SSGCID: Target Page

TrcrA.17275.a

2,3-bisphosphoglycerate-independent phosphoglycerate mutase

CENTER ID:	TrcrA.17275.a
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.17275.a.B1.GE35190	full length		1	554

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.17275.a.B1.PS01517	full length		1	554

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053506247.330
OrthoMCL:	OG5_129051
RefSeq:	XP_820347.1
UniProt:	Q4E122

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSALSLQLHT ALPRRKLLLV VMDGVGIGPG DEYDAVHVAK TPFIDSMCAD AKHFRSVCAH GTAVGLPTDA DMGNSEVGHN ALGSGRVVLQ GASLVDDAIK TGEIFTSDGY RYLHGAFSQP GRTLHLIGLL SDGGVHSRDN QLYEIINHAS SNGAKRIRLH VLYDGRDVPD KSSFKFTDDL ESVLEKARAK GCDARIASGG GRMFVTMDRY EADWSVVERG WRAQVLGEAR AFASAGEAIK TFRKEDPNVS DQYYPPFIIS DVDGKAIGPI EDGDAVLCFN FRGDRVIEMS RAFEEEDFDK FNRVRVPKVR YAGMMRYDGD LGIPNNFLVP PPRLSRTSEE YLVGSGCNIF ACSETQKFGH VTYFWNGNRS GKLDEEHETF FEIPSDRVQF NEKPLMKSKE ITEAAIEALK SGKYDVVRIN FPNGDMVGHT GDLAATVTAV EAVDASLQRL KEAVDAVNGV FLITADHGNS DDMAQRDKKG KPILGKDGKV LPLTSHTLAP VPVFIGGAGL DARVQMRTGL PKAGLANVTA TFLNLMGFTA PTDYEPSLIE VIPN

NT Sequence

atgtcagcgc tttctcttca gcttcatacc gcactccccc gacgcaagtt gctgcttgtg gttatggacg gtgtcggtat tggtcctggc gatgagtacg acgcggtcca cgttgccaaa acgcccttta ttgactcgat gtgcgccgac gcaaagcact ttcgttccgt ctgcgctcat ggcactgcag tggggctccc cacagacgcg gatatgggga acagtgaggt ggggcacaac gcacttggtt caggccgtgt tgtgttgcag ggagcctccc ttgttgacga tgccatcaaa actggtgaga tttttaccag cgatggctac cgttaccttc acggcgcctt ctcgcagcca ggtcgcaccc ttcacttgat tggcttgcta agtgacggcg gtgttcactc ccgcgacaac cagctctatg agatcatcaa ccatgccagt tcgaatggag cgaagcgtat tcgtctgcat gtcctctatg atggccgtga tgtgcctgat aagagttcat tcaaattcac tgacgatctg gaatctgtcc ttgaaaaggc tcgtgcaaag ggttgtgatg cccgcattgc aagcggtggt ggtcgcatgt ttgtcacgat ggatcgttac gaggcagact ggtctgtagt cgagcgtggc tggagggcgc aagtgttggg tgaggcaagg gccttcgctt ctgctggtga ggccatcaaa acattccgca aagaggaccc aaatgtttcg gatcagtact atccaccttt catcatcagc gacgtggacg gcaaagccat tggccctatt gaggatggcg atgctgtgct ttgctttaac ttccgtggcg atcgtgtcat tgagatgtcc cgtgcctttg aggaagagga ttttgataaa ttcaaccgtg ttcgtgttcc caaggtgcgc tacgctggca tgatgcgata cgacggcgat ctgggtattc ctaataattt tttggtgccg ccacccaggc tttctcggac gagcgaggaa tatctcgttg gctctggatg caatatcttc gcctgctccg agacacaaaa atttggccac gtgacgtatt tctggaacgg caaccgcagt ggaaagttgg atgaggagca cgagacgttc tttgagattc ccagcgatcg tgtacagttc aatgaaaaac ccctcatgaa gagcaaagaa atcaccgagg cggcaattga ggcattgaag agtggcaagt acgatgtcgt gcgcatcaat ttccccaatg gtgatatggt gggacacacg ggtgacctcg cagcgacggt caccgctgtt gaggcagtgg acgcatcatt acagcggctg aaggaagccg ttgatgctgt taacggcgtt ttcctcatta ccgcagatca cggcaactcc gacgacatgg cgcagcgcga caagaagggg aagccaatcc ttggtaaaga cggaaaagta cttccgctca catcgcatac actcgcgcct gttcctgtgt tcattggtgg tgccgggctg gatgcacgtg tgcagatgcg cacgggcctc cctaaggccg ggctggcgaa tgtgacagcg acctttctga atctgatggg atttactgct ccgacggact acgagccatc actcattgag gtgatcccga actga

Details for TrcrA.17275.a.B1.GE35190

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	61kDa
OBSERVED MW:	65kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Validated AA Sequence

MDGVGIGPGD EYDAVHVAKT PFIDSMCADA KHFRSVCAHG TAVGLPTDAD MGNSEVGHNA LGSGRVVLQG ASLVDDAIKT GEIFTSDGYR YLHGAFSQPG RTLHLIGLLS DGGVHSRDNQ LYEIINHASS NGAKRIRLHV LYDGRDVPDK SSFKFTDDLE SVLEKARAKG CDARIASGGG RMFVTMDRYE ADWSVVERGW KAQVLGEARA FASASEAIKT FRKEDPNVSD QYYPPFIISD VDGKAIGPIE DGDAVLCFNF RGDRVIEMSR AFEEEDFDKF NRVRVPKVRY AGMMRYDGDL GIPNNFXVPP PRLSRTSEEY LVGSGCNIFA CSETQKFGHV TYFWNGNRSG KLDEEHETFF EIPSDRVQFN EKPLMKSKEI TEAAIEALKS GNYDVVRINF PNGDMVGHTG DLAATVTAVE AVDASLQRLK EAVDAVNGVF LITADHGNSD DMAQRDKKGK PILGKDGKVL PLTSHTLAPV PVFIGGAGLD ARVQMRTGLP KAGLANVTAT FLNLMGFTAP TDYE

Validated NT Sequence

gctcgtagtc cgtcggagca gtaaatccca tcagattcag aaaggtcgct gtcacattcg ccagcccggc cttagggagg cccgtgcgca tctgcacacg tgcatccagc ccggcaccac caatgaacac aggaacaggc gcgagtgtat gcgatgtgag cggaagtact tttccgtctt taccaaggat tggcttcccc ttcttgtcgc gctgcgccat gtcgtcggag ttgccgtgat ctgcggtaat gaggaaaacg ccgttaacag catcaacggc ttccttcagc cgctgtaatg atgcgtccac tgcctcaaca gcggtgaccg tcgctgcgag gtcacccgtg tgtcccacca tatcaccatt ggggaaattg atgcgcacga catcgtaatt gccactcttc aatgcctcaa ttgccgcctc ggtgatttct ttgctcttca tgaggggttt ttcattgaac tgtacacgat cgctgggaat ctcaaagaac gtctcgtgct cctcatccaa ctttccactg cggttgccgt tccagaaata cgtcacgtgg ccaaattttt gtgtctcgga gcaggcgaag atattgcatc cagagccnac gagatattcc tcgctcgtcc gagaaagcct gggtggcggc accnaaaaat tattaggaat acccagatcg ccgtcgtanc gcatcatgcc agcgtagcgc accttgggaa cacgaacacg gttgaattta tcaaaatcct cttcctcaaa ggcacgggac atctcaatga cacgatcgcc acggaagttg aagcaaagca cagcatcgcc atcctcaata gggccaatgg ctttgccgtc cacgtcgctg atgatgaaag gtggatagta ctgatccgaa acatttgggt cctctttgcg gaatgttttg atggcctcac tagcagaagc gaaggccctt gcctcaccca acacttgcgc cttccagcca cgctcgacta cagaccagtc tgcctcgtaa cgatccatcg taacaaacat gcgaccacca ccgcttgcaa tgcgggcatc acaacccttt gcacgagcct tttcaaggac cgattccaga tcgtcagtga atttgaatga actcttatca ggcacatcac ggccatcata gaggacatgc agacgaatac gcttcgctcc attcgaactg gcatggttga tgatctcata gagctggttg tcgcgggagt gaacaccgcc gtcacttagc aagccaatca agtgaagggt gcgacctggc tgcgagaagg cgccgtgaag gtaacggtag ccatcgctgg taaaaatctc accagttttg atggcatcgt caacaaggga ggctccctgc aacacaacac ggcctgaacc aagtgcgttg tgccccacct cactgttccc catatccgcg tctgtgggga gccccactgc agtgccatga gcgcagacgg aacgaaagtg ctttgcgtcg gcgcacatcg agtcaataaa gggcgttttg gcaacgtgga ccgcgtcgta ctcatcgcca ggaccaatac cgacaccgtc cataaccaca agcagcaact tgcgtcgggg gagtgcggta tgaagctgaa gagaaagc

Expected Protein Sequence

MAHHHHHHMS ALSLQLHTAL PRRKLLLVVM DGVGIGPGDE YDAVHVAKTP FIDSMCADAK HFRSVCAHGT AVGLPTDADM GNSEVGHNAL GSGRVVLQGA SLVDDAIKTG EIFTSDGYRY LHGAFSQPGR TLHLIGLLSD GGVHSRDNQL YEIINHASSN GAKRIRLHVL YDGRDVPDKS SFKFTDDLES VLEKARAKGC DARIASGGGR MFVTMDRYEA DWSVVERGWR AQVLGEARAF ASAGEAIKTF RKEDPNVSDQ YYPPFIISDV DGKAIGPIED GDAVLCFNFR GDRVIEMSRA FEEEDFDKFN RVRVPKVRYA GMMRYDGDLG IPNNFLVPPP RLSRTSEEYL VGSGCNIFAC SETQKFGHVT YFWNGNRSGK LDEEHETFFE IPSDRVQFNE KPLMKSKEIT EAAIEALKSG KYDVVRINFP NGDMVGHTGD LAATVTAVEA VDASLQRLKE AVDAVNGVFL ITADHGNSDD MAQRDKKGKP ILGKDGKVLP LTSHTLAPVP VFIGGAGLDA RVQMRTGLPK AGLANVTATF LNLMGFTAPT DYEPSLIEVI PN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcag cgctttctct tcagcttcat accgcactcc cccgacgcaa gttgctgctt gtggttatgg acggtgtcgg tattggtcct ggcgatgagt acgacgcggt ccacgttgcc aaaacgccct ttattgactc gatgtgcgcc gacgcaaagc actttcgttc cgtctgcgct catggcactg cagtggggct ccccacagac gcggatatgg ggaacagtga ggtggggcac aacgcacttg gttcaggccg tgttgtgttg cagggagcct cccttgttga cgatgccatc aaaactggtg agatttttac cagcgatggc taccgttacc ttcacggcgc cttctcgcag ccaggtcgca cccttcactt gattggcttg ctaagtgacg gcggtgttca ctcccgcgac aaccagctct atgagatcat caaccatgcc agttcgaatg gagcgaagcg tattcgtctg catgtcctct atgatggccg tgatgtgcct gataagagtt cattcaaatt cactgacgat ctggaatctg tccttgaaaa ggctcgtgca aagggttgtg atgcccgcat tgcaagcggt ggtggtcgca tgtttgtcac gatggatcgt tacgaggcag actggtctgt agtcgagcgt ggctggaggg cgcaagtgtt gggtgaggca agggccttcg cttctgctgg tgaggccatc aaaacattcc gcaaagagga cccaaatgtt tcggatcagt actatccacc tttcatcatc agcgacgtgg acggcaaagc cattggccct attgaggatg gcgatgctgt gctttgcttt aacttccgtg gcgatcgtgt cattgagatg tcccgtgcct ttgaggaaga ggattttgat aaattcaacc gtgttcgtgt tcccaaggtg cgctacgctg gcatgatgcg atacgacggc gatctgggta ttcctaataa ttttttggtg ccgccaccca ggctttctcg gacgagcgag gaatatctcg ttggctctgg atgcaatatc ttcgcctgct ccgagacaca aaaatttggc cacgtgacgt atttctggaa cggcaaccgc agtggaaagt tggatgagga gcacgagacg ttctttgaga ttcccagcga tcgtgtacag ttcaatgaaa aacccctcat gaagagcaaa gaaatcaccg aggcggcaat tgaggcattg aagagtggca agtacgatgt cgtgcgcatc aatttcccca atggtgatat ggtgggacac acgggtgacc tcgcagcgac ggtcaccgct gttgaggcag tggacgcatc attacagcgg ctgaaggaag ccgttgatgc tgttaacggc gttttcctca ttaccgcaga tcacggcaac tccgacgaca tggcgcagcg cgacaagaag gggaagccaa tccttggtaa agacggaaaa gtacttccgc tcacatcgca tacactcgcg cctgttcctg tgttcattgg tggtgccggg ctggatgcac gtgtgcagat gcgcacgggc ctccctaagg ccgggctggc gaatgtgaca gcgacctttc tgaatctgat gggatttact gctccgacgg actacgagcc atcactcatt gaggtgatcc cgaactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for TrcrA.17275.a.B1.PS01517

PURIFICATION DATe:	5/8/2012
CONCENTRATION:	56.6mg/ml
OBSERVED MW:	64kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAG CGCTTTCTCT TCAGCTTCAT ACCGCACTCC CCCGACGCAA GTTGCTGCTT GTGGTTATGG ACGGTGTCGG TATTGGTCCT GGCGATGAGT ACGACGCGGT CCACGTTGCC AAAACGCCCT TTATTGACTC GATGTGCGCC GACGCAAAGC ACTTTCGTTC CGTCTGCGCT CATGGCACTG CAGTGGGGCT CCCCACAGAC GCGGATATGG GGAACAGTGA GGTGGGGCAC AACGCACTTG GTTCAGGCCG TGTTGTGTTG CAGGGAGCCT CCCTTGTTGA CGATGCCATC AAAACTGGTG AGATTTTTAC CAGCGATGGC TACCGTTACC TTCACGGCGC CTTCTCGCAG CCAGGTCGCA CCCTTCACTT GATTGGCTTG CTAAGTGACG GCGGTGTTCA CTCCCGCGAC AACCAGCTCT ATGAGATCAT CAACCATGCC AGTTCGAATG GAGCGAAGCG TATTCGTCTG CATGTCCTCT ATGATGGCCG TGATGTGCCT GATAAGAGTT CATTCAAATT CACTGACGAT CTGGAATCTG TCCTTGAAAA GGCTCGTGCA AAGGGTTGTG ATGCCCGCAT TGCAAGCGGT GGTGGTCGCA TGTTTGTCAC GATGGATCGT TACGAGGCAG ACTGGTCTGT AGTCGAGCGT GGCTGGAGGG CGCAAGTGTT GGGTGAGGCA AGGGCCTTCG CTTCTGCTGG TGAGGCCATC AAAACATTCC GCAAAGAGGA CCCAAATGTT TCGGATCAGT ACTATCCACC TTTCATCATC AGCGACGTGG ACGGCAAAGC CATTGGCCCT ATTGAGGATG GCGATGCTGT GCTTTGCTTT AACTTCCGTG GCGATCGTGT CATTGAGATG TCCCGTGCCT TTGAGGAAGA GGATTTTGAT AAATTCAACC GTGTTCGTGT TCCCAAGGTG CGCTACGCTG GCATGATGCG ATACGACGGC GATCTGGGTA TTCCTAATAA TTTTTTGGTG CCGCCACCCA GGCTTTCTCG GACGAGCGAG GAATATCTCG TTGGCTCTGG ATGCAATATC TTCGCCTGCT CCGAGACACA AAAATTTGGC CACGTGACGT ATTTCTGGAA CGGCAACCGC AGTGGAAAGT TGGATGAGGA GCACGAGACG TTCTTTGAGA TTCCCAGCGA TCGTGTACAG TTCAATGAAA AACCCCTCAT GAAGAGCAAA GAAATCACCG AGGCGGCAAT TGAGGCATTG AAGAGTGGCA AGTACGATGT CGTGCGCATC AATTTCCCCA ATGGTGATAT GGTGGGACAC ACGGGTGACC TCGCAGCGAC GGTCACCGCT GTTGAGGCAG TGGACGCATC ATTACAGCGG CTGAAGGAAG CCGTTGATGC TGTTAACGGC GTTTTCCTCA TTACCGCAGA TCACGGCAAC TCCGACGACA TGGCGCAGCG CGACAAGAAG GGGAAGCCAA TCCTTGGTAA AGACGGAAAA GTACTTCCGC TCACATCGCA TACACTCGCG CCTGTTCCTG TGTTCATTGG TGGTGCCGGG CTGGATGCAC GTGTGCAGAT GCGCACGGGC CTCCCTAAGG CCGGGCTGGC GAATGTGACA GCGACCTTTC TGAATCTGAT GGGATTTACT GCTCCGACGG ACTACGAGCC ATCACTCATT GAGGTGATCC CGAACTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT