TrcrA.17369.d

ATP synthase beta chain, mitochondrial precursor,ATP synthase F1, beta subunit

CENTER ID: TrcrA.17369.d
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.17369.d.B1.GE35194 full length 1 518
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053509233.180
RefSeq: XP_817834.1
UniProt: Q4DTX7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLTRLRSAAL RGAAGTRAAS GMPTADHKGR VGYVSQVIGA VVDVHFAEGV PPVLTALDVV EKLGRDEPLT LEIVQHLDAH TGRCIAMQTT DLLKLKSKVV SSGGNISVPV GRETLGRIFN VLGDAIDQRG PVGEKQRMPI HAVAPKLADQ AAEDTILTTG IKVIDLILPY CKGGKIGLFG GAGVGKTVII MELINNVAKG HGGFSVFAGV GERTREGTDL YLEMMQSKVI DLKGDSKCVL VYGQMNEPPG ARARVAQSAL TMAEYFRDVE GQDVLLFIDN IFRFTQANSE VSALLGRIPA AVGYQPTLAE DLGQLQERIT STTKGSITSV QAVYVPADDI TDPAPATTFS HLDATTVLDR AVAESGIYPA VNPLECASRI MDPDVISVDH YNVAQDVVQM LTKYKELQDI IAVLGIDELS EEDKLIVDRA RKVTKFLSQP FQVAEVFTGM TGHYVQLEET IESFSGLLMG TYDQVPEMAF YMVGGITSVL EKGKKMAEEA AELEKLRRAR AAQAGQKE
NT Sequence
atgttgaccc gtcttcgcag tgcagctttg cgcggggccg ccggtacacg cgccgcctca gggatgccta ccgccgatca caagggccgc gtcggctacg tgtcgcaggt tatcggcgct gttgtggatg tacactttgc ggaaggcgtg ccgccggtgt taactgcttt ggatgtggtg gaaaaacttg gccgtgacga gcccttgaca cttgaaattg tgcagcactt ggacgcccac accggccgtt gcattgcgat gcagacgaca gatctgctta agctgaaatc gaaggtagtc tcgagtggcg ggaacatttc tgtgcctgtg ggtcgggaga cacttggcag aatcttcaat gtgctgggcg atgccattga ccagcgcggc cctgttggag agaagcaacg catgcctatt cacgctgtgg caccaaagtt ggcggatcag gcggcagagg acaccattct cacaacgggt atcaaggtga ttgatctgat tttgccctac tgcaagggtg ggaagattgg tctttttggt ggtgctggcg tgggcaagac ggttattatc atggaactta ttaacaacgt tgccaagggc catggtgggt tctccgtctt tgcgggtgtt ggtgaacgca cgcgtgaggg aacggatctt tacctggaga tgatgcaatc caaagttatt gacctgaagg gtgactcgaa gtgtgtgttg gtgtatggtc agatgaacga gcctccgggt gcccgtgctc gtgtggcgca gtctgccttg acaatggccg agtacttccg tgatgtggaa gggcaagacg tgttgctttt tatcgacaac attttccgct ttacgcaggc aaactctgag gtgtcagcgc tgttgggtcg tattcccgct gccgtcggct accagcctac ccttgctgag gatcttggac agttgcagga gcgcattacg tccacgacaa aaggttccat tacctctgtg caggctgtgt acgtgccagc cgatgatatt accgaccctg cgccagcaac gaccttttcc cacctcgatg ccacgacggt gctggaccgt gccgttgccg aatccggcat ttaccccgct gtcaacccac tggagtgtgc ctcgcgtatc atggacccgg atgtgatcag cgttgaccac tacaacgtgg cgcaggatgt ggtgcagatg cttaccaagt acaaggagct gcaggatatt attgcggtgc ttggcattga tgagctcagt gaggaggata aacttatcgt ggaccgtgcg cgtaaggtga caaagtttct ctcccagcct ttccaggtgg cggaggtgtt tactggcatg acaggccact acgtgcagct ggaggagacc attgagtcct tctccggcct gttgatgggc acatatgatc aggtgccgga gatggcgttc tacatggtgg gcggcatcac gtccgtgctg gagaagggga agaagatggc agaggaggcc gcagaacttg aaaaacttcg tcgtgcccgt gccgcccagg caggccagaa ggaatag
Details for TrcrA.17369.d.B1.GE35194
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 71
Validated AA Sequence
MAHHHHHHML TRLRSAALRG AAGTRAASGM PTADHKGRVG YVSQVIGAVV DVHFAEGVPP VLTALDVVEK LGRDEPLTLE IVQHLDAHTG RCIAMQTTDL LKLKSKVVSS GGNISVPVGR ETLGRIFNVL GDAIDQRGPV GEKQRMPIHA VAPKLADQAA EDTILTTGIK VIDLILPYCK GGKIGLFGGA GVGKTVIIME LINNVAKGHG GFSVFAGVGE RTREGTDLYL EMMQSKVIDL KGDSKCVLVY GQMNEPPGAR ARVAXSALTM AXYFRDVEGQ DVLLFIDNIF RFTXXNSEXS ALLGRIPAAV GYQPTXAEDX XXXXXRITSR QXXXLXLXXX XXXXXXXXXX XXXPXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
ttgtttantt taagaaggag anataccatg gctcaccacc accaccacca tatgttgacc cgtcttcgca gtgcagcttt gcgcggggcc gccggtacac gcgccgcctc agggatgcct accgctgatc acaagggccg cgtcggttac gtgtcgcagg ttatcggcgc tgttgtggat gtacactttg cggaaggcgt gccgccggtg ttaactgctt tggatgtggt ggaaaaactt ggccgtgacg agcccttgac acttgaaatt gtgcagcact tggacgccca caccggccgt tgcattgcga tgcagacgac agatctgctt aagctgaaat cgaaggtagt ctcgagtggc gggaacattt ctgtgcctgt gggtcgggag acacttggca gaatcttcaa tgtgctgggc gatgccattg accagcgcgg ccctgttgga gagaagcaac gcatgcctat tcacgctgtg gcaccaaagt tggcggatca ggcggcagag gacaccattc ttacaacggg tatcaaggtg attgatctga ttttgcccta ctgcaagggt gggaagattg gtctttttgg tggtgctggc gtgggcaaga cggttattat catggaactt attaacaacg ttgccaaggg ccatggtggg ttctccgtct ttgcgggtgt tggtgaacgc acgcgtgagg gaacggatct ttacctggag atgatgcaat ccaaagttat tgacctgaag ggtgactcga agtgtgtgtt ggtgtatggt cagatgaacg agcctccggg tgcccgtgct cgtgtggcgc antctgcctt gacaatggcc gantacttcc gtgatgtgga agggcaagac gtgttgcttt ttatcgacaa cattttccgc tttacgcngg naaactctga gnngtcagcg ctgttgggtc gtattcccgc tgccgtcggc taccagccta ccnntgctga ggatcnnngn nagtngcngn agcgcattac gtcncgacaa aannntncat tanntctnnn cnnntnnnna cnnnnnnnna nnanantncn nncnncnncg annttnccan nnnnnnnnnn nnannnnnnt ncnnannnnn nttncncnnc nnnnnnnnnc nnnngnnnnn nnngnnnnnn na
Expected Protein Sequence
MAHHHHHHML TRLRSAALRG AAGTRAASGM PTADHKGRVG YVSQVIGAVV DVHFAEGVPP VLTALDVVEK LGRDEPLTLE IVQHLDAHTG RCIAMQTTDL LKLKSKVVSS GGNISVPVGR ETLGRIFNVL GDAIDQRGPV GEKQRMPIHA VAPKLADQAA EDTILTTGIK VIDLILPYCK GGKIGLFGGA GVGKTVIIME LINNVAKGHG GFSVFAGVGE RTREGTDLYL EMMQSKVIDL KGDSKCVLVY GQMNEPPGAR ARVAQSALTM AEYFRDVEGQ DVLLFIDNIF RFTQANSEVS ALLGRIPAAV GYQPTLAEDL GQLQERITST TKGSITSVQA VYVPADDITD PAPATTFSHL DATTVLDRAV AESGIYPAVN PLECASRIMD PDVISVDHYN VAQDVVQMLT KYKELQDIIA VLGIDELSEE DKLIVDRARK VTKFLSQPFQ VAEVFTGMTG HYVQLEETIE SFSGLLMGTY DQVPEMAFYM VGGITSVLEK GKKMAEEAAE LEKLRRARAA QAGQKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga cccgtcttcg cagtgcagct ttgcgcgggg ccgccggtac acgcgccgcc tcagggatgc ctaccgccga tcacaagggc cgcgtcggct acgtgtcgca ggttatcggc gctgttgtgg atgtacactt tgcggaaggc gtgccgccgg tgttaactgc tttggatgtg gtggaaaaac ttggccgtga cgagcccttg acacttgaaa ttgtgcagca cttggacgcc cacaccggcc gttgcattgc gatgcagacg acagatctgc ttaagctgaa atcgaaggta gtctcgagtg gcgggaacat ttctgtgcct gtgggtcggg agacacttgg cagaatcttc aatgtgctgg gcgatgccat tgaccagcgc ggccctgttg gagagaagca acgcatgcct attcacgctg tggcaccaaa gttggcggat caggcggcag aggacaccat tctcacaacg ggtatcaagg tgattgatct gattttgccc tactgcaagg gtgggaagat tggtcttttt ggtggtgctg gcgtgggcaa gacggttatt atcatggaac ttattaacaa cgttgccaag ggccatggtg ggttctccgt ctttgcgggt gttggtgaac gcacgcgtga gggaacggat ctttacctgg agatgatgca atccaaagtt attgacctga agggtgactc gaagtgtgtg ttggtgtatg gtcagatgaa cgagcctccg ggtgcccgtg ctcgtgtggc gcagtctgcc ttgacaatgg ccgagtactt ccgtgatgtg gaagggcaag acgtgttgct ttttatcgac aacattttcc gctttacgca ggcaaactct gaggtgtcag cgctgttggg tcgtattccc gctgccgtcg gctaccagcc tacccttgct gaggatcttg gacagttgca ggagcgcatt acgtccacga caaaaggttc cattacctct gtgcaggctg tgtacgtgcc agccgatgat attaccgacc ctgcgccagc aacgaccttt tcccacctcg atgccacgac ggtgctggac cgtgccgttg ccgaatccgg catttacccc gctgtcaacc cactggagtg tgcctcgcgt atcatggacc cggatgtgat cagcgttgac cactacaacg tggcgcagga tgtggtgcag atgcttacca agtacaagga gctgcaggat attattgcgg tgcttggcat tgatgagctc agtgaggagg ataaacttat cgtggaccgt gcgcgtaagg tgacaaagtt tctctcccag cctttccagg tggcggaggt gtttactggc atgacaggcc actacgtgca gctggaggag accattgagt ccttctccgg cctgttgatg ggcacatatg atcaggtgcc ggagatggcg ttctacatgg tgggcggcat cacgtccgtg ctggagaagg ggaagaagat ggcagaggag gccgcagaac ttgaaaaact tcgtcgtgcc cgtgccgccc aggcaggcca gaaggaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at