SSGCID: Target Page

TrcrA.17369.a

V-type ATPase, A subunit, putative

CENTER ID:	TrcrA.17369.a
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.17369.a.B1.GE35191	full length		1	610

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053506405.120
RefSeq:	XP_812746.1
UniProt:	Q4DED4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MARDHNPHEA EHRMGSVKAV SGPVVIAENM SGSAMYELVQ VGSFRLVGEI IRLEGDTATI QVYEETGGLT VGDPVYCTGK PLSLELGPGI MSEIFDGIQR PLETIYRMVE NVFIPKGVQV KALNDQKMWD FKPRVRVGDL VTGGDILGTV VENSLMYDHS IMVPPNFQGR VTSVQPSGNY TLVDEIVEVE HNGTKRSLKL MHRWPVRTPR PVAVKESGNH PLLTGQRVLD ALFPSVQGGT CAIPGAFGCG KTVISQALSK YSNSDAVIYV GCGERGNEMA EVLMDFPTLT TVVDGREESI MKRTCLVANT SNMPVAAREA SIYTGITLAE YYRDMGKHIA MMADSTSRWA EALREISGRL AEMPADGGYP AYLSARLASF YERAGRVTCI GGPRREGSVT IVGAVSPPGG DFSDPVTSAT LGIVQVFWGL EKRLAQRKHF PSVNWLISYS KYLKVLEPFF NTFDPDYMRL RSVVAEILQR EEELQEIVQL VGKDSLSESD KIILETAKVI REEFLQQNAF TPYDKFCPLY KTCWMLRNIV TFYEEGQRVV AESAGEHKIT WNYIREKLPH IYNGLTEMKF RDPAEGKEAN IDYFKKQNEE IINAFASLLQ

NT Sequence

atggcgcgtg accacaaccc acatgaggcg gagcatcgca tgggatccgt aaaagccgtc tctggtccag tcgttattgc ggaaaacatg agcggtagcg caatgtacga gttggttcag gtcggttcct ttcgactggt gggagaaatc attcggcttg agggcgacac cgcgaccata caagtgtacg aggagacagg tggtcttaca gtcggggatc cggtttattg tacaggaaaa ccgctttctc ttgagcttgg tcctggcatt atgtcggaga tttttgatgg tattcaacgt ccacttgaga caatttaccg tatggtagaa aatgtattta ttccaaaggg agttcaggtt aaggcactca acgaccaaaa aatgtgggac tttaagcctc gtgtgagggt tggggatttg gtgacgggtg gggatattct tggaactgtt gtggaaaatt cgctcatgta cgatcactcc attatggtgc ctcccaattt tcaagggagg gttacgtccg ttcaaccttc tggtaactac acactcgtgg atgaaattgt ggaggtggag cacaatggta caaagagaag ccttaaacta atgcaccgtt ggcctgtgcg aacaccacgc ccggtggcgg tgaaggagtc cgggaatcat ccccttctta ctggtcaacg tgtcttggat gctctttttc cctctgtgca gggtggcaca tgcgcaattc ctggtgcatt tggttgtggt aagactgtta ttagtcaagc cctctccaaa tattcaaaca gcgatgctgt catttacgtg ggttgcggtg aacgtggtaa tgaaatggca gaggttttaa tggattttcc tactcttacc actgttgttg atggccgtga agaatctatc atgaaaagga catgcctcgt agccaatact tctaacatgc ctgtggctgc tcgtgaggcc tctatttaca ctggtattac cctcgccgaa tattatcgtg atatgggaaa acatattgcc atgatggcgg actccacttc ccgttgggca gaggctcttc gagagatttc tggtcgtttg gccgagatgc ctgctgatgg gggttatcct gcatatctca gtgcccgttt ggcttctttt tacgagcgtg caggtcgtgt cacatgcatt ggtggtccca gacgtgaagg atccgtcact attgtgggtg ctgtctcccc accaggaggt gatttttcgg atccggtaac atccgccaca cttggtatcg tgcaggtatt ctggggcctg gaaaaacgcc ttgcgcagcg taaacacttc ccctcagtaa actggcttat ttcgtactcg aagtacttaa aggtgctgga accctttttt aatacctttg accctgatta catgcgtttg cgctcggttg tcgctgagat tttgcaacgt gaagaggaat tgcaggaaat cgttcaactt gttggcaaag actctctttc agagtcggac aaaattatcc tggaaacggc gaaggtcatt cgtgaggagt tcctccagca aaacgccttt accccttacg acaagttttg cccactttat aaaacgtgtt ggatgctgcg caacattgtc accttctacg aggagggcca gcgtgttgtg gccgagtctg ctggcgaaca caaaatcacg tggaactaca tccgtgagaa gctaccgcat atttacaatg gactcacgga gatgaagttc cgcgaccccg cagaagggaa ggaggccaat attgactact ttaagaagca aaatgaggaa attattaatg catttgcctc ccttttgcag tag

Details for TrcrA.17369.a.B1.GE35191

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	68kDa
OBSERVED MW:	68kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	49

Validated AA Sequence

Validated NT Sequence

ttnnnnnntt taagaaggag atataccatg gctcaccacc accaccacca tatggcgcgt gaccacaacc cacatgaggc ggagcatcgc atgggatccg taaaagccgt ctctggtcca gtcgttattg cggaaaacat gagcggtagc gcaatgtacg agttggttca ggtcggttcc tttcgactgg tgggagaaat cattcggctt gagggcgaca ccgcgaccat acaagtgtac gaggagacag gtggtcttac agtcggggat ccggtttatt gtacaggaaa accgctttct cttgagcttg gtcctggcat tatgtcggag atttttgatg gtattcaacg tccacttgag acaatttacc gtatggtaga aaatgtattt attccaaagg gagttcaggt taaggcactc aacgaccaaa aaatgtggga ctttaagcct cgtgtgaggg ttggggattt ggtgacgggt ggggatattc ttggaactgt tgtggaaaat tcgctcatgt acgatcactc cattatggtg cctcccaatt ttcaagggag ggttacgtcc gttcaacctt ctggtaacta cacactcgtg gatgaaattg tggaggtgga gcacaatggt acaaagagaa gccttaaact aatgcaccgt tggcctgtgc gaacaccacg cccggtggcg gtgaaggagt ccgggaatca tccccttctt actggtcaac gtgtcttgga tgctcttttt ccctctgtgc agggtggcac atgcgcaatt cctggtgcat ttggttgtgg taagactgtt attagtcaag ccctctccaa atattcaaac agcgatgctg tcatttacgt gggttgcggt gaacgtggta atgaaatggc agagntttta atggattttn ctactcntan nactgttgtt gangnnngtn anaatctatc atgaaaanna catgnntcgt annnnactnc nanntnnngn ngntgctnnt gagnncnant tnnnctgnnt nccntcnnna anatnnnnng nnnnnnnaan nnnantnnnt nnngnnnnnt nnnnnnccng nnnngnnntn nnnnnnnnnn nnnnnnnnnn ncnannnnnn nnnnnnggnn nnnnnnnnnn nnnnnnnnnn nnnnnnntnn nnngncannn nnt

Expected Protein Sequence

MAHHHHHHMA RDHNPHEAEH RMGSVKAVSG PVVIAENMSG SAMYELVQVG SFRLVGEIIR LEGDTATIQV YEETGGLTVG DPVYCTGKPL SLELGPGIMS EIFDGIQRPL ETIYRMVENV FIPKGVQVKA LNDQKMWDFK PRVRVGDLVT GGDILGTVVE NSLMYDHSIM VPPNFQGRVT SVQPSGNYTL VDEIVEVEHN GTKRSLKLMH RWPVRTPRPV AVKESGNHPL LTGQRVLDAL FPSVQGGTCA IPGAFGCGKT VISQALSKYS NSDAVIYVGC GERGNEMAEV LMDFPTLTTV VDGREESIMK RTCLVANTSN MPVAAREASI YTGITLAEYY RDMGKHIAMM ADSTSRWAEA LREISGRLAE MPADGGYPAY LSARLASFYE RAGRVTCIGG PRREGSVTIV GAVSPPGGDF SDPVTSATLG IVQVFWGLEK RLAQRKHFPS VNWLISYSKY LKVLEPFFNT FDPDYMRLRS VVAEILQREE ELQEIVQLVG KDSLSESDKI ILETAKVIRE EFLQQNAFTP YDKFCPLYKT CWMLRNIVTF YEEGQRVVAE SAGEHKITWN YIREKLPHIY NGLTEMKFRD PAEGKEANID YFKKQNEEII NAFASLLQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc gtgaccacaa cccacatgag gcggagcatc gcatgggatc cgtaaaagcc gtctctggtc cagtcgttat tgcggaaaac atgagcggta gcgcaatgta cgagttggtt caggtcggtt cctttcgact ggtgggagaa atcattcggc ttgagggcga caccgcgacc atacaagtgt acgaggagac aggtggtctt acagtcgggg atccggttta ttgtacagga aaaccgcttt ctcttgagct tggtcctggc attatgtcgg agatttttga tggtattcaa cgtccacttg agacaattta ccgtatggta gaaaatgtat ttattccaaa gggagttcag gttaaggcac tcaacgacca aaaaatgtgg gactttaagc ctcgtgtgag ggttggggat ttggtgacgg gtggggatat tcttggaact gttgtggaaa attcgctcat gtacgatcac tccattatgg tgcctcccaa ttttcaaggg agggttacgt ccgttcaacc ttctggtaac tacacactcg tggatgaaat tgtggaggtg gagcacaatg gtacaaagag aagccttaaa ctaatgcacc gttggcctgt gcgaacacca cgcccggtgg cggtgaagga gtccgggaat catccccttc ttactggtca acgtgtcttg gatgctcttt ttccctctgt gcagggtggc acatgcgcaa ttcctggtgc atttggttgt ggtaagactg ttattagtca agccctctcc aaatattcaa acagcgatgc tgtcatttac gtgggttgcg gtgaacgtgg taatgaaatg gcagaggttt taatggattt tcctactctt accactgttg ttgatggccg tgaagaatct atcatgaaaa ggacatgcct cgtagccaat acttctaaca tgcctgtggc tgctcgtgag gcctctattt acactggtat taccctcgcc gaatattatc gtgatatggg aaaacatatt gccatgatgg cggactccac ttcccgttgg gcagaggctc ttcgagagat ttctggtcgt ttggccgaga tgcctgctga tgggggttat cctgcatatc tcagtgcccg tttggcttct ttttacgagc gtgcaggtcg tgtcacatgc attggtggtc ccagacgtga aggatccgtc actattgtgg gtgctgtctc cccaccagga ggtgattttt cggatccggt aacatccgcc acacttggta tcgtgcaggt attctggggc ctggaaaaac gccttgcgca gcgtaaacac ttcccctcag taaactggct tatttcgtac tcgaagtact taaaggtgct ggaacccttt tttaatacct ttgaccctga ttacatgcgt ttgcgctcgg ttgtcgctga gattttgcaa cgtgaagagg aattgcagga aatcgttcaa cttgttggca aagactctct ttcagagtcg gacaaaatta tcctggaaac ggcgaaggtc attcgtgagg agttcctcca gcaaaacgcc tttacccctt acgacaagtt ttgcccactt tataaaacgt gttggatgct gcgcaacatt gtcaccttct acgaggaggg ccagcgtgtt gtggccgagt ctgctggcga acacaaaatc acgtggaact acatccgtga gaagctaccg catatttaca atggactcac ggagatgaag ttccgcgacc ccgcagaagg gaaggaggcc aatattgact actttaagaa gcaaaatgag gaaattatta atgcatttgc ctcccttttg cagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat