SSGCID: Target Page

TrcrA.01515.a

cell division protein kinase 2

CENTER ID:	TrcrA.01515.a
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.01515.a.B1.GE35174	full length		1	311

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053506677.20
RefSeq:	XP_804054.1
UniProt:	Q4CPK2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTLTTLPSR PLSSNAREQF ERYNRMDILG EGTYGVVYRA VDKITGQIVA LKKVRLDRTE EGIPQTALRE VSILQEIHHP NVVNLLDVIC TDGKLYLIFE YVDYDLKKAI EKRGCTFTGV TLKKLVYQLL DGLFFCHRHR IVHRDLKPAN ILITSDNVLK LADFGLARTF QIPMHTYTHE VVTLWYRAPE ILLGEKHYTP AVDIWSVGCI FAELARGKVI FRGDSEIGQL FEIFQILGTP MDNEGSWPGV SSLPDYRDVF PRWAGKPLAQ VIPQLDSEAI DLLSRMLKYS PAERISAKEA LQHSWFSEIR T

NT Sequence

atgactactc tcactacctt gccgagccgg ccgctttctt caaacgcccg agagcagttt gaacgataca atcgtatgga tattcttgga gagggaacat atggtgttgt ttatcgtgca gtcgacaaaa tcacaggaca aattgtcgcc ttaaagaaag tgcgattgga tcgtacagaa gaaggaattc ctcaaacggc tcttcgagaa gtttctattt tgcaggaaat tcatcatcca aatgttgtga acttgcttga tgttatctgc actgatggta agctttattt gatttttgag tatgtggatt acgatctcaa aaaggcaatt gaaaagaggg gttgtacttt taccggtgtg acattaaaga aacttgttta tcagcttctt gatggtctat ttttttgcca cagacatcga attgtgcatc gtgacctcaa acctgcaaat attcttatta cgagtgataa tgttcttaag ctcgccgatt ttggtttggc gcgcacattt caaattccca tgcatactta tacccatgaa gttgtcacgt tatggtatcg agcccccgag atcttattgg gagaaaaaca ctatacccct gccgtggata tttggagtgt tggatgtatt tttgcagaat tggctcgagg taaggttatt tttcgcggag acagtgaaat tggccagctt tttgagatat ttcaaattct tgggacgcca atggacaatg agggatcatg gccgggagta tcttctcttc ctgactaccg cgatgtgttt ccccgttggg caggaaaacc acttgctcag gtgattccgc agctcgacag tgaggcaatt gatcttcttt cacgaatgct caaatatagc cccgcagaac ggatatctgc aaaagaggca ctgcagcatt cgtggttcag tgaaattaga acgtag

Details for TrcrA.01515.a.B1.GE35174

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	36kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT TLTTLPSRPL SSNAREQFER YNRMDILGEG TYGVVYRAVD KITGQIVALK KVRLDRTEEG IPQTALREVS ILQEIHHPNV VNLLDVICTD GKLYLIFEYV DYDLKKAIEK RGCTFTGVTL KKLVYQLLDG LFFCHRHRIV HRDLKPANIL ITSDNVLKLA DFGLARTFQI PMHTYTHEVV TLWYRAPEIL LGEKHYTPAV DIWSVGCIFA ELARGKVIFR GDSEIGQLFE IFQILGTPMD NEGSWPGVSS LPDYRDVFPR WAGKPLAQVI PQLDSEAIDL LSRMLKYSPA ERISAKEALQ HSWFSEIRT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta ctctcactac cttgccgagc cggccgcttt cttcaaacgc ccgagagcag tttgaacgat acaatcgtat ggatattctt ggagagggaa catatggtgt tgtttatcgt gcagtcgaca aaatcacagg acaaattgtc gccttaaaga aagtgcgatt ggatcgtaca gaagaaggaa ttcctcaaac ggctcttcga gaagtttcta ttttgcagga aattcatcat ccaaatgttg tgaacttgct tgatgttatc tgcactgatg gtaagcttta tttgattttt gagtatgtgg attacgatct caaaaaggca attgaaaaga ggggttgtac ttttaccggt gtgacattaa agaaacttgt ttatcagctt cttgatggtc tatttttttg ccacagacat cgaattgtgc atcgtgacct caaacctgca aatattctta ttacgagtga taatgttctt aagctcgccg attttggttt ggcgcgcaca tttcaaattc ccatgcatac ttatacccat gaagttgtca cgttatggta tcgagccccc gagatcttat tgggagaaaa acactatacc cctgccgtgg atatttggag tgttggatgt atttttgcag aattggctcg aggtaaggtt atttttcgcg gagacagtga aattggccag ctttttgaga tatttcaaat tcttgggacg ccaatggaca atgagggatc atggccggga gtatcttctc ttcctgacta ccgcgatgtg tttccccgtt gggcaggaaa accacttgct caggtgattc cgcagctcga cagtgaggca attgatcttc tttcacgaat gctcaaatat agccccgcag aacggatatc tgcaaaagag gcactgcagc attcgtggtt cagtgaaatt agaacgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t