TrcrA.01427.a

inorganic pyrophosphatase, putative

CENTER ID: TrcrA.01427.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01427.a.B1.GE35165 full length 1 276
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053508153.820
RefSeq: XP_822125.1
UniProt: Q4E611

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRGTRIVRCA AGLSLALPRW RRQEVGAPST HAWRMFFTSD SVPVTEARTE PAMPTTGMRS AWHDLSLHPA ADPSIVTFVC EIPKGTRAKV ELQKEEPHNP FAQDVHKKKE GKPLRFYTYG DIPFNYGFAP QTWEDPLLVD ADTKCTGDGD PIDIVEVSDS PLPMGSIWAV RVLGVLGLID EGETDWKIIA ETLRPEGKMY ESLDKIPQEL RDTIVRWMRD YKTTDGKKRN ELAFNGELRG AEEALHVIRA CSRQYATLID GNAQNPGYWL RYCQDA
NT Sequence
atgcgtggta cccgcattgt tcggtgcgct gctgggctta gcctggcgct gccaaggtgg agacggcagg aagttggggc cccaagcacg catgcatggc gcatgttctt taccagcgac tcggttccgg tgacggaggc acggacggaa cccgcgatgc cgacgacggg gatgcgctct gcgtggcatg atctcagcct gcatcctgcc gcggatccat ccatcgtcac gtttgtctgt gagatcccaa agggaactcg ggcgaaggtg gagttgcaga aggaggaacc gcacaacccc tttgcacagg atgttcacaa aaagaaggag gggaaaccgc tgcgttttta cacgtacggt gacattccgt tcaactacgg ctttgctcca caaacatggg aggatccgtt gttggtggac gcggacacaa aatgcactgg cgacggagat cccatagaca ttgtggaagt ctccgattcc cctctcccta tgggctcgat ttgggctgtg cgcgtgcttg gcgtgttggg gcttattgat gagggtgaga cggactggaa aatcatagcg gaaacgctgc gacctgaggg caaaatgtac gaaagccttg ataaaattcc ccaagaactg agggacacga ttgtgcggtg gatgcgtgat tacaagacaa ctgatggaaa gaagcggaat gagttggcgt ttaatggtga actgcgtgga gccgaggagg ccttacacgt gattagggcg tgctcacgcc agtacgccac tcttatcgat ggcaacgctc aaaaccccgg ctattggctg cgttattgcc aggatgcgtg a
Details for TrcrA.01427.a.B1.GE35165
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR GTRIVRCAAG LSLALPRWRR QEVGAPSTHA WRMFFTSDSV PVTEARTEPA MPTTGMRSAW HDLSLHPAAD PSIVTFVCEI PKGTRAKVEL QKEEPHNPFA QDVHKKKEGK PLRFYTYGDI PFNYGFAPQT WEDPLLVDAD TKCTGDGDPI DIVEVSDSPL PMGSIWAVRV LGVLGLIDEG ETDWKIIAET LRPEGKMYES LDKIPQELRD TIVRWMRDYX TTDGKKRNEL AFNGELRGAX EALHVIRACS RQYATLIDGN AQNPGYWLRY CQDA
Validated NT Sequence
attttgtnnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatgcgt ggtacccgca ttgttcggtg cgctgctggg cttagcctgg cgctgccaag gtggagacgg caggaagttg gggccccaag cacgcatgca tggcgcatgt tctttaccag cgactcggtt ccggtgacgg aggcacggac ggaacccgcg atgccgacga cggggatgcg ctctgcgtgg catgatctca gcctgcatcc tgccgcggat ccatccatcg tcacgtttgt ctgtgagatc ccaaagggaa ctcgggcgaa ggtggagttg cagaaggagg aaccgcacaa cccctttgca caggatgttc acaaaaagaa ggaggggaaa ccgctgcgtt tttacacgta cggtgacatt ccgttcaact acggctttgc tccacaaaca tgggaggatc cgttgttggt ggacgcggac acaaaatgca ctggcgacgg agatcccata gacattgtgg aagtctccga ttcccctctc cctatgggct cgatttgggc tgtgcgcgtg cttggcgtgt tggggcttat tgatgagggt gagacggact ggaaaatcat agcggaaacg ctgcgacctg agggcaaaat gtacgaaagc cttgataaaa ttccccaaga actgagggac acgattgtgc ggtggatgcg tgattacnag acaactgatg gaaagaagcg gaatgagttg gcgtttaatg gtgaactgcg tggagccgan gaggccttac acgtgattag ggcgtgctca cgccagtacg ccactcttat cgatggcaac gctcaaaacc ccggctattg gctgcgttat tgccaggatg cgtaagtgag taagatagna tcnnnnnnnn nnncccnnnn nnnnnnnnnn nnnnnnnnnn nnnt
Expected Protein Sequence
MAHHHHHHMR GTRIVRCAAG LSLALPRWRR QEVGAPSTHA WRMFFTSDSV PVTEARTEPA MPTTGMRSAW HDLSLHPAAD PSIVTFVCEI PKGTRAKVEL QKEEPHNPFA QDVHKKKEGK PLRFYTYGDI PFNYGFAPQT WEDPLLVDAD TKCTGDGDPI DIVEVSDSPL PMGSIWAVRV LGVLGLIDEG ETDWKIIAET LRPEGKMYES LDKIPQELRD TIVRWMRDYK TTDGKKRNEL AFNGELRGAE EALHVIRACS RQYATLIDGN AQNPGYWLRY CQDA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg gtacccgcat tgttcggtgc gctgctgggc ttagcctggc gctgccaagg tggagacggc aggaagttgg ggccccaagc acgcatgcat ggcgcatgtt ctttaccagc gactcggttc cggtgacgga ggcacggacg gaacccgcga tgccgacgac ggggatgcgc tctgcgtggc atgatctcag cctgcatcct gccgcggatc catccatcgt cacgtttgtc tgtgagatcc caaagggaac tcgggcgaag gtggagttgc agaaggagga accgcacaac ccctttgcac aggatgttca caaaaagaag gaggggaaac cgctgcgttt ttacacgtac ggtgacattc cgttcaacta cggctttgct ccacaaacat gggaggatcc gttgttggtg gacgcggaca caaaatgcac tggcgacgga gatcccatag acattgtgga agtctccgat tcccctctcc ctatgggctc gatttgggct gtgcgcgtgc ttggcgtgtt ggggcttatt gatgagggtg agacggactg gaaaatcata gcggaaacgc tgcgacctga gggcaaaatg tacgaaagcc ttgataaaat tccccaagaa ctgagggaca cgattgtgcg gtggatgcgt gattacaaga caactgatgg aaagaagcgg aatgagttgg cgtttaatgg tgaactgcgt ggagccgagg aggccttaca cgtgattagg gcgtgctcac gccagtacgc cactcttatc gatggcaacg ctcaaaaccc cggctattgg ctgcgttatt gccaggatgc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat