SSGCID: Target Page

TrcrA.18217.a

DNA topoisomerase type IB small subunit

CENTER ID:	TrcrA.18217.a
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.18217.a.B1.GE35204	full length		1	272

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053506625.110
RefSeq:	XP_817976.1
UniProt:	Q4DUD0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRKKERSEMT EVTPLKVPLA TGAPTPPASY PPTKVPVKMP YVLPKPPFAP VVPMRPPAPR LKRDGDDGDL AVPGIADASK RSRVEDRERR GRAMSESSNS ESSSNSDSDD GSDSDSHSSS SGSSGDGESF SSSTEGSGSD ETRSGDELTL FEIAKARGLV DERVDNNNNN NEEEEEVAPV GPPPRPPIVR SFPNNVDLAL QRYEERLNRE ENMIRIKDDN KAVSLGTSKV NYIDPRIVCS WAKEYNVPIG KIFSATLQKK FPWAMGAENF SF

NT Sequence

atgaggaaaa aagaaagaag tgaaatgact gaagtgacgc cgctgaaagt cccactcgct acgggggctc caacgccgcc ggcttcctat cctcctacaa aggtgccggt aaaaatgcct tacgtgttgc ccaagccacc gtttgcaccg gtggtgccaa tgaggccacc cgcaccgagg ctcaaaagag acggcgatga tggtgacttg gcggtcccag gaattgcaga cgcctccaag aggtcccggg tggaggatcg cgaacggcgg ggccgagcga tgtcggaaag cagcaacagc gagagcagca gcaacagtga tagtgatgat ggcagcgaca gcgacagtca cagtagcagc agtggaagca gtggggatgg tgaaagtttt tcttcgtcta cagaaggttc cggcagcgac gaaacgcgtt ctggtgatga actcactctg tttgagattg cgaaggcaag gggtcttgtc gatgaacgcg tcgataataa taataataat aatgaggagg aggaggaagt ggcacctgtt gggccgccac cgcgtccgcc aattgtgcgc tcctttccaa ataatgtgga tttggctctg cagcgctacg aagagcgcct aaatcgtgag gagaacatga ttcgcattaa ggatgacaat aaggctgtct ctctaggcac cagcaaggtc aactacatcg acccccgcat cgtgtgttca tgggcaaaag aatacaacgt acccataggg aaaatatttt ccgcaacgct tcaaaaaaaa ttcccgtggg cgatgggggc ggagaatttt tccttttga

Details for TrcrA.18217.a.B1.GE35204

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	30kDa
OBSERVED MW:	17kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR KKERSEMTEV TPLKVPLATG APTPPASYPP TKVPVKMPYV LPKPPFAPVV PMRPPAPRLK RDGDDGDLAV PGIADASKRS RVEDRERRGR AMSESSNSES SSNSDSDDGS DSDSHSSSSG SSGDGESFSS STEGSGSDET RSGDELTLFE IAKARGLVDE RVDNNNNNNE EEEEVAPVGP PPRPPIVRSF PNNVDLALQR YEERLNREEN MIRIKDDNKA VSLGTSKVNY IDPRIVCSWA KEYNVPIGKI FSATLQKKFP WAMGAENFSF

Validated NT Sequence

ctatcttact cacttaaaag gaaaaattct ccgcccccat cgcccacggg aatttttttt gaagcgttgc ggaaaatatt ttccctatgg gtacgttgta ttcttttgcc catgaacaca cgatgcgggg gtcgatgtag ttgaccttgc tggtgcctag agagacagcc ttattgtcat ccttaatgcg aatcatgttc tcctcacgat ttaggcgctc ttcgtagcgc tgcagagcca aatccacatt atttggaaag gagcgcacaa ttggcggacg cggtggcggc ccaacaggtg ccacttcctc ctcctcctca ttattattat tattattatc gacgcgttca tcgacaagac cccttgcctt cgcaatctca aacagagtga gttcatcacc agaacgcgtt tcgtcgctgc cggaaccttc tgtagacgaa gaaaaacttt caccatcccc actgcttcca ctgctgctac tgtgactgtc gctgtcgctg ccatcatcac tatcactgtt gctgctgctc tcgctgttgc tgctttccga catcgctcgg ccccgccgtt cgcgatcctc cacccgggac ctcttggagg cgtctgcaat tcctgggacc gccaagtcac catcatcgcc gtctcttttg agcctcggtg cgggtggcct cattggcacc accggtgcaa acggtggctt gggcaacacg taaggcattt ttaccggcac ctttgtagga ggataggaag ccggcggcgt tggagccccc gtagcgagtg ggactttcag cggcgtcact tcagtcattt cacttctttc ttttttcctc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagga aaaaagaaag aagtgaaatg actgaagtga cgccgctgaa agtcccactc gctacggggg ctccaacgcc gccggcttcc tatcctccta caaaggtgcc ggtaaaaatg ccttacgtgt tgcccaagcc accgtttgca ccggtggtgc caatgaggcc acccgcaccg aggctcaaaa gagacggcga tgatggtgac ttggcggtcc caggaattgc agacgcctcc aagaggtccc gggtggagga tcgcgaacgg cggggccgag cgatgtcgga aagcagcaac agcgagagca gcagcaacag tgatagtgat gatggcagcg acagcgacag tcacagtagc agcagtggaa gcagtgggga tggtgaaagt ttttcttcgt ctacagaagg ttccggcagc gacgaaacgc gttctggtga tgaactcact ctgtttgaga ttgcgaaggc aaggggtctt gtcgatgaac gcgtcgataa taataataat aataatgagg aggaggagga agtggcacct gttgggccgc caccgcgtcc gccaattgtg cgctcctttc caaataatgt ggatttggct ctgcagcgct acgaagagcg cctaaatcgt gaggagaaca tgattcgcat taaggatgac aataaggctg tctctctagg caccagcaag gtcaactaca tcgacccccg catcgtgtgt tcatgggcaa aagaatacaa cgtacccata gggaaaatat tttccgcaac gcttcaaaaa aaattcccgt gggcgatggg ggcggagaat ttttcctttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat