TrcrA.01510.b

protein kinase A catalytic subunit

CENTER ID: TrcrA.01510.b
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01510.b.B1.GE35173 full length 1 387
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053508461.310
RefSeq: XP_821623.1
UniProt: Q4E4S9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSFPHFVSF SYMCIYMYTS TNGHFSFLLK EPTQIILTVF LKNRHWRIAK RETTVINKMS GEANYEKPDT SNWKLADFEM GDTLGTGSFG RVRIAKLKGT NDYYAVKCLK KREILKMKQV QHISQEKQIL MELSHPFIVN MMCSFQDDRR VYFVLEFVVG GEMFTHLRSA GRFPNDVAKF YHAEIVLAFE YLHSKDIIYR DLKPENLLLD SKGHVKVTDF GFAKKVPERT FTLCGTPEYL APEVIQSKGH GKAVDWWTMG VLLYEFIAGY PPFYDDTPFR TYEKILSGRF KFPSWFDARA RDLVKGLLQT DHTKRLGTLK GGVADVKNHP YFHGANWEKL YARYYPAPIP VKAKSPGDTS NFERYPESQE DRAVPLTATQ QAEFIGF
NT Sequence
atgacttcat ttccccactt tgtttctttt tcttatatgt gtatatatat gtatacatct acgaatgggc atttttcctt ccttctaaaa gagcccacac agataatttt aacagtattt ttgaaaaata ggcactggcg aattgccaag agggagacga cagttataaa caaaatgtca ggggaggcaa attatgaaaa gccggacacc tcaaattgga agttagcaga ctttgagatg ggcgacacgt tagggacggg ttcttttggt cgcgtgcgca ttgctaaact gaagggcacc aatgactatt atgcggtgaa gtgcttgaag aagcgggaga ttttaaagat gaagcaggtc caacacatca gccaagaaaa gcaaatttta atggagcttt cacacccgtt cattgtgaac atgatgtgtt cctttcagga tgatcgccgg gtgtactttg tgttggagtt tgtggtgggc ggggaaatgt ttactcacct tcgctccgcc ggtcggtttc caaacgatgt agcaaagttc taccacgcgg agattgtgct tgccttcgaa tacctgcact cgaaggacat catctaccgt gacctgaagc cggagaacct tctgctggac agcaagggcc acgtgaaggt gactgacttt gggtttgcga agaaggtgcc ggaacgtacc ttcacgctgt gtgggacacc ggagtacctt gcgcccgagg tgattcagag caagggtcat gggaaggccg tggactggtg gacgatgggc gttctgctgt acgaatttat tgctgggtat cctccgtttt acgacgacac gccgttccgc acgtacgaga agatcctctc tgggcgcttc aagtttccga gttggtttga tgcgcgtgcg cgggatcttg tgaaggggct tctgcaaacg gaccacacga agcgtttggg cacactcaag ggcggcgtag cggatgtgaa gaatcacccg tactttcacg gtgccaactg ggagaagttg tatgcgcgat actaccctgc cccgatccct gtgaaggcaa agagccctgg cgacacgagt aactttgagc gttaccctga aagccaggaa gatcgagcgg tgccgctaac cgctacgcaa caggcggagt ttattgggtt ctaa
Details for TrcrA.01510.b.B1.GE35173
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 94
Validated AA Sequence
MAHHHHHHMT SFPHFVSFSY MCIYMYTSTN GHFSFLLKEP TQIILTVFLK NRHWRIAKRE TTVINKMSGE ANYEKPDTSN WKLADFEMGD TLGTGSFGRV RIAKLKGTND YYAVKCLKKR EILKMKQVQH ISQEKQILME LSHPFIVNMM CSFQDDCRVY FVLEFVVGGE MFTHLRSAGR FPNDVAKFYH AEIVLAFEYL HSKDIIYRDL KPENLLLDSK GHVKVTDFGF AKKVPERTFT LCGTPEYLAP XVIQSKGHGK AVDWWTMGVL LYEFIAGYPP FYDDXPFRTY EKILSGRFKF PSWFDARARX LVXGLLXXHT XRXGXLXGXX XXXNXXXXXX XXXXXXXXIL XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
attttgttta actttaagaa ggaganatac catggctcac caccaccacc accatatgac ttcatttccc cactttgttt ctttttctta tatgtgtata tatatgtata catctacgaa tgggcatttt tccttccttc taaaagagcc cacacagata attttaacag tatttttgaa aaataggcac tggcgaattg ccaagaggga gacgacagtt ataaacaaaa tgtcagggga ggcaaattat gaaaagccgg acacctcaaa ttggaagtta gcagactttg agatgggcga cacgttaggg acgggttctt ttggtcgcgt gcgcattgct aaactgaagg gcaccaatga ctattatgcg gtgaagtgct tgaagaagcg ggagatttta aagatgaagc aggtccaaca catcagccaa gaaaagcaaa ttttaatgga gctttcacac ccgttcattg tgaacatgat gtgttccttt caggatgatt gccgggtgta ctttgtgttg gagtttgtgg tgggcgggga aatgtttact caccttcgct ccgccggtcg gtttccaaac gatgtagcaa agttctacca cgcggagatt gtgcttgcct tcgaatacct gcactcgaag gacatcatct accgtgacct gaagccggag aaccttctgc tggacagcaa gggccacgtg aaggtgactg actttgggtt tgcgaagaag gtgccggaac gtaccttcac gctgtgtggg acaccggagt accttgcgcc cgangtgatt cagagcaagg gtcatgggaa ggccgtggac tggtggacga tgggcgttct gctgtacgaa ttcattgctg ggtatcctcc gttttacgac gacncgccgt tccgcacgta cgagaagatc ctctctgggc gcttcaagtt tccnagttgg tttgatgcgc gtgcgcgggn ncttgtgang gggcttctgn agnaccacac gaancgttnn ggcncactca ngggngngta nngnngtnan aatcnncnnn anntnnnnnn ncnnnnggna naantngnnn gnnnnatact ncnnnncnna tncnnnnnnn nnnnnnannn nnnnnnnnnn nnnannntnn nnnnnnnnan nnnnnnnnnn ng
Expected Protein Sequence
MAHHHHHHMT SFPHFVSFSY MCIYMYTSTN GHFSFLLKEP TQIILTVFLK NRHWRIAKRE TTVINKMSGE ANYEKPDTSN WKLADFEMGD TLGTGSFGRV RIAKLKGTND YYAVKCLKKR EILKMKQVQH ISQEKQILME LSHPFIVNMM CSFQDDRRVY FVLEFVVGGE MFTHLRSAGR FPNDVAKFYH AEIVLAFEYL HSKDIIYRDL KPENLLLDSK GHVKVTDFGF AKKVPERTFT LCGTPEYLAP EVIQSKGHGK AVDWWTMGVL LYEFIAGYPP FYDDTPFRTY EKILSGRFKF PSWFDARARD LVKGLLQTDH TKRLGTLKGG VADVKNHPYF HGANWEKLYA RYYPAPIPVK AKSPGDTSNF ERYPESQEDR AVPLTATQQA EFIGF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactt catttcccca ctttgtttct ttttcttata tgtgtatata tatgtataca tctacgaatg ggcatttttc cttccttcta aaagagccca cacagataat tttaacagta tttttgaaaa ataggcactg gcgaattgcc aagagggaga cgacagttat aaacaaaatg tcaggggagg caaattatga aaagccggac acctcaaatt ggaagttagc agactttgag atgggcgaca cgttagggac gggttctttt ggtcgcgtgc gcattgctaa actgaagggc accaatgact attatgcggt gaagtgcttg aagaagcggg agattttaaa gatgaagcag gtccaacaca tcagccaaga aaagcaaatt ttaatggagc tttcacaccc gttcattgtg aacatgatgt gttcctttca ggatgatcgc cgggtgtact ttgtgttgga gtttgtggtg ggcggggaaa tgtttactca ccttcgctcc gccggtcggt ttccaaacga tgtagcaaag ttctaccacg cggagattgt gcttgccttc gaatacctgc actcgaagga catcatctac cgtgacctga agccggagaa ccttctgctg gacagcaagg gccacgtgaa ggtgactgac tttgggtttg cgaagaaggt gccggaacgt accttcacgc tgtgtgggac accggagtac cttgcgcccg aggtgattca gagcaagggt catgggaagg ccgtggactg gtggacgatg ggcgttctgc tgtacgaatt tattgctggg tatcctccgt tttacgacga cacgccgttc cgcacgtacg agaagatcct ctctgggcgc ttcaagtttc cgagttggtt tgatgcgcgt gcgcgggatc ttgtgaaggg gcttctgcaa acggaccaca cgaagcgttt gggcacactc aagggcggcg tagcggatgt gaagaatcac ccgtactttc acggtgccaa ctgggagaag ttgtatgcgc gatactaccc tgccccgatc cctgtgaagg caaagagccc tggcgacacg agtaactttg agcgttaccc tgaaagccag gaagatcgag cggtgccgct aaccgctacg caacaggcgg agtttattgg gttctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat