaattactgg cctgagatgc aggtcttgtg cgccgttctc cagggggaga agaaaaacac ttcaagcacg gcaggaatgc agcaatcttt gcagacttct ccactgatgc ccaaacgcat tgccaccact gtctctgagc gtatgcgaac tgtctccgag gcaattaagg cgagggattt ttacacattt gcacaaatcg caatgagtga atcggatgac ctgcaagcaa tatgtgctac gacgcagcct cagattcagt atgctacgga ggacagttat gcgatgatac gtcttgtgaa aacttataat gccaaaaagg gccatcccac attggcatat acgtttgacg cgggggcgaa ttgttttttg tttgttcttg aaaaagatct tccagaggcg gtggcaatgt taatgcaaca cttcccaacc ccatccgaac gattttactt ccatgacgcg atgttgctgc aaaagatcca ggaggccacc gtgcctcacg agtatgaaaa catcatagat tatccgaaaa aaccgtttgt tatgctcctt cagtcgcccg ttgggtcggg tgtgcgttat ctctcggagg gggagtccct catttctttg tag
Details for TrcrA.18225.a.B1.GE35217
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 67
Validated AA Sequence
MASSASGYCA LAAALVRVFN STANVSMLAR MGSGSACRST LGGFVIWHKG EKEDGSDCVA TQFVDENYWP EMQVLCAVLQ GEKKNTSSTA GMQQSLQTSP LMPKRIATTV SERMRTVSEA IKARDFYTFA QIAMSESDDL QAICATTQPQ IQYATEDSYA MIRLVKTYNA KKGHPTLAYT FDAGANCFLF VLEKDLPEAV AMLMQHFPTP SERFYFHDAM LLQKIQEATV PHEYENIIDY PKKPFVMLLQ SPVGSGVRYL
Validated NT Sequence
cgagagataa cgcacacccg acccaacggg cgactgaagg agcataacaa acggtttttt cggataatct atgatgtttt catactcgtg aggcacggtg gcctcctgga tcttttgcag caacatcgcg tcatggaagt aaaatcgttc ggatggggtt gggaagtgtt gcattaacat tgccaccgcc tctggaagat ctttttcaag aacaaacaaa aaacaattcg cccccgcgtc aaacgtatat gccaatgtgg gatggccctt tttggcatta taagttttca caagacgtat catcgcataa ctgtcctccg tagcatactg aatctgaggc tgcgtcgtag cacatattgc ttgcaggtca tccgattcac tcattgcgat ttgtgcaaat gtgtaaaaat ccctcgcctt aattgcctcg gagacagttc gcatacgctc agagacagtg gtggcaatgc gtttgggcat cagtggagaa gtctgcaaag attgctgcat tcctgccgtg cttgaagtgt ttttcttctc cccctggaga acggcgcaca agacctgcat ctcaggccag taattttcgt caacaaattg agttgccacg caatcactcc catcttcttt ttccccctta tgccaaatca caaagccacc aagggtgctg cgacaggcac tcccggatcc cattcgagca agcatcgaca catttgcagt agagttaaag acccgcacta acgccgccgc aagagcgcag tacccactcg cggaagatgc cattccagct gcggtgggaa agttgttctc ggagacaata taaacccgtt tgttttttag ctcatcaggg cacgtactcc gcacatattc caacaccgat tgaattcgag gcgtacttcg cacatccgat ttctctccgt taattataag cgtgtcttct tccaaatcat cgcgaagtac cactgacgtt ttggtgcgga atggatgagt ggagagggta atgctgaagg aatcatttgc ggggagaatc agtttctccc cacccgcccg ttttccccaa tatttgatga aggcaatgtt gatcggcgcc tccacggtca cttgcag
Expected Protein Sequence
MAHHHHHHMA NLQVTVEAPI NIAFIKYWGK RAGGEKLILP ANDSFSITLS THPFRTKTSV VLRDDLEEDT LIINGEKSDV RSTPRIQSVL EYVRSTCPDE LKNKRVYIVS ENNFPTAAGM ASSASGYCAL AAALVRVFNS TANVSMLARM GSGSACRSTL GGFVIWHKGE KEDGSDCVAT QFVDENYWPE MQVLCAVLQG EKKNTSSTAG MQQSLQTSPL MPKRIATTVS ERMRTVSEAI KARDFYTFAQ IAMSESDDLQ AICATTQPQI QYATEDSYAM IRLVKTYNAK KGHPTLAYTF DAGANCFLFV LEKDLPEAVA MLMQHFPTPS ERFYFHDAML LQKIQEATVP HEYENIIDYP KKPFVMLLQS PVGSGVRYLS EGESLISL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga acctgcaagt gaccgtggag gcgccgatca acattgcctt catcaaatat tggggaaaac gggcgggtgg ggagaaactg attctccccg caaatgattc cttcagcatt accctctcca ctcatccatt ccgcaccaaa acgtcagtgg tacttcgcga tgatttggaa gaagacacgc ttataattaa cggagagaaa tcggatgtgc gaagtacgcc tcgaattcaa tcggtgttgg aatatgtgcg gagtacgtgc cctgatgagc taaaaaacaa acgggtttat attgtctccg agaacaactt tcccaccgca gctggaatgg catcttccgc gagtgggtac tgcgctcttg cggcggcgtt agtgcgggtc tttaactcta ctgcaaatgt gtcgatgctt gctcgaatgg gatccgggag tgcctgtcgc agcacccttg gtggctttgt gatttggcat aagggggaaa aagaagatgg gagtgattgc gtggcaactc aatttgttga cgaaaattac tggcctgaga tgcaggtctt gtgcgccgtt ctccaggggg agaagaaaaa cacttcaagc acggcaggaa tgcagcaatc tttgcagact tctccactga tgcccaaacg cattgccacc actgtctctg agcgtatgcg aactgtctcc gaggcaatta aggcgaggga tttttacaca tttgcacaaa tcgcaatgag tgaatcggat gacctgcaag caatatgtgc tacgacgcag cctcagattc agtatgctac ggaggacagt tatgcgatga tacgtcttgt gaaaacttat aatgccaaaa agggccatcc cacattggca tatacgtttg acgcgggggc gaattgtttt ttgtttgttc ttgaaaaaga tcttccagag gcggtggcaa tgttaatgca acacttccca accccatccg aacgatttta cttccatgac gcgatgttgc tgcaaaagat ccaggaggcc accgtgcctc acgagtatga aaacatcata gattatccga aaaaaccgtt tgttatgctc cttcagtcgc ccgttgggtc gggtgtgcgt tatctctcgg agggggagtc cctcatttct ttgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat