SSGCID: Target Page

TrcrA.18220.b

dipeptidyl-peptidase, putative

CENTER ID:	TrcrA.18220.b
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.18220.b.B1.GE35208	full length		1	677

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053508699.120
RefSeq:	XP_812655.1
UniProt:	Q4DE42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQKVVVSTLP NKVKTIENVF IELRDATRLS CRLWLPADDV PRPAILEYIP YRKRDGTRGR DEPMHGYFAG HGYAAVRVDM RGSGESDGLM HDEYLQQEQD DAMEVIEWIS RQKWCNGNVG MMGKSWGGFN SLQVAVRRPP ALKAIITVGF TDNRFTDGIH WQGGCLLNDN FWWGSIVLAY QARPIDPEIV GDRWKEMWLQ RLENMPVNIA DWAEHQKYDD YWKHGSVCEN YDSIQIPVMA VDGWADGYTN AVFNLLSNLK VPRKGFVGPW AHVYPQDGVP QPAMGFLQEA VKWWDHWLKG TENNVMDGPM IQVWMEHEMK PSVHRPVSEG HWVGVEAWPS KDVTEETMRL ECGRIVDFAH AVRQEPASVL LRTPLNHGLL AGEWMGAGVS GETPADQRMD DGMAMVFDAA ELQHELEILG RPILEVELTC DKPKAMLFAQ LSDVSPDGAV SRISFGVKNV TQAAGDQRIE YLQEGKRVRV RVLLNYCAHR FTKGHRIRLS LANSQWPMFW VSPEINTLSL DLSTATLRLP TYRGPAIAGP NPQPETAAPT PLTVLRAGFV DRSITYDIVK DEWTCITDGV GGVFGDGVYR FDEIGTAVEH NLRRELRLSN KDPLSARYDI KQKMKMGRPG WEADIDIDTR QTCDANYLYV TAEIQAKMND EIVFEKNWSR RVYREGM

NT Sequence

atgcagaagg ttgttgtctc cacgcttcca aacaaagtca aaaccatcga aaatgtcttt attgaactaa gggatgccac gcgactttcg tgccggctgt ggcttccggc agatgatgtg ccccgaccag ccatattgga gtacatccct tatcgcaagc gcgatggtac acgtggtcgt gatgagccca tgcacggcta ctttgcaggc catggctacg cggctgttcg tgtggacatg cgtgggagcg gtgaatcgga tggccttatg catgacgaat acctgcaaca ggagcaagac gatgctatgg aggtgattga atggatcagc aggcagaaat ggtgtaatgg caatgttggc atgatgggga agtcttgggg aggcttcaat tctcttcaag ttgcggtgcg tcgtccacct gctttaaaag ctatcatcac ggtcggattt accgacaatc gctttacgga tggcattcat tggcagggtg gctgtctcct caacgataac ttctggtggg gatccattgt gttggcgtat caggcacgtc caatcgatcc agaaattgtt ggtgatcgtt ggaaggaaat gtggcttcaa cgcctggaga atatgcccgt caacattgct gactgggctg agcaccaaaa atacgatgac tactggaagc atggctctgt ttgcgaaaat tacgattcca ttcagatacc tgtcatggct gtagatggct gggcggacgg ctacacgaat gccgttttta acctgctgtc aaacctgaag gttccccgga agggatttgt ggggccttgg gcacacgttt acccgcagga tggcgtcccg cagccggcaa tggggttttt acaggaggct gtgaagtggt gggatcattg gctaaaaggt acggagaata acgtgatgga tggaccgatg attcaggttt ggatggagca tgaaatgaaa cctagtgttc atcgccctgt tagcgaaggc cattgggttg gtgtggaggc atggccttct aaagatgtta cggaggaaac gatgcgactt gaatgcggtc gtatcgtaga ttttgctcac gccgtgcgac aggagcctgc ctctgttctg ctcagaactc cattgaatca cggtcttctt gccggtgagt ggatgggcgc tggggtttca ggcgaaactc ccgcggatca acgtatggac gacggcatgg cgatggtttt cgacgccgcg gagctgcagc acgagctgga gattttgggt cgtcccatcc tcgaggtgga gttaacgtgt gacaagccaa aggcgatgct gttcgcccaa ctcagcgacg tctcgccgga tggcgcagtg agtcgcattt cctttggcgt gaagaacgtg acacaggccg caggcgatca acgaattgaa tacctccaag aggggaagcg ggttcgtgtc agggttctgc tgaactattg cgctcatcgt tttacgaaag gccatcgtat tcgcctctct cttgcgaaca gccaatggcc aatgttttgg gtatcgcctg agatcaacac cctgtcgttg gacctcagca cggccacgct gcggctaccc acgtaccgcg ggccggccat tgccggtcca aacccgcagc cagaaacagc cgctcccaca cccctcacgg tcctgcgtgc ggggtttgtg gatcggtcca tcacgtacga cattgttaag gatgagtgga catgcataac agatggtgtg ggcggtgtct ttggcgatgg agtttaccgc ttcgatgaaa ttggcacagc cgtggagcac aacttgaggc gagagctaag actctccaac aaggacccgc tctccgcaag gtacgacatc aagcagaaga tgaagatggg gcgccctgga tgggaggcgg atattgacat tgacactcgc caaacgtgtg acgcgaacta tctttacgta accgcggaga tacaagcaaa gatgaatgac gagatcgtct tcgagaagaa ttggtcgcgt cgagtgtacc gtgaagggat gtga

Details for TrcrA.18220.b.B1.GE35208

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	77kDa
OBSERVED MW:	77kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	43

Validated AA Sequence

Validated NT Sequence

anattttgtt tactttaaga aggagatata ccatggctca ccaccaccac caccatatgc agaaggttgt tgtctccacg cttccaaaca aagtcaaaac catcgaaaat gtctttattg aactaaggga tgccacgcga ctttcgtgcc ggctgtggct tccggcagat gatgtgcccc gaccagccat attggagtac atcccttatc gcaagcgcga tggtacacgt ggtcgtgatg agcccatgca cggctacttt gcaggccatg gctacgcggc tgttcgtgtg gacatgcgtg ggagcggtga atcggatggc cttatgcatg acgaatacct gcaacaggag caagacgatg ctatggaggt gattgaatgg atcagcaggc agaaatggtg taatggcaat gttggcatga tggggaagtc ttggggaggc ttcaattctc ttcaagttgc ggtgcgtcgt ccacctgctt taaaagctat catcacggtc ggatttaccg acaatcgctt tacggatggc attcattggc agggtggctg tctcctcaac gataacttct ggtggggatc cattgtgttg gcgtatcagg cacgtccaat cgatccagaa attgttggtg atcgttggaa ggaaatgtgg cttcaacgcc tggagaatat gcccgtcaac attgctgact gggctgagca ccaaaaatac gatgactact ggaagcatgg ctctgtttgc gaaaattacg attccattca gatacctgtc atggctgtag atggctgggc ggacggctac acgaatgccg tttttaacct gctgtcaaac ctgnanggnt ccccggaagg gatttgtggg gccttgggca cacgtttacc cgcnnntggc gtcccgcagc cngcaatggg gtttttacag gnnntgtgaa gtggtgggat cattggctaa annnanngan anangtgatg gnnggacnat gantcnttnn nnngagcatg aatgnaanct anngntcntc gcccnnntnn cnannnntgg nnnnnnnnnn ngnangnnnn nnnaanangn tnnngnnnan nnanncnann nnnnnnnnnn nnnnnannnn nnnnnnnnnn nncnccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cnnnntnnnn nnnnnnnnnn annnnnnnnc nnnnnnt

Expected Protein Sequence

MAHHHHHHMQ KVVVSTLPNK VKTIENVFIE LRDATRLSCR LWLPADDVPR PAILEYIPYR KRDGTRGRDE PMHGYFAGHG YAAVRVDMRG SGESDGLMHD EYLQQEQDDA MEVIEWISRQ KWCNGNVGMM GKSWGGFNSL QVAVRRPPAL KAIITVGFTD NRFTDGIHWQ GGCLLNDNFW WGSIVLAYQA RPIDPEIVGD RWKEMWLQRL ENMPVNIADW AEHQKYDDYW KHGSVCENYD SIQIPVMAVD GWADGYTNAV FNLLSNLKVP RKGFVGPWAH VYPQDGVPQP AMGFLQEAVK WWDHWLKGTE NNVMDGPMIQ VWMEHEMKPS VHRPVSEGHW VGVEAWPSKD VTEETMRLEC GRIVDFAHAV RQEPASVLLR TPLNHGLLAG EWMGAGVSGE TPADQRMDDG MAMVFDAAEL QHELEILGRP ILEVELTCDK PKAMLFAQLS DVSPDGAVSR ISFGVKNVTQ AAGDQRIEYL QEGKRVRVRV LLNYCAHRFT KGHRIRLSLA NSQWPMFWVS PEINTLSLDL STATLRLPTY RGPAIAGPNP QPETAAPTPL TVLRAGFVDR SITYDIVKDE WTCITDGVGG VFGDGVYRFD EIGTAVEHNL RRELRLSNKD PLSARYDIKQ KMKMGRPGWE ADIDIDTRQT CDANYLYVTA EIQAKMNDEI VFEKNWSRRV YREGM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaga aggttgttgt ctccacgctt ccaaacaaag tcaaaaccat cgaaaatgtc tttattgaac taagggatgc cacgcgactt tcgtgccggc tgtggcttcc ggcagatgat gtgccccgac cagccatatt ggagtacatc ccttatcgca agcgcgatgg tacacgtggt cgtgatgagc ccatgcacgg ctactttgca ggccatggct acgcggctgt tcgtgtggac atgcgtggga gcggtgaatc ggatggcctt atgcatgacg aatacctgca acaggagcaa gacgatgcta tggaggtgat tgaatggatc agcaggcaga aatggtgtaa tggcaatgtt ggcatgatgg ggaagtcttg gggaggcttc aattctcttc aagttgcggt gcgtcgtcca cctgctttaa aagctatcat cacggtcgga tttaccgaca atcgctttac ggatggcatt cattggcagg gtggctgtct cctcaacgat aacttctggt ggggatccat tgtgttggcg tatcaggcac gtccaatcga tccagaaatt gttggtgatc gttggaagga aatgtggctt caacgcctgg agaatatgcc cgtcaacatt gctgactggg ctgagcacca aaaatacgat gactactgga agcatggctc tgtttgcgaa aattacgatt ccattcagat acctgtcatg gctgtagatg gctgggcgga cggctacacg aatgccgttt ttaacctgct gtcaaacctg aaggttcccc ggaagggatt tgtggggcct tgggcacacg tttacccgca ggatggcgtc ccgcagccgg caatggggtt tttacaggag gctgtgaagt ggtgggatca ttggctaaaa ggtacggaga ataacgtgat ggatggaccg atgattcagg tttggatgga gcatgaaatg aaacctagtg ttcatcgccc tgttagcgaa ggccattggg ttggtgtgga ggcatggcct tctaaagatg ttacggagga aacgatgcga cttgaatgcg gtcgtatcgt agattttgct cacgccgtgc gacaggagcc tgcctctgtt ctgctcagaa ctccattgaa tcacggtctt cttgccggtg agtggatggg cgctggggtt tcaggcgaaa ctcccgcgga tcaacgtatg gacgacggca tggcgatggt tttcgacgcc gcggagctgc agcacgagct ggagattttg ggtcgtccca tcctcgaggt ggagttaacg tgtgacaagc caaaggcgat gctgttcgcc caactcagcg acgtctcgcc ggatggcgca gtgagtcgca tttcctttgg cgtgaagaac gtgacacagg ccgcaggcga tcaacgaatt gaatacctcc aagaggggaa gcgggttcgt gtcagggttc tgctgaacta ttgcgctcat cgttttacga aaggccatcg tattcgcctc tctcttgcga acagccaatg gccaatgttt tgggtatcgc ctgagatcaa caccctgtcg ttggacctca gcacggccac gctgcggcta cccacgtacc gcgggccggc cattgccggt ccaaacccgc agccagaaac agccgctccc acacccctca cggtcctgcg tgcggggttt gtggatcggt ccatcacgta cgacattgtt aaggatgagt ggacatgcat aacagatggt gtgggcggtg tctttggcga tggagtttac cgcttcgatg aaattggcac agccgtggag cacaacttga ggcgagagct aagactctcc aacaaggacc cgctctccgc aaggtacgac atcaagcaga agatgaagat ggggcgccct ggatgggagg cggatattga cattgacact cgccaaacgt gtgacgcgaa ctatctttac gtaaccgcgg agatacaagc aaagatgaat gacgagatcg tcttcgagaa gaattggtcg cgtcgagtgt accgtgaagg gatgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat