TrcrA.01252.a

protein kinase, putative

CENTER ID: TrcrA.01252.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01252.a.B1.GE35147 full length 1 432
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01252.a.B1.PS01520 full length 1 432

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053508173.170
RefSeq: XP_818274.1
UniProt: Q4DV69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKEAQKVLK GAAYTEASPI SEVSHQKASA SKAFLENHYR GMLRETRSGG SARPVGPRQR EPCAEDFVLL KLIGRGAFGE VYVCHRKDDE EKQLYALKRM RKTDMVRKKQ TVHVRSEKDV MAEAAAKNEW VVRLHLSFQD ETYLYMVMEY MPGGDMISWL CLKQKFDLES TRFYIAELSA AVASVHEMCF VHRDIKPDNI LLSASGHIKL TDFGLSKRFA KAGEELLDLE ESALPESTTL SEMAGKPDTA SPAERHGVVH FRDRRMFESI VGSPGYIAPE ILLRQRYGIA CDWWSVGVIM YEMLYGIPPF FSPNPNSTCY KITHWREYLL YPDNCDVPED AVDFMQRLMC DPKDRLDFSG IKKHAFFYGI DWERLRETKA AFVPALTNLL DTKYFPEIDD GDKVQRGEER NIVKEYDPHG VMFADYKFIP SR
NT Sequence
atgaagaaag aggcacagaa ggtgctgaaa ggcgccgcgt acacggaggc tagcccaatt tcagaagtct cccatcagaa ggcgtcggcc agcaaggctt ttcttgagaa tcactaccgt ggtatgttgc gggagacgcg tagtggtggt agtgcacgcc cggttggccc gcgacaacgg gagccgtgtg cggaggattt tgttctcttg aaactcattg gcaggggcgc ctttggtgag gtgtacgttt gccaccgtaa ggatgatgaa gaaaagcagc tgtatgccct taagcggatg cggaagacag atatggttcg caaaaagcag acggttcatg tgaggtcaga gaaggacgtc atggcggagg cagctgcgaa gaatgaatgg gtggtgcgtt tgcaccttag cttccaggat gagacttatc tctacatggt gatggaatac atgccgggag gcgacatgat ctcatggctt tgtttaaaac aaaagtttga cttggagagc acgcggttct acattgcgga actttccgct gctgtcgcaa gtgttcacga aatgtgtttt gtacaccgtg acattaaacc agacaacata ttgttgagcg ccagtggaca catcaaactt acggattttg gtctatccaa gcggtttgcg aaggcgggtg aggagcttct agatctcgaa gaaagtgcct tgccggaaag cacaacactc agtgaaatgg cagggaagcc agacaccgcg tcaccagccg agagacatgg tgtagtgcat ttccgcgatc gacgcatgtt tgagtctatt gtgggtagtc ccggctacat tgcgccggag atcttgctgc gtcagcgcta tggtatagcg tgcgactggt ggtctgttgg tgtcattatg tacgagatgt tgtacggtat cccgcccttc ttttcaccaa acccgaactc cacctgctac aagatcacac actggcgaga gtatctcctt taccctgaca actgtgacgt gcctgaagac gcggtggatt ttatgcagcg cctgatgtgc gaccccaaag atcgccttga ctttagcggc atcaagaaac acgccttttt ttatggcatc gactgggaac gtttgcgtga gacgaaagcc gcttttgtac cggcgctgac caatttgctt gacacaaagt acttccccga gattgacgac ggggacaaag tgcaacgggg tgaggagcgc aacattgtga aggaatatga cccacacggc gtcatgtttg cggattacaa gttcattcct tcacgatga
Details for TrcrA.01252.a.B1.GE35147
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 57
Validated AA Sequence
MLRETRSGGS ARPVGPRQRE PCAEDFVLLK LIGRGAFGEV YVCHRKDDEE KQLYALKRMR KTDMVRKKQT VHVRSEKDVM AEAAAKNEWV VRLHLSFQDE TYLYMVMEYM PGGDMISWLC LKQKFDLEST RFYIAELSAA VASVHEMCFV HRDIKPDNIL LSASGHIKLT DFGLSKRFAK AGEELLDLEE SALPESTTLS EMAGKPDTAS PAERHGVVHF RDRRMFESIV GSPGYIAPXX LLRQRYGYSV RLVV
Validated NT Sequence
cagaccacca gtcgcacgct atacccatag cgctgacgca gcaagnnntc nggcgcaatg tagccgggac tacccacaat agactcaaac atgcgtcgat cgcggaaatg cactacacca tgtctctcgg ctggtgacgc ggtgtctggc ttccctgcca tttcactgag tgttgtgctt tccggcaagg cactttcttc gagatctaga agctcctcac ccgccttcgc aaaccgcttg gatagaccaa aatccgtaag tttgatgtgt ccactggcgc tcaacaatat gttgtctggt ttaatgtcac ggtgtacaaa acacatttcg tgaacacttg cgacagcagc ggaaagttcc gcaatgtaga accgcgtgct ctccaagtca aacttttgtt ttaaacaaag ccatgagatc atgtcgcctc ccggcatgta ttccatcacc atgtagagat aagtctcatc ctggaagcta aggtgcaaac gcaccaccca ttcattcttc gcagctgcct ccgccatgac gtccttctct gacctcacat gaaccgtctg ctttttgcga accatatctg tcttccgcat ccgcttaagg gcatacagct gcttttcttc atcatcctta cggtggcaaa cgtacacctc accaaaggcg cccctgccaa tgagtttcaa gagaacaaaa tcctccgcac acggctcccg ttgtcgcggg ccaaccgggc gtgcactacc accactacgc gtctcccgca acataccacg gtagtgattc tcaagaaaag ccttgctggc cgacgccttc tgatgggaga cttctgaaat tgggctagcc tccgtgtacg cggcgccttt cagcaccttc tgtgcctctt t
Expected Protein Sequence
MAHHHHHHMK KEAQKVLKGA AYTEASPISE VSHQKASASK AFLENHYRGM LRETRSGGSA RPVGPRQREP CAEDFVLLKL IGRGAFGEVY VCHRKDDEEK QLYALKRMRK TDMVRKKQTV HVRSEKDVMA EAAAKNEWVV RLHLSFQDET YLYMVMEYMP GGDMISWLCL KQKFDLESTR FYIAELSAAV ASVHEMCFVH RDIKPDNILL SASGHIKLTD FGLSKRFAKA GEELLDLEES ALPESTTLSE MAGKPDTASP AERHGVVHFR DRRMFESIVG SPGYIAPEIL LRQRYGIACD WWSVGVIMYE MLYGIPPFFS PNPNSTCYKI THWREYLLYP DNCDVPEDAV DFMQRLMCDP KDRLDFSGIK KHAFFYGIDW ERLRETKAAF VPALTNLLDT KYFPEIDDGD KVQRGEERNI VKEYDPHGVM FADYKFIPSR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga aagaggcaca gaaggtgctg aaaggcgccg cgtacacgga ggctagccca atttcagaag tctcccatca gaaggcgtcg gccagcaagg cttttcttga gaatcactac cgtggtatgt tgcgggagac gcgtagtggt ggtagtgcac gcccggttgg cccgcgacaa cgggagccgt gtgcggagga ttttgttctc ttgaaactca ttggcagggg cgcctttggt gaggtgtacg tttgccaccg taaggatgat gaagaaaagc agctgtatgc ccttaagcgg atgcggaaga cagatatggt tcgcaaaaag cagacggttc atgtgaggtc agagaaggac gtcatggcgg aggcagctgc gaagaatgaa tgggtggtgc gtttgcacct tagcttccag gatgagactt atctctacat ggtgatggaa tacatgccgg gaggcgacat gatctcatgg ctttgtttaa aacaaaagtt tgacttggag agcacgcggt tctacattgc ggaactttcc gctgctgtcg caagtgttca cgaaatgtgt tttgtacacc gtgacattaa accagacaac atattgttga gcgccagtgg acacatcaaa cttacggatt ttggtctatc caagcggttt gcgaaggcgg gtgaggagct tctagatctc gaagaaagtg ccttgccgga aagcacaaca ctcagtgaaa tggcagggaa gccagacacc gcgtcaccag ccgagagaca tggtgtagtg catttccgcg atcgacgcat gtttgagtct attgtgggta gtcccggcta cattgcgccg gagatcttgc tgcgtcagcg ctatggtata gcgtgcgact ggtggtctgt tggtgtcatt atgtacgaga tgttgtacgg tatcccgccc ttcttttcac caaacccgaa ctccacctgc tacaagatca cacactggcg agagtatctc ctttaccctg acaactgtga cgtgcctgaa gacgcggtgg attttatgca gcgcctgatg tgcgacccca aagatcgcct tgactttagc ggcatcaaga aacacgcctt tttttatggc atcgactggg aacgtttgcg tgagacgaaa gccgcttttg taccggcgct gaccaatttg cttgacacaa agtacttccc cgagattgac gacggggaca aagtgcaacg gggtgaggag cgcaacattg tgaaggaata tgacccacac ggcgtcatgt ttgcggatta caagttcatt ccttcacgat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for TrcrA.01252.a.B1.PS01520
PURIFICATION DATe: 6/7/2012
CONCENTRATION: 34.13mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 110µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 57
Protocol Notes
notes unavailable
Validated AA Sequence
MLRETRSGGS ARPVGPRQRE PCAEDFVLLK LIGRGAFGEV YVCHRKDDEE KQLYALKRMR KTDMVRKKQT VHVRSEKDVM AEAAAKNEWV VRLHLSFQDE TYLYMVMEYM PGGDMISWLC LKQKFDLEST RFYIAELSAA VASVHEMCFV HRDIKPDNIL LSASGHIKLT DFGLSKRFAK AGEELLDLEE SALPESTTLS EMAGKPDTAS PAERHGVVHF RDRRMFESIV GSPGYIAPXX LLRQRYGYSV RLVV
Validated NT Sequence
cagaccacca gtcgcacgct atacccatag cgctgacgca gcaagnnntc nggcgcaatg tagccgggac tacccacaat agactcaaac atgcgtcgat cgcggaaatg cactacacca tgtctctcgg ctggtgacgc ggtgtctggc ttccctgcca tttcactgag tgttgtgctt tccggcaagg cactttcttc gagatctaga agctcctcac ccgccttcgc aaaccgcttg gatagaccaa aatccgtaag tttgatgtgt ccactggcgc tcaacaatat gttgtctggt ttaatgtcac ggtgtacaaa acacatttcg tgaacacttg cgacagcagc ggaaagttcc gcaatgtaga accgcgtgct ctccaagtca aacttttgtt ttaaacaaag ccatgagatc atgtcgcctc ccggcatgta ttccatcacc atgtagagat aagtctcatc ctggaagcta aggtgcaaac gcaccaccca ttcattcttc gcagctgcct ccgccatgac gtccttctct gacctcacat gaaccgtctg ctttttgcga accatatctg tcttccgcat ccgcttaagg gcatacagct gcttttcttc atcatcctta cggtggcaaa cgtacacctc accaaaggcg cccctgccaa tgagtttcaa gagaacaaaa tcctccgcac acggctcccg ttgtcgcggg ccaaccgggc gtgcactacc accactacgc gtctcccgca acataccacg gtagtgattc tcaagaaaag ccttgctggc cgacgccttc tgatgggaga cttctgaaat tgggctagcc tccgtgtacg cggcgccttt cagcaccttc tgtgcctctt t
Expressed Protein Sequence
MAHHHHHHMK KEAQKVLKGA AYTEASPISE VSHQKASASK AFLENHYRGM LRETRSGGSA RPVGPRQREP CAEDFVLLKL IGRGAFGEVY VCHRKDDEEK QLYALKRMRK TDMVRKKQTV HVRSEKDVMA EAAAKNEWVV RLHLSFQDET YLYMVMEYMP GGDMISWLCL KQKFDLESTR FYIAELSAAV ASVHEMCFVH RDIKPDNILL SASGHIKLTD FGLSKRFAKA GEELLDLEES ALPESTTLSE MAGKPDTASP AERHGVVHFR DRRMFESIVG SPGYIAPEIL LRQRYGIACD WWSVGVIMYE MLYGIPPFFS PNPNSTCYKI THWREYLLYP DNCDVPEDAV DFMQRLMCDP KDRLDFSGIK KHAFFYGIDW ERLRETKAAF VPALTNLLDT KYFPEIDDGD KVQRGEERNI VKEYDPHGVM FADYKFIPSR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGA AAGAGGCACA GAAGGTGCTG AAAGGCGCCG CGTACACGGA GGCTAGCCCA ATTTCAGAAG TCTCCCATCA GAAGGCGTCG GCCAGCAAGG CTTTTCTTGA GAATCACTAC CGTGGTATGT TGCGGGAGAC GCGTAGTGGT GGTAGTGCAC GCCCGGTTGG CCCGCGACAA CGGGAGCCGT GTGCGGAGGA TTTTGTTCTC TTGAAACTCA TTGGCAGGGG CGCCTTTGGT GAGGTGTACG TTTGCCACCG TAAGGATGAT GAAGAAAAGC AGCTGTATGC CCTTAAGCGG ATGCGGAAGA CAGATATGGT TCGCAAAAAG CAGACGGTTC ATGTGAGGTC AGAGAAGGAC GTCATGGCGG AGGCAGCTGC GAAGAATGAA TGGGTGGTGC GTTTGCACCT TAGCTTCCAG GATGAGACTT ATCTCTACAT GGTGATGGAA TACATGCCGG GAGGCGACAT GATCTCATGG CTTTGTTTAA AACAAAAGTT TGACTTGGAG AGCACGCGGT TCTACATTGC GGAACTTTCC GCTGCTGTCG CAAGTGTTCA CGAAATGTGT TTTGTACACC GTGACATTAA ACCAGACAAC ATATTGTTGA GCGCCAGTGG ACACATCAAA CTTACGGATT TTGGTCTATC CAAGCGGTTT GCGAAGGCGG GTGAGGAGCT TCTAGATCTC GAAGAAAGTG CCTTGCCGGA AAGCACAACA CTCAGTGAAA TGGCAGGGAA GCCAGACACC GCGTCACCAG CCGAGAGACA TGGTGTAGTG CATTTCCGCG ATCGACGCAT GTTTGAGTCT ATTGTGGGTA GTCCCGGCTA CATTGCGCCG GAGATCTTGC TGCGTCAGCG CTATGGTATA GCGTGCGACT GGTGGTCTGT TGGTGTCATT ATGTACGAGA TGTTGTACGG TATCCCGCCC TTCTTTTCAC CAAACCCGAA CTCCACCTGC TACAAGATCA CACACTGGCG AGAGTATCTC CTTTACCCTG ACAACTGTGA CGTGCCTGAA GACGCGGTGG ATTTTATGCA GCGCCTGATG TGCGACCCCA AAGATCGCCT TGACTTTAGC GGCATCAAGA AACACGCCTT TTTTTATGGC ATCGACTGGG AACGTTTGCG TGAGACGAAA GCCGCTTTTG TACCGGCGCT GACCAATTTG CTTGACACAA AGTACTTCCC CGAGATTGAC GACGGGGACA AAGTGCAACG GGGTGAGGAG CGCAACATTG TGAAGGAATA TGACCCACAC GGCGTCATGT TTGCGGATTA CAAGTTCATT CCTTCACGAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT