SSGCID: Target Page

TrcrA.17369.b

vacuolar ATP synthase subunit B, putative

CENTER ID:	TrcrA.17369.b
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.17369.b.B1.GE35192	full length		1	496

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053506025.50
RefSeq:	XP_808325.1
UniProt:	Q4D1S0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGIHEAEEVR MLSKQELLAE HIKAIQSGYS VKPHLEYTTI RAVNGPLVIL GDVRLPTFAE IVNIELADGT LRRGQVLEVD GDKAVVQVFE GTSGIDVMRS KCEFTGKVME LGVSEDMLGR VFNGSGIPID NGPPVLPEQY RDIQGIPINP RARVYPEEMI QTGISSIDVM TSISRGQKIP LFSGAGLPHN EIAAQIVRQA GLVRKEGKQE DFCIVFAAMG VNQETARFFR TEFEENGSME KTVLFMNLAN DPTIERIVTP RLALTTAEYL AYDCGKHVLV ILTDMSSYAD ALREVSAARE EVPGRRGFPG YMYTDLACIY ERAGRVLGRA GSITQIPILT MPNDDITHPI PDLTGYITEG QIYVDRQLHN RQLYPPINIL PSLSRLMKNA IGEGMTRKDH GGVSNQMYAA YAISRDILAM KAVVGEEALS SEDLLHLEFL EKFEKKFICQ GFYESRDVFQ SLDLCWQLLR TFPKVLLNKI DVKTRNEFYD RQPERK

NT Sequence

atgggcatac atgaggcaga ggaggttcgt atgctctcca agcaggagtt gttggcggag cacattaagg cgatccaaag cggttactct gtcaagccac accttgagta cacaacgatt cgcgccgtga atggaccgct ggtcatattg ggtgatgtgc gtctaccgac gtttgcggag attgtgaata tcgaattagc cgatggtacc cttcgccgcg ggcaggtgtt ggaggtggac ggagacaagg ccgttgtcca ggtgttcgag ggaacatccg gcatcgatgt catgcgctcc aaatgtgagt ttaccggaaa agtaatggaa ctcggcgtca gcgaagacat gttaggtcgt gtctttaacg gttctggcat tcctattgac aatggccccc ccgtattgcc tgagcagtac cgcgacattc aggggattcc aattaaccca cgggcacgtg tgtacccgga ggagatgatt cagaccggga tatcgtctat agacgtcatg acgtccattt cacgaggaca gaagattcca ctcttctctg gtgctggtct tcctcataat gaaattgctg cccagattgt gcgtcaggcc ggtttggttc gaaaggaagg aaaacaagag gatttctgca ttgtgtttgc agccatgggt gtgaaccagg agacagcacg attttttcgt actgagtttg aggaaaatgg atctatggaa aaaacagttc tgtttatgaa cttggccaat gatcccacaa ttgagcgcat cgtcacaccc cgtcttgctc ttaccaccgc cgagtacctt gcgtatgact gcggcaaaca tgtgttggtt attctgacag acatgagttc ctatgccgat gcccttcgcg aggtatcggc ggcgcgtgag gaggtccccg ggagacgcgg ttttcctgga tacatgtaca cggatttagc ctgcatctac gagcgtgctg gccgtgtttt gggacgtgca gggtccatca cgcagatccc tattcttacg atgcccaatg atgacatcac gcatcccatt ccggacctta cgggttacat cacagaaggt cagatttacg tggatcggca acttcataat cgtcagttgt acccgcccat caacatttta ccatccttaa gccgacttat gaagaatgcc attggtgagg gcatgacccg caaggaccat ggtggtgtca gcaaccaaat gtatgctgcg tacgccatca gtcgggatat tttggccatg aaggcggtcg ttggtgagga ggcactcagc agcgaggacc tccttcacct ggagtttttg gagaagtttg agaagaagtt tatttgtcag ggattctacg agtcccgcga tgtgtttcag agccttgatc tctgctggca gcttcttcgc accttcccga aggttttgct caacaagatt gacgtcaaga cgcgcaacga gttctacgac cgccaacccg agcggaagta g

Details for TrcrA.17369.b.B1.GE35192

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG IHEAEEVRML SKQELLAEHI KAIQSGYSVK PHLEYTTIRA VNGPLVILGD VRLPTFAEIV NIELADGTLR RGQVLEVDGD KAVVQVFEGT SGIDVMRSKC EFTGKVMELG VSEDMLGRVF NGSGIPIDNG PPVLPEQYRD IQGIPINPRA RVYPEEMIQT GISSIDVMTS ISRGQKIPLF SGAGLPHNEI AAQIVRQAGL VRKEGKQEDF CIVFAAMGVN QETARFFRTE FEENGSMEKT VLFMNLANDP TIERIVTPRL ALTTAEYLAY DCGKHVLVIL TDMSSYADAL REVSAAREEV PGRRGFPGYM YTDLACIYER AGRVLGRAGS ITQIPILTMP NDDITHPIPD LTGYITEGQI YVDRQLHNRQ LYPPINILPS LSRLMKNAIG EGMTRKDHGG VSNQMYAAYA ISRDILAMKA VVGEEALSSE DLLHLEFLEK FEKKFICQGF YESRDVFQSL DLCWQLLRTF PKVLLNKIDV KTRNEFYDRQ PERK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggca tacatgaggc agaggaggtt cgtatgctct ccaagcagga gttgttggcg gagcacatta aggcgatcca aagcggttac tctgtcaagc cacaccttga gtacacaacg attcgcgccg tgaatggacc gctggtcata ttgggtgatg tgcgtctacc gacgtttgcg gagattgtga atatcgaatt agccgatggt acccttcgcc gcgggcaggt gttggaggtg gacggagaca aggccgttgt ccaggtgttc gagggaacat ccggcatcga tgtcatgcgc tccaaatgtg agtttaccgg aaaagtaatg gaactcggcg tcagcgaaga catgttaggt cgtgtcttta acggttctgg cattcctatt gacaatggcc cccccgtatt gcctgagcag taccgcgaca ttcaggggat tccaattaac ccacgggcac gtgtgtaccc ggaggagatg attcagaccg ggatatcgtc tatagacgtc atgacgtcca tttcacgagg acagaagatt ccactcttct ctggtgctgg tcttcctcat aatgaaattg ctgcccagat tgtgcgtcag gccggtttgg ttcgaaagga aggaaaacaa gaggatttct gcattgtgtt tgcagccatg ggtgtgaacc aggagacagc acgatttttt cgtactgagt ttgaggaaaa tggatctatg gaaaaaacag ttctgtttat gaacttggcc aatgatccca caattgagcg catcgtcaca ccccgtcttg ctcttaccac cgccgagtac cttgcgtatg actgcggcaa acatgtgttg gttattctga cagacatgag ttcctatgcc gatgcccttc gcgaggtatc ggcggcgcgt gaggaggtcc ccgggagacg cggttttcct ggatacatgt acacggattt agcctgcatc tacgagcgtg ctggccgtgt tttgggacgt gcagggtcca tcacgcagat ccctattctt acgatgccca atgatgacat cacgcatccc attccggacc ttacgggtta catcacagaa ggtcagattt acgtggatcg gcaacttcat aatcgtcagt tgtacccgcc catcaacatt ttaccatcct taagccgact tatgaagaat gccattggtg agggcatgac ccgcaaggac catggtggtg tcagcaacca aatgtatgct gcgtacgcca tcagtcggga tattttggcc atgaaggcgg tcgttggtga ggaggcactc agcagcgagg acctccttca cctggagttt ttggagaagt ttgagaagaa gtttatttgt cagggattct acgagtcccg cgatgtgttt cagagccttg atctctgctg gcagcttctt cgcaccttcc cgaaggtttt gctcaacaag attgacgtca agacgcgcaa cgagttctac gaccgccaac ccgagcggaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat