TrcrA.18218.a

protein phosphatase 2C-like protein

CENTER ID: TrcrA.18218.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18218.a.B1.GE35205 full length 1 347
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18218.a.B1.PS01533 full length 1 347 unavailable

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053506659.39
RefSeq: XP_809480.1
UniProt: Q4D524

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMFMSVRKPP LSSSKGTAKN PALRKGDVGT GNETSQGSIP TVPKTVPLRN NTRCSSGNPF LYGSQRGGAL PYQTTNSLFL GSVAQGKTGE SSPRNSSNGN GNSNNIFGHE EPTIPTSGGA LHSGSGGRSI VGRRMQSKID LKRNSSDNVA AVLGGSSLAS VDRTANGATR KAFSGPPSTR VPAIQGGGAG SGGDTMLHRP HPPGSGGAKL GVRPIQFALN GNSSQRLSPV PPVPPALFDQ HKNVLEPTPF SSRQGKKGSH GSVDPFATTH ILNGVGTRLP PQLDEGGTRW GKDVSTPFAL ASVKLQQLSL PERSTSPRLA SEPPAASARC SGAVTNSTKL SGSAPED
NT Sequence
atgatgttca tgtcagttag aaaaccacca ctttcttctt ccaaaggcac ggcaaaaaac cctgccttgc gcaagggtga tgtggggaca gggaatgaga cctcgcaagg ttccattccg actgttccca agaccgtccc actccgcaac aacacccgat gtagcagtgg aaatcctttt ctgtacggct cgcaacgtgg tggtgcattg ccctaccaga ccaccaattc gctcttttta gggagcgttg cacagggtaa aacaggcgaa tcaagtccta gaaatagcag caatggcaat ggcaatagca ataacatctt tggacatgag gagcctacca ttcccactag tggtggggcg ctgcatagtg gaagtggcgg tcgttctatc gtagggcgac gcatgcagtc aaaaattgat ttgaagcgta attcaagcga taatgttgcg gccgttctcg gtggttcgag tctagcgagt gtggaccgca ccgcaaacgg cgccacacga aaagcatttt caggaccacc atcaacccgt gttccggcaa ttcaaggtgg gggagctgga agtggcgggg ataccatgct gcatcggcca catccgcctg gtagcggagg cgcgaaattg ggtgtacgcc caatacaatt tgccctgaac ggcaacagta gccaacgttt gtcgccggtc ccacctgttc cccccgctct gtttgaccaa cacaaaaatg tattggagcc caccccgttt tcctcgcgcc aggggaaaaa ggggtcacat ggaagcgtgg atccatttgc cacaacacat attctcaacg gtgtaggcac aaggcttccc cctcaattgg atgaaggagg tacacggtgg ggtaaggatg tatcgacacc gtttgcactc gcgtctgtga aactgcagca attgtctctg ccagagagat ccacatctcc aaggttggcg tcagaaccac ctgctgcttc ggcccgctgc agtggcgccg tgacgaatag tactaaatta tcgggtagtg ctcccgaaga t
Details for TrcrA.18218.a.B1.GE35205
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 36kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMM FMSVRKPPLS SSKGTAKNPA LRKGDVGTGN ETSQGSIPTV PKTVPLRNNT RCSSGNPFLY GSQRGGALPY QTTNSLFLGS VAQGKTGESS PRNSSNGNGN SNNIFGHEEP TIPTSGGALH SGSGGRSIVG RRMQSKIDLK RNSSDNVAAV LGGSSLASVD RTANGATRKA FSGPPSTRVP AIQGGGAGSG GDTMLHRPHP PGSGGAKLGV RPIQFALNGN SSQRLSPVPP VPPALFDQHK NVLEPTPFSS RQGKKGSHGS VDPFATTHIL NGVGTRLPPQ LDEGGTRWGK DVSTPFALAS VKLQQLSLPE RSTSPRLASE PPAASARCSG AVTNSTKLSG SA
Validated NT Sequence
ggagcactac ccgataattt agtactattc gtcacggcgc cactgcagcg ggccgaagca gcaggtggtt ctgacgccaa ccttggagat gtggatctct ctggcagaga caattgctgc agtttcacag acgcgagtgc aaacggtgtc gatacatcct taccccaccg tgtacctcct tcatccaatt gagggggaag ccttgtgcct acaccgttga gaatatgtgt tgtggcaaat ggatccacgc ttccatgtga cccctttttc ccctggcgcg aggaaaacgg ggtgggctcc aatacatttt tgtgttggtc aaacagagcg gggggaacag gtgggaccgg cgacaaacgt tggctactgt tgccgttcag ggcaaattgt attgggcgta cacccaattt cgcgcctccg ctaccaggcg gatgtggccg atgcagcatg gtatccccgc cacttccagc tcccccacct tgaattgccg gaacacgggt tgatggtggt cctgaaaatg cttttcgtgt ggcgccgttt gcggtgcggt ccacactcgc tagactcgaa ccaccgagaa cggccgcaac attatcgctt gaattacgct tcaaatcaat ttttgactgc atgcgtcgcc ctacgataga acgaccgcca cttccactat gcagcgcccc accactagtg ggaatggtag gctcctcatg tccaaagatg ttattgctat tgccattgcc attgctgcta tttctaggac ttgattcgcc tgttttaccc tgtgcaacgc tccctaaaaa gagcgaattg gtggtctggt agggcaatgc accaccacgt tgcgagccgt acagaaaagg atttccactg ctacatcggg tgttgttgcg gagtgggacg gtcttgggaa cagtcggaat ggaaccttgc gaggtctcat tccctgtccc cacatcaccc ttgcgcaagg cagggttttt tgccgtgcct ttggaagaag aaagtggtgg ttttctaact gacatgaaca tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMM FMSVRKPPLS SSKGTAKNPA LRKGDVGTGN ETSQGSIPTV PKTVPLRNNT RCSSGNPFLY GSQRGGALPY QTTNSLFLGS VAQGKTGESS PRNSSNGNGN SNNIFGHEEP TIPTSGGALH SGSGGRSIVG RRMQSKIDLK RNSSDNVAAV LGGSSLASVD RTANGATRKA FSGPPSTRVP AIQGGGAGSG GDTMLHRPHP PGSGGAKLGV RPIQFALNGN SSQRLSPVPP VPPALFDQHK NVLEPTPFSS RQGKKGSHGS VDPFATTHIL NGVGTRLPPQ LDEGGTRWGK DVSTPFALAS VKLQQLSLPE RSTSPRLASE PPAASARCSG AVTNSTKLSG SAPED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgt tcatgtcagt tagaaaacca ccactttctt cttccaaagg cacggcaaaa aaccctgcct tgcgcaaggg tgatgtgggg acagggaatg agacctcgca aggttccatt ccgactgttc ccaagaccgt cccactccgc aacaacaccc gatgtagcag tggaaatcct tttctgtacg gctcgcaacg tggtggtgca ttgccctacc agaccaccaa ttcgctcttt ttagggagcg ttgcacaggg taaaacaggc gaatcaagtc ctagaaatag cagcaatggc aatggcaata gcaataacat ctttggacat gaggagccta ccattcccac tagtggtggg gcgctgcata gtggaagtgg cggtcgttct atcgtagggc gacgcatgca gtcaaaaatt gatttgaagc gtaattcaag cgataatgtt gcggccgttc tcggtggttc gagtctagcg agtgtggacc gcaccgcaaa cggcgccaca cgaaaagcat tttcaggacc accatcaacc cgtgttccgg caattcaagg tgggggagct ggaagtggcg gggataccat gctgcatcgg ccacatccgc ctggtagcgg aggcgcgaaa ttgggtgtac gcccaataca atttgccctg aacggcaaca gtagccaacg tttgtcgccg gtcccacctg ttccccccgc tctgtttgac caacacaaaa atgtattgga gcccaccccg ttttcctcgc gccaggggaa aaaggggtca catggaagcg tggatccatt tgccacaaca catattctca acggtgtagg cacaaggctt ccccctcaat tggatgaagg aggtacacgg tggggtaagg atgtatcgac accgtttgca ctcgcgtctg tgaaactgca gcaattgtct ctgccagaga gatccacatc tccaaggttg gcgtcagaac cacctgctgc ttcggcccgc tgcagtggcg ccgtgacgaa tagtactaaa ttatcgggta gtgctcccga agattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for TrcrA.18218.a.B1.PS01533
PURIFICATION DATe: 6/21/2012
CONCENTRATION: 23.3mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMM FMSVRKPPLS SSKGTAKNPA LRKGDVGTGN ETSQGSIPTV PKTVPLRNNT RCSSGNPFLY GSQRGGALPY QTTNSLFLGS VAQGKTGESS PRNSSNGNGN SNNIFGHEEP TIPTSGGALH SGSGGRSIVG RRMQSKIDLK RNSSDNVAAV LGGSSLASVD RTANGATRKA FSGPPSTRVP AIQGGGAGSG GDTMLHRPHP PGSGGAKLGV RPIQFALNGN SSQRLSPVPP VPPALFDQHK NVLEPTPFSS RQGKKGSHGS VDPFATTHIL NGVGTRLPPQ LDEGGTRWGK DVSTPFALAS VKLQQLSLPE RSTSPRLASE PPAASARCSG AVTNSTKLSG SA
Validated NT Sequence
ggagcactac ccgataattt agtactattc gtcacggcgc cactgcagcg ggccgaagca gcaggtggtt ctgacgccaa ccttggagat gtggatctct ctggcagaga caattgctgc agtttcacag acgcgagtgc aaacggtgtc gatacatcct taccccaccg tgtacctcct tcatccaatt gagggggaag ccttgtgcct acaccgttga gaatatgtgt tgtggcaaat ggatccacgc ttccatgtga cccctttttc ccctggcgcg aggaaaacgg ggtgggctcc aatacatttt tgtgttggtc aaacagagcg gggggaacag gtgggaccgg cgacaaacgt tggctactgt tgccgttcag ggcaaattgt attgggcgta cacccaattt cgcgcctccg ctaccaggcg gatgtggccg atgcagcatg gtatccccgc cacttccagc tcccccacct tgaattgccg gaacacgggt tgatggtggt cctgaaaatg cttttcgtgt ggcgccgttt gcggtgcggt ccacactcgc tagactcgaa ccaccgagaa cggccgcaac attatcgctt gaattacgct tcaaatcaat ttttgactgc atgcgtcgcc ctacgataga acgaccgcca cttccactat gcagcgcccc accactagtg ggaatggtag gctcctcatg tccaaagatg ttattgctat tgccattgcc attgctgcta tttctaggac ttgattcgcc tgttttaccc tgtgcaacgc tccctaaaaa gagcgaattg gtggtctggt agggcaatgc accaccacgt tgcgagccgt acagaaaagg atttccactg ctacatcggg tgttgttgcg gagtgggacg gtcttgggaa cagtcggaat ggaaccttgc gaggtctcat tccctgtccc cacatcaccc ttgcgcaagg cagggttttt tgccgtgcct ttggaagaag aaagtggtgg ttttctaact gacatgaaca tcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMM FMSVRKPPLS SSKGTAKNPA LRKGDVGTGN ETSQGSIPTV PKTVPLRNNT RCSSGNPFLY GSQRGGALPY QTTNSLFLGS VAQGKTGESS PRNSSNGNGN SNNIFGHEEP TIPTSGGALH SGSGGRSIVG RRMQSKIDLK RNSSDNVAAV LGGSSLASVD RTANGATRKA FSGPPSTRVP AIQGGGAGSG GDTMLHRPHP PGSGGAKLGV RPIQFALNGN SSQRLSPVPP VPPALFDQHK NVLEPTPFSS RQGKKGSHGS VDPFATTHIL NGVGTRLPPQ LDEGGTRWGK DVSTPFALAS VKLQQLSLPE RSTSPRLASE PPAASARCSG AVTNSTKLSG SAPED
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATGT TCATGTCAGT TAGAAAACCA CCACTTTCTT CTTCCAAAGG CACGGCAAAA AACCCTGCCT TGCGCAAGGG TGATGTGGGG ACAGGGAATG AGACCTCGCA AGGTTCCATT CCGACTGTTC CCAAGACCGT CCCACTCCGC AACAACACCC GATGTAGCAG TGGAAATCCT TTTCTGTACG GCTCGCAACG TGGTGGTGCA TTGCCCTACC AGACCACCAA TTCGCTCTTT TTAGGGAGCG TTGCACAGGG TAAAACAGGC GAATCAAGTC CTAGAAATAG CAGCAATGGC AATGGCAATA GCAATAACAT CTTTGGACAT GAGGAGCCTA CCATTCCCAC TAGTGGTGGG GCGCTGCATA GTGGAAGTGG CGGTCGTTCT ATCGTAGGGC GACGCATGCA GTCAAAAATT GATTTGAAGC GTAATTCAAG CGATAATGTT GCGGCCGTTC TCGGTGGTTC GAGTCTAGCG AGTGTGGACC GCACCGCAAA CGGCGCCACA CGAAAAGCAT TTTCAGGACC ACCATCAACC CGTGTTCCGG CAATTCAAGG TGGGGGAGCT GGAAGTGGCG GGGATACCAT GCTGCATCGG CCACATCCGC CTGGTAGCGG AGGCGCGAAA TTGGGTGTAC GCCCAATACA ATTTGCCCTG AACGGCAACA GTAGCCAACG TTTGTCGCCG GTCCCACCTG TTCCCCCCGC TCTGTTTGAC CAACACAAAA ATGTATTGGA GCCCACCCCG TTTTCCTCGC GCCAGGGGAA AAAGGGGTCA CATGGAAGCG TGGATCCATT TGCCACAACA CATATTCTCA ACGGTGTAGG CACAAGGCTT CCCCCTCAAT TGGATGAAGG AGGTACACGG TGGGGTAAGG ATGTATCGAC ACCGTTTGCA CTCGCGTCTG TGAAACTGCA GCAATTGTCT CTGCCAGAGA GATCCACATC TCCAAGGTTG GCGTCAGAAC CACCTGCTGC TTCGGCCCGC TGCAGTGGCG CCGTGACGAA TAGTACTAAA TTATCGGGTA GTGCTCCCGA AGATTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT