TrcrA.18143.a

phosphoglucomutase, putative

CENTER ID: TrcrA.18143.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.18143.a.B1.GE35202 full length 1 600
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053508637.90
RefSeq: XP_815237.1
UniProt: Q4DLI9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLGETQCVV FQIMLAVKKV PTRPFIDQQP GTSGLRKKVR VFQQENYLAN FIQSTFNAIG KQGMIPDTLV LGGDGRYFLS EAIQIIIKLA AANGVSNVWV GKDGLLSTPA VSNIIRQRRD GDVTAKGAFI LTASHNPGGP EEDFGIKYNT ENGGPAAEKI TSAIYEETLK IDHFLTCPNI GTVNVSEMGD HLFERFRVSV IHSTEDYVQS MKKIFDFQSI RNLLNRTDFT IRLDGLSGIG GPYMKDIFVS ALGVPESALC GATPLPDFGK QHPDPNLTYA KELVRTMGLD STGRPVADFV GEVPNFAAAF DGDADRNMIL GERFFVTPSD SLAILSANAN VVPFFAQQGG IKAVARSMPT SGAVDRVAEM HHLKIFEVPT GWKFFGNLMD SRELFGGEDY NPLICGEESF GTGSNHIREK DGVWAALFWL SVIASKNVDP SKPLVGVKDI VEDHWTRYGR NYYCRYDYEN VAEDSAKAVM ETVQRQRPQD IPSLQGKRCV KVDNFEYHDP VDGLVSKNQG IRVIFEDGSR FVIRLSGTGS SGATIRLYLE HYMEPNAVAR HIRDGTLPTP QSALANLIAV ALNVSQISEL TGRDAPTVIT
NT Sequence
atgaatctgg gcgaaaccca gtgtgtagtg tttcaaataa tgctcgccgt gaaaaaagtg ccaacaaggc cctttattga tcaacaacca ggaacgtcag gacttcgcaa gaaggtgagg gtgttccagc aagaaaacta tctagccaac tttattcaaa gcacttttaa tgccattggg aaacagggga tgattcccga tacccttgtc cttggtggtg atgggcggta ctttctctct gaggctattc agatcattat caaattggct gccgccaatg gggtatcaaa tgtttgggtg ggtaaagatg gtcttctctc aacccctgcc gtgtcaaaca tcatccgtca acgaagggat ggtgatgtca cggcgaaggg ggcctttatt ctcacagcaa gtcataatcc cggtggccct gaggaagatt ttggtatcaa gtacaataca gaaaatggag gacctgcggc tgagaaaatt acttctgcta tttacgaaga gaccttgaaa attgatcatt ttctgacctg cccaaatatt ggcacagtga acgtgtcaga aatgggtgac catttatttg agaggttcag agtttcggta atccatagca cagaggatta cgtccagagc atgaagaaaa tctttgactt tcaaagtatc cgaaatctgt taaatagaac tgattttacg attcggttgg atggcttaag tggtattggg ggcccttata tgaaggatat ttttgtttct gccttgggcg ttccagaaag tgctttgtgc ggtgctacgc cattgccgga ctttggtaag caacatccgg atccaaacct cacatatgcg aaggagcttg tgagaaccat gggacttgat tccaccggca ggcctgttgc tgactttgtt ggcgaggtgc cgaactttgc tgcggcattt gatggggatg cagatcgtaa tatgattctt ggcgagcgct tttttgtaac accgtctgat tctttggcaa ttctttcagc aaatgcaaat gtcgtgccat tttttgctca acaaggtgga attaaggcgg tagcgagatc tatgccgacg agtggagctg ttgaccgtgt tgcagaaatg catcacctta aaatatttga agttcccaca ggttggaagt tttttgggaa tctgatggac agccgggagt tatttggagg cgaagattac aatcctctca tttgtggaga ggaaagcttt ggtactggta gcaaccatat tcgtgagaag gatggcgtgt gggcagcatt attttggcta tcggtaattg cgagtaaaaa tgtggatccg tcaaagcctc ttgtgggtgt aaaagacatt gttgaagacc actggacgcg ctacgggcgg aactactact gtcgatacga ctatgaaaat gtggcagagg attcagccaa ggctgtgatg gagactgtac agaggcagcg cccccaggac attccctcct tgcagggaaa acgctgtgtc aaggttgaca actttgagta ccacgaccct gtagacggct tggtctccaa aaatcagggc attcgtgtga tatttgagga tggttctcga tttgtgattc gtttaagcgg aactggatcg tcaggcgcca caattcgact atacctggag cattacatgg agcccaatgc cgtagcacgg catatccgtg acggcacgct gcctactcct caatctgcgc tggccaatct catcgccgtt gcgttgaatg tctcacagat ttcggagctt acggggcgtg acgcccccac agtcatcact tga
Details for TrcrA.18143.a.B1.GE35202
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 48
Validated AA Sequence
MAHHHHHHMN LGETQCVVFQ IMLAVKKVPT RPFIDQQPGT SGLRKKVRVF QQENYLANFI QSTFNAIGKQ GMIPDTLVLG GDGRYFLSEA IQIIIKLAAA NGVSNVWVGK DGLLSTPAVS NIIRQRRDGD VTAKGAFILT ASHNPGGPEE DFGIKYNTEN GGPAAEKITS AIYEETLKID HFLTCPNIGT VNVSEMGDHL FERFRVSVIH STEDYVQSMK KIFDFQSIRN LLNRTDFTIR LDGLSGIGGP YMKDIFVSAL GVPESALCGA TPLPDFGKQH PDPNLTYAXX XCENHGT
Validated NT Sequence
ttttntttac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc tgggcgaaac ccagtgtgta gtgtttcaaa taatgctcgc cgtgaaaaaa gtgccaacaa ggccctttat tgatcaacaa ccaggaacgt caggacttcg caagaaggtg agggtgttcc agcaagaaaa ctatctagcc aactttattc aaagcacttt taatgccatt gggaaacagg ggatgattcc cgataccctt gtccttggtg gtgatgggcg gtactttctc tctgaggcta ttcagatcat tatcaaattg gctgccgcca atggggtatc aaatgtttgg gtgggtaaag atggtcttct ctcaacccct gccgtgtcaa acatcatccg tcaacgaagg gatggtgatg tcacggcgaa gggggccttt attctcacag caagtcataa tcccggtggc cctgaggaag attttggtat caagtacaat acagaaaatg gaggacctgc ggctgagaaa attacttctg ctatttacga agagaccttg aaaattgatc attttctgac ctgcccaaat attggcacag tgaacgtgtc agaaatgggt gaccatttat ttgagaggtt cagagtttcg gtaatccata gcacagagga ttacgtccag agcatgaaga aaatctttga ctttcaaagt atccgaaatc tgttaaatag aactgatttt acgattcggt tggatggctt aagtggtatt gggggccctt atatgaagga tatttttgtt tctgccttgg gcgttccaga aagtgctttg tgcggtgcta cgccnttgcc ggactttggt aagcaacatc cggatccaaa cctcacatat gcnaangnan cttgtgagaa ccatgggact tgattccacc ngcnggcctg ttgctgactt tgttggcnag gtgccgaact ttgctgnngc atttgatggg gatgcngatc gtaatatgat ncnnnncgag cgcttttann nncnnnctgn nttnnttngn nnntnntttn ngcnnngnnn nannnnnnnt tttttnnnnn nnnnnnnnnn nntnnnnnnn nnnnnnnnna annntnnccn nnnannnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaannn nnnc
Expected Protein Sequence
MAHHHHHHMN LGETQCVVFQ IMLAVKKVPT RPFIDQQPGT SGLRKKVRVF QQENYLANFI QSTFNAIGKQ GMIPDTLVLG GDGRYFLSEA IQIIIKLAAA NGVSNVWVGK DGLLSTPAVS NIIRQRRDGD VTAKGAFILT ASHNPGGPEE DFGIKYNTEN GGPAAEKITS AIYEETLKID HFLTCPNIGT VNVSEMGDHL FERFRVSVIH STEDYVQSMK KIFDFQSIRN LLNRTDFTIR LDGLSGIGGP YMKDIFVSAL GVPESALCGA TPLPDFGKQH PDPNLTYAKE LVRTMGLDST GRPVADFVGE VPNFAAAFDG DADRNMILGE RFFVTPSDSL AILSANANVV PFFAQQGGIK AVARSMPTSG AVDRVAEMHH LKIFEVPTGW KFFGNLMDSR ELFGGEDYNP LICGEESFGT GSNHIREKDG VWAALFWLSV IASKNVDPSK PLVGVKDIVE DHWTRYGRNY YCRYDYENVA EDSAKAVMET VQRQRPQDIP SLQGKRCVKV DNFEYHDPVD GLVSKNQGIR VIFEDGSRFV IRLSGTGSSG ATIRLYLEHY MEPNAVARHI RDGTLPTPQS ALANLIAVAL NVSQISELTG RDAPTVIT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc tgggcgaaac ccagtgtgta gtgtttcaaa taatgctcgc cgtgaaaaaa gtgccaacaa ggccctttat tgatcaacaa ccaggaacgt caggacttcg caagaaggtg agggtgttcc agcaagaaaa ctatctagcc aactttattc aaagcacttt taatgccatt gggaaacagg ggatgattcc cgataccctt gtccttggtg gtgatgggcg gtactttctc tctgaggcta ttcagatcat tatcaaattg gctgccgcca atggggtatc aaatgtttgg gtgggtaaag atggtcttct ctcaacccct gccgtgtcaa acatcatccg tcaacgaagg gatggtgatg tcacggcgaa gggggccttt attctcacag caagtcataa tcccggtggc cctgaggaag attttggtat caagtacaat acagaaaatg gaggacctgc ggctgagaaa attacttctg ctatttacga agagaccttg aaaattgatc attttctgac ctgcccaaat attggcacag tgaacgtgtc agaaatgggt gaccatttat ttgagaggtt cagagtttcg gtaatccata gcacagagga ttacgtccag agcatgaaga aaatctttga ctttcaaagt atccgaaatc tgttaaatag aactgatttt acgattcggt tggatggctt aagtggtatt gggggccctt atatgaagga tatttttgtt tctgccttgg gcgttccaga aagtgctttg tgcggtgcta cgccattgcc ggactttggt aagcaacatc cggatccaaa cctcacatat gcgaaggagc ttgtgagaac catgggactt gattccaccg gcaggcctgt tgctgacttt gttggcgagg tgccgaactt tgctgcggca tttgatgggg atgcagatcg taatatgatt cttggcgagc gcttttttgt aacaccgtct gattctttgg caattctttc agcaaatgca aatgtcgtgc cattttttgc tcaacaaggt ggaattaagg cggtagcgag atctatgccg acgagtggag ctgttgaccg tgttgcagaa atgcatcacc ttaaaatatt tgaagttccc acaggttgga agttttttgg gaatctgatg gacagccggg agttatttgg aggcgaagat tacaatcctc tcatttgtgg agaggaaagc tttggtactg gtagcaacca tattcgtgag aaggatggcg tgtgggcagc attattttgg ctatcggtaa ttgcgagtaa aaatgtggat ccgtcaaagc ctcttgtggg tgtaaaagac attgttgaag accactggac gcgctacggg cggaactact actgtcgata cgactatgaa aatgtggcag aggattcagc caaggctgtg atggagactg tacagaggca gcgcccccag gacattccct ccttgcaggg aaaacgctgt gtcaaggttg acaactttga gtaccacgac cctgtagacg gcttggtctc caaaaatcag ggcattcgtg tgatatttga ggatggttct cgatttgtga ttcgtttaag cggaactgga tcgtcaggcg ccacaattcg actatacctg gagcattaca tggagcccaa tgccgtagca cggcatatcc gtgacggcac gctgcctact cctcaatctg cgctggccaa tctcatcgcc gttgcgttga atgtctcaca gatttcggag cttacggggc gtgacgcccc cacagtcatc acttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat