TrcrA.01625.a

S-adenosylmethionine synthetase

CENTER ID: TrcrA.01625.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01625.a.B1.GE35183 full length 1 394
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053506945.160

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVRQILFSS EHVSEGHPDK LCDQVSDAVL DACLASDPLS RVACETCSKT GMIMIFGEIT TNAVLDYQKV VRDAVKEIGF DDGEKGLDYR SCNLLIAIEH QSPEILQSLG DFDGEGLGAG DQGMMFGYAT DETETFMPLT YELSRGLAVK YSELRRNGTL PWARPDAKTQ VTVQYEYDSR EGRQLLTPKR VAVVLISAQH DADVKNETLR KDLMEKVIKA VIPAHMLDAD TKYHLNPSGR FVIGGPHGDA GLTGRKIIVD TYGGWGAHGG GAFSGKDPSK VDRSAAYAAR WIAKSLVAGG LARRCLVQLA YAIGVAEPLN IHIETYGTGK YDDGRILEIV TKNFRLRPYD IIKELDLRRP IYHMTSRFGH FGREDKSGRG GFTWEIPKKL DESC
NT Sequence
atgtctgttc gccaaattct tttttcctct gaacacgtga gtgagggcca tcctgacaag ctttgtgacc aagtgtcgga cgctgtgctg gatgcatgcc ttgcatcgga tcctctttca agggtggctt gtgaaacgtg ctccaagaca ggcatgatca tgatttttgg tgaaataacc acaaatgcgg tgctcgacta tcagaaggtg gtccgcgatg cggtgaagga gattggtttt gatgatggcg agaaggggtt ggactatcgt tcgtgcaacc ttctgattgc cattgagcac cagtctccag agatcctcca gagccttggg gactttgacg gcgagggact tggtgctggt gatcagggca tgatgtttgg ctacgcaact gacgagacgg agacatttat gccactcaca tacgagttgt cacgcggact tgctgtaaag tatagcgaac tacgccgtaa tgggacacta ccgtgggctc gtccggacgc gaagacgcag gtcactgtac agtatgagta cgactcacgt gaaggaagac agctgctgac tcctaagcgt gtggctgttg ttcttatctc ggcccaacac gatgctgatg tgaaaaacga gacactccgc aaggacctca tggaaaaggt catcaaggcc gtgatccctg cccacatgct tgatgccgat acaaagtacc acttgaatcc ttctggccga tttgtaattg gcggcccaca cggtgatgct ggccttactg gccgcaaaat cattgttgat acctacggcg gttggggcgc ccatgggggt ggcgccttct ctggcaagga cccttcaaag gtggaccgtt cggcggccta cgcggcccgc tggatcgcca agtcgcttgt cgccgggggt cttgcacgtc gttgtctcgt tcaactggcg tacgctattg gtgttgcaga accgctgaac attcacattg aaacctatgg caccggcaag tatgatgatg gacgcatcct ggagatcgtg acgaagaatt tcaggcttcg cccgtacgat attataaagg aactagatct tcgtcggccc atatatcaca tgacctcacg ctttggccac tttggacgcg aagacaagag tggcaggggt ggatttacct gggagatccc caagaagctc gatgagtcgt gctaa
Details for TrcrA.01625.a.B1.GE35183
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 95
Validated AA Sequence
MAHHHHHHHM SVRQILFSSE HVSEGHPDKL CDQVSDAVLD ACLASDPLSR VACETCSKTG MIMIFGEITT NAVLDYQKVV RDAVKEIGFD DGEKGLDYRS CNLLIAIEHQ SPEILQSLGD FDGEGLGAGD QGMMFGYATD ETETFMPLTY ELSRGLAVKY SELRRNGTLP WARPDAKTQV TVQYEYDSRE GRQLLTPKRV AVVLISAQHD ADVKNETLRK DLMEKVIKAV IPAHMLDADT KYHLNPSGRF VIGGPHGDAG LTGRKIIVDT YGGWGAHGGG AFSGKDPSKV DRSAAYAARW IAKSLVXXXL HVVVSFNWXT LXXLXXXXXX HXXXXHXXYX DXXHXGXRXX XSXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXX
Validated NT Sequence
ttttntnnnn tttaagaagg agatatacca tggctcacca ccaccaccac caccatatgt ctgttcgcca aattcttttt tcctctgaac acgtgagtga gggccatcct gacaagcttt gtgaccaagt gtctgacgct gtgctggatg catgccttgc atcggatcct ctttcaaggg tggcttgtga aacgtgctcc aagacaggca tgatcatgat ttttggtgaa ataaccacaa atgcggtgct cgactatcag aaggtggtcc gcgatgcggt gaaggaaatt ggttttgatg atggcgagaa ggggttggac tatcgctcgt gcaaccttct gattgccatt gagcaccagt ctccagagat cctccagagt cttggggact ttgacggcga gggacttggt gctggtgatc agggcatgat gtttggctac gcaactgacg agacggagac atttatgcca ctcacatacg agttgtcacg cggacttgct gtaaagtata gcgaactacg ccgtaatggg acactaccgt gggctcgtcc ggacgcgaag acgcaggtca ctgtacagta tgagtacgac tcacgtgaag gaagacagct gctgactcct aagcgtgtgg ctgttgttct tatctcggcc caacacgatg ctgatgtgaa aaacgagaca ctccgcaagg acctcatgga aaaggtcatc aaggccgtga tccctgccca catgcttgat gccgatacaa agtaccactt gaatccttct ggccgatttg taattggcgg cccacacggt gatgctggcc ttactggccg caaaatcatt gttgatacct acggtggttg gggcgcccat gggggtggcg ccttctctgg caaggaccct tcaaaggtgg accgttcggc ggcctacgcg gcccgctgga tcgccaagtc gcttgtcgnn nnnnncttgc acgtcgttgt ctcgttcaac tggngtacgc tantngngtt gcanaannnc tnannntnnc attnnannnt atngcaccng nnntatgang atngnannca tnnnggnnnn cgngncnaan nntcnnnnnn nncccnnncn annntnnnan nnnnnnnnan nntnnnnnnn nnnnnnnnnn nnnnnnnnnn nntnnnnnng nnnnntnncn nngnnntcnn nna
Expected Protein Sequence
MAHHHHHHMS VRQILFSSEH VSEGHPDKLC DQVSDAVLDA CLASDPLSRV ACETCSKTGM IMIFGEITTN AVLDYQKVVR DAVKEIGFDD GEKGLDYRSC NLLIAIEHQS PEILQSLGDF DGEGLGAGDQ GMMFGYATDE TETFMPLTYE LSRGLAVKYS ELRRNGTLPW ARPDAKTQVT VQYEYDSREG RQLLTPKRVA VVLISAQHDA DVKNETLRKD LMEKVIKAVI PAHMLDADTK YHLNPSGRFV IGGPHGDAGL TGRKIIVDTY GGWGAHGGGA FSGKDPSKVD RSAAYAARWI AKSLVAGGLA RRCLVQLAYA IGVAEPLNIH IETYGTGKYD DGRILEIVTK NFRLRPYDII KELDLRRPIY HMTSRFGHFG REDKSGRGGF TWEIPKKLDE SC
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg ttcgccaaat tcttttttcc tctgaacacg tgagtgaggg ccatcctgac aagctttgtg accaagtgtc ggacgctgtg ctggatgcat gccttgcatc ggatcctctt tcaagggtgg cttgtgaaac gtgctccaag acaggcatga tcatgatttt tggtgaaata accacaaatg cggtgctcga ctatcagaag gtggtccgcg atgcggtgaa ggagattggt tttgatgatg gcgagaaggg gttggactat cgttcgtgca accttctgat tgccattgag caccagtctc cagagatcct ccagagcctt ggggactttg acggcgaggg acttggtgct ggtgatcagg gcatgatgtt tggctacgca actgacgaga cggagacatt tatgccactc acatacgagt tgtcacgcgg acttgctgta aagtatagcg aactacgccg taatgggaca ctaccgtggg ctcgtccgga cgcgaagacg caggtcactg tacagtatga gtacgactca cgtgaaggaa gacagctgct gactcctaag cgtgtggctg ttgttcttat ctcggcccaa cacgatgctg atgtgaaaaa cgagacactc cgcaaggacc tcatggaaaa ggtcatcaag gccgtgatcc ctgcccacat gcttgatgcc gatacaaagt accacttgaa tccttctggc cgatttgtaa ttggcggccc acacggtgat gctggcctta ctggccgcaa aatcattgtt gatacctacg gcggttgggg cgcccatggg ggtggcgcct tctctggcaa ggacccttca aaggtggacc gttcggcggc ctacgcggcc cgctggatcg ccaagtcgct tgtcgccggg ggtcttgcac gtcgttgtct cgttcaactg gcgtacgcta ttggtgttgc agaaccgctg aacattcaca ttgaaaccta tggcaccggc aagtatgatg atggacgcat cctggagatc gtgacgaaga atttcaggct tcgcccgtac gatattataa aggaactaga tcttcgtcgg cccatatatc acatgacctc acgctttggc cactttggac gcgaagacaa gagtggcagg ggtggattta cctgggagat ccccaagaag ctcgatgagt cgtgctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat