TrcrA.01593.a

cdc2-related kinase, putative,cell division control protein, putative

CENTER ID: TrcrA.01593.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01593.a.B1.GE35181 full length 1 595
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053510329.70
RefSeq: XP_821184.1
UniProt: Q4E3K6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAVEGSPSS KEAARRQNPK KRVLCYEFDP ETDVIGRGAY GEVYKATLVS PSDNGTGTVA EAQDKFVALK RTLCADKEEG IPASTIREIM VLREISKAMK NGAGKEGSPE SFGCENVVKL YDVFMDKSTV YMASEFCEAG DLSCYLKTLP LRRLSDGQQY RQWMWDLLSG LCFLHRKGFS HRDLKPQNLV LKAIPSDPPS PEASREGGSR KEPKLTPQYK LKITDFGLSR VEGIPVKKYQ HEAVTLWYRS PDILLGNTNY NYTADMWSAG CIIAEVASGN AFFRGKNNRE QLKSIFSRLG LPTENNFPSI KQYALYETFF TGADSIQKGQ EEKEGNVATS YAAWFENVKE KLNHYFTRYG SVDIVGEDGV DLVARLLAYE PGRRLTTEEA IQHPFFHTVS HGILSTVGPN APPTAATSST THDANEILST SQEDTQGAAS HCSLAAFGVQ SQPPLPTQPQ EEKLEGVRGM IPTEVGGGNN GHAPLAGGQK EAHAVDEKDN PSKETDKSLE EGNNPGVTER SKPVESLNFK DRKKRGISTK LTSRPSMGVV CAERTKNSFS VKGSRVEEAP AYTRGCGQVP KSKAKVAITR ASKQL
NT Sequence
atggccgctg tggaaggatc gccatcttcc aaagaggcgg cgaggcgaca aaatcccaaa aagcgtgtgt tgtgttatga gtttgacccg gaaacggatg tgattggaag gggtgcatac ggagaggttt acaaggccac tctcgtgagt ccgtcagaca atggtacggg tacggtagcc gaggcgcagg acaagtttgt tgccctaaaa cgcacattgt gcgcagacaa ggaagaggga attcctgcct ccaccattcg agagatcatg gtgctgagag agatcagcaa ggcgatgaaa aatggggcag gaaaggaagg atctcccgaa tcatttggtt gtgaaaatgt cgttaaactt tacgacgtct ttatggacaa aagtacggtc tacatggcct ctgagttctg cgaggctggg gatttgtcgt gttacctcaa gacactcccg ctgcgtcgtc tctccgatgg ccagcagtac cgccaatgga tgtgggactt gcttagtggt ctgtgcttcc tgcataggaa gggattttcg caccgagact taaagccgca gaaccttgtg ctgaaggcga taccgagcga cccgccatcc ccagaagcct ctcgtgaagg tggatccagg aaggagccga agctgacgcc gcaatacaaa cttaagatca cggactttgg gctctccaga gttgagggga tccctgtaaa aaaatatcaa cacgaggctg tcaccctgtg gtaccgtagt cctgatatct tgctaggaaa cacaaactac aattacaccg ctgacatgtg gagtgccggc tgcatcattg ccgaagttgc cagtggcaac gctttttttc ggggtaaaaa taatcgtgag cagttgaaaa gcatcttttc ccgtttagga ttgccaacgg agaacaattt tcctagtata aagcagtacg cgttgtatga aacttttttt accggcgcag atagtattca aaagggtcag gaggaaaaag aaggcaacgt tgcgacgtcg tatgccgcct ggtttgaaaa cgtaaaggag aaattgaatc attacttcac taggtatgga tctgtggata ttgtgggcga agacggtgtg gatcttgttg cacgcctgtt ggcatatgag ccagggagac gcctgacaac agaggaagcc attcagcatc cttttttcca tacggtctct cacggcattt tgtcgacagt tgggccgaat gcacccccga ctgccgccac ctcgtctacg acgcacgatg caaacgaaat actcagcact tctcaggaag acacccaagg cgctgcgtca cattgctctt tggctgcatt tggtgtacag agccagccgc ctcttccgac gcagcctcag gaagaaaaat tggagggggt cagggggatg attcccacgg aagtcggagg cggaaacaac ggccacgcac ccctggcagg tggtcaaaaa gaagcacatg cggtggacga aaaggataac ccctctaaag aaactgacaa atctctggaa gaaggaaata atcctggagt gacggagaga agcaaacctg ttgaatctct caacttcaaa gatcgcaaaa agaggggaat ttctacaaag ttgacctcac gaccatccat gggcgttgtg tgtgctgaac ggacaaaaaa ctctttctca gtgaaaggat ctcgtgtgga agaggcacca gcatatacca gaggctgcgg ccaagtccct aaatcgaaag cgaaggttgc tataactcgc gctagcaaac aactttag
Details for TrcrA.01593.a.B1.GE35181
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 66kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 40
Validated AA Sequence
MAHHHHHHMA AVEGSPSSKE AARRENPKKR VLCYEFDPET DVIGRGAYGE VYKATLVSPS GNGTGTVAEA QDKFVALKRT LCADKEEGIP ASTIREIMVL REISKAMKNG AGKEGSPESF GCENVVKLYD VFMDKSTVYM ASEFCEAGDL SCYLKTLPLR RLSDGQQYRQ WMWDLLSGLC FLHRKGFSHR DLKPQNLVLK AIPSDPPSPE ASREGGSXKE PKLTPQYKLK ITDFGLSRVE GSL
Validated NT Sequence
attttgtnnn ntttaagaag gagatatacc atggctcacc accaccacca ccatatggcc gctgtggaag gatcgccatc ttccaaagag gcggcgaggc gagaaaatcc caaaaagcgt gtgttgtgtt atgagtttga cccggaaacg gatgtgattg gaaggggtgc atacggagag gtttacaaag ccactctcgt gagtccgtca ggcaatggta cgggtacggt agccgaggcg caggacaagt ttgttgccct aaaacgcaca ttgtgcgcag acaaggaaga gggaattcct gcctccacca ttcgggagat catggtgctg agagagatca gcaaggcgat gaaaaatggg gcaggaaagg aaggatctcc cgaatcattt ggttgtgaaa atgtcgttaa actttacgac gtctttatgg acaaaagtac ggtctacatg gcctctgagt tctgcgaggc tggggatttg tcgtgttacc tcaagacact cccgctgcgt cgtctctccg atggccagca gtaccgccaa tggatgtggg acttgcttag tggtctgtgc ttcctgcata ggaagggatt ttcgcaccga gacttaaagc cgcagaacct tgtgctgaag gcgataccga gcgacccgcc atccccagaa gcctctcgtg aaggtggatc cnggaaggag ccgaagctga cgccgcaata caaacttaag atcacggact ttgggctctc cagagttgag ggntccctgt aaaaaatatc aacacgannt gtcaccctgt ggtaccgtag tccnganatc tngctngaan cncaaactan nnncacngct gacntgtnnn tncnntgcat nntncnantn cnnngnangn tttttnngnn aaannancnn nnnnnntnaa annatcntnn nnnntnnnnn nnnnnaannn nnnntnncnn nnnnnnaann nntnnnntnn nnnnnnnnnn ag
Expected Protein Sequence
MAHHHHHHMA AVEGSPSSKE AARRQNPKKR VLCYEFDPET DVIGRGAYGE VYKATLVSPS DNGTGTVAEA QDKFVALKRT LCADKEEGIP ASTIREIMVL REISKAMKNG AGKEGSPESF GCENVVKLYD VFMDKSTVYM ASEFCEAGDL SCYLKTLPLR RLSDGQQYRQ WMWDLLSGLC FLHRKGFSHR DLKPQNLVLK AIPSDPPSPE ASREGGSRKE PKLTPQYKLK ITDFGLSRVE GIPVKKYQHE AVTLWYRSPD ILLGNTNYNY TADMWSAGCI IAEVASGNAF FRGKNNREQL KSIFSRLGLP TENNFPSIKQ YALYETFFTG ADSIQKGQEE KEGNVATSYA AWFENVKEKL NHYFTRYGSV DIVGEDGVDL VARLLAYEPG RRLTTEEAIQ HPFFHTVSHG ILSTVGPNAP PTAATSSTTH DANEILSTSQ EDTQGAASHC SLAAFGVQSQ PPLPTQPQEE KLEGVRGMIP TEVGGGNNGH APLAGGQKEA HAVDEKDNPS KETDKSLEEG NNPGVTERSK PVESLNFKDR KKRGISTKLT SRPSMGVVCA ERTKNSFSVK GSRVEEAPAY TRGCGQVPKS KAKVAITRAS KQL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg ctgtggaagg atcgccatct tccaaagagg cggcgaggcg acaaaatccc aaaaagcgtg tgttgtgtta tgagtttgac ccggaaacgg atgtgattgg aaggggtgca tacggagagg tttacaaggc cactctcgtg agtccgtcag acaatggtac gggtacggta gccgaggcgc aggacaagtt tgttgcccta aaacgcacat tgtgcgcaga caaggaagag ggaattcctg cctccaccat tcgagagatc atggtgctga gagagatcag caaggcgatg aaaaatgggg caggaaagga aggatctccc gaatcatttg gttgtgaaaa tgtcgttaaa ctttacgacg tctttatgga caaaagtacg gtctacatgg cctctgagtt ctgcgaggct ggggatttgt cgtgttacct caagacactc ccgctgcgtc gtctctccga tggccagcag taccgccaat ggatgtggga cttgcttagt ggtctgtgct tcctgcatag gaagggattt tcgcaccgag acttaaagcc gcagaacctt gtgctgaagg cgataccgag cgacccgcca tccccagaag cctctcgtga aggtggatcc aggaaggagc cgaagctgac gccgcaatac aaacttaaga tcacggactt tgggctctcc agagttgagg ggatccctgt aaaaaaatat caacacgagg ctgtcaccct gtggtaccgt agtcctgata tcttgctagg aaacacaaac tacaattaca ccgctgacat gtggagtgcc ggctgcatca ttgccgaagt tgccagtggc aacgcttttt ttcggggtaa aaataatcgt gagcagttga aaagcatctt ttcccgttta ggattgccaa cggagaacaa ttttcctagt ataaagcagt acgcgttgta tgaaactttt tttaccggcg cagatagtat tcaaaagggt caggaggaaa aagaaggcaa cgttgcgacg tcgtatgccg cctggtttga aaacgtaaag gagaaattga atcattactt cactaggtat ggatctgtgg atattgtggg cgaagacggt gtggatcttg ttgcacgcct gttggcatat gagccaggga gacgcctgac aacagaggaa gccattcagc atcctttttt ccatacggtc tctcacggca ttttgtcgac agttgggccg aatgcacccc cgactgccgc cacctcgtct acgacgcacg atgcaaacga aatactcagc acttctcagg aagacaccca aggcgctgcg tcacattgct ctttggctgc atttggtgta cagagccagc cgcctcttcc gacgcagcct caggaagaaa aattggaggg ggtcaggggg atgattccca cggaagtcgg aggcggaaac aacggccacg cacccctggc aggtggtcaa aaagaagcac atgcggtgga cgaaaaggat aacccctcta aagaaactga caaatctctg gaagaaggaa ataatcctgg agtgacggag agaagcaaac ctgttgaatc tctcaacttc aaagatcgca aaaagagggg aatttctaca aagttgacct cacgaccatc catgggcgtt gtgtgtgctg aacggacaaa aaactctttc tcagtgaaag gatctcgtgt ggaagaggca ccagcatata ccagaggctg cggccaagtc cctaaatcga aagcgaaggt tgctataact cgcgctagca aacaactttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat