TrcrA.18221.a

hypothetical protein, conserved

CENTER ID: TrcrA.18221.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18221.a.B1.GE35210 full length 1 575
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053509011.60
RefSeq: XP_812573.1
UniProt: Q4DDX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYYFRAEKQG TYFEGWSMRL FAFDSQRRHM YYTEREKCVN RSPHSATETH WPTGSPTAPP WKKKFKVTKL VVVSTVHEFR LDDPHLKETD FFTLELHGRT RALASDEPPA EPLLIPAATE GDGSQGATLE QANRDPFRLC ELYELLRDQF STLSRNQGLR ATVSAISVSS QSALSETDQV RTIQSPRSTS AKERHIVATI RCCNEEDFRQ VWYIVQMVLG FDRLTIRPYR GLPPYDPRNG IPFGHIPMHL WHTFMELENS VFYVFIRGDL VGHDHEGNAE VWLPNAYLCI THDMVLVMRE NGTIPRWIHL YNVNTFEYNT VAKRPFFAFL TDDPSPDVLF IPKPPVYGPV AISNFSGKKV VLNVCSILRE ACFASNGIRR ATEFLDADWA SSVRAYFNTK EKTRRFKFSP LDGFDHSFSC LIPKVHLARV WREVLREQNA RSHDDVAAIP LEGTGGQTIL SGADMKSVRH QMEVERSRSH DEIVGMPLDK LLGLRRERLE ASLEAPKIRP DNFNRNPTQG SEEVVYQEPG AQYICPDTSL KVSNTGREKK FGLEGKVNEL VNASFAAMGI AKPSS
NT Sequence
atgtattact tccgggcgga gaagcagggc acttactttg agggctggtc gatgcgtctc ttcgccttcg actcacagcg gcgccacatg tactacacgg agcgtgagaa gtgtgtcaat cgcagccccc acagtgccac cgagacgcat tggccgacag ggagcccaac cgcaccgccg tggaagaaga agtttaaggt caccaagctg gtggtcgttt ccactgtgca cgagttccgc ctggacgacc cgcacctgaa ggaaacggac ttctttacgc tggagctaca cggcaggacg cgtgccttgg cgagtgatga gccaccggcg gagccgctgc tgatccctgc ggccacggag ggcgatgggt cgcagggagc aactctggaa caggcgaacc gagacccgtt ccgtctgtgc gaactgtatg aattgctgcg agatcagttc tcgacactca gccgcaatca agggctgcgt gcgacagtct cagcaatctc agtaagctcc cagtcggctt taagtgaaac agatcaggtg cgtacaatcc agtccccgcg cagtacctcg gcgaaggagc ggcacattgt tgccacaata cgctgctgta acgaggagga ctttcggcag gtgtggtata tcgtgcaaat ggtgcttggc tttgaccgac tgaccatacg cccctaccgt ggactcccgc cgtacgaccc acgcaatggc atcccttttg ggcacattcc catgcatctt tggcacacat ttatggaact ggagaattcc gtcttttatg ttttcattcg tggggatctt gtgggccatg atcacgaagg gaacgcagaa gtgtggctgc caaacgcata tctctgcatc actcacgaca tggtattggt gatgcgtgaa aatggaacga ttccgcggtg gattcatctt tataatgtaa atacctttga atataacacc gttgcgaaac gtcccttttt tgcatttctc acggacgatc catcaccgga cgttctcttt attcccaagc caccggtgta tggccccgtc gccatctcga acttctccgg aaaaaaggtg gttcttaacg tgtgctccat tcttcgggag gcgtgctttg catccaacgg catccgccgc gcgacggagt ttttggatgc ggactgggcc agcagcgtgc gcgcgtactt caacacgaag gagaagacgc gtcgcttcaa gttctcgccc ctcgatggat ttgaccactc cttcagctgc ctcatcccga aggtacatct ggcgagggtg tggcgggagg tcctgcggga gcaaaacgcg cggtcgcatg acgacgttgc ggccatcccg ttggagggca ccggcggcca aacaattctc tccggcgctg acatgaaatc cgtacggcat caaatggagg tggagcgatc gcgctcacac gacgagattg tcggcatgcc gcttgacaag ctgctcggtc ttcgccgcga gcggttggag gcgtcgttgg aggcaccgaa gatacggccg gacaacttca accgcaaccc cacgcagggc tcggaggagg tggtctacca ggagccgggc gcccagtaca tttgccccga cacgtcgctg aaggtgtcaa acaccggcag ggagaagaaa ttcggcctgg aaggtaaagt gaacgaactc gtgaatgcca gttttgccgc aatgggtatc gccaagccgt caagctga
Details for TrcrA.18221.a.B1.GE35210
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 52
Validated AA Sequence
MAHHHHHHMY YFRAEKQGTY FEGWSMRLFA FDSQRRHMYY TEREKCVNRS PHSATETHWP TGSPTAPPWK KKFKVTKLVV VSTVHEFRLD DPHLKETDFF TLELHGRTRA LASDEPPAEP LLIPAATEGD GSQGATLEQA NRDPFRLCEL YELLRDQFST LSRNQGLRAT VSAISVSSQS ALSETDQVRT IQSPRSTSAK ERHIVATIRC CNEEDFRQVW YIVQMVLGFD RLTIRPYRGL PPYDPRNGIP FGHIPMHLWH TFMELENSVF YVFIRGDLVG HDHEXERRXV AAKRISLXHS RXGXGXA
Validated NT Sequence
ttttgtnnnn nttaagaagg agatatacca tggctcacca ccaccaccac catatgtatt acttccgggc ggagaagcag ggcacttact ttgagggctg gtcgatgcgt ctcttcgcct tcgactcaca gcggcgccac atgtactaca cggagcgtga gaagtgtgtc aatcgcagcc cccacagtgc caccgagacg cattggccga cagggagccc aaccgcaccg ccgtggaaga agaagtttaa ggtcaccaag ctggtggtcg tttccactgt gcacgagttc cgcctggacg acccgcacct gaaggaaacg gacttcttta cgctggagct acacggcagg acgcgtgcct tggcgagtga tgagccaccg gcggagccgc tgctgatccc tgcggccacg gagggcgatg ggtcgcaggg agcaactctg gaacaggcga accgagaccc gttccgtctg tgcgaactgt atgaattgct gcgagatcag ttctcgacac tcagccgcaa tcaagggctg cgtgcgacag tctcagcaat ctcagtaagc tcccagtcgg ctttaagtga aacagatcag gtgcgtacaa tccagtcccc gcgcagtacc tcggcgaagg agcggcacat tgttgccaca atacgctgct gtaacgagga ggactttcgg caggtgtggt atatcgtgca aatggtgctt ggctttgacc gactgaccat acgcccctac cgtggactcc cgccgtacga cccacgcaat ggcatccctt ttgggcacat tcccatgcat ctttggcaca catttatgga actggagaat tccgtctttt atgtttttat tcgtggggat cttgtgggcc atgatcacga angggaacgc agaantgtgg ctgccaaacg catatctctg cntcactcac gacntggnan tggtgangcn tgaaatggna cgantccgcg gnggantcat cttnanatgt aantacnntt nananannnn tnncgaaacg nncntttttg cattnnnnnn ngannancnt cncnnnnnnn cncntnnnnn cnannannnn ccnnnnnccn tnnnnnnnnt nncnnnaaan nnngnnnnnc nnnnnnntnn nnnnnnnngn gnnnnnnnnn nnnnnnnnnn nncnnnnnnn nnnnnntntt
Expected Protein Sequence
MAHHHHHHMY YFRAEKQGTY FEGWSMRLFA FDSQRRHMYY TEREKCVNRS PHSATETHWP TGSPTAPPWK KKFKVTKLVV VSTVHEFRLD DPHLKETDFF TLELHGRTRA LASDEPPAEP LLIPAATEGD GSQGATLEQA NRDPFRLCEL YELLRDQFST LSRNQGLRAT VSAISVSSQS ALSETDQVRT IQSPRSTSAK ERHIVATIRC CNEEDFRQVW YIVQMVLGFD RLTIRPYRGL PPYDPRNGIP FGHIPMHLWH TFMELENSVF YVFIRGDLVG HDHEGNAEVW LPNAYLCITH DMVLVMRENG TIPRWIHLYN VNTFEYNTVA KRPFFAFLTD DPSPDVLFIP KPPVYGPVAI SNFSGKKVVL NVCSILREAC FASNGIRRAT EFLDADWASS VRAYFNTKEK TRRFKFSPLD GFDHSFSCLI PKVHLARVWR EVLREQNARS HDDVAAIPLE GTGGQTILSG ADMKSVRHQM EVERSRSHDE IVGMPLDKLL GLRRERLEAS LEAPKIRPDN FNRNPTQGSE EVVYQEPGAQ YICPDTSLKV SNTGREKKFG LEGKVNELVN ASFAAMGIAK PSS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtatt acttccgggc ggagaagcag ggcacttact ttgagggctg gtcgatgcgt ctcttcgcct tcgactcaca gcggcgccac atgtactaca cggagcgtga gaagtgtgtc aatcgcagcc cccacagtgc caccgagacg cattggccga cagggagccc aaccgcaccg ccgtggaaga agaagtttaa ggtcaccaag ctggtggtcg tttccactgt gcacgagttc cgcctggacg acccgcacct gaaggaaacg gacttcttta cgctggagct acacggcagg acgcgtgcct tggcgagtga tgagccaccg gcggagccgc tgctgatccc tgcggccacg gagggcgatg ggtcgcaggg agcaactctg gaacaggcga accgagaccc gttccgtctg tgcgaactgt atgaattgct gcgagatcag ttctcgacac tcagccgcaa tcaagggctg cgtgcgacag tctcagcaat ctcagtaagc tcccagtcgg ctttaagtga aacagatcag gtgcgtacaa tccagtcccc gcgcagtacc tcggcgaagg agcggcacat tgttgccaca atacgctgct gtaacgagga ggactttcgg caggtgtggt atatcgtgca aatggtgctt ggctttgacc gactgaccat acgcccctac cgtggactcc cgccgtacga cccacgcaat ggcatccctt ttgggcacat tcccatgcat ctttggcaca catttatgga actggagaat tccgtctttt atgttttcat tcgtggggat cttgtgggcc atgatcacga agggaacgca gaagtgtggc tgccaaacgc atatctctgc atcactcacg acatggtatt ggtgatgcgt gaaaatggaa cgattccgcg gtggattcat ctttataatg taaatacctt tgaatataac accgttgcga aacgtccctt ttttgcattt ctcacggacg atccatcacc ggacgttctc tttattccca agccaccggt gtatggcccc gtcgccatct cgaacttctc cggaaaaaag gtggttctta acgtgtgctc cattcttcgg gaggcgtgct ttgcatccaa cggcatccgc cgcgcgacgg agtttttgga tgcggactgg gccagcagcg tgcgcgcgta cttcaacacg aaggagaaga cgcgtcgctt caagttctcg cccctcgatg gatttgacca ctccttcagc tgcctcatcc cgaaggtaca tctggcgagg gtgtggcggg aggtcctgcg ggagcaaaac gcgcggtcgc atgacgacgt tgcggccatc ccgttggagg gcaccggcgg ccaaacaatt ctctccggcg ctgacatgaa atccgtacgg catcaaatgg aggtggagcg atcgcgctca cacgacgaga ttgtcggcat gccgcttgac aagctgctcg gtcttcgccg cgagcggttg gaggcgtcgt tggaggcacc gaagatacgg ccggacaact tcaaccgcaa ccccacgcag ggctcggagg aggtggtcta ccaggagccg ggcgcccagt acatttgccc cgacacgtcg ctgaaggtgt caaacaccgg cagggagaag aaattcggcc tggaaggtaa agtgaacgaa ctcgtgaatg ccagttttgc cgcaatgggt atcgccaagc cgtcaagctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat