TrcrA.18222.a

glutamyl-tRNA synthetase, putative

CENTER ID: TrcrA.18222.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18222.a.B2.GE35213 mature protein 24 600
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18222.a.B2.PS01543 mature protein 24 600

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053506831.70
RefSeq: XP_809232.1
UniProt: Q4D4C4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFRFLKQFGE FIFLGPLLIN GTHSFQKNLY MSAPNANVES DKLILTNAEE GKVVTRFPPE ASGFLHIGHA KAALINSMLA SKFKGKLVLR FDDTNPSKER GHFEQAILDD IATLGISWDI GPTFSSDYMD LMYEKAEELI GKGLAYCDKT PREEMQKCRF DGVPTRYRDI EVEETKRLWN EMKKGSAEGQ ETCLRAKISV DNENKAMRDP VIYRVNETPH ARQGTKYKAY PTYDFACPII DSIEGVTHAL RTNEYHDRND QYYWFCDALN IRKPIVEDFS RLNMEYTVMS KRKLTQFVEN GVVDGWDDPR FPTVRALVRR GLKMNALRKF VQAQGMSKTV NFMEWTKLWF LNTQILDPSV PRYTVVSNSL KVRCAVEGQI HLQECEKLLH KKVPDMGTKK YFKSDVIFLD TEDVALLKEG EEITLMDWGN AYIKNIRSSG ESSLITDADI VLHLEGDVKK TKYKLTWVPE SPNAEVMELV EYDHLLTKKK PDLEEKIDDI IAPVTKFTQE VYAEEAVQTL KKGDTIQIER RGYYIVDSVT PKKVLIYIPD GREKVNHLGA KAQYLKSLSK DGISSAANDL AAKRAAKAAK KALQKQNSQK
NT Sequence
atgtttagat ttttaaaaca gtttggtgag tttatttttt tagggccatt gcttataaac gggactcatt cttttcaaaa aaacttatat atgtcggccc caaatgcgaa tgtggagtct gataaactta tcctaacaaa cgctgaagaa ggaaaggttg tgactcgatt tcctcctgag gccagtggtt tccttcacat tgggcatgca aaggcagcac taataaacag tatgcttgca tccaagttta agggaaagct tgttcttcgc tttgatgata ccaatccttc taaggaaagg ggtcattttg agcaggctat tttagatgat atagcaactc ttgggatttc atgggatatc ggcccgacat tttcttcaga ttacatggac cttatgtatg aaaaagcaga agaacttatt ggaaaaggtc ttgcatattg tgataaaaca ccacgggaag agatgcagaa gtgtagattt gatggggttc ccacacggta tcgtgacata gaggtagagg agacaaaacg cctatggaac gaaatgaaaa aaggaagtgc tgagggccaa gaaacctgtt tgagggcgaa aatttccgtc gataatgaaa ataaggccat gagagatcct gtgatttatc gagtgaatga aacccctcat gctagacaag gaacgaagta taaggcctat ccgacatatg attttgcttg tccaattatt gattccattg aaggggtgac gcacgccttg agaaccaatg agtatcatga tcgcaatgat cagtattatt ggttttgtga tgctttgaac atacgaaaac caatagtgga ggatttttca agactaaata tggagtacac tgttatgtca aagcgaaagc tgactcagtt tgtggagaac ggtgtagtag atggatggga tgatcctcga tttccaacgg ttcgtgcgtt ggttcgacgt ggattaaaaa tgaacgcgtt aagaaagttt gttcaggcac aaggtatgtc aaagacagtt aattttatgg aatggacaaa gctctggttt ttgaatactc aaattctaga tccctctgtc ccacgctata ctgttgtatc gaattcattg aaggtgcgtt gcgccgtgga aggacaaatc catcttcaag agtgtgaaaa acttcttcat aagaaagttc cagatatggg tactaaaaaa tattttaaat ccgatgttat tttccttgat acagaagacg ttgctcttct caaggagggc gaagagatta cgttgatgga ttggggaaat gcgtacatta aaaatattcg ctcatccgga gaatcatctt taattacgga tgccgatatc gttttacatc ttgaaggtga tgtgaaaaaa acgaagtata agttaacttg ggttcccgag agtccgaatg cggaggtgat ggagctggtt gaatatgatc accttcttac aaagaaaaaa ccagatcttg aagaaaaaat tgatgacatt atagccccag taacaaagtt tactcaggag gtatacgcag aggaagccgt tcagactctt aaaaagggtg atacaatcca aattgaacgt cggggatatt acattgtgga ttctgtaaca cccaaaaagg ttcttattta tattccggat gggcgagaaa aagttaatca tttgggtgct aaagctcaat atttgaagtc tttatcgaaa gatggaatat cttccgctgc aaatgatttg gctgcaaagc gagctgcaaa agctgctaaa aaggctttac agaagcaaaa ctcccaaaaa tag
Details for TrcrA.18222.a.B2.GE35213
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 95
Validated AA Sequence
MSAPNANVES DKLILTNAEE GKVVTRFPPE ASGFLHIGHA KAALINSMLA SKFKGKLVLR FDDTNPSKER GHFEQAILDD IATLGISWDI GPTFSSDYMD LMYEKAEELI GKGLAYCDKT PREEMQKCRF DGVPTRYRDI EVEETKRLWN EMKKGSAEGQ ETCLRAKISV DNENKAMRDP VIYRVNETPH ARQGTKYKAY PTYDFACPII DSIEGVTHAL RTNEYHDRND QYYWFCDALN IRKPIVEDFS RLNMEYTVMS KRKLXQFVXN GVVXGWDDPR FXTVRALVRR XXKMNAXXKF VQAQGMSKTV NFMEWTKLWF LNTQILDPSX PRYTVVXNSX KVRCAVEGQI HLQECEKLLH KKVPDMGTKK YFKSDVIFXD TEDVALLKEG EEITLMDWGN AYIKNIRSSG ESSLITDADI VLHLEGDVKK TKYKLTWVPE SPNAEVMELV EYDHLLTKKK PDLEEKIDDI IAPVTKFTQE VYAEEAVQTL KKGDTIQIER RGYYIVDSVT PKKVLIYIPD GREKVNHLGA KAQYLKSLSK DGISSAANDL AAKRAAKAAK KA
Validated NT Sequence
aaagcctttt tagcagcttt tgcagctcgc tttgcagcca aatcatttgc agcggaagat attccatctt tcgataaaga cttcaaatat tgagctttag cacccaaatg attaactttt tctcgcccat ccggaatata aataagaacc tttttgggtg ttacagaatc cacaatgtaa tatccccgac gttcaatttg gattgtatca ccctttttaa gagtctgaac ggcttcctct gcgtatacct cctgagtaaa ctttgttact ggggctataa tgtcatcaat tttttcttca agatctggtt ttttctttgt aagaaggtga tcatattcaa ccagctccat cacctccgca ttcggactct cgggaaccca agttaactta tacttcgttt ttttcacatc accttcaaga tgtaaaacga tatcggcatc cgtaattaaa gatgattctc cggatgagcg aatattttta atgtacgcat ttccccaatc catcaacgta atctcttcgc cctccttgag aagagcaacg tcttctgtat canggaaaat aacatcggat ttaaaatatt ttttagtacc catatctgga actttcttat gaagaagttt ttcacactct tgaagatgga tttgtccttc cacggcgcaa cgcaccttcn atgaattcna tacaacagta tagcgtggga naganggatc tagaatttga gtattcaaaa accagagctt tgtccattcc ataaaattaa ctgtctttga cataccttgt gcctgaacaa actttntnaa cgcgttcatt tttnntnnac gtcgaaccaa cgcacgaacc gttgnaaatc gaggatcatc ccatccntnt actacaccgt tntccacaaa ctgantcagc tttcgctttg acataacagt gtactccata tttagtcttg aaaaatcctc cactattggt tttcgtatgt tcaaagcatc acaaaaccaa taatactgat cattgcgatc atgatactca ttggttctca aggcgtgcgt caccccttca atggaatcaa taattggaca agcaaaatca tatgtcggat aggccttata cttcgttcct tgtctagcat gaggggtttc attcactcga taaatcacag gatctctcat ggccttattt tcattatcga cggaaatttt cgccctcaaa caggtttctt ggccctcagc acttcctttt ttcatttcgt tccataggcg ttttgtctcc tctacctcta tgtcacgata ccgtgtggga accccatcaa atctacactt ctgcatctct tcccgtggtg ttttatcaca atatgcaaga ccttttccaa taagttcttc tgctttttca tacataaggt ccatgtaatc tgaagaaaat gtcgggccga tatcccatga aatcccaaga gttgctatat catctaaaat agcctgctca aaatgacccc tttccttaga aggattggta tcatcaaagc gaagaacaag ctttccttta aacttggatg caagcatact gtttattagt gctgcctttg catgcccaat gtgaaggaaa ccactggcct caggaggaaa tcgagtcaca acctttcctt cttcagcgtt tgttaggata agtttatcag actccacatt cgcatttggg gccgacatat ataagttttt ttga
Expected Protein Sequence
MAHHHHHHMS FQKNLYMSAP NANVESDKLI LTNAEEGKVV TRFPPEASGF LHIGHAKAAL INSMLASKFK GKLVLRFDDT NPSKERGHFE QAILDDIATL GISWDIGPTF SSDYMDLMYE KAEELIGKGL AYCDKTPREE MQKCRFDGVP TRYRDIEVEE TKRLWNEMKK GSAEGQETCL RAKISVDNEN KAMRDPVIYR VNETPHARQG TKYKAYPTYD FACPIIDSIE GVTHALRTNE YHDRNDQYYW FCDALNIRKP IVEDFSRLNM EYTVMSKRKL TQFVENGVVD GWDDPRFPTV RALVRRGLKM NALRKFVQAQ GMSKTVNFME WTKLWFLNTQ ILDPSVPRYT VVSNSLKVRC AVEGQIHLQE CEKLLHKKVP DMGTKKYFKS DVIFLDTEDV ALLKEGEEIT LMDWGNAYIK NIRSSGESSL ITDADIVLHL EGDVKKTKYK LTWVPESPNA EVMELVEYDH LLTKKKPDLE EKIDDIIAPV TKFTQEVYAE EAVQTLKKGD TIQIERRGYY IVDSVTPKKV LIYIPDGREK VNHLGAKAQY LKSLSKDGIS SAANDLAAKR AAKAAKKALQ KQNSQK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt ttcaaaaaaa cttatatatg tcggccccaa atgcgaatgt ggagtctgat aaacttatcc taacaaacgc tgaagaagga aaggttgtga ctcgatttcc tcctgaggcc agtggtttcc ttcacattgg gcatgcaaag gcagcactaa taaacagtat gcttgcatcc aagtttaagg gaaagcttgt tcttcgcttt gatgatacca atccttctaa ggaaaggggt cattttgagc aggctatttt agatgatata gcaactcttg ggatttcatg ggatatcggc ccgacatttt cttcagatta catggacctt atgtatgaaa aagcagaaga acttattgga aaaggtcttg catattgtga taaaacacca cgggaagaga tgcagaagtg tagatttgat ggggttccca cacggtatcg tgacatagag gtagaggaga caaaacgcct atggaacgaa atgaaaaaag gaagtgctga gggccaagaa acctgtttga gggcgaaaat ttccgtcgat aatgaaaata aggccatgag agatcctgtg atttatcgag tgaatgaaac ccctcatgct agacaaggaa cgaagtataa ggcctatccg acatatgatt ttgcttgtcc aattattgat tccattgaag gggtgacgca cgccttgaga accaatgagt atcatgatcg caatgatcag tattattggt tttgtgatgc tttgaacata cgaaaaccaa tagtggagga tttttcaaga ctaaatatgg agtacactgt tatgtcaaag cgaaagctga ctcagtttgt ggagaacggt gtagtagatg gatgggatga tcctcgattt ccaacggttc gtgcgttggt tcgacgtgga ttaaaaatga acgcgttaag aaagtttgtt caggcacaag gtatgtcaaa gacagttaat tttatggaat ggacaaagct ctggtttttg aatactcaaa ttctagatcc ctctgtccca cgctatactg ttgtatcgaa ttcattgaag gtgcgttgcg ccgtggaagg acaaatccat cttcaagagt gtgaaaaact tcttcataag aaagttccag atatgggtac taaaaaatat tttaaatccg atgttatttt ccttgataca gaagacgttg ctcttctcaa ggagggcgaa gagattacgt tgatggattg gggaaatgcg tacattaaaa atattcgctc atccggagaa tcatctttaa ttacggatgc cgatatcgtt ttacatcttg aaggtgatgt gaaaaaaacg aagtataagt taacttgggt tcccgagagt ccgaatgcgg aggtgatgga gctggttgaa tatgatcacc ttcttacaaa gaaaaaacca gatcttgaag aaaaaattga tgacattata gccccagtaa caaagtttac tcaggaggta tacgcagagg aagccgttca gactcttaaa aagggtgata caatccaaat tgaacgtcgg ggatattaca ttgtggattc tgtaacaccc aaaaaggttc ttatttatat tccggatggg cgagaaaaag ttaatcattt gggtgctaaa gctcaatatt tgaagtcttt atcgaaagat ggaatatctt ccgctgcaaa tgatttggct gcaaagcgag ctgcaaaagc tgctaaaaag gctttacaga agcaaaactc ccaaaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for TrcrA.18222.a.B2.PS01543
PURIFICATION DATe: 7/3/2012
CONCENTRATION: 59.04mg/ml
OBSERVED MW: 68kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 110µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MSAPNANVES DKLILTNAEE GKVVTRFPPE ASGFLHIGHA KAALINSMLA SKFKGKLVLR FDDTNPSKER GHFEQAILDD IATLGISWDI GPTFSSDYMD LMYEKAEELI GKGLAYCDKT PREEMQKCRF DGVPTRYRDI EVEETKRLWN EMKKGSAEGQ ETCLRAKISV DNENKAMRDP VIYRVNETPH ARQGTKYKAY PTYDFACPII DSIEGVTHAL RTNEYHDRND QYYWFCDALN IRKPIVEDFS RLNMEYTVMS KRKLXQFVXN GVVXGWDDPR FXTVRALVRR XXKMNAXXKF VQAQGMSKTV NFMEWTKLWF LNTQILDPSX PRYTVVXNSX KVRCAVEGQI HLQECEKLLH KKVPDMGTKK YFKSDVIFXD TEDVALLKEG EEITLMDWGN AYIKNIRSSG ESSLITDADI VLHLEGDVKK TKYKLTWVPE SPNAEVMELV EYDHLLTKKK PDLEEKIDDI IAPVTKFTQE VYAEEAVQTL KKGDTIQIER RGYYIVDSVT PKKVLIYIPD GREKVNHLGA KAQYLKSLSK DGISSAANDL AAKRAAKAAK KA
Validated NT Sequence
aaagcctttt tagcagcttt tgcagctcgc tttgcagcca aatcatttgc agcggaagat attccatctt tcgataaaga cttcaaatat tgagctttag cacccaaatg attaactttt tctcgcccat ccggaatata aataagaacc tttttgggtg ttacagaatc cacaatgtaa tatccccgac gttcaatttg gattgtatca ccctttttaa gagtctgaac ggcttcctct gcgtatacct cctgagtaaa ctttgttact ggggctataa tgtcatcaat tttttcttca agatctggtt ttttctttgt aagaaggtga tcatattcaa ccagctccat cacctccgca ttcggactct cgggaaccca agttaactta tacttcgttt ttttcacatc accttcaaga tgtaaaacga tatcggcatc cgtaattaaa gatgattctc cggatgagcg aatattttta atgtacgcat ttccccaatc catcaacgta atctcttcgc cctccttgag aagagcaacg tcttctgtat canggaaaat aacatcggat ttaaaatatt ttttagtacc catatctgga actttcttat gaagaagttt ttcacactct tgaagatgga tttgtccttc cacggcgcaa cgcaccttcn atgaattcna tacaacagta tagcgtggga naganggatc tagaatttga gtattcaaaa accagagctt tgtccattcc ataaaattaa ctgtctttga cataccttgt gcctgaacaa actttntnaa cgcgttcatt tttnntnnac gtcgaaccaa cgcacgaacc gttgnaaatc gaggatcatc ccatccntnt actacaccgt tntccacaaa ctgantcagc tttcgctttg acataacagt gtactccata tttagtcttg aaaaatcctc cactattggt tttcgtatgt tcaaagcatc acaaaaccaa taatactgat cattgcgatc atgatactca ttggttctca aggcgtgcgt caccccttca atggaatcaa taattggaca agcaaaatca tatgtcggat aggccttata cttcgttcct tgtctagcat gaggggtttc attcactcga taaatcacag gatctctcat ggccttattt tcattatcga cggaaatttt cgccctcaaa caggtttctt ggccctcagc acttcctttt ttcatttcgt tccataggcg ttttgtctcc tctacctcta tgtcacgata ccgtgtggga accccatcaa atctacactt ctgcatctct tcccgtggtg ttttatcaca atatgcaaga ccttttccaa taagttcttc tgctttttca tacataaggt ccatgtaatc tgaagaaaat gtcgggccga tatcccatga aatcccaaga gttgctatat catctaaaat agcctgctca aaatgacccc tttccttaga aggattggta tcatcaaagc gaagaacaag ctttccttta aacttggatg caagcatact gtttattagt gctgcctttg catgcccaat gtgaaggaaa ccactggcct caggaggaaa tcgagtcaca acctttcctt cttcagcgtt tgttaggata agtttatcag actccacatt cgcatttggg gccgacatat ataagttttt ttga
Expressed Protein Sequence
MAHHHHHHMS FQKNLYMSAP NANVESDKLI LTNAEEGKVV TRFPPEASGF LHIGHAKAAL INSMLASKFK GKLVLRFDDT NPSKERGHFE QAILDDIATL GISWDIGPTF SSDYMDLMYE KAEELIGKGL AYCDKTPREE MQKCRFDGVP TRYRDIEVEE TKRLWNEMKK GSAEGQETCL RAKISVDNEN KAMRDPVIYR VNETPHARQG TKYKAYPTYD FACPIIDSIE GVTHALRTNE YHDRNDQYYW FCDALNIRKP IVEDFSRLNM EYTVMSKRKL TQFVENGVVD GWDDPRFPTV RALVRRGLKM NALRKFVQAQ GMSKTVNFME WTKLWFLNTQ ILDPSVPRYT VVSNSLKVRC AVEGQIHLQE CEKLLHKKVP DMGTKKYFKS DVIFLDTEDV ALLKEGEEIT LMDWGNAYIK NIRSSGESSL ITDADIVLHL EGDVKKTKYK LTWVPESPNA EVMELVEYDH LLTKKKPDLE EKIDDIIAPV TKFTQEVYAE EAVQTLKKGD TIQIERRGYY IVDSVTPKKV LIYIPDGREK VNHLGAKAQY LKSLSKDGIS SAANDLAAKR AAKAAKKALQ KQNSQK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTT TTCAAAAAAA CTTATATATG TCGGCCCCAA ATGCGAATGT GGAGTCTGAT AAACTTATCC TAACAAACGC TGAAGAAGGA AAGGTTGTGA CTCGATTTCC TCCTGAGGCC AGTGGTTTCC TTCACATTGG GCATGCAAAG GCAGCACTAA TAAACAGTAT GCTTGCATCC AAGTTTAAGG GAAAGCTTGT TCTTCGCTTT GATGATACCA ATCCTTCTAA GGAAAGGGGT CATTTTGAGC AGGCTATTTT AGATGATATA GCAACTCTTG GGATTTCATG GGATATCGGC CCGACATTTT CTTCAGATTA CATGGACCTT ATGTATGAAA AAGCAGAAGA ACTTATTGGA AAAGGTCTTG CATATTGTGA TAAAACACCA CGGGAAGAGA TGCAGAAGTG TAGATTTGAT GGGGTTCCCA CACGGTATCG TGACATAGAG GTAGAGGAGA CAAAACGCCT ATGGAACGAA ATGAAAAAAG GAAGTGCTGA GGGCCAAGAA ACCTGTTTGA GGGCGAAAAT TTCCGTCGAT AATGAAAATA AGGCCATGAG AGATCCTGTG ATTTATCGAG TGAATGAAAC CCCTCATGCT AGACAAGGAA CGAAGTATAA GGCCTATCCG ACATATGATT TTGCTTGTCC AATTATTGAT TCCATTGAAG GGGTGACGCA CGCCTTGAGA ACCAATGAGT ATCATGATCG CAATGATCAG TATTATTGGT TTTGTGATGC TTTGAACATA CGAAAACCAA TAGTGGAGGA TTTTTCAAGA CTAAATATGG AGTACACTGT TATGTCAAAG CGAAAGCTGA CTCAGTTTGT GGAGAACGGT GTAGTAGATG GATGGGATGA TCCTCGATTT CCAACGGTTC GTGCGTTGGT TCGACGTGGA TTAAAAATGA ACGCGTTAAG AAAGTTTGTT CAGGCACAAG GTATGTCAAA GACAGTTAAT TTTATGGAAT GGACAAAGCT CTGGTTTTTG AATACTCAAA TTCTAGATCC CTCTGTCCCA CGCTATACTG TTGTATCGAA TTCATTGAAG GTGCGTTGCG CCGTGGAAGG ACAAATCCAT CTTCAAGAGT GTGAAAAACT TCTTCATAAG AAAGTTCCAG ATATGGGTAC TAAAAAATAT TTTAAATCCG ATGTTATTTT CCTTGATACA GAAGACGTTG CTCTTCTCAA GGAGGGCGAA GAGATTACGT TGATGGATTG GGGAAATGCG TACATTAAAA ATATTCGCTC ATCCGGAGAA TCATCTTTAA TTACGGATGC CGATATCGTT TTACATCTTG AAGGTGATGT GAAAAAAACG AAGTATAAGT TAACTTGGGT TCCCGAGAGT CCGAATGCGG AGGTGATGGA GCTGGTTGAA TATGATCACC TTCTTACAAA GAAAAAACCA GATCTTGAAG AAAAAATTGA TGACATTATA GCCCCAGTAA CAAAGTTTAC TCAGGAGGTA TACGCAGAGG AAGCCGTTCA GACTCTTAAA AAGGGTGATA CAATCCAAAT TGAACGTCGG GGATATTACA TTGTGGATTC TGTAACACCC AAAAAGGTTC TTATTTATAT TCCGGATGGG CGAGAAAAAG TTAATCATTT GGGTGCTAAA GCTCAATATT TGAAGTCTTT ATCGAAAGAT GGAATATCTT CCGCTGCAAA TGATTTGGCT GCAAAGCGAG CTGCAAAAGC TGCTAAAAAG GCTTTACAGA AGCAAAACTC CCAAAAATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT