SSGCID: Target Page

TrcrA.18224.a

hypothetical protein, conserved

CENTER ID:	TrcrA.18224.a
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.18224.a.B1.GE35215	full length		1	290

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053507645.40
RefSeq:	XP_806082.1
UniProt:	Q4CVD0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEDNNGPKRA RSEAMEDTSS RSTTPLQVDQ STTPEATPAR DPLLEEQMDQ LGVDEDFKAS LRCMSPNTRR DVMNDIIRGQ QQERTTMATP FGLFSILGET SILPLFSGVD VRLSVEEDSE DEQRGSDGSR AHSSSRRMSP SSGADDRSEN SRMETPALRE QVLRSQMLYL LHIMRGSHIA NTISEAIAQR SLGITQDIDD MSYEQLLELQ ERIGFVSKGI TREQMQQCMQ DVPWPKEGSC VVCQSEWQDG TEGNERSVEL KVCHHIFHQR CIEQWLGSNK TCPVCKKDVV

NT Sequence

atggaggaca acaacggtcc caagcgggcg cgcagtgagg cgatggagga tacttccagc aggtcgacga cacccctgca agtggatcag tctacaacgc ccgaggcgac cccggcacgt gacccgttgc tggaggagca gatggaccaa ctcggtgtgg atgaggactt taaggcatct ttgcgttgca tgtcgccgaa tacgcgaagg gatgtgatga acgatattat aaggggccag cagcaggagc ggaccacgat ggcgaccccg tttggattgt tttctatcct gggtgaaacc tccattttac cgcttttcag tggcgtagat gtgcgtctct ccgtagagga ggattcggag gatgagcaga ggggatcgga tggctcgagg gcgcactcct cgtcgcggcg aatgtcccca agcagtgggg ccgatgatag gtctgaaaac tcgagaatgg agacaccggc gttgcgagaa caggttttgc gctctcagat gctttactta cttcacatca tgcgcggctc tcacatcgct aacaccattt ctgaggccat tgcgcagcgg tcgctgggga tcacgcagga tattgatgac atgtcgtatg agcagctttt ggaacttcag gagcggattg ggtttgtcag caagggtatt accagggagc agatgcagca gtgcatgcaa gatgtgccgt ggccgaagga aggcagctgt gtggtgtgcc agtccgaatg gcaagatggc acagaaggca acgagcggag tgtagagctg aaggtgtgcc atcacatttt ccaccaacgc tgcattgagc agtggctggg gtccaacaag acatgcccgg tgtgcaagaa ggacgttgtg tga

Details for TrcrA.18224.a.B1.GE35215

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	33kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	14
PERCENT COVERAGE:	62

Validated AA Sequence

MAHHHHHHME DNNXXXLLXG XXXXXXXXGX XXXXXXXFXX XXXPXXXXXX XXXMXXXXXX XXXXXXCXXX XXPXXGXXXF XXXTSXXXXX DGXXXXIXXX XKPPFXRFSX PXMCVSPXXX XXXXXXXXXX VXXXXLXXXX VPXXXGXXXV XXXXNXXXXX ARTXXALSXX XXTSHHAXLX HXXHHF

Validated NT Sequence

attttgttnn nnttaagaag gaganatacc atggctcacc accaccacca ccatatggag gacaacaacn nnncnnccct ccttcnnggt nnngnnannn nnnnnnnttt nnttggnnnn nnnnnnncnn gnnnnnnnnt cttcnncnnc nnnnnncncc cccntnnnnn ntnnnttnnt nnnnnncnna tgnnttnnnn nnnnntgnnn nnnnntnnnn nnnnnntttg cntcnnntgn ntccnaccnn nnnnnggnnt nnnncnnttt ttnnttgnga cctcnnnnnn ntnnnntnnn gatggcnncn ncgnttgnat tgnnnnnngn ntgaaacctc cattttnccg gttttcanng ccntnnatgt gcgtctctcc gtanannang nttcnnanga nnnncanang nnatnngang gtcnnnnncn nnntcctcnn cncgncgaan gtccccganc nnngnggncn annatnngtc ngannnntnn nnaatgnana cnccgncntt gcnagaacnn gntntgcnct ctcnnatgnt ntacntacnt cacatcatgc ncnnctctna catnnnnnac accatttctg angccnttgc gcancnntcn ntggnnnnca cncnnnannt tnatnannnn ncnnatganc nnctnttnnn ancttcanna cngattgngt nngtcnnnnn ngngtannac cnnnannnga tgnnntnntg cangnnnnnn nnnncnngnn nnnnnnngca nnnnnntgan ntgcnnncna annnnnnnnn gnnnnaannn nnnnagnnnn nnanannnnt tcnnnnnacg nnnnnntgnn cnnnnnnnng nnnncnnncn ngnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnannnn nnnnnngnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccnnnnn gnnnnnnnnn nnnncntcnn nannnnnnnn nnnnnnnnnt nnntg

Expected Protein Sequence

MAHHHHHHME DNNGPKRARS EAMEDTSSRS TTPLQVDQST TPEATPARDP LLEEQMDQLG VDEDFKASLR CMSPNTRRDV MNDIIRGQQQ ERTTMATPFG LFSILGETSI LPLFSGVDVR LSVEEDSEDE QRGSDGSRAH SSSRRMSPSS GADDRSENSR METPALREQV LRSQMLYLLH IMRGSHIANT ISEAIAQRSL GITQDIDDMS YEQLLELQER IGFVSKGITR EQMQQCMQDV PWPKEGSCVV CQSEWQDGTE GNERSVELKV CHHIFHQRCI EQWLGSNKTC PVCKKDVV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggagg acaacaacgg tcccaagcgg gcgcgcagtg aggcgatgga ggatacttcc agcaggtcga cgacacccct gcaagtggat cagtctacaa cgcccgaggc gaccccggca cgtgacccgt tgctggagga gcagatggac caactcggtg tggatgagga ctttaaggca tctttgcgtt gcatgtcgcc gaatacgcga agggatgtga tgaacgatat tataaggggc cagcagcagg agcggaccac gatggcgacc ccgtttggat tgttttctat cctgggtgaa acctccattt taccgctttt cagtggcgta gatgtgcgtc tctccgtaga ggaggattcg gaggatgagc agaggggatc ggatggctcg agggcgcact cctcgtcgcg gcgaatgtcc ccaagcagtg gggccgatga taggtctgaa aactcgagaa tggagacacc ggcgttgcga gaacaggttt tgcgctctca gatgctttac ttacttcaca tcatgcgcgg ctctcacatc gctaacacca tttctgaggc cattgcgcag cggtcgctgg ggatcacgca ggatattgat gacatgtcgt atgagcagct tttggaactt caggagcgga ttgggtttgt cagcaagggt attaccaggg agcagatgca gcagtgcatg caagatgtgc cgtggccgaa ggaaggcagc tgtgtggtgt gccagtccga atggcaagat ggcacagaag gcaacgagcg gagtgtagag ctgaaggtgt gccatcacat tttccaccaa cgctgcattg agcagtggct ggggtccaac aagacatgcc cggtgtgcaa gaaggacgtt gtgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat