TrcrA.01471.b

aspartate aminotransferase, putative

CENTER ID: TrcrA.01471.b
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01471.b.B1.GE35170 full length 1 418
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053510945.70
RefSeq: XP_808313.1
UniProt: Q4D1Q4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSFFSAKIV SAKAPLELRR AASSFFASVP LGAPDSILGL SAEFQQDSHT PKVNLAVGVY RDDANRPFVL ESVKRSDTGS DMEYAPINGM RSFLKAAQKL CFGEDSRALR DGRVASCHTL GGTGALRIGG EMLHNFVNDC SNIYSSDVGY ANHAGIFKAA GITLPPYTYY SPATKGIDLP GMLKSLEAMP ERSVVLLHAC AHNPTGVDPT QNEWLQVVDV IKRRNLLPFV DMAYQGFATG DIDRDAFLPR CLVENVPNVI VAQSFSKNFG LYGLRCGALH MVIENPEEVG RVLSQYALLI RTMYSNPPIT GARIVDSILN SQELTALWKE ELRAMSGRMQ DVRRRLVKEL GECGSVLDWS HIERQIGMMS YTGLTKEQVE MLKKKHHIYM TLNGRAAISG LNSTNVSYVA KAFHDVSK
NT Sequence
atgcgctctt ttttctctgc caaaattgtt tccgcgaagg ccccactgga acttcgccgc gccgccagca gtttttttgc gtccgtcccc ctgggagcgc ctgactccat cttgggccta tcggctgagt tccagcagga ttcccacacc ccgaaggtca atcttgccgt tggcgtttac cgcgacgacg cgaaccggcc gtttgttctc gagtcggtta agaggtcgga taccggcagt gacatggaat acgcccccat caacggcatg cggtcattct tgaaggcggc gcaaaaactt tgcttcggtg aggactcccg cgcacttcgc gatggccgcg ttgcgagctg ccatacactt ggagggaccg gtgcactgcg tattggtggc gaaatgcttc acaattttgt gaacgactgc agcaatattt actcctccga cgttggctac gccaaccatg ccggcatctt caaggcggcc ggaataacac tgccgccgta cacgtactac agccccgcca cgaagggcat cgacctgccc ggcatgctca aatcactgga ggcgatgccc gaacgctctg ttgttcttct gcacgcctgc gcccataacc ctaccggggt ggaccccacg caaaacgagt ggttgcaggt ggtggacgtg attaagcgac gaaacctttt gcccttcgtg gatatggctt accaggggtt tgccaccggt gatattgacc gtgacgcctt cctgcctcgt tgcctcgtcg agaatgttcc gaacgtcatc gtggcacaat ccttttcgaa gaactttggt ttgtacggtc tccgctgtgg cgccttgcat atggttattg agaacccaga ggaggtgggg cgcgttttat cgcaatacgc tcttcttatt cgcaccatgt acagtaatcc tcccattact ggggcccgta ttgttgactc tattctgaac tcgcaggaac tcacggcact gtggaaagag gagcttaggg caatgtctgg tcgcatgcag gatgtgcgtc ggcgccttgt aaaggagctg ggtgagtgcg ggtcggttct tgattggtcg cacattgagc gccagatcgg catgatgtca tacacgggtc tcacgaagga acaagtggaa atgctgaaaa agaagcacca catctacatg accctcaatg gtcgtgcagc catctccgga ctgaacagca ccaatgtttc ttacgtggcg aaggcctttc acgatgtttc gaaataa
Details for TrcrA.01471.b.B1.GE35170
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHMR SFFSAKIVSA KAPLELRRAA SSFFASVPLG APDSILGLSA EFQQDSHTPK VNLAVGVYRD DANRPFVLES VKRSDTGSDM EYAPINGMRS FLKAAQKLCF GEDSRALRDG RVASCHTLGG TGALRIGGEM LHNFVNDCSN IYSSDVGYAN HAGIFKAAGI TLPPYTYYSP ATKGIDLPGM LKSLEAMPER SVVLLHACAH NPTGVDPTQN EWLQVVDVIK RRNLLPFVDM AYQGFATGDI DRDAFLPRCL VXNVPNVIVA QSFSKNFGLY GLRCGALHMV IENPEXVGRV LSQYALXIRT MYSNPXITGA XXVDSXLXXX XHXTXXXXXX XXXXXXXXXX XXXWXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
ttgtnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgcgctct tttttctctg ccaaaattgt ttccgcgaag gccccactgg aacttcgccg cgccgccagc agtttttttg cgtccgtccc cctgggagcg cctgactcca tcttgggcct atcggctgag ttccagcagg attcccacac cccgaaggtc aatcttgccg ttggcgttta ccgcgacgac gcgaaccggc cgtttgttct cgagtcggtt aagaggtcgg ataccggcag tgacatggaa tacgccccca tcaacggcat gcggtcattc ttgaaggcgg cgcaaaaact ttgcttcggt gaggactccc gcgcacttcg cgatggccgc gttgcgagct gccatacact tggagggacc ggtgcactgc gtattggtgg cgaaatgctt cacaattttg tgaacgactg cagcaatatt tactcctccg acgttggcta cgccaaccat gccggcatct tcaaggcggc cggaataaca ctgccgccgt acacgtacta cagccccgcc acgaagggca tcgacctgcc cggcatgctc aaatcactgg aggcgatgcc cgaacgctct gttgttcttc tgcacgcctg cgcccataac cctaccgggg tggaccccac gcaaaacgag tggttgcagg tggtggacgt gattaagcga cgaaaccttt tgcccttcgt ggatatggct taccaggggt ttgccaccgg tgatattgac cgtgacgcct tcctgcctcg ttgcctcgtc nagaatgttc cgaacgtcat cgtggcacaa tccttttcga agaactttgg tttgtacggt ctccgctgtg gcgccttgca tatggttatt gagaacccag aggangtggg gcgcgtttta tcgcaatacg ctcttcnnat tcgcaccatg tacagtaatc ctnccattac tggggccnnt anngttgact ctnttctgan ncncngnant cacngcactn gnangagnan ctnnngnnan nnctngncnn atgnnnnnnn nncnnnnnna ngnnnctggn nnnannnnng nnnnnnnnnn ntnnnnnncn cntnnnannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagannnan nnnngnnnnn naannnnnnn nnnc
Expected Protein Sequence
MAHHHHHHMR SFFSAKIVSA KAPLELRRAA SSFFASVPLG APDSILGLSA EFQQDSHTPK VNLAVGVYRD DANRPFVLES VKRSDTGSDM EYAPINGMRS FLKAAQKLCF GEDSRALRDG RVASCHTLGG TGALRIGGEM LHNFVNDCSN IYSSDVGYAN HAGIFKAAGI TLPPYTYYSP ATKGIDLPGM LKSLEAMPER SVVLLHACAH NPTGVDPTQN EWLQVVDVIK RRNLLPFVDM AYQGFATGDI DRDAFLPRCL VENVPNVIVA QSFSKNFGLY GLRCGALHMV IENPEEVGRV LSQYALLIRT MYSNPPITGA RIVDSILNSQ ELTALWKEEL RAMSGRMQDV RRRLVKELGE CGSVLDWSHI ERQIGMMSYT GLTKEQVEML KKKHHIYMTL NGRAAISGLN STNVSYVAKA FHDVSK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgct cttttttctc tgccaaaatt gtttccgcga aggccccact ggaacttcgc cgcgccgcca gcagtttttt tgcgtccgtc cccctgggag cgcctgactc catcttgggc ctatcggctg agttccagca ggattcccac accccgaagg tcaatcttgc cgttggcgtt taccgcgacg acgcgaaccg gccgtttgtt ctcgagtcgg ttaagaggtc ggataccggc agtgacatgg aatacgcccc catcaacggc atgcggtcat tcttgaaggc ggcgcaaaaa ctttgcttcg gtgaggactc ccgcgcactt cgcgatggcc gcgttgcgag ctgccataca cttggaggga ccggtgcact gcgtattggt ggcgaaatgc ttcacaattt tgtgaacgac tgcagcaata tttactcctc cgacgttggc tacgccaacc atgccggcat cttcaaggcg gccggaataa cactgccgcc gtacacgtac tacagccccg ccacgaaggg catcgacctg cccggcatgc tcaaatcact ggaggcgatg cccgaacgct ctgttgttct tctgcacgcc tgcgcccata accctaccgg ggtggacccc acgcaaaacg agtggttgca ggtggtggac gtgattaagc gacgaaacct tttgcccttc gtggatatgg cttaccaggg gtttgccacc ggtgatattg accgtgacgc cttcctgcct cgttgcctcg tcgagaatgt tccgaacgtc atcgtggcac aatccttttc gaagaacttt ggtttgtacg gtctccgctg tggcgccttg catatggtta ttgagaaccc agaggaggtg gggcgcgttt tatcgcaata cgctcttctt attcgcacca tgtacagtaa tcctcccatt actggggccc gtattgttga ctctattctg aactcgcagg aactcacggc actgtggaaa gaggagctta gggcaatgtc tggtcgcatg caggatgtgc gtcggcgcct tgtaaaggag ctgggtgagt gcgggtcggt tcttgattgg tcgcacattg agcgccagat cggcatgatg tcatacacgg gtctcacgaa ggaacaagtg gaaatgctga aaaagaagca ccacatctac atgaccctca atggtcgtgc agccatctcc ggactgaaca gcaccaatgt ttcttacgtg gcgaaggcct ttcacgatgt ttcgaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at