SSGCID: Target Page

TrcrA.18218.b

protein phosphatase 2C-like protein

CENTER ID:	TrcrA.18218.b
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.18218.b.B1.GE35206	full length		1	732

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053508865.4
RefSeq:	XP_810418.1
UniProt:	Q4D7R5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMFMSVRKPP LSSSKGTAKN PALRKGDMGT GHETSQGSIP TVPKTVPLRN NTRCSSGNPF LYGSQRGGAL PYQTTNSLFL GSVAQGKTGE SSPRNNSNGN GNSNNIFGHE EPTISTGGGA LHSGSGGRSI VGRRVQSKID LKRNSSDDVA AVLGGSSLAN VDRTANGATR KAFSGPPSTR VPAIQGGGVG SGGDTMLHRP HPPGSGGAKL GVRPIQFALN GNSSQRLSPV PPAPPVLFDQ HKNVLEHTPF SSRQGKKGSH GSVDPFATTH ILNGVGTRLP PQLDEGGTRW GKDVSTPFAL ASVKLQQLSL PERSTSPRLA SEPPAASARC SGAVANSTKL SGSAPEDSST KSNILQYDFR VVASTGKTFN SPIKLTVGVG SLQGHRPTME DEHFVRLNAA TAAGQSVSLL GILDGHCGRR VAELASRHVP DNFVAHSALG ENNALAFVES IIQADRAIFH ALGKGTGGGA SVSCNSSGGS TLIAAAVHGR MLYVACLGDA RAVLYDGNTT IPMSEDHKPT TKREHTRILQ CGGFVQFGRV CGILAVSRAL GDYEFKFNGN RFIANRELMV SNVADVRQIN LTDSSNFLLM ACDGLWDVVE NEEATQFVRD FLSYTPDVGS SPEATKRALN NCCQKLAEFA VDRGSTDNVS VMLLFFHDVA DVVAGFDKPV GAVASPPASQ VLSPRLTGPS RGIPFGSSML DGGLHTSSGT SGTRRPSFGS VSSLHGRGRS IW

NT Sequence

atgatgttca tgtcagttag aaaaccacca ctttcttctt ccaaaggcac ggcaaaaaac cctgccttgc gcaagggtga tatggggaca gggcatgaga cctcgcaagg ttccattccg actgttccca agaccgtccc actccgcaac aacacccgat gtagcagtgg aaatcctttt ctgtacggct cgcaacgtgg tggtgcattg ccctaccaga ccaccaattc gctcttttta gggagcgttg cacagggcaa aacgggcgaa tcaagtccta gaaacaacag caatggcaat ggcaatagca ataacatctt tggacatgag gagcctacca tttccactgg tggtggggcg ctgcatagtg gaagtggcgg ccgttctatc gtagggcgac gcgtgcagtc aaaaattgat ttgaagcgta attcaagcga tgatgttgcg gctgttctcg gtggttcgag tctagcgaat gtggaccgca ccgcaaacgg cgccacacga aaagcatttt caggaccacc atcaacccgt gttccggcaa ttcaaggtgg gggagttgga agtggcgggg ataccatgct gcatcggcca catccgcctg gtagcggagg cgcgaaattg ggtgtacgcc caatacaatt tgccctgaac ggcaacagta gccaacgttt gtcgccggtc ccgcctgctc cccccgttct ttttgaccaa cacaaaaatg tattggagca caccccgttt tcctcgcgcc aggggaaaaa ggggtcacat ggaagcgtgg atccatttgc cacaacacat attctcaacg gtgtgggcac aaggcttccc cctcaattgg atgaaggagg tacacggtgg ggtaaggatg tatcgacacc gtttgcactc gcgtctgtaa aactgcagca attgtctctg ccagagagat ccacatctcc aaggttggcg tcagaaccac ctgctgcttc ggcccgctgc agtggcgctg tggcgaatag tactaaatta tcgggtagtg ctcccgaaga tagcagtacc aaaagcaata tccttcaata tgactttcgt gtagtggcta gtaccggaaa gacattcaac tctcccataa aacttacggt tggtgtgggt agtcttcaag gacaccgtcc aacgatggaa gatgagcact ttgttcgcct gaatgcagca acggcggcgg gacagtctgt gtctttgctt ggtattcttg acggtcactg cggccgtcgt gtggccgagt tagcgtcaag acatgtcccg gataattttg tcgcccactc ggcacttggt gaaaacaatg cattggcttt tgtagaatct attatacagg cagatcgagc catttttcat gctctaggta aaggtacagg cgggggagcg agtgttagtt gcaacagcag cggcggttct actcttattg ccgccgctgt tcatgggcgc atgttgtatg tagcctgttt gggagatgcc agggcagtgc tttacgatgg caacacaacc attcccatga gtgaggacca taagcccacc acaaaacggg agcacacacg tattcttcag tgtggtgggt tcgtacagtt tggtagggtg tgtggcattc ttgcggtgag tcgggcgtta ggagactatg aatttaaatt caacggcaat aggtttattg caaatcggga actgatggtg tcaaatgtgg cagatgtacg acaaatcaac ttaacagact ctagtaattt tcttttgatg gcctgtgatg gtctgtggga tgtcgtagaa aacgaggagg ccacacaatt tgttcgggac ttcttaagct acacgccaga tgtgggttca tccccggagg ctacgaagcg tgcgcttaac aactgttgcc agaaacttgc cgagtttgcc gtggatcgtg ggagcacgga caatgtctcc gtgatgttgc tatttttcca tgatgtagca gatgttgtgg ctgggtttga caagccagtg ggggcggtcg cttcacctcc ggcaagtcaa gtattgtcac cacgactcac tggcccaagt cgcggcatcc catttggtag ctccatgctt gacgggggtc tgcacacgag cagtgggaca tcaggaacga ggcgcccatc gtttggctcc gtctcctcac tgcacggccg cggtcgctct atctggtaa

Details for TrcrA.18218.b.B1.GE35206

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	77kDa
OBSERVED MW:	77kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	75
PERCENT COVERAGE:	52

Validated AA Sequence

Validated NT Sequence

ttgtttactt taagaaggag anataccatg gctcaccacc accaccacca tatgatgttc atgtcagtta gaaaaccacc actttcttct tccaaaggca cggcaaaaaa ccctgccttg cgcaagggtg atatggggac agggcatgag acctcgcaag gttccattcc gactgttccc aagaccgtcc cactccgcaa caacacccga tgtagcagtg gaaatccttt tctgtacggc tcgcaacgtg gtggtgcatt gccctaccag accaccaatt cgctcttttt agggagcgtt gcacagggca aaacgggcga atcaagtcct agaaacaaca gcaatggcaa tggcaatagc aataacatct ttggacatga ggagcctacc atttccactg gtggtggggc gctgcatagt ggaagtggcg gccgttctat cgtagggcga cgcgtgcagt caaaaattga tttgaagcgt aattcaagcg atgatgttgc ggctgttctc ggtggttcga gtctagcgaa tgtggaccgc accgcaaacg gcgccacacg aaaagcattt tcaggaccac catcaacccg tgttccggca attcaaggtg ggggagttgg aagtggcggg gataccatgc tgcatcggcc acatccgcct ggtagcggan gcgcgaaatt gggtgtacgc ccaatacaat ttgccctgaa cggcaacagt agccaacgtt tgtcgccggt cccgcctgct ccccccgttc tttttgacca acacaaaaat gtattgnagc acaccccgtt ttcctcgcgc caggggaaaa aggggtcaca tggaagcgtg gatccatttg ccacaacaca tattctcaac ggtgtgggca canngcttcc ccctcaattn gntgannngn nacacggtgg ggtaaggagt atcgacacgt ttgcactcnc gtctgtaaac tgcagcaatt gtctctncng ananancnna tnnncnnnnn nntnnnnnnn ngctnnttng ccnctgcnng nnnctntnnn annntannaa ntnnnnnnnn nnngnnnnnn aannnnnnnn nnnannnnnn nnnnnnannn nnnnnnnnnn nngnnnannn nannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tngnannnnn ncnnangnnn nnnnnnnnnn nnntnnnnnn ang

Expected Protein Sequence

MAHHHHHHMM FMSVRKPPLS SSKGTAKNPA LRKGDMGTGH ETSQGSIPTV PKTVPLRNNT RCSSGNPFLY GSQRGGALPY QTTNSLFLGS VAQGKTGESS PRNNSNGNGN SNNIFGHEEP TISTGGGALH SGSGGRSIVG RRVQSKIDLK RNSSDDVAAV LGGSSLANVD RTANGATRKA FSGPPSTRVP AIQGGGVGSG GDTMLHRPHP PGSGGAKLGV RPIQFALNGN SSQRLSPVPP APPVLFDQHK NVLEHTPFSS RQGKKGSHGS VDPFATTHIL NGVGTRLPPQ LDEGGTRWGK DVSTPFALAS VKLQQLSLPE RSTSPRLASE PPAASARCSG AVANSTKLSG SAPEDSSTKS NILQYDFRVV ASTGKTFNSP IKLTVGVGSL QGHRPTMEDE HFVRLNAATA AGQSVSLLGI LDGHCGRRVA ELASRHVPDN FVAHSALGEN NALAFVESII QADRAIFHAL GKGTGGGASV SCNSSGGSTL IAAAVHGRML YVACLGDARA VLYDGNTTIP MSEDHKPTTK REHTRILQCG GFVQFGRVCG ILAVSRALGD YEFKFNGNRF IANRELMVSN VADVRQINLT DSSNFLLMAC DGLWDVVENE EATQFVRDFL SYTPDVGSSP EATKRALNNC CQKLAEFAVD RGSTDNVSVM LLFFHDVADV VAGFDKPVGA VASPPASQVL SPRLTGPSRG IPFGSSMLDG GLHTSSGTSG TRRPSFGSVS SLHGRGRSIW

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgt tcatgtcagt tagaaaacca ccactttctt cttccaaagg cacggcaaaa aaccctgcct tgcgcaaggg tgatatgggg acagggcatg agacctcgca aggttccatt ccgactgttc ccaagaccgt cccactccgc aacaacaccc gatgtagcag tggaaatcct tttctgtacg gctcgcaacg tggtggtgca ttgccctacc agaccaccaa ttcgctcttt ttagggagcg ttgcacaggg caaaacgggc gaatcaagtc ctagaaacaa cagcaatggc aatggcaata gcaataacat ctttggacat gaggagccta ccatttccac tggtggtggg gcgctgcata gtggaagtgg cggccgttct atcgtagggc gacgcgtgca gtcaaaaatt gatttgaagc gtaattcaag cgatgatgtt gcggctgttc tcggtggttc gagtctagcg aatgtggacc gcaccgcaaa cggcgccaca cgaaaagcat tttcaggacc accatcaacc cgtgttccgg caattcaagg tgggggagtt ggaagtggcg gggataccat gctgcatcgg ccacatccgc ctggtagcgg aggcgcgaaa ttgggtgtac gcccaataca atttgccctg aacggcaaca gtagccaacg tttgtcgccg gtcccgcctg ctccccccgt tctttttgac caacacaaaa atgtattgga gcacaccccg ttttcctcgc gccaggggaa aaaggggtca catggaagcg tggatccatt tgccacaaca catattctca acggtgtggg cacaaggctt ccccctcaat tggatgaagg aggtacacgg tggggtaagg atgtatcgac accgtttgca ctcgcgtctg taaaactgca gcaattgtct ctgccagaga gatccacatc tccaaggttg gcgtcagaac cacctgctgc ttcggcccgc tgcagtggcg ctgtggcgaa tagtactaaa ttatcgggta gtgctcccga agatagcagt accaaaagca atatccttca atatgacttt cgtgtagtgg ctagtaccgg aaagacattc aactctccca taaaacttac ggttggtgtg ggtagtcttc aaggacaccg tccaacgatg gaagatgagc actttgttcg cctgaatgca gcaacggcgg cgggacagtc tgtgtctttg cttggtattc ttgacggtca ctgcggccgt cgtgtggccg agttagcgtc aagacatgtc ccggataatt ttgtcgccca ctcggcactt ggtgaaaaca atgcattggc ttttgtagaa tctattatac aggcagatcg agccattttt catgctctag gtaaaggtac aggcggggga gcgagtgtta gttgcaacag cagcggcggt tctactctta ttgccgccgc tgttcatggg cgcatgttgt atgtagcctg tttgggagat gccagggcag tgctttacga tggcaacaca accattccca tgagtgagga ccataagccc accacaaaac gggagcacac acgtattctt cagtgtggtg ggttcgtaca gtttggtagg gtgtgtggca ttcttgcggt gagtcgggcg ttaggagact atgaatttaa attcaacggc aataggttta ttgcaaatcg ggaactgatg gtgtcaaatg tggcagatgt acgacaaatc aacttaacag actctagtaa ttttcttttg atggcctgtg atggtctgtg ggatgtcgta gaaaacgagg aggccacaca atttgttcgg gacttcttaa gctacacgcc agatgtgggt tcatccccgg aggctacgaa gcgtgcgctt aacaactgtt gccagaaact tgccgagttt gccgtggatc gtgggagcac ggacaatgtc tccgtgatgt tgctattttt ccatgatgta gcagatgttg tggctgggtt tgacaagcca gtgggggcgg tcgcttcacc tccggcaagt caagtattgt caccacgact cactggccca agtcgcggca tcccatttgg tagctccatg cttgacgggg gtctgcacac gagcagtggg acatcaggaa cgaggcgccc atcgtttggc tccgtctcct cactgcacgg ccgcggtcgc tctatctggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat