SSGCID: Target Page

TrcrA.01471.a

aspartate aminotransferase, putative

CENTER ID:	TrcrA.01471.a
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.01471.a.B1.GE35169	full length		1	404

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053503841.70
RefSeq:	XP_807788.1
UniProt:	Q4D080

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAIRCLWNNI AALPADPIFS ASLVAKKAPE PKADLIIGAY RDAEGHPYPL NVVRKAEQRL LEMNADKEYL PMSGYAPFIE ESLKIAYGDS VARENVVGIQ GLSGTGSLSI GACFLARVLS RDTPVYISDP TWPNHYAVMA AANLTDLRKY RYYDNAKRCI DFDGLLEDLN GAPEGSIVIL HACAHNPTGM DPTHEQWAKI LEVFQARRLI PFFDSAYQGY ATGSLDNDAY SIRLFARQGM EMLLAQSYSK NMGLYAERVG VCSIVTANPK KAPLIKSQLE TIVRSQYSTP PAHGARVAYL VLSDPELRAG WEQELRVMST RVLEMRQALY DGLKRLGTPG SWEHIIQQVG MFSYLGLTKA QCEKLIERRV FVLPSGRANM AGLTKRSVEL LVKGIDEVVR TVTE

NT Sequence

atggcgatcc gatgcctctg gaacaacatt gctgccctcc cagcagaccc cattttttct gcttctttgg ttgcgaagaa agcaccagaa ccaaaagcag acctcattat tggcgcctac cgtgatgcgg aaggccaccc atacccccta aatgttgtaa gaaaagcaga acaacgcctt ctggaaatga atgccgacaa ggagtaccta ccaatgtcag ggtatgcgcc cttcattgaa gagtctttga agattgctta tggggacagt gtggcgcgtg aaaatgtggt gggcatacag ggactgagcg gcacagggtc actcagtatt ggagcgtgtt tcctcgcccg tgtactctcg cgcgacactc ctgtgtacat ttccgatccg acgtggccaa accactacgc tgttatggcc gcggcaaatc tgaccgactt acgcaagtac cgttactacg acaacgccaa acgttgcatt gactttgatg gtcttttaga agacctcaac ggggcgccgg agggcagtat tgtgatcctg catgcctgtg cacataatcc gacaggtatg gacccgaccc acgaacaatg ggccaaaatt ttggaggttt tccaggcgcg tcgtctcatt cccttctttg actctgcata tcagggatat gccaccggaa gccttgataa tgacgcctat tcgattcgtc tctttgcacg acaagggatg gaaatgcttc ttgcacagtc ctattcaaag aatatgggcc tgtacgcaga gcgtgtgggt gtctgctcca ttgtcacagc caacccaaag aaggcaccgt tgattaagtc gcagcttgaa acgatcgtac gtagccaata ttccactcca ccggctcacg gtgctcgagt ggcgtacctc gtcttgagcg accctgaact tcgtgcaggg tgggagcagg agttgcgagt aatgtcgacg cgtgtgctcg aaatgcgcca agctttgtac gatggcctca agagactcgg tacccctggc tcctgggaac atattattca gcaggttgga atgttttcat atcttggtct cacgaaggcc cagtgtgaaa aacttattga aaggcgtgtc tttgtactcc catccggacg tgcaaacatg gctggtctaa caaaaaggtc agtggaactg cttgtaaaag gcatcgacga ggtggtaaga accgtcacgg aatga

Details for TrcrA.01471.a.B1.GE35169

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA IRCLWNNIAA LPADPIFSAS LVAKKAPEPK ADLIIGAYRD AEGHPYPLNV VRKAEQRLLE MNADKEYLPM SGYAPFIEES LKIAYGDSVA RENVVGIQGL SGTGSLSIGA CFLARVLSRD TPVYISDPTW PNHYAVMAAA NLTDLRKYRY YDNAKRCIDF DGLLEDLNGA PEGSIVILHA CAHNPTGMDP THEQWAKILE VFQARRLIPF FDSAYQGYAT GSLDNDAYSI RLFARQGMEM LLAQSYSKNM GLYAERVGVC SIVTANPKKA PLIKSQLETI VRSQYSTPPA HGARVAYLVL SDPELRAGWE QELRVMSTRV LEMRQALYDG LKRLGTPGSW EHIIQQVGMF SYLGLTKAQC EKLIERRVFV LPSGRANMAG LTKRSVELLV KGIDEVVRTV TE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga tccgatgcct ctggaacaac attgctgccc tcccagcaga ccccattttt tctgcttctt tggttgcgaa gaaagcacca gaaccaaaag cagacctcat tattggcgcc taccgtgatg cggaaggcca cccatacccc ctaaatgttg taagaaaagc agaacaacgc cttctggaaa tgaatgccga caaggagtac ctaccaatgt cagggtatgc gcccttcatt gaagagtctt tgaagattgc ttatggggac agtgtggcgc gtgaaaatgt ggtgggcata cagggactga gcggcacagg gtcactcagt attggagcgt gtttcctcgc ccgtgtactc tcgcgcgaca ctcctgtgta catttccgat ccgacgtggc caaaccacta cgctgttatg gccgcggcaa atctgaccga cttacgcaag taccgttact acgacaacgc caaacgttgc attgactttg atggtctttt agaagacctc aacggggcgc cggagggcag tattgtgatc ctgcatgcct gtgcacataa tccgacaggt atggacccga cccacgaaca atgggccaaa attttggagg ttttccaggc gcgtcgtctc attcccttct ttgactctgc atatcaggga tatgccaccg gaagccttga taatgacgcc tattcgattc gtctctttgc acgacaaggg atggaaatgc ttcttgcaca gtcctattca aagaatatgg gcctgtacgc agagcgtgtg ggtgtctgct ccattgtcac agccaaccca aagaaggcac cgttgattaa gtcgcagctt gaaacgatcg tacgtagcca atattccact ccaccggctc acggtgctcg agtggcgtac ctcgtcttga gcgaccctga acttcgtgca gggtgggagc aggagttgcg agtaatgtcg acgcgtgtgc tcgaaatgcg ccaagctttg tacgatggcc tcaagagact cggtacccct ggctcctggg aacatattat tcagcaggtt ggaatgtttt catatcttgg tctcacgaag gcccagtgtg aaaaacttat tgaaaggcgt gtctttgtac tcccatccgg acgtgcaaac atggctggtc taacaaaaag gtcagtggaa ctgcttgtaa aaggcatcga cgaggtggta agaaccgtca cggaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat