SSGCID: Target Page

TrcrA.01252.b

protein kinase, putative,cdc2-related related kinase, putative

CENTER ID:	TrcrA.01252.b
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.01252.b.B1.GE35148	full length		1	312

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053503607.10
RefSeq:	XP_805372.1
UniProt:	Q4CTB9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEGRGGEEKK RIERWSLDMD RFIVGSPIGE GRFGVVRAAV VKESGVPAAI KFITTPRLDE GIPHPTAREL LVALRLVHPF VIRTYEVVPC GCSLALVMER RKTDLATVLS GRTASNPLPA LEAKSLFKML LLALAYIHSE GVLHRDVKHS NCFLTAEGEL RLGDFGLSRL WDTEASMTHE VVSRWYRAPE LLLGQRHYGP EIDVWASGCV FAELLRGHGG AFFAGDGDIW QLSRIFDVLG TPTPTSWPSA VLLPDWGKAH FEPKRPLPLR EFFPDASECA LDLLRRLLCL DPQKRLTAAG ALRHAYFSEY PT

NT Sequence

atggaaggga ggggtggaga agagaaaaaa agaatagaga ggtggtcgct tgacatggat cgttttattg ttggatcccc aattggtgag ggacgctttg gcgtcgttcg tgctgcagtt gttaaggaat ctggagtgcc agccgcaatc aaattcatca caactccacg tctcgatgag ggtattccgc accccacagc gagggaacta ctcgttgcat tgcgattggt gcatcctttt gttattcgga catatgaggt ggttccgtgt ggctgctcct tggcgttagt gatggagcgt cgcaagactg acttggcaac agtcctctca gggagaactg ccagcaatcc tctgcccgcg ttagaggcaa agtctttatt taaaatgctt cttttggcgt tagcgtacat acacagcgaa ggtgttcttc atcgagatgt gaaacattca aattgtttct tgactgcgga gggggaacta cgcctcggcg actttggact aagtcgttta tgggatacgg aagcatccat gactcacgaa gtcgtttcga ggtggtaccg ggctcccgag ttacttctgg gccagcgaca ctacggaccc gaaattgatg tgtgggcatc agggtgcgta tttgcggagc ttctacgggg gcacggtggt gccttttttg caggcgatgg agatatttgg cagttgtcac gaatatttga tgtattaggg acaccgacgc ccacatcgtg gccatcggcg gtgctgcttc ccgattgggg aaaggcgcac tttgagccga agcgtcctct tcctctccgt gaatttttcc cggatgcctc tgaatgtgca ttggatttgt tgcgaagatt actttgcctg gatcctcaga agcgtttgac tgcggctggc gcccttcgtc acgcctactt tagtgaatac ccaacgtga

Details for TrcrA.01252.b.B1.GE35148

HARVESTED ON:	4/24/2012
SEQUENCED ON:	4/24/2012
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME GRGGEEKKRI ERWSLDMDRF IVGSPIGEGR FGVVRAAVVK ESGVPAAIKF ITTPRLDEGI PHPTARELLV ALRLVHPFVI RTYEVVPCGC SLALVMERRK TDLATVLSGR TASNPLPALE AKSLFKMLLL ALAYIHSEGV LHRDVKHSNC FLTAEGELRL GDFGLSRLWD TEASMTHEVV SRWYRAPELL LGQRHYGPEI DVWASGCVFA ELLRGHGGAF FAGDGDIWQL SRIFDVLGTP TPTSWPSAVL LPDWGKAHFE PKRPLPLREF FPDASECALD LLRXLLCLDP QKRLTAAGAL RHAYFXXXPT

Validated NT Sequence

tttntnnnnn ttaagaagga ganataccat ggctcaccac caccaccacc atatggaagg gaggggtgga gaagagaaaa aaagaataga gaggtggtcg cttgacatgg atcgttttat tgttggatcc ccaattggtg agggacgctt tggcgtcgtt cgtgctgcag ttgttaagga atctggagtg ccagccgcaa tcaaattcat cacaactcca cgtctcgatg agggtattcc gcaccccaca gcgagggaac tactcgttgc attgcgattg gtgcatcctt ttgttattcg gacatatgag gtggttccgt gtggctgctc cttggcgtta gtgatggagc gtcgcaagac tgacttggca acagtcctct cagggagaac tgccagcaat cctctgcccg cgttagaggc aaagtcttta tttaaaatgc ttcttttggc gttagcgtac atacacagcg aaggtgttct tcatcgagat gtgaaacatt caaattgttt cttgactgcg gagggggaac tacgcctcgg cgactttgga ctaagtcgtt tatgggatac ggaagcatcc atgactcacg aagtcgtttc gaggtggtac cgggctcccg agttacttct gggccagcga cactacggac ccgaaattga tgtgtgggca tcagggtgcg tatttgcgga gcttctacgg gggcacggtg gtgccttttt tgcaggcgat ggagatattt ggcagttgtc acgaatattt gatgtattag ggacaccgac gcccacatcg tggccatcgg cggtgctgct tcccgattgg ggaaaggcgc actttgagcc gaagcgtcct cttcctctcc gtgaattttt cccggatgcc tctgaatgtg cattggattt gttgcgaana ttactttgcc tggatcctca gaagcgtttg actgcngctg gcgcccttcg tcacgcctac tttantgann acccnacgta agtnnnnann nnnnnnnnnn nnnnnccccc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag ggaggggtgg agaagagaaa aaaagaatag agaggtggtc gcttgacatg gatcgtttta ttgttggatc cccaattggt gagggacgct ttggcgtcgt tcgtgctgca gttgttaagg aatctggagt gccagccgca atcaaattca tcacaactcc acgtctcgat gagggtattc cgcaccccac agcgagggaa ctactcgttg cattgcgatt ggtgcatcct tttgttattc ggacatatga ggtggttccg tgtggctgct ccttggcgtt agtgatggag cgtcgcaaga ctgacttggc aacagtcctc tcagggagaa ctgccagcaa tcctctgccc gcgttagagg caaagtcttt atttaaaatg cttcttttgg cgttagcgta catacacagc gaaggtgttc ttcatcgaga tgtgaaacat tcaaattgtt tcttgactgc ggagggggaa ctacgcctcg gcgactttgg actaagtcgt ttatgggata cggaagcatc catgactcac gaagtcgttt cgaggtggta ccgggctccc gagttacttc tgggccagcg acactacgga cccgaaattg atgtgtgggc atcagggtgc gtatttgcgg agcttctacg ggggcacggt ggtgcctttt ttgcaggcga tggagatatt tggcagttgt cacgaatatt tgatgtatta gggacaccga cgcccacatc gtggccatcg gcggtgctgc ttcccgattg gggaaaggcg cactttgagc cgaagcgtcc tcttcctctc cgtgaatttt tcccggatgc ctctgaatgt gcattggatt tgttgcgaag attactttgc ctggatcctc agaagcgttt gactgcggct ggcgcccttc gtcacgccta ctttagtgaa tacccaacgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat