SSGCID: Target Page

TrcrA.01451.b

protein kinase, putative,cdc2-related related kinase, putative

CENTER ID:	TrcrA.01451.b
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.01451.b.B2.GE35168	mature protein		17	339

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053510817.10
RefSeq:	XP_807171.1
UniProt:	Q4CYH2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIFGCGGILA SPEPIFCVPF IFSRCRRMEG RGGEEETRRE RWSLEMDRFI VGSPIGEGRF GVVRAAVVKE SGVPAAIKFV TTPRLDEGIP HPTAREPLVA MRLAHPFVIR KYEVVPCVCS LALVMERCKT DLATVLSGRT ASNPLPALEA KSLFKMLLLA LTYIHSEGVL HRDVKPSNCF LTAEGELRLG DFGLSRVWDT EASMTHEVVS RWYRAPELLL GQRHYGPEID VWSSGCVFAE LLRGHGGAFF AGDGDIWQLS RIFDVLGTPT PTSWPSAVLL PDWGKVHFEP KRPLHLREFF PDASECALDL LRRLLCLDPQ KRLAAAGALR HAYFSEYPT

NT Sequence

atgatctttg ggtgcggagg gattttggca tcaccggagc caattttttg tgttccattt atattttcac gatgccgtag aatggaaggg aggggtggag aagaggaaac aagaagagag aggtggtcgc ttgaaatgga tcgttttatt gttggatccc caattggtga gggccgcttt ggcgtcgttc gtgctgcagt tgttaaggaa tctggagtgc cagccgcaat caaattcgtc acaactccac gtctcgatga gggtattccg caccccacag cgagggaacc actcgttgca atgcgattgg cgcatccttt tgttattcgg aaatatgagg tggttccgtg tgtctgctcc ttggcgttag tgatggagcg ttgcaagact gacttggcaa cagtcctctc ggggagaact gccagcaatc ctctgcccgc gttagaggca aagtctttat ttaagatgct tcttttggcg ttaacgtaca tacacagcga gggtgttctt catcgagatg tgaaaccttc aaattgtttc ttgactgcgg agggggaact acgcctcggc gactttggac tcagtcgtgt atgggatacg gaagcatcca tgactcacga agtcgtttcg aggtggtacc gggctcccga gttacttctg ggccagcgac actacggacc cgaaattgat gtgtggtcat cagggtgcgt atttgcggaa cttctacggg ggcacggtgg tgcctttttt gcaggcgatg gagatatttg gcagttgtca cgaatatttg atgtattagg gacaccgacg cccacatcgt ggccatcggc ggtgctgctt cccgattggg gaaaggtgca ctttgagccg aagcgtcctc ttcatctccg tgaatttttc ccggatgcct ctgaatgtgc attggatttg ttgcgaagat tactttgcct ggatcctcag aagcgtttgg ctgcggctgg cgcccttcgt cacgcctact ttagtgaata cccaacgtga

Details for TrcrA.01451.b.B2.GE35168

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	37kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMC VPFIFSRCRR MEGRGGEEET RRERWSLEMD RFIVGSPIGE GRFGVVRAAV VKESGVPAAI KFVTTPRLDE GIPHPTAREP LVAMRLAHPF VIRKYEVVPC VCSLALVMER CKTDLATVLS GRTASNPLPA LEAKSLFKML LLALTYIHSE GVLHRDVKPS NCFLTAEGEL RLGDFGLSRV WDTEASMTHE VVSRWYRAPE LLLGQRHYGP EIDVWSSGCV FAELLRGHGG AFFAGDGDIW QLSRIFDVLG TPTPTSWPSA VLLPDWGKVH FEPKRPLHLR EFFPDASECA LDLLRRLLCL DPQKRLAAAG ALRHAYFSEY PT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgtg ttccatttat attttcacga tgccgtagaa tggaagggag gggtggagaa gaggaaacaa gaagagagag gtggtcgctt gaaatggatc gttttattgt tggatcccca attggtgagg gccgctttgg cgtcgttcgt gctgcagttg ttaaggaatc tggagtgcca gccgcaatca aattcgtcac aactccacgt ctcgatgagg gtattccgca ccccacagcg agggaaccac tcgttgcaat gcgattggcg catccttttg ttattcggaa atatgaggtg gttccgtgtg tctgctcctt ggcgttagtg atggagcgtt gcaagactga cttggcaaca gtcctctcgg ggagaactgc cagcaatcct ctgcccgcgt tagaggcaaa gtctttattt aagatgcttc ttttggcgtt aacgtacata cacagcgagg gtgttcttca tcgagatgtg aaaccttcaa attgtttctt gactgcggag ggggaactac gcctcggcga ctttggactc agtcgtgtat gggatacgga agcatccatg actcacgaag tcgtttcgag gtggtaccgg gctcccgagt tacttctggg ccagcgacac tacggacccg aaattgatgt gtggtcatca gggtgcgtat ttgcggaact tctacggggg cacggtggtg ccttttttgc aggcgatgga gatatttggc agttgtcacg aatatttgat gtattaggga caccgacgcc cacatcgtgg ccatcggcgg tgctgcttcc cgattgggga aaggtgcact ttgagccgaa gcgtcctctt catctccgtg aatttttccc ggatgcctct gaatgtgcat tggatttgtt gcgaagatta ctttgcctgg atcctcagaa gcgtttggct gcggctggcg cccttcgtca cgcctacttt agtgaatacc caacgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat