SSGCID: Target Page

TrcrA.01530.a

peroxisomal enoyl-coa hydratase, putative

CENTER ID:	TrcrA.01530.a
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.01530.a.B1.GE35175	full length		1	313

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053511277.210
RefSeq:	XP_821889.1
UniProt:	Q4E5H2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFRRCLRLCD FSVLLATFEP FELYGSKDTG ICEMVVNNKK NSLNLLDRSF FSSLLKATNY LSSELNGVAR VGILTSKEGT PFSAGLDLKQ LQELVKGPSS SATTGEKAVN SFGMPAMQFQ RQHAEIRLFQ SSVSSLARCS IPIICAIDGY CIGGATSIIT ACDFRYATEK AFFSVKEAQV GLAADLGVLQ RLPRIVGEGR ARELVYTARS FNGKEAKEMG LVEEVFESRE EMIEAVRKTA TLIASNSPLA VQGSKLLMNR QTEPDVERGL EYTASWSAGN VACDDVLEAA AAFAKKSPPL FKNYVVNTSS TVT

NT Sequence

atgtttcgtc ggtgcttacg tctttgcgac ttctctgttc ttttggctac ttttgagcct tttgaactat atgggagcaa ggatacgggg atttgtgaaa tggtagtaaa caataagaag aattctctga atctattgga tcgatcattt ttttcttctt tattgaaagc gacaaattat ctttcaagtg aacttaatgg ggttgcacgg gtagggattt taacgtcaaa agagggaact cctttttctg caggtttaga tttgaagcaa ttgcaggagt tagtaaaggg gccttcgagc tccgctacga cgggtgaaaa agcagtaaat tcttttggga tgccggcaat gcagtttcaa cgacaacatg cggaaattcg actgtttcag tcgagtgttt cttcattggc tcgttgttct attcccatta tatgtgcaat tgatggttat tgcatcggtg gagctacatc tattattacg gcttgtgact tccgttacgc cactgaaaag gctttttttt ccgtaaaaga ggcgcaagtt ggtttagcgg ctgatctcgg tgtccttcaa cggctaccac gcattgtagg ggagggccgc gcacgggaat tggtatacac ggcccgctcc tttaatggaa aggaggctaa ggaaatgggt cttgtggagg aggtttttga gagtcgggaa gaaatgatcg aggcggttcg caaaaccgcc actttgattg catcaaactc tcctttagct gttcaaggat caaagttgtt aatgaatcgt cagacggagc cagatgtgga aagagggcta gaatataccg catcgtggag tgcaggaaat gtggcatgtg atgacgtttt ggaggcggct gcggcatttg cgaaaaaaag cccaccactt tttaagaatt atgtggtaaa caccagttct actgtaactt ga

Details for TrcrA.01530.a.B1.GE35175

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	90
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMF RRCLRLCDFS VLLATFEPFE LYGSKDTGIC EMVVNNKKNS LNLLDRSFFS SLLKATNYLS SELNGVARVG ILTSKEGTPF SAGLDLKQLQ ELVKGPSSSA TTGEKAVNSF GMPAMQFQRQ HAEIRLFQSS VSSLARCSIP IICAIDGYCI GGATSIITAC DFRYATEKAF FSVKEAQVGL AADLGVLQRL PRIVGEGRAR ELVYTARSFN GTEAKEMGLV EEVFESREKM IEAVRKSATL IASNSPLAVQ GSKLLMNRQT EPDVERGLEY TASWXQXMWH VMTFWXXLXH LRKKAHHFLX IMW

Validated NT Sequence

ttntnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgtttcgt cggtgcttac gtctttgcga cttctctgtt cttttggcta cttttgagcc ttttgaacta tatgggagca aggatacggg gatttgtgaa atggtagtaa acaataagaa gaattctctg aatctattgg atcgatcatt tttttcttct ttattgaaag cgacaaatta tctttcaagt gaacttaatg gggttgcacg ggtagggatt ttaacgtcaa aagagggaac tcctttttct gcaggtttag atttgaagca attgcaggag ttagtaaagg ggccttcgag ctccgctacg acgggtgaaa aagcagtaaa ttcttttggg atgccggcaa tgcagtttca acgacaacat gcggaaattc gactgtttca gtcgagtgtt tcttcattgg ctcgttgttc tattcccatt atatgtgcaa ttgatggtta ttgcatcggt ggagctacat ctattattac ggcttgtgac ttccgttacg ccactgaaaa ggcttttttt tccgtaaaag aggcgcaagt tggtttagcg gctgatctcg gtgtccttca acggctacca cgcattgtag gggagggccg cgcacgggaa ttggtataca cggcccgctc ctttaatgga acggaggcta aggaaatggg tcttgtggag gaggtttttg agagtcggga aaaaatgatc gaggcggttc gcaaaagcgc cactttgatt gcatcaaact cacctttagc tgttcaggga tcaaagttgt taatgaatcg tcagacggag ccagatgtgg aaagagggct agaatatacc gcatcgtggn tgcagnaaat gtggcatgtg atgacgtttt ggagnnggct gcngcatttg cgaaaaaaag cccaccactt tttganaatt atgtggtaaa nnncagtnct actgnnnttn agtgannann nnnnancnnn nnnncccccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn g

Expected Protein Sequence

MAHHHHHHMF RRCLRLCDFS VLLATFEPFE LYGSKDTGIC EMVVNNKKNS LNLLDRSFFS SLLKATNYLS SELNGVARVG ILTSKEGTPF SAGLDLKQLQ ELVKGPSSSA TTGEKAVNSF GMPAMQFQRQ HAEIRLFQSS VSSLARCSIP IICAIDGYCI GGATSIITAC DFRYATEKAF FSVKEAQVGL AADLGVLQRL PRIVGEGRAR ELVYTARSFN GKEAKEMGLV EEVFESREEM IEAVRKTATL IASNSPLAVQ GSKLLMNRQT EPDVERGLEY TASWSAGNVA CDDVLEAAAA FAKKSPPLFK NYVVNTSSTV T

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttc gtcggtgctt acgtctttgc gacttctctg ttcttttggc tacttttgag ccttttgaac tatatgggag caaggatacg gggatttgtg aaatggtagt aaacaataag aagaattctc tgaatctatt ggatcgatca tttttttctt ctttattgaa agcgacaaat tatctttcaa gtgaacttaa tggggttgca cgggtaggga ttttaacgtc aaaagaggga actccttttt ctgcaggttt agatttgaag caattgcagg agttagtaaa ggggccttcg agctccgcta cgacgggtga aaaagcagta aattcttttg ggatgccggc aatgcagttt caacgacaac atgcggaaat tcgactgttt cagtcgagtg tttcttcatt ggctcgttgt tctattccca ttatatgtgc aattgatggt tattgcatcg gtggagctac atctattatt acggcttgtg acttccgtta cgccactgaa aaggcttttt tttccgtaaa agaggcgcaa gttggtttag cggctgatct cggtgtcctt caacggctac cacgcattgt aggggagggc cgcgcacggg aattggtata cacggcccgc tcctttaatg gaaaggaggc taaggaaatg ggtcttgtgg aggaggtttt tgagagtcgg gaagaaatga tcgaggcggt tcgcaaaacc gccactttga ttgcatcaaa ctctccttta gctgttcaag gatcaaagtt gttaatgaat cgtcagacgg agccagatgt ggaaagaggg ctagaatata ccgcatcgtg gagtgcagga aatgtggcat gtgatgacgt tttggaggcg gctgcggcat ttgcgaaaaa aagcccacca ctttttaaga attatgtggt aaacaccagt tctactgtaa cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat