TrcrA.17127.a
glucose-6-phosphate isomerase
| CENTER ID: | TrcrA.17127.a |
| ORGANISM: | Trypanosoma cruzi |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrcrA.17127.a.B1.GE35188 | full length | 1 | 608 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrcrA.17127.a.B1.PS01519 | full length | 1 | 608 |
Structures
4QFH
DEPOSITED: 5/21/2014
DETERMINATION: XRay
CLONE: TrcrA.17127.a.B1.GE35188
PROTEIN: TrcrA.17127.a.B1.PS01519
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:Tc00.1047053506529.508 |
| OrthoMCL: | OG5_126980 |
| RefSeq: | XP_821969.1 |
| UniProt: | O61113 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKDQYLKDLT VHLNESNAAP ANTSMAVASF NMPHEITRRM RPLGVDADTS LTSCPSWRRL QELYEIHGSE SILKNFDECK DRFQRYSLEV DLRSSDKNFV FLDYSKTHIN DEIKDVLFKL VEERGIRAFM RALFAGEKVN TAENRSVLHI ALRNRSNRPI FVNGHDVMPL VNKVLEQMKK LSEKVRRGEW KGQSGKPIRH VVNIGIGGSD LGPMMACEAL RPFSDRRISM HFVSNIDGTH LSEVLNLVDL ESTLFIIASK TFTTQETITN ALSARNEFLK FLSSRGISEA GAVAKHFVAL STNAEKVKEF GIDEENMFQF WDWVGGRYSL WSAIGLSVMI SIGYDNFVEL LTGAHIMDEH FINAPTENNL PIILALVGIW YNNFFGSETQ AILPYDQYLW RLPAYLQQLD MESNGKGATK NGRMVSTHTG PIIFGEAGTN GQHAFYQLIH QGTKLIPCDF IGAIQTQNYI GEHHRILMSN FFAQTEALMI GKTPEEVKRE LESAGGKSED EIQLLIPQKT FTGGRPSNSL LVKALTPRAL GAIIAMYEHK VLVQGAIWGI NSYDQWGVEL GKLLAKSILL QLQPGQKVTN HDSSTNGLIE LFNERSHL
NT Sequence
atgaaagatc aatacctgaa ggatttgaca gttcacttga acgaaagcaa tgcggcaccc gcgaatacca gcatggctgt cgcatctttc aatatgccac acgaaataac acgaaggatg aggccacttg gtgttgatgc tgacacaagc ttgacttcgt gcccctcctg gcgtcgtctt caagaattat acgagatcca cggaagcgag agcattttga agaatttcga cgaatgtaag gatcgtttcc aacgatactc gcttgaagtg gatttgagaa gcagtgacaa aaacttcgtt tttttggatt actcaaagac acacataaat gatgaaataa aagacgttct ttttaagttg gttgaagaga gaggtattcg agcgtttatg cgggcattgt ttgctggaga gaaggttaat acggccgaaa atcgttcagt gcttcatatt gctttgcgaa atcgaagcaa tcgtcccatt tttgtgaatg gacatgacgt tatgcccttg gtaaacaagg tacttgaaca aatgaagaaa ctatcagaaa aggtcaggag aggtgaatgg aaaggtcagt caggaaaacc tatccgccat gttgtgaata ttgggattgg aggcagtgat ctcggtccca tgatggcatg tgaagctttg aggccttttt cagatcggag aatatccatg cattttgttt ctaatattga tgggacacac ttgtctgaag tcttgaattt ggtcgacttg gaatcaactc tttttataat tgccagcaaa acattcacta cacaagaaac catcacgaac gctttgtccg cacggaatga gtttttgaag tttttaagtt ctcggggtat ttcagaagct ggtgctgttg caaagcactt tgttgcgctt tcaacaaatg cggaaaaagt caaagaattt ggtattgatg aagagaatat gttccaattt tgggactggg tcggtggccg atactcattg tggtccgcca ttggtttatc tgtcatgatt tcaattgggt acgacaattt tgtggagttg ttaactggtg cacacatcat ggatgaacac tttatcaatg cacccacgga aaacaatctc cctattattt tggctttggt tggtatttgg tacaataact tctttggaag tgaaacccaa gctatcctac catatgatca atacttatgg aggttgccag cgtaccttca acagcttgac atggaaagca atggaaaggg tgccaccaaa aacggccgaa tggtgtcaac gcacactggt cctattattt ttggggaagc tggcaccaac ggccaacatg ctttttatca acttattcat caagggacaa agttaattcc gtgcgacttc atcggcgcga ttcaaactca aaattatatt ggagaacatc accgaatttt gatgagcaac ttttttgctc agacagaggc actgatgatt ggtaagaccc ctgaggaggt taaaagggaa ctggaatccg cgggaggaaa gtctgaggat gagattcaat tgttgatacc acaaaaaacg ttcactgggg gacgtccaag caactccttg ttggtaaagg ctctcactcc acgggcattg ggtgctatta ttgccatgta tgagcacaaa gttcttgtcc aaggagctat ttggggaatt aacagctatg atcaatgggg tgtggaactg gggaaattac ttgccaagtc cattcttcta caacttcagc ctggccaaaa ggttacaaac catgattctt caacaaatgg tctcattgag ctattcaacg aacgctctca cttgtaa
Details for TrcrA.17127.a.B1.GE35188
| HARVESTED ON: | 4/24/2012 |
| SEQUENCED ON: | 8/7/2014 |
| EXPECTED MW: | 69kDa |
| OBSERVED MW: | 69kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 90 |
| PERCENT COVERAGE: | 59 |
Validated AA Sequence
MAHHHHHHMK DQYLKDLTVH LNESNAAPAN TSMAVASFNM PHEITRRMRP LGVDADTSLT SCPSWRRLQE LYEIHGSESI LKNFDECKDR FQRYSLEVDL RSSDKNFVFL DYSKTHINDE IKDVLFKLVE ERGIRAFMRA LFAGEKVNTA ENRSVLHIAL RNRSNRPIFV NGHDVMPLVN KVLEQMKKLS EKVRRGEWKG QSGKPIRHVV NIGIGGSDLG PMMACEALRP FSDRRISMHF VSNIDGTHLS EVLNLVDLES TLFIIASKTF TTQETITNAL SARNEFLKFL SSRGISEAGA VAKHFVXLST NAXKVKEFGX XXXNMXXFXX LGRWXILXXV XHXFXCHDFN WXRQFCGVVN WCTHHG
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa gatcaatacc tgaaggattt gacagttcac ttgaacgaaa gcaatgcggc acccgcgaat accagcatgg ctgtcgcatc tttcaatatg ccacacgaaa taacacgaag gatgaggcca cttggtgttg atgctgacac aagcttgact tcgtgcccct cctggcgtcg tcttcaagaa ttatacgaga tccacggaag cgagagcatt ttgaagaatt tcgacgaatg taaggatcgt ttccaacgat actcgcttga agtggatttg agaagcagtg acaaaaactt cgtttttttg gattactcaa agacacacat aaatgatgaa ataaaagacg ttctttttaa gttggttgaa gagagaggta ttcgagcgtt tatgcgggca ttgtttgctg gagagaaggt taatacggcc gaaaatcgtt cagtgcttca tattgctttg cgaaatcgaa gcaatcgtcc catttttgtg aatggacatg acgttatgcc cttggtaaac aaggtacttg aacaaatgaa gaaactatca gaaaaggtca ggagaggtga atggaaaggt cagtcaggaa aacctatccg ccatgttgtg aatattggga ttggaggcag tgatctcggt cccatgatgg catgtgaagc tttgaggcct ttttcagatc ggagaatatc catgcatttt gtttctaata ttgatgggac acacttgtct gaagtcttga atttggtcga cttggaatca actcttttta taattgccag caaaacattc actacacaag aaaccatcac gaacgctttg tccgcacgga atgagttttt gaagttttta agttctcggg gtatttcaga agctggtgct gttgcaaagc actttgttgn gctttcaaca aatgcgnaaa aagtcaaaga atttggtant gangangnna atatgtnnna nttttnggna ctgggtcggt ggnnnatact catngnggtc ngccatnggt ttntctgtca tgatttcaat tggntacgac aattttgtgg agttgttaac tggtgcacac atcatggatg ancacttnat caatgcaccc acggaaanca ntctccntat tattttggct ttggttggta tnnggtacaa taanttcttt ggaagtgaaa cccaagctat cctaccatat gatcaatact tatggaggtt gccagcgtac cttcaacagc ttgacatgga aagcaatgga aagggtgcca ccaaaaacgg ccgaatggtg tcaacgcaca ctggtcctat tatttttggg gaagctggca ccaacggcca acatgctttt tatcaactta ttcatcaagg gacaaagtta attccgtgcg acttcatcgg cgcgattcaa actcaaaatt atattggaga acatcaccga attttgatga gcaacttttt tgctcagaca gaggcactga tgattggtaa gacccctgag gaggttaaaa gggaactgga atccgcggga ggaaagtctg aggatgagat tcaattgttg ataccacaaa aaacgttcac tgggggacgt ccaagcaact ccttgttggt aaaggctctc actccacggg cattgggtgc tattattgcc atgtatgagc acaaagttct tgtccaagga gctatttggg gaattaacag ctatgatcaa tggggtgtgg aactggggaa attacttgcc aagtccattc ttctacaact tcagcctggc caaaaggtta caaaccatga ttcttcaaca aatggtctca ttgagc
Expected Protein Sequence
MAHHHHHHMK DQYLKDLTVH LNESNAAPAN TSMAVASFNM PHEITRRMRP LGVDADTSLT SCPSWRRLQE LYEIHGSESI LKNFDECKDR FQRYSLEVDL RSSDKNFVFL DYSKTHINDE IKDVLFKLVE ERGIRAFMRA LFAGEKVNTA ENRSVLHIAL RNRSNRPIFV NGHDVMPLVN KVLEQMKKLS EKVRRGEWKG QSGKPIRHVV NIGIGGSDLG PMMACEALRP FSDRRISMHF VSNIDGTHLS EVLNLVDLES TLFIIASKTF TTQETITNAL SARNEFLKFL SSRGISEAGA VAKHFVALST NAEKVKEFGI DEENMFQFWD WVGGRYSLWS AIGLSVMISI GYDNFVELLT GAHIMDEHFI NAPTENNLPI ILALVGIWYN NFFGSETQAI LPYDQYLWRL PAYLQQLDME SNGKGATKNG RMVSTHTGPI IFGEAGTNGQ HAFYQLIHQG TKLIPCDFIG AIQTQNYIGE HHRILMSNFF AQTEALMIGK TPEEVKRELE SAGGKSEDEI QLLIPQKTFT GGRPSNSLLV KALTPRALGA IIAMYEHKVL VQGAIWGINS YDQWGVELGK LLAKSILLQL QPGQKVTNHD SSTNGLIELF NERSHL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag atcaatacct gaaggatttg acagttcact tgaacgaaag caatgcggca cccgcgaata ccagcatggc tgtcgcatct ttcaatatgc cacacgaaat aacacgaagg atgaggccac ttggtgttga tgctgacaca agcttgactt cgtgcccctc ctggcgtcgt cttcaagaat tatacgagat ccacggaagc gagagcattt tgaagaattt cgacgaatgt aaggatcgtt tccaacgata ctcgcttgaa gtggatttga gaagcagtga caaaaacttc gtttttttgg attactcaaa gacacacata aatgatgaaa taaaagacgt tctttttaag ttggttgaag agagaggtat tcgagcgttt atgcgggcat tgtttgctgg agagaaggtt aatacggccg aaaatcgttc agtgcttcat attgctttgc gaaatcgaag caatcgtccc atttttgtga atggacatga cgttatgccc ttggtaaaca aggtacttga acaaatgaag aaactatcag aaaaggtcag gagaggtgaa tggaaaggtc agtcaggaaa acctatccgc catgttgtga atattgggat tggaggcagt gatctcggtc ccatgatggc atgtgaagct ttgaggcctt tttcagatcg gagaatatcc atgcattttg tttctaatat tgatgggaca cacttgtctg aagtcttgaa tttggtcgac ttggaatcaa ctctttttat aattgccagc aaaacattca ctacacaaga aaccatcacg aacgctttgt ccgcacggaa tgagtttttg aagtttttaa gttctcgggg tatttcagaa gctggtgctg ttgcaaagca ctttgttgcg ctttcaacaa atgcggaaaa agtcaaagaa tttggtattg atgaagagaa tatgttccaa ttttgggact gggtcggtgg ccgatactca ttgtggtccg ccattggttt atctgtcatg atttcaattg ggtacgacaa ttttgtggag ttgttaactg gtgcacacat catggatgaa cactttatca atgcacccac ggaaaacaat ctccctatta ttttggcttt ggttggtatt tggtacaata acttctttgg aagtgaaacc caagctatcc taccatatga tcaatactta tggaggttgc cagcgtacct tcaacagctt gacatggaaa gcaatggaaa gggtgccacc aaaaacggcc gaatggtgtc aacgcacact ggtcctatta tttttgggga agctggcacc aacggccaac atgcttttta tcaacttatt catcaaggga caaagttaat tccgtgcgac ttcatcggcg cgattcaaac tcaaaattat attggagaac atcaccgaat tttgatgagc aacttttttg ctcagacaga ggcactgatg attggtaaga cccctgagga ggttaaaagg gaactggaat ccgcgggagg aaagtctgag gatgagattc aattgttgat accacaaaaa acgttcactg ggggacgtcc aagcaactcc ttgttggtaa aggctctcac tccacgggca ttgggtgcta ttattgccat gtatgagcac aaagttcttg tccaaggagc tatttgggga attaacagct atgatcaatg gggtgtggaa ctggggaaat tacttgccaa gtccattctt ctacaacttc agcctggcca aaaggttaca aaccatgatt cttcaacaaa tggtctcatt gagctattca acgaacgctc tcacttgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for TrcrA.17127.a.B1.PS01519
| PURIFICATION DATe: | 6/6/2012 |
| CONCENTRATION: | 75.26mg/ml |
| OBSERVED MW: | 64kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 90 |
| PERCENT COVERAGE: | 59 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK DQYLKDLTVH LNESNAAPAN TSMAVASFNM PHEITRRMRP LGVDADTSLT SCPSWRRLQE LYEIHGSESI LKNFDECKDR FQRYSLEVDL RSSDKNFVFL DYSKTHINDE IKDVLFKLVE ERGIRAFMRA LFAGEKVNTA ENRSVLHIAL RNRSNRPIFV NGHDVMPLVN KVLEQMKKLS EKVRRGEWKG QSGKPIRHVV NIGIGGSDLG PMMACEALRP FSDRRISMHF VSNIDGTHLS EVLNLVDLES TLFIIASKTF TTQETITNAL SARNEFLKFL SSRGISEAGA VAKHFVXLST NAXKVKEFGX XXXNMXXFXX LGRWXILXXV XHXFXCHDFN WXRQFCGVVN WCTHHG
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa gatcaatacc tgaaggattt gacagttcac ttgaacgaaa gcaatgcggc acccgcgaat accagcatgg ctgtcgcatc tttcaatatg ccacacgaaa taacacgaag gatgaggcca cttggtgttg atgctgacac aagcttgact tcgtgcccct cctggcgtcg tcttcaagaa ttatacgaga tccacggaag cgagagcatt ttgaagaatt tcgacgaatg taaggatcgt ttccaacgat actcgcttga agtggatttg agaagcagtg acaaaaactt cgtttttttg gattactcaa agacacacat aaatgatgaa ataaaagacg ttctttttaa gttggttgaa gagagaggta ttcgagcgtt tatgcgggca ttgtttgctg gagagaaggt taatacggcc gaaaatcgtt cagtgcttca tattgctttg cgaaatcgaa gcaatcgtcc catttttgtg aatggacatg acgttatgcc cttggtaaac aaggtacttg aacaaatgaa gaaactatca gaaaaggtca ggagaggtga atggaaaggt cagtcaggaa aacctatccg ccatgttgtg aatattggga ttggaggcag tgatctcggt cccatgatgg catgtgaagc tttgaggcct ttttcagatc ggagaatatc catgcatttt gtttctaata ttgatgggac acacttgtct gaagtcttga atttggtcga cttggaatca actcttttta taattgccag caaaacattc actacacaag aaaccatcac gaacgctttg tccgcacgga atgagttttt gaagttttta agttctcggg gtatttcaga agctggtgct gttgcaaagc actttgttgn gctttcaaca aatgcgnaaa aagtcaaaga atttggtant gangangnna atatgtnnna nttttnggna ctgggtcggt ggnnnatact catngnggtc ngccatnggt ttntctgtca tgatttcaat tggntacgac aattttgtgg agttgttaac tggtgcacac atcatggatg ancacttnat caatgcaccc acggaaanca ntctccntat tattttggct ttggttggta tnnggtacaa taanttcttt ggaagtgaaa cccaagctat cctaccatat gatcaatact tatggaggtt gccagcgtac cttcaacagc ttgacatgga aagcaatgga aagggtgcca ccaaaaacgg ccgaatggtg tcaacgcaca ctggtcctat tatttttggg gaagctggca ccaacggcca acatgctttt tatcaactta ttcatcaagg gacaaagtta attccgtgcg acttcatcgg cgcgattcaa actcaaaatt atattggaga acatcaccga attttgatga gcaacttttt tgctcagaca gaggcactga tgattggtaa gacccctgag gaggttaaaa gggaactgga atccgcggga ggaaagtctg aggatgagat tcaattgttg ataccacaaa aaacgttcac tgggggacgt ccaagcaact ccttgttggt aaaggctctc actccacggg cattgggtgc tattattgcc atgtatgagc acaaagttct tgtccaagga gctatttggg gaattaacag ctatgatcaa tggggtgtgg aactggggaa attacttgcc aagtccattc ttctacaact tcagcctggc caaaaggtta caaaccatga ttcttcaaca aatggtctca ttgagc
Expressed Protein Sequence
MAHHHHHHMK DQYLKDLTVH LNESNAAPAN TSMAVASFNM PHEITRRMRP LGVDADTSLT SCPSWRRLQE LYEIHGSESI LKNFDECKDR FQRYSLEVDL RSSDKNFVFL DYSKTHINDE IKDVLFKLVE ERGIRAFMRA LFAGEKVNTA ENRSVLHIAL RNRSNRPIFV NGHDVMPLVN KVLEQMKKLS EKVRRGEWKG QSGKPIRHVV NIGIGGSDLG PMMACEALRP FSDRRISMHF VSNIDGTHLS EVLNLVDLES TLFIIASKTF TTQETITNAL SARNEFLKFL SSRGISEAGA VAKHFVALST NAEKVKEFGI DEENMFQFWD WVGGRYSLWS AIGLSVMISI GYDNFVELLT GAHIMDEHFI NAPTENNLPI ILALVGIWYN NFFGSETQAI LPYDQYLWRL PAYLQQLDME SNGKGATKNG RMVSTHTGPI IFGEAGTNGQ HAFYQLIHQG TKLIPCDFIG AIQTQNYIGE HHRILMSNFF AQTEALMIGK TPEEVKRELE SAGGKSEDEI QLLIPQKTFT GGRPSNSLLV KALTPRALGA IIAMYEHKVL VQGAIWGINS YDQWGVELGK LLAKSILLQL QPGQKVTNHD SSTNGLIELF NERSHL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG ATCAATACCT GAAGGATTTG ACAGTTCACT TGAACGAAAG CAATGCGGCA CCCGCGAATA CCAGCATGGC TGTCGCATCT TTCAATATGC CACACGAAAT AACACGAAGG ATGAGGCCAC TTGGTGTTGA TGCTGACACA AGCTTGACTT CGTGCCCCTC CTGGCGTCGT CTTCAAGAAT TATACGAGAT CCACGGAAGC GAGAGCATTT TGAAGAATTT CGACGAATGT AAGGATCGTT TCCAACGATA CTCGCTTGAA GTGGATTTGA GAAGCAGTGA CAAAAACTTC GTTTTTTTGG ATTACTCAAA GACACACATA AATGATGAAA TAAAAGACGT TCTTTTTAAG TTGGTTGAAG AGAGAGGTAT TCGAGCGTTT ATGCGGGCAT TGTTTGCTGG AGAGAAGGTT AATACGGCCG AAAATCGTTC AGTGCTTCAT ATTGCTTTGC GAAATCGAAG CAATCGTCCC ATTTTTGTGA ATGGACATGA CGTTATGCCC TTGGTAAACA AGGTACTTGA ACAAATGAAG AAACTATCAG AAAAGGTCAG GAGAGGTGAA TGGAAAGGTC AGTCAGGAAA ACCTATCCGC CATGTTGTGA ATATTGGGAT TGGAGGCAGT GATCTCGGTC CCATGATGGC ATGTGAAGCT TTGAGGCCTT TTTCAGATCG GAGAATATCC ATGCATTTTG TTTCTAATAT TGATGGGACA CACTTGTCTG AAGTCTTGAA TTTGGTCGAC TTGGAATCAA CTCTTTTTAT AATTGCCAGC AAAACATTCA CTACACAAGA AACCATCACG AACGCTTTGT CCGCACGGAA TGAGTTTTTG AAGTTTTTAA GTTCTCGGGG TATTTCAGAA GCTGGTGCTG TTGCAAAGCA CTTTGTTGCG CTTTCAACAA ATGCGGAAAA AGTCAAAGAA TTTGGTATTG ATGAAGAGAA TATGTTCCAA TTTTGGGACT GGGTCGGTGG CCGATACTCA TTGTGGTCCG CCATTGGTTT ATCTGTCATG ATTTCAATTG GGTACGACAA TTTTGTGGAG TTGTTAACTG GTGCACACAT CATGGATGAA CACTTTATCA ATGCACCCAC GGAAAACAAT CTCCCTATTA TTTTGGCTTT GGTTGGTATT TGGTACAATA ACTTCTTTGG AAGTGAAACC CAAGCTATCC TACCATATGA TCAATACTTA TGGAGGTTGC CAGCGTACCT TCAACAGCTT GACATGGAAA GCAATGGAAA GGGTGCCACC AAAAACGGCC GAATGGTGTC AACGCACACT GGTCCTATTA TTTTTGGGGA AGCTGGCACC AACGGCCAAC ATGCTTTTTA TCAACTTATT CATCAAGGGA CAAAGTTAAT TCCGTGCGAC TTCATCGGCG CGATTCAAAC TCAAAATTAT ATTGGAGAAC ATCACCGAAT TTTGATGAGC AACTTTTTTG CTCAGACAGA GGCACTGATG ATTGGTAAGA CCCCTGAGGA GGTTAAAAGG GAACTGGAAT CCGCGGGAGG AAAGTCTGAG GATGAGATTC AATTGTTGAT ACCACAAAAA ACGTTCACTG GGGGACGTCC AAGCAACTCC TTGTTGGTAA AGGCTCTCAC TCCACGGGCA TTGGGTGCTA TTATTGCCAT GTATGAGCAC AAAGTTCTTG TCCAAGGAGC TATTTGGGGA ATTAACAGCT ATGATCAATG GGGTGTGGAA CTGGGGAAAT TACTTGCCAA GTCCATTCTT CTACAACTTC AGCCTGGCCA AAAGGTTACA AACCATGATT CTTCAACAAA TGGTCTCATT GAGCTATTCA ACGAACGCTC TCACTTGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT