TrcrA.18192.a

25 kDa elongation factor 1-beta (EF-1-beta)

CENTER ID: TrcrA.18192.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18192.a.B1.GE36373 full length 1 222
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18192.a.B1.PS01719 full length 1 222

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TCSYLVIO_003973
UniProt: P34827

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVKDVNKRS GELEGKLKGK LFLGGTKPSK EDVKLFNDLL GAENTSLYLW VKHMTSFTEA ERKAWGAPVK VTATTSASAP AKQAPKKAAS APAKQADEDE EIDLFGEATE EETAALEAKK KKDTDAKKAK KEVIAKSSIL FDVKPWDDTV DLQALANKLH AVKRDGLLWG DHKLVPVAFG VKKLQQLIVI EDDKVLSDDL EELIMSFEDE VQSMDIVAWN KI
NT Sequence
atgtctgtta aggacgtcaa caagaggagc ggtgagctgg agggcaagct gaagggcaag ctttttttgg gcggcacaaa gccttccaaa gaggacgtga agctgttcaa cgacttgctg ggcgccgaaa acacatcctt gtacctgtgg gtgaagcata tgacctcctt cacggaggcg gagcgcaagg cgtggggggc ccctgtgaag gttacagcta ccacttcggc ttccgctccc gcaaagcagg caccaaagaa ggcggcgtct gcccccgcca agcaagccga tgaagacgag gaaattgacc tcttcgggga agcgacggag gaggagacgg ctgctcttga ggccaagaag aagaaggaca ctgacgccaa gaaggcaaag aaggaggtta tcgccaaatc ctctatcctg ttcgacgtca agccgtggga cgacaccgtg gatttgcagg ctctggcgaa taagctgcat gccgttaagc gcgacggcct gctgtggggc gaccacaagc tggtgcctgt ggcattcggc gtcaagaagc tgcagcagct tatcgtcatt gaggacgaca aggtcctcag tgatgatttg gaggaattga tcatgtcgtt tgaggacgaa gtgcagtcca tggacatcgt tgcctggaat aagatctga
Details for TrcrA.18192.a.B1.GE36373
HARVESTED ON: 10/23/2012
SEQUENCED ON: 10/26/2012
EXPECTED MW: 25kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 96
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS VKDVNKRSGE LEGKLKGKLF LGGTKPSQED VKLFNELLGA ENTSLYRWVK HMASFTEAER KAWGAPVKVT ATTSASAPAK QTPKKAASAP AKQADEDEDI DLFGEATEEE TAALEAKKKK DADAKKAKKE VIAKSSILFD VKPWDDTVDL QALANKLHAV KRDGLLWGDH KLVPVAFGVK KLQQLIVIED DKVSSDDLEE LIMSFEDEVQ SMDIVAWNKI
Validated NT Sequence
atcctatctt actcacttag atcttattcc aggcaacgat gtccatggac tgcacttcgt cctcaaacga catgatcaat tcctccaaat catcactgga gaccttgtcg tcctcaatga caataagctg ctgcagcttc ttcacgccga atgccacagg caccagcttg tgatcgcccc acagcaggcc gtcgcgctta acggcgtgca gcttattcgc cagagcctgc aaatccacgg tgtcatccca cggcttgacg tcgaacagga tggaggattt ggcgataacc tccttctttg ccttcttggc gtcagcgtcc ttcttcttct tggcctcaag agcagccgtc tcctcctccg tcgcttcccc gaagaggtca atgtcttcgt cttcatcggc ctgcttggcg ggggcagacg ccgccttctt tggtgtctgc tttgcgggag cggaagccga agtggtagct gtcaccttca caggggcccc ccacgccttg cgctccgcct ccgtgaagga tgccatatgc ttcacccaac ggtacaaaga tgtgttttcg gcgcccagca attcgttgaa cagcttcacg tcctcttggg aaggctttgt gccgcccaaa aaaagcttgc ccttcagctt gccctccagc tcaccgctcc tcttgttgac gtccttaaca gacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMS VKDVNKRSGE LEGKLKGKLF LGGTKPSKED VKLFNDLLGA ENTSLYLWVK HMTSFTEAER KAWGAPVKVT ATTSASAPAK QAPKKAASAP AKQADEDEEI DLFGEATEEE TAALEAKKKK DTDAKKAKKE VIAKSSILFD VKPWDDTVDL QALANKLHAV KRDGLLWGDH KLVPVAFGVK KLQQLIVIED DKVLSDDLEE LIMSFEDEVQ SMDIVAWNKI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg ttaaggacgt caacaagagg agcggtgagc tggagggcaa gctgaagggc aagctttttt tgggcggcac aaagccttcc aaagaggacg tgaagctgtt caacgacttg ctgggcgccg aaaacacatc cttgtacctg tgggtgaagc atatgacctc cttcacggag gcggagcgca aggcgtgggg ggcccctgtg aaggttacag ctaccacttc ggcttccgct cccgcaaagc aggcaccaaa gaaggcggcg tctgcccccg ccaagcaagc cgatgaagac gaggaaattg acctcttcgg ggaagcgacg gaggaggaga cggctgctct tgaggccaag aagaagaagg acactgacgc caagaaggca aagaaggagg ttatcgccaa atcctctatc ctgttcgacg tcaagccgtg ggacgacacc gtggatttgc aggctctggc gaataagctg catgccgtta agcgcgacgg cctgctgtgg ggcgaccaca agctggtgcc tgtggcattc ggcgtcaaga agctgcagca gcttatcgtc attgaggacg acaaggtcct cagtgatgat ttggaggaat tgatcatgtc gtttgaggac gaagtgcagt ccatggacat cgttgcctgg aataagatct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for TrcrA.18192.a.B1.PS01719
PURIFICATION DATe: 1/9/2013
CONCENTRATION: 42.5mg/ml
OBSERVED MW: 31kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS VKDVNKRSGE LEGKLKGKLF LGGTKPSQED VKLFNELLGA ENTSLYRWVK HMASFTEAER KAWGAPVKVT ATTSASAPAK QTPKKAASAP AKQADEDEDI DLFGEATEEE TAALEAKKKK DADAKKAKKE VIAKSSILFD VKPWDDTVDL QALANKLHAV KRDGLLWGDH KLVPVAFGVK KLQQLIVIED DKVSSDDLEE LIMSFEDEVQ SMDIVAWNKI
Validated NT Sequence
atcctatctt actcacttag atcttattcc aggcaacgat gtccatggac tgcacttcgt cctcaaacga catgatcaat tcctccaaat catcactgga gaccttgtcg tcctcaatga caataagctg ctgcagcttc ttcacgccga atgccacagg caccagcttg tgatcgcccc acagcaggcc gtcgcgctta acggcgtgca gcttattcgc cagagcctgc aaatccacgg tgtcatccca cggcttgacg tcgaacagga tggaggattt ggcgataacc tccttctttg ccttcttggc gtcagcgtcc ttcttcttct tggcctcaag agcagccgtc tcctcctccg tcgcttcccc gaagaggtca atgtcttcgt cttcatcggc ctgcttggcg ggggcagacg ccgccttctt tggtgtctgc tttgcgggag cggaagccga agtggtagct gtcaccttca caggggcccc ccacgccttg cgctccgcct ccgtgaagga tgccatatgc ttcacccaac ggtacaaaga tgtgttttcg gcgcccagca attcgttgaa cagcttcacg tcctcttggg aaggctttgt gccgcccaaa aaaagcttgc ccttcagctt gccctccagc tcaccgctcc tcttgttgac gtccttaaca gacatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMS VKDVNKRSGE LEGKLKGKLF LGGTKPSKED VKLFNDLLGA ENTSLYLWVK HMTSFTEAER KAWGAPVKVT ATTSASAPAK QAPKKAASAP AKQADEDEEI DLFGEATEEE TAALEAKKKK DTDAKKAKKE VIAKSSILFD VKPWDDTVDL QALANKLHAV KRDGLLWGDH KLVPVAFGVK KLQQLIVIED DKVLSDDLEE LIMSFEDEVQ SMDIVAWNKI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTG TTAAGGACGT CAACAAGAGG AGCGGTGAGC TGGAGGGCAA GCTGAAGGGC AAGCTTTTTT TGGGCGGCAC AAAGCCTTCC AAAGAGGACG TGAAGCTGTT CAACGACTTG CTGGGCGCCG AAAACACATC CTTGTACCTG TGGGTGAAGC ATATGACCTC CTTCACGGAG GCGGAGCGCA AGGCGTGGGG GGCCCCTGTG AAGGTTACAG CTACCACTTC GGCTTCCGCT CCCGCAAAGC AGGCACCAAA GAAGGCGGCG TCTGCCCCCG CCAAGCAAGC CGATGAAGAC GAGGAAATTG ACCTCTTCGG GGAAGCGACG GAGGAGGAGA CGGCTGCTCT TGAGGCCAAG AAGAAGAAGG ACACTGACGC CAAGAAGGCA AAGAAGGAGG TTATCGCCAA ATCCTCTATC CTGTTCGACG TCAAGCCGTG GGACGACACC GTGGATTTGC AGGCTCTGGC GAATAAGCTG CATGCCGTTA AGCGCGACGG CCTGCTGTGG GGCGACCACA AGCTGGTGCC TGTGGCATTC GGCGTCAAGA AGCTGCAGCA GCTTATCGTC ATTGAGGACG ACAAGGTCCT CAGTGATGAT TTGGAGGAAT TGATCATGTC GTTTGAGGAC GAAGTGCAGT CCATGGACAT CGTTGCCTGG AATAAGATCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT