SSGCID: Target Page

TrcrA.18221.c

hypothetical protein, conserved

CENTER ID:	TrcrA.18221.c
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.18221.c.B1.GE35212	full length		1	575

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053510683.20
RefSeq:	XP_803282.1
UniProt:	Q4CNI4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYYFRAEKQG TYFEGWSMRL FAFDSQRRHM YYTEREKCVN RSPNGATETH WPTGSPTAPP WKKKFKVTKL VVVSTVHEFH LDDPHLKETD FFTLELHGRT RALASDEPPA EPLLIPAATE GDGLQGATLE PANRDPFRLC QLYELLRDQF STLSRNQGLR ATASAISVSS QSALSETDQV RTIQSPRSTS AKERHIVATI RCCNEEDFRQ VWYIVQMVLG FDRLTIRPYR GLPPYDPRNG IPFGHIPMHL WHTFMELENS VFYVFIRGDL VGHDHEGNAE VWLPNAYLCI THDMVLVMRE NGTIPRWIHL YNVNTFEYNT VAKRPFFAFL TDDPSPDVLF IPKPPVYGPV AISNFSGKKV VLNVCSILRE ACFASNGIRR ATEFLDADWA SSVREYFNTK EKTRRFKFSP LDGFDHSFSC LIPKVHLARV WREVLREQNA RSHDDVAAIP LEGTGGQTIL SGADMKSVRH QMEVERSRSH DEIVGMPLDK LLGHRRERLN ASLEAPKIRP DNFSRNPTQG SEEVVYQEPG AQYICPDTSL KVSNTGREKK FGLEGKVDEL VNASFAAMGI AKPSS

NT Sequence

atgtattact tccgggcgga gaagcagggc acttactttg agggctggtc gatgcgtctc ttcgccttcg actcacagcg gcgccacatg tactacacgg agcgtgagaa gtgcgtcaat cgcagcccca acggtgccac cgagacgcat tggccgacag ggagcccaac cgcaccgccg tggaagaaga agtttaaggt caccaagctg gtggtcgtgt ccactgtgca cgagttccac ctggacgacc cgcacctgaa ggaaacggac ttctttacgc tggagctaca cggcaggacg cgtgccttgg cgagtgatga gccaccggcg gagccgctgc tgatccctgc ggccacggag ggcgatgggt tgcagggagc aactctggaa ccggcgaacc gagacccgtt ccgtctgtgc caactgtacg aattgctgcg agaccagttc tcaacactca gccgcaatca agggctgcgt gcgacagcct cagccatctc agtaagctcc cagtcggctt tgagtgaaac agatcaggtg cgtacaatcc agtccccgcg cagtacctcg gcgaaggagc ggcacatcgt tgccacaata cgctgctgta acgaggagga cttccggcag gtgtggtata tcgtgcaaat ggtgcttggc tttgaccgac tgaccatacg cccctaccgt ggcctcccgc catacgaccc acgcaatggc atcccttttg ggcacattcc catgcatctt tggcacacat ttatggaact ggagaattcc gtcttttatg tttttattcg tggggatctt gtgggccatg atcacgaagg gaacgcagaa gtgtggctgc caaacgcata tctctgcatc actcacgaca tggtattggt gatgcgtgaa aatggaacga ttccgcggtg gattcatctt tataatgtaa atacttttga atataacacc gttgcgaaac gtcccttttt tgcatttctc acggacgatc cgtcaccgga cgtgctcttt attcccaagc caccagtgta tggccccgtc gccatctcga acttctccgg aaaaaaggtg gttcttaacg tgtgctccat tcttcgggag gcgtgctttg catccaacgg catccgccgc gcgacggagt ttttggatgc ggactgggcc agcagcgtgc gcgagtactt caacacaaag gagaagacgc gccgcttcaa gttctcgccc ctcgatggat ttgaccactc cttcagctgc ctcatcccga aggtacatct ggcgagggtg tggcgggagg tcctgcggga gcaaaatgcg cggtcgcatg acgacgttgc ggccatcccg ttggagggca ccggcggtca aacaattctc tccggcgctg acatgaaatc cgtacggcat caaatggagg tggagcgatc gcgctcacac gacgagattg ttggcatgcc gcttgacaag ctgctcggtc atcgccgcga gcggttaaat gcgtctttgg aggcaccgaa gatacggccg gacaacttca gccgcaaccc cacgcagggc tcggaggagg tggtctacca ggagccgggc gcccagtaca tttgccccga cacttcgctg aaggtgtcaa acaccggaag ggagaagaaa tttggcctgg aaggtaaagt ggacgaactc gtgaatgcca gttttgccgc aatgggtatt gccaagcctt caagctga

Details for TrcrA.18221.c.B1.GE35212

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	66kDa
OBSERVED MW:	66kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMY YFRAEKQGTY FEGWSMRLFA FDSQRRHMYY TEREKCVNRS PNGATETHWP TGSPTAPPWK KKFKVTKLVV VSTVHEFHLD DPHLKETDFF TLELHGRTRA LASDEPPAEP LLIPAATEGD GLQGATLEPA NRDPFRLCQL YELLRDQFST LSRNQGLRAT ASAISVSSQS ALSETDQVRT IQSPRSTSAK ERHIVATIRC CNEEDFRQVW YIVQMVLGFD RLTIRPYRGL PPYDPRNGIP FGHIPMHLWH TFMELENSVF YVFIRGDLVG HDHEGNAEVW LPNAYLCITH DMVLVMRENG TIPRWIHLYN VNTFEYNTVA KRPFFAFLTD DPSPDVLFIP KPPVYGPVAI SNFSGKKVVL NVCSILREAC FASNGIRRAT EFLDADWASS VREYFNTKEK TRRFKFSPLD GFDHSFSCLI PKVHLARVWR EVLREQNARS HDDVAAIPLE GTGGQTILSG ADMKSVRHQM EVERSRSHDE IVGMPLDKLL GHRRERLNAS LEAPKIRPDN FSRNPTQGSE EVVYQEPGAQ YICPDTSLKV SNTGREKKFG LEGKVDELVN ASFAAMGIAK PSS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtatt acttccgggc ggagaagcag ggcacttact ttgagggctg gtcgatgcgt ctcttcgcct tcgactcaca gcggcgccac atgtactaca cggagcgtga gaagtgcgtc aatcgcagcc ccaacggtgc caccgagacg cattggccga cagggagccc aaccgcaccg ccgtggaaga agaagtttaa ggtcaccaag ctggtggtcg tgtccactgt gcacgagttc cacctggacg acccgcacct gaaggaaacg gacttcttta cgctggagct acacggcagg acgcgtgcct tggcgagtga tgagccaccg gcggagccgc tgctgatccc tgcggccacg gagggcgatg ggttgcaggg agcaactctg gaaccggcga accgagaccc gttccgtctg tgccaactgt acgaattgct gcgagaccag ttctcaacac tcagccgcaa tcaagggctg cgtgcgacag cctcagccat ctcagtaagc tcccagtcgg ctttgagtga aacagatcag gtgcgtacaa tccagtcccc gcgcagtacc tcggcgaagg agcggcacat cgttgccaca atacgctgct gtaacgagga ggacttccgg caggtgtggt atatcgtgca aatggtgctt ggctttgacc gactgaccat acgcccctac cgtggcctcc cgccatacga cccacgcaat ggcatccctt ttgggcacat tcccatgcat ctttggcaca catttatgga actggagaat tccgtctttt atgtttttat tcgtggggat cttgtgggcc atgatcacga agggaacgca gaagtgtggc tgccaaacgc atatctctgc atcactcacg acatggtatt ggtgatgcgt gaaaatggaa cgattccgcg gtggattcat ctttataatg taaatacttt tgaatataac accgttgcga aacgtccctt ttttgcattt ctcacggacg atccgtcacc ggacgtgctc tttattccca agccaccagt gtatggcccc gtcgccatct cgaacttctc cggaaaaaag gtggttctta acgtgtgctc cattcttcgg gaggcgtgct ttgcatccaa cggcatccgc cgcgcgacgg agtttttgga tgcggactgg gccagcagcg tgcgcgagta cttcaacaca aaggagaaga cgcgccgctt caagttctcg cccctcgatg gatttgacca ctccttcagc tgcctcatcc cgaaggtaca tctggcgagg gtgtggcggg aggtcctgcg ggagcaaaat gcgcggtcgc atgacgacgt tgcggccatc ccgttggagg gcaccggcgg tcaaacaatt ctctccggcg ctgacatgaa atccgtacgg catcaaatgg aggtggagcg atcgcgctca cacgacgaga ttgttggcat gccgcttgac aagctgctcg gtcatcgccg cgagcggtta aatgcgtctt tggaggcacc gaagatacgg ccggacaact tcagccgcaa ccccacgcag ggctcggagg aggtggtcta ccaggagccg ggcgcccagt acatttgccc cgacacttcg ctgaaggtgt caaacaccgg aagggagaag aaatttggcc tggaaggtaa agtggacgaa ctcgtgaatg ccagttttgc cgcaatgggt attgccaagc cttcaagctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat