SSGCID: Target Page

TrcrA.01554.b

cyclophilin, putative

CENTER ID:	TrcrA.01554.b
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.01554.b.B1.GE35179	full length		1	325

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053511217.120
RefSeq:	XP_818332.1
UniProt:	Q4DVC9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNLKATSRLG CTLTQGSPAS GTRYASHSRP IERTSCWMEL ACFGGFPKVP SLSGSMVQQR QDLLASTKGA GEPEIILRIE FELFDDESPK ACANFRHLCA GQSTSRKGQT YCYQGLTPCY RGTYFHKIIP AFCVQGGDLT MRVNKGANHF SSFGWGWFSD ENKRRRLNEV GLLLMANNGP NSNGSQFFIT TSDSEEKALN GRHVCFGRVV RGLDEFLREV APYGEINGNP SRFVVVVDCG VGPLPETLGI TTSNSWAEEQ KRHKAEGDNA NEGVGFEDGA ATVAGAANAA ASVEEEEEKG KEKELEETTT AMEANKQPPA MNGTS

NT Sequence

atgaacctta aggcaaccag ccgccttgga tgcacactca cacaaggatc tcctgcctcc gggacacgct atgctagtca ttcacgtccc atagaacgca cgtcatgttg gatggagctc gcatgttttg gcggctttcc aaaggttccg tctctctcag gctcgatggt gcagcagcgc caggatcttt tggcgtctac taaaggcgcc ggtgaacctg aaatcatcct tcgtattgag tttgaattat ttgatgatga atcacccaaa gcatgtgcga atttccgtca tttatgtgcg gggcagtcca ccagtcgaaa agggcagacg tactgttacc aaggtctcac gccatgttat cgtgggacgt actttcataa aattataccc gcgttttgcg ttcagggtgg ggacttgaca atgcgtgtta acaaaggtgc gaatcacttc tccagttttg gttggggttg gtttagtgac gaaaacaaac gacggcgcct gaatgaggtg ggtcttcttc ttatggccaa caatggaccc aacagcaacg gctcgcagtt cttcatcacc acctctgatt cggaagaaaa ggcactcaat ggccggcatg tttgctttgg tcgggtggtg cgtggcctcg atgagttctt gagggaagtg gcaccgtacg gtgagatcaa cggcaacccc tcgcgatttg tagttgtcgt tgactgcggc gtgggcccac tgccggagac actgggcatc accaccagca acagctgggc agaggagcaa aaaagacaca aggccgaggg cgataatgcc aatgaaggag tggggtttga agatggtgcg gcgacagttg caggtgctgc aaacgctgcc gcatcggtag aagaagaaga agaaaaagga aaagaaaagg aacttgaaga gactacaacc gccatggaag ccaacaagca accaccggct atgaatggca caagttaa

Details for TrcrA.01554.b.B1.GE35179

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	36kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN LKATSRLGCT LTQGSPASGT RYASHSRPIE RTSCWMELAC FGGFPKVPSL SGSMVQQRQD LLASTKGAGE PEIILRIEFE LFDDESPKAC ANFRHLCAGQ STSRKGQTYC YQGLTPCYRG TYFHKIIPAF CVQGGDLTMR VNKGANHFSS FGWGWFSDEN KRRRLNEVGL LLMANNGPNS NGSQFFITTS DSEEKALNGR HVCFGRVVRG LDEFLREVAP YGEINGNPSR FVVVVDCGVG PLPETLGITT SNSWAEEQKR HKAEGDNANE GVGFEDGAAT VAGAANAAAS VEEEEEKGKE KELEETTTAM EANKQPPAMN GTS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc ttaaggcaac cagccgcctt ggatgcacac tcacacaagg atctcctgcc tccgggacac gctatgctag tcattcacgt cccatagaac gcacgtcatg ttggatggag ctcgcatgtt ttggcggctt tccaaaggtt ccgtctctct caggctcgat ggtgcagcag cgccaggatc ttttggcgtc tactaaaggc gccggtgaac ctgaaatcat ccttcgtatt gagtttgaat tatttgatga tgaatcaccc aaagcatgtg cgaatttccg tcatttatgt gcggggcagt ccaccagtcg aaaagggcag acgtactgtt accaaggtct cacgccatgt tatcgtggga cgtactttca taaaattata cccgcgtttt gcgttcaggg tggggacttg acaatgcgtg ttaacaaagg tgcgaatcac ttctccagtt ttggttgggg ttggtttagt gacgaaaaca aacgacggcg cctgaatgag gtgggtcttc ttcttatggc caacaatgga cccaacagca acggctcgca gttcttcatc accacctctg attcggaaga aaaggcactc aatggccggc atgtttgctt tggtcgggtg gtgcgtggcc tcgatgagtt cttgagggaa gtggcaccgt acggtgagat caacggcaac ccctcgcgat ttgtagttgt cgttgactgc ggcgtgggcc cactgccgga gacactgggc atcaccacca gcaacagctg ggcagaggag caaaaaagac acaaggccga gggcgataat gccaatgaag gagtggggtt tgaagatggt gcggcgacag ttgcaggtgc tgcaaacgct gccgcatcgg tagaagaaga agaagaaaaa ggaaaagaaa aggaacttga agagactaca accgccatgg aagccaacaa gcaaccaccg gctatgaatg gcacaagttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat